Rh6BG404400

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012254
Physical Location & Seq
Forward (+)
63150163 .. 63155665
5503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG404400.1

Sequence Viewer

Length: 564 bp
ATGAGGACGGTCATGTCGCGCTTGATCCATTCAAGATCATCACTTCCCAGGCCCTTTATGATGACTGCACCTGTAAATGGGAGGTATCTCTCAGGCGAATCTAATAAGGTTGATGAGCCCTTCAAAGCTGAGGAAGCAGAGACTATTCAAGCCCCCCCTCCTCCAACAGAGAAGTTACTTGTGTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCATTAGATCGTGGACTGTTTGTTGCTAGCCTTAGCAGGTCTGGTAGGTCAAAGTTACATGATTCATGGGCTAACAATGTGACCTGGTACCAAGGAAACCTTCTATCACCTGATTCATGGAAGGATGCTCTTGACGGTGTCACGTCTGTTATATCCTGCGTTGGTGGCTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCAAACATCAATGCAATTAGAGCTGCTTCTGAGCATGGTGTGAAAAGATTTGTGTATGTCTCTGCTGCTGATTTTGGCTTGGTAAATTACTTGCTGCGAGGATATTATGAGGGAAAGGTATCTTGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

187

Amino Acids

20.43

Weight (kDa)

