pycom15g04250

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012254
Physical Location & Seq
Forward (+)
2560085 .. 2562934
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04250.1

Sequence Viewer

Length: 858 bp
ATGAGGACGGTCGTGTCGCGGTTGATCCAACCAAGATCATCACTTCCAAGGCTCTTTGTATCGGATGCACTGAAGAATGGTAGGTATCTAACAACAGACTCTAATAAGGTTGATGAACCCTTCAAAGTTGAGGAAGCTGAGACTGTCAACATTCCCCCTCCTCCAACTGATAAGTTGCTTGTCTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCATTGGGTCGGGGCTTGTCTGTCGCTAGCCTTAGCAGATCTGGTAGGTCAAAATTACATGATTCGTGGGCCAACACTGTGACCTGGCATCAAGGAAACCTTCTATCGCTTGAATCATTAAAGGATGCTTTCAATGGTGTCACGTCCGTTATATCCTGTGTTGGTGGGTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCCAACATCAATGCAATTAGAGTTGCCGCTGAACAAGGCGTGAAAAGATTTGTGTATATCTCTGCTGCTGATTTTGGTGTGGCAAATTACTTACTGCAGGGATATTATGAGGGAAAGAGAGCTGCAGAGACAGAGCTACTAACCAAATTCCCATATGGAGGAGTGATTTTGAGGCCTGGATTTATATATGGGACTCGAACTGTTGGGAGTGTGAAGATACCTCTTGGTGTAATTGGTTCTCCTCTTGAGATGCTATTTCAAAACACGAGACCCCTGAGCCAGCTGCCACTTGTTGGGCCTCTCTTTACTCCTCCAGTGAATGTAACCTCTGTGGCAAATGTTGCCGTTAGAGCAGCTACTGATCCTGTCTTCCCTCCTGGGATTGTAGATGTCCCGGGTCTCCAGCGTTATACCCAGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

286

Amino Acids

30.74

Weight (kDa)

