Rroxscaffold_7G00169060

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012254
Physical Location & Seq
Reverse (-)
9756164 .. 9760095
3932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00169060.1

Sequence Viewer

Length: 723 bp
ATGAGCCCTTCAAAGTTGAGGAAGCAGAGACTATTCAAGCCCCCCTCCTCCAACAGAGAAGGTATAACCCTTTCCCCTCCATTTGATGCTGAAGAATTATTCACTAGCATTGGAAAGTTTAGGTCTGGTAGGTCAAAGTTACATGATTCATGGGCTAACAATGTGACCTGGTACCAAGGAAACCTTCTATCACCTGATTCATGGAAGGATGCTCTTGACGGTGTCACGTCTGTTATATCCTGCGTTGGTGGCTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCAAACATCAATGCAATTAGAGCTGCTTCTGAGCATGGTGTGAAAAGATTTGTGTATGTCTCTGCTGCTGATTTTGGCTTGGTAAATTACTTGCTGCGAGGATATTATGAGGGAAAGAGAGCTGCCGAAACAGAGATACTAACCAAATTCCCTTATGGAGGAGTGATTTTGAGGCCTGGATTTATTTACGGGACTCGAAGTGTTGGGAGCATAAAGTTACCTCTTGGTGTAATCGGTTCTCCTCTGGAGATGTTATTTCAACAAACAAGACCTCTGAACCAGCTGCCACTAGTGGGACCTCTCTTTACTCCTCCTGTGAATGTTACTGCTGTAGCGAAGGTTGCTGTTAGAGCAGCTACCGATCCCGTCTTCCCTCCTGGGATTGTTGATATCTACGGGATTCAACGTTATACTCAGCATAAGTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

240

Amino Acids

26.32

Weight (kDa)

9.83

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 45 - 159 2.8e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 171
AccB1I GGYRCC 1 cut(s) 171
AclI AACGTT 1 cut(s) 697
AclWI GGATC 1 cut(s) 647
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 449, 584
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 4 cut(s) 12, 37, 551, 695
AhlI ACTAGT 1 cut(s) 580
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 3 cut(s) 167, 466, 667
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 4 cut(s) 314, 413, 574, 647
AluI AGCT 4 cut(s) 314, 413, 574, 647
Alw26I GTCTC 2 cut(s) 22, 355
AlwI GGATC 1 cut(s) 647
AoxI GGCC 1 cut(s) 464
ApeKI GCWGC 6 cut(s) 314, 356, 385, 413, 574, 644
ApoI RAATTY 1 cut(s) 437
AseI ATTAAT 1 cut(s) 282
Asp718I GGTACC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 183
AvaII GGWCC 1 cut(s) 587
BanI GGYRCC 1 cut(s) 171
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 652
BbvI GCAGC 6 cut(s) 301, 343, 372, 400, 561, 656
BciT130I CCWGG 3 cut(s) 169, 468, 669
BcoDI GTCTC 2 cut(s) 22, 355
BcuI ACTAGT 1 cut(s) 580
BfaI CTAG 2 cut(s) 105, 581
BfmI CTRYAG 1 cut(s) 621
BisI GCNGC 6 cut(s) 315, 357, 386, 414, 575, 645
BlsI GCNGC 6 cut(s) 316, 358, 387, 415, 576, 646
Bme1390I CCNGG 3 cut(s) 169, 468, 669
Bme18I GGWCC 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 3 cut(s) 173, 259, 588
BmrFI CCNGG 3 cut(s) 169, 468, 669
BmsI GCATC 2 cut(s) 76, 199
BpiI GAAGAC 1 cut(s) 652
BpmI CTGGAG 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 175, 668
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 584
BseBI CCWGG 3 cut(s) 169, 468, 669
BseDI CCNNGG 2 cut(s) 175, 668
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 449, 584
BseMII CTCAG 2 cut(s) 312, 719
BseRI GAGGAG 4 cut(s) 37, 465, 522, 591
BseXI GCAGC 6 cut(s) 301, 343, 372, 400, 561, 656
BshFI GGCC 1 cut(s) 466
BshNI GGYRCC 1 cut(s) 171
BslFI GGGAC 3 cut(s) 301, 496, 600
BslI CCNNNNNNNGG 2 cut(s) 449, 584
BsmAI GTCTC 2 cut(s) 22, 355
BsmFI GGGAC 3 cut(s) 301, 496, 600
BsnI GGCC 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 652
BspANI GGCC 1 cut(s) 466
BspCNI CTCAG 2 cut(s) 313, 718
BspLI GGNNCC 3 cut(s) 173, 259, 588
BspPI GGATC 1 cut(s) 647
BspT107I GGYRCC 1 cut(s) 171
BssECI CCNNGG 2 cut(s) 175, 668
BssMI GATC 1 cut(s) 652
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 3 cut(s) 169, 468, 669
Bst4CI ACNGT 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 321, 705
BstENI CCTNNNNNAGG 1 cut(s) 447
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 1 cut(s) 655
BstMAI GTCTC 2 cut(s) 22, 355
BstMBI GATC 1 cut(s) 652
BstMWI GCNNNNNNNGC 4 cut(s) 249, 311, 632, 641
BstNI CCWGG 3 cut(s) 169, 468, 669
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 3 cut(s) 167, 466, 667
BstSFI CTRYAG 1 cut(s) 621
BstV1I GCAGC 6 cut(s) 301, 343, 372, 400, 561, 656
BstV2I GAAGAC 1 cut(s) 652
BsuRI GGCC 1 cut(s) 466
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 587
CsiI ACCWGGT 1 cut(s) 167
Csp6I GTAC 1 cut(s) 172
CviAII CATG 5 cut(s) 143, 150, 201, 271, 326
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 2 cut(s) 321, 705
DpnI GATC 1 cut(s) 654
DpnII GATC 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 175
Eco147I AGGCCT 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 8
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 587
EcoRII CCWGG 3 cut(s) 167, 466, 667
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 5 cut(s) 146, 153, 204, 274, 329
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FaqI GGGAC 3 cut(s) 301, 496, 600
FatI CATG 5 cut(s) 142, 149, 200, 270, 325
Fnu4HI GCNGC 6 cut(s) 315, 357, 386, 414, 575, 645
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 6 cut(s) 315, 357, 386, 414, 575, 645
FspBI CTAG 2 cut(s) 105, 581
GluI GCNGC 6 cut(s) 315, 357, 386, 414, 575, 645
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 1 cut(s) 466
Hin1II CATG 5 cut(s) 146, 153, 204, 274, 329
HinfI GANTC 4 cut(s) 146, 197, 484, 691
HphI GGTGA 1 cut(s) 183
Hpy188I TCNGA 2 cut(s) 322, 567
Hpy188III TCNNGA 2 cut(s) 215, 536
HpyAV CCTTC 5 cut(s) 18, 53, 194, 199, 622
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 2 cut(s) 227, 697
HpyCH4V TGCA 2 cut(s) 293, 305
HpyF10VI GCNNNNNNNGC 4 cut(s) 249, 311, 632, 641
HpyF3I CTNAG 2 cut(s) 321, 705
HpySE526I ACGT 2 cut(s) 227, 697
Hsp92II CATG 5 cut(s) 146, 153, 204, 274, 329
KpnI GGTACC 1 cut(s) 175
Kzo9I GATC 1 cut(s) 652
LmnI GCTCC 2 cut(s) 263, 498
Lsp1109I GCAGC 6 cut(s) 301, 343, 372, 400, 561, 656
LweI GCATC 2 cut(s) 