9.01

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 166 2.5e-11 NmrA-like family
Epimerase PF01370 60 - 167 6.8e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 168 4.4e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 170 8.1e-18 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 252
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccII CGCG 1 cut(s) 19
AclWI GGATC 1 cut(s) 19
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 77
AflIII ACRYGT 2 cut(s) 211, 411
AgsI TTSAA 3 cut(s) 33, 124, 149
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 2 cut(s) 47, 308
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AluBI AGCT 2 cut(s) 128, 455
AluI AGCT 2 cut(s) 128, 455
Alw26I GTCTC 2 cut(s) 134, 496
AlwI GGATC 1 cut(s) 19
AoxI GGCC 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 455, 497, 526
AseI ATTAAT 1 cut(s) 423
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 1 cut(s) 324
AsuNHI GCTAGC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 120
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 442, 484, 513
BciT130I CCWGG 2 cut(s) 49, 310
BcoDI GTCTC 2 cut(s) 134, 496
BfaI CTAG 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 3 cut(s) 456, 498, 527
BlsI GCNGC 3 cut(s) 457, 499, 528
Bme1390I CCNGG 2 cut(s) 49, 310
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 2 cut(s) 314, 400
BmrFI CCNGG 2 cut(s) 49, 310
BmsI GCATC 1 cut(s) 340
BmtI GCTAGC 1 cut(s) 255
Bpu10I CCTNAGC 2 cut(s) 129, 257
BsaJI CCNNGG 2 cut(s) 47, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 77
BseBI CCWGG 2 cut(s) 49, 310
BseDI CCNNGG 2 cut(s) 47, 316
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 3 cut(s) 105, 120, 453
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 3 cut(s) 442, 484, 513
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 1 cut(s) 52
BshNI GGYRCC 1 cut(s) 312
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 2 cut(s) 134, 496
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 3 cut(s) 24, 35, 232
BspANI GGCC 1 cut(s) 52
BspCNI CTCAG 3 cut(s) 104, 121, 454
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 314, 400
BspMI ACCTGC 1 cut(s) 252
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 312
BssECI CCNNGG 2 cut(s) 47, 316
BssMI GATC 3 cut(s) 24, 35, 232
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 2 cut(s) 49, 310
Bst4CI ACNGT 3 cut(s) 10, 243, 362
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 91, 129, 257, 462
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 19
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 3 cut(s) 27, 38, 235
BstMAI GTCTC 2 cut(s) 134, 496
BstMBI GATC 3 cut(s) 24, 35, 232
BstMWI GCNNNNNNNGC 3 cut(s) 134, 390, 452
BstNI CCWGG 2 cut(s) 49, 310
BstNSI RCATGY 2 cut(s) 215, 415
BstSCI CCNGG 2 cut(s) 47, 308
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 3 cut(s) 442, 484, 513
BsuRI GGCC 1 cut(s) 52
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 355
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 253
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 51
CsiI ACCWGGT 1 cut(s) 308
Csp6I GTAC 1 cut(s) 313
CviAII CATG 7 cut(s) 13, 212, 284, 291, 342, 412, 467
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 4 cut(s) 91, 129, 257, 462
DpnI GATC 3 cut(s) 26, 37, 234
DpnII GATC 3 cut(s) 24, 35, 232
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 47, 308
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 7 cut(s) 16, 215, 287, 294, 345, 415, 470
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 442
FatI CATG 7 cut(s) 12, 211, 283, 290, 341, 411, 466
Fnu4HI GCNGC 3 cut(s) 456, 498, 527
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 3 cut(s) 456, 498, 527
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 20
GluI GCNGC 3 cut(s) 456, 498, 527
HaeIII GGCC 1 cut(s) 52
HhaI GCGC 1 cut(s) 21
Hin1II CATG 7 cut(s) 16, 215, 287, 294, 345, 415, 470
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HinfI GANTC 3 cut(s) 98, 287, 338
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 2 cut(s) 33, 356
Hpy8I GTNNAC 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 130, 335, 340
HpyCH4III ACNGT 3 cut(s) 10, 243, 362
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 3 cut(s) 68, 434, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 390, 452
HpyF3I CTNAG 4 cut(s) 91, 129, 257, 462
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 7 cut(s) 16, 215, 287, 294, 345, 415, 470
HspAI GCGC 1 cut(s) 19
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 24, 35, 232
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 3 cut(s) 442, 484, 513
LweI GCATC 1 cut(s) 340
MabI ACCWGGT 1 cut(s) 308
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 4 cut(s) 174, 279, 304, 364
MalI GATC 3 cut(s) 26, 37, 234
MboI GATC 3 cut(s) 24, 35, 232
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 3 cut(s) 403, 447, 517
MmeI TCCRAC 1 cut(s) 188
MnlI CCTC 6 cut(s) 75, 124, 168, 171, 524, 535
MseI TTAA 1 cut(s) 423
MspR9I CCNGG 2 cut(s) 49, 310
MvaI CCWGG 2 cut(s) 49, 310
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 3 cut(s) 134, 390, 452
NdeII GATC 3 cut(s) 24, 35, 232
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 7 cut(s) 16, 215, 287, 294, 345, 415, 470
NlaIV GGNNCC 2 cut(s) 314, 400
NmuCI GTSAC 2 cut(s) 304, 364
NspI RCATGY 2 cut(s) 215, 415
PciI ACATGT 2 cut(s) 211, 411
PfeI GAWTC 3 cut(s) 98, 287, 338
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 3 cut(s) 457, 499, 528
PscI ACATGT 2 cut(s) 211, 411
PshBI ATTAAT 1 cut(s) 423
Psp6I CCWGG 2 cut(s) 47, 308
PspGI CCWGG 2 cut(s) 47, 308
PspN4I GGNNCC 2 cut(s) 314, 400
PspPI GGNCC 1 cut(s) 51
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 3 cut(s) 456, 498, 527
Sau3AI GATC 3 cut(s) 24, 35, 232
Sau96I GGNCC 1 cut(s) 51
ScrFI CCNGG 2 cut(s) 49, 310
SduI GDGCHC 1 cut(s) 120
SexAI ACCWGGT 1 cut(s) 308
SfaNI GCATC 1 cut(s) 340
Sse9I AATT 3 cut(s) 403, 447, 517
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 47, 308
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 3 cut(s) 10, 243, 362
TaiI ACGT 1 cut(s) 371
TasI AATT 3 cut(s) 403, 447, 517
TfiI GAWTC 3 cut(s) 98, 287, 338
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseFI GTSAC 2 cut(s) 304, 364
TseI GCWGC 3 cut(s) 455, 497, 526
Tsp45I GTSAC 2 cut(s) 304, 364
TspDTI ATGAA 2 cut(s) 279, 330
Tth111I GACNNNGTC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 423
XceI RCATGY 2 cut(s) 215, 415
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.