9.67

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 165 3.9e-10 NmrA-like family
Epimerase PF01370 60 - 167 5.3e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 187 1.5e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 208 4.6e-18 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AccB7I CCANNNNNTGG 1 cut(s) 725
AccII CGCG 1 cut(s) 19
AciI CCGC 2 cut(s) 19, 459
AclWI GGATC 2 cut(s) 19, 788
AcsI RAATTY 1 cut(s) 578
AcuI CTGAAG 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 590, 725
AflIII ACRYGT 2 cut(s) 211, 411
AgsI TTSAA 4 cut(s) 124, 338, 358, 692
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 3 cut(s) 308, 607, 808
AluBI AGCT 5 cut(s) 137, 554, 568, 715, 788
AluI AGCT 5 cut(s) 137, 554, 568, 715, 788
Alw26I GTCTC 4 cut(s) 134, 554, 694, 836
AlwI GGATC 2 cut(s) 19, 788
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 826
AoxI GGCC 3 cut(s) 294, 605, 728
ApeKI GCWGC 4 cut(s) 497, 554, 715, 785
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 294, 728
AsuC2I CCSGG 2 cut(s) 827, 828
AsuNHI GCTAGC 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 826
BauI CACGAG 1 cut(s) 697
BbsI GAAGAC 1 cut(s) 793
BbvI GCAGC 4 cut(s) 484, 541, 702, 797
BceAI ACGGC 1 cut(s) 761
BciT130I CCWGG 3 cut(s) 310, 609, 810
BcnI CCSGG 2 cut(s) 827, 828
BcoDI GTCTC 4 cut(s) 134, 554, 694, 836
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 2 cut(s) 527, 555
BglII AGATCT 1 cut(s) 263
BisI GCNGC 5 cut(s) 459, 498, 555, 716, 786
BlsI GCNGC 5 cut(s) 460, 499, 556, 717, 787
Bme1390I CCNGG 5 cut(s) 310, 609, 810, 827, 828
BmeT110I CYCGRG 1 cut(s) 826
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 294, 728
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 5 cut(s) 310, 609, 810, 827, 828
BmsI GCATC 4 cut(s) 55, 322, 340, 672
BmtI GCTAGC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 2 cut(s) 729, 818
Bpu10I CCTNAGC 2 cut(s) 257, 707
BpuEI CTTGAG 1 cut(s) 698
BpuMI CCSGG 2 cut(s) 827, 828
BsaI GGTCTC 2 cut(s) 694, 836
BsaJI CCNNGG 3 cut(s) 47, 809, 826
Bsc4I CCNNNNNNNGG 2 cut(s) 590, 725
Bse1I ACTGG 1 cut(s) 746
BseBI CCWGG 3 cut(s) 310, 609, 810
BseDI CCNNGG 3 cut(s) 47, 809, 826
BseGI GGATG 2 cut(s) 70, 355
BseLI CCNNNNNNNGG 2 cut(s) 590, 725
BseMII CTCAG 2 cut(s) 129, 698
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 4 cut(s) 150, 606, 663, 732
BseXI GCAGC 4 cut(s) 484, 541, 702, 797
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 296, 607, 730
BsiEI CGRYCG 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 826
BsiSI CCGG 1 cut(s) 827
BslFI GGGAC 3 cut(s) 442, 637, 809
BslI CCNNNNNNNGG 2 cut(s) 590, 725
BsmAI GTCTC 4 cut(s) 134, 554, 694, 836
BsmFI GGGAC 3 cut(s) 442, 637, 809
BsnI GGCC 3 cut(s) 296, 607, 730
Bso31I GGTCTC 2 cut(s) 694, 836
BsoBI CYCGRG 1 cut(s) 826
Bsp143I GATC 4 cut(s) 24, 35, 263, 793
BspACI CCGC 2 cut(s) 19, 459
BspANI GGCC 3 cut(s) 296, 607, 730
BspCNI CTCAG 2 cut(s) 130, 699
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 400
BspMAI CTGCAG 2 cut(s) 531, 559
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 19, 788
BspTNI GGTCTC 2 cut(s) 694, 836
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 3 cut(s) 47, 809, 826
BssMI GATC 4 cut(s) 24, 35, 263, 793
BssSI CACGAG 1 cut(s) 697
BssT1I CCWWGG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 697
Bst2UI CCWGG 3 cut(s) 310, 609, 810
Bst4CI ACNGT 4 cut(s) 10, 145, 304, 634
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 2 cut(s) 253, 713
BstDEI CTNAG 3 cut(s) 138, 257, 707
BstF5I GGATG 2 cut(s) 70, 355
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 4 cut(s) 27, 38, 266, 796
BstMAI GTCTC 4 cut(s) 134, 554, 694, 836
BstMBI GATC 4 cut(s) 24, 35, 263, 793
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 773, 782
BstNI CCWGG 3 cut(s) 310, 609, 810
BstNSI RCATGY 2 cut(s) 215, 415
BstSCI CCNGG 5 cut(s) 308, 607, 808, 825, 826
BstSFI CTRYAG 2 cut(s) 527, 555
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 4 cut(s) 484, 541, 702, 797
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 3 cut(s) 296, 607, 730
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 2 cut(s) 70, 355
BtsIMutI CAGTG 3 cut(s) 68, 300, 753
Cac8I GCNNGC 2 cut(s) 253, 713
CaiI CAGNNNCTG 1 cut(s) 791
Cfr13I GGNCC 2 cut(s) 294, 728
Cfr9I CCCGGG 1 cut(s) 826
CviAII CATG 3 cut(s) 212, 284, 412
DdeI CTNAG 3 cut(s) 138, 257, 707
DpnI GATC 4 cut(s) 26, 37, 265, 795
DpnII GATC 4 cut(s) 24, 35, 263, 793
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 607
Eco31I GGTCTC 2 cut(s) 694, 836
Eco57I CTGAAG 1 cut(s) 92
Eco88I CYCGRG 1 cut(s) 826
EcoRII CCWGG 3 cut(s) 308, 607, 808
EcoT14I CCWWGG 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 3 cut(s) 215, 287, 415
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 3 cut(s) 442, 637, 809
FatI CATG 3 cut(s) 211, 283, 411
FauNDI CATATG 1 cut(s) 586
Fnu4HI GCNGC 5 cut(s) 459, 498, 555, 716, 786
FokI GGATG 2 cut(s) 77, 362
Fsp4HI GCNGC 5 cut(s) 459, 498, 555, 716, 786
FspBI CTAG 1 cut(s) 252
GluI GCNGC 5 cut(s) 459, 498, 555, 716, 786
GsuI CTGGAG 2 cut(s) 729, 818
HaeIII GGCC 3 cut(s) 296, 607, 730
HapII CCGG 1 cut(s) 827
Hin1II CATG 3 cut(s) 215, 287, 415
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 4 cut(s) 98, 287, 338, 625
HpaII CCGG 1 cut(s) 827
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 1 cut(s) 677
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 2 cut(s) 130, 335
HpyCH4III ACNGT 4 cut(s) 10, 145, 304, 634
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 4 cut(s) 68, 446, 529, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 773, 782
HpyF3I CTNAG 3 cut(s) 138, 257, 707
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 3 cut(s) 215, 287, 415
Kzo9I GATC 4 cut(s) 24, 35, 263, 793
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 4 cut(s) 484, 541, 702, 797
LweI GCATC 4 cut(s) 55, 322, 340, 672
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 3 cut(s) 304, 364, 754
MalI GATC 4 cut(s) 26, 37, 265, 795
MboI GATC 4 cut(s) 24, 35, 263, 793
MboII GAAGA 3 cut(s) 85, 658, 793
MflI RGATCY 1 cut(s) 263
MluCI AATT 6 cut(s) 278, 403, 447, 517, 578, 663
MlyI GAGTC 2 cut(s) 92, 619
MmeI TCCRAC 2 cut(s) 52, 188
MseI TTAA 2 cut(s) 344, 423
MspA1I CMGCKG 2 cut(s) 461, 715
MspI CCGG 1 cut(s) 827
MspR9I CCNGG 5 cut(s) 310, 609, 810, 827, 828
MvaI CCWGG 3 cut(s) 310, 609, 810
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 2 cut(s) 773, 782
NciI CCSGG 2 cut(s) 827, 828
NdeI CATATG 1 cut(s) 586
NdeII GATC 4 cut(s) 24, 35, 263, 793
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 3 cut(s) 215, 287, 415
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 304, 364
NspI RCATGY 2 cut(s) 215, 415
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 2 cut(s) 211, 411
PfeI GAWTC 2 cut(s) 287, 338
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 5 cut(s) 460, 499, 556, 717, 787
PleI GAGTC 2 cut(s) 92, 619
PpsI GAGTC 2 cut(s) 92, 619
PscI ACATGT 2 cut(s) 211, 411
PshBI ATTAAT 1 cut(s) 423
Psp6I CCWGG 3 cut(s) 308, 607, 808
PspGI CCWGG 3 cut(s) 308, 607, 808
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 2 cut(s) 294, 728
PstI CTGCAG 2 cut(s) 531, 559
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 715
SaqAI TTAA 2 cut(s) 344, 423
SatI GCNGC 5 cut(s) 459, 498, 555, 716, 786
Sau3AI GATC 4 cut(s) 24, 35, 263, 793
Sau96I GGNCC 2 cut(s) 294, 728
SchI GAGTC 2 cut(s) 92, 619
ScrFI CCNGG 5 cut(s) 310, 609, 810, 827, 828
SfaNI GCATC 4 cut(s) 55, 322, 340, 672
SfcI CTRYAG 2 cut(s) 527, 555
SmaI CCCGGG 1 cut(s) 828
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 6 cut(s) 278, 403, 447, 517, 578, 663
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 2 cut(s) 19, 459
SspMI CTAG 1 cut(s) 252
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 5 cut(s) 308, 607, 808, 825, 826
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 4 cut(s) 10, 145, 304, 634
TaiI ACGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 628
TasI AATT 6 cut(s) 278, 403, 447, 517, 578, 663
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 2 cut(s) 287, 338
Tru1I TTAA 2 cut(s) 344, 423
Tru9I TTAA 2 cut(s) 344, 423
TscAI CASTG 3 cut(s) 75, 307, 753
TseFI GTSAC 2 cut(s) 304, 364
TseI GCWGC 4 cut(s) 497, 554, 715, 785
Tsp45I GTSAC 2 cut(s) 304, 364
TspDTI ATGAA 1 cut(s) 129
TspGWI ACGGA 1 cut(s) 361
TspMI CCCGGG 1 cut(s) 826
TspRI CASTG 3 cut(s) 75, 307, 753
Van91I CCANNNNNTGG 1 cut(s) 725
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 1 cut(s) 578
XceI RCATGY 2 cut(s) 215, 415
XmaI CCCGGG 1 cut(s) 826
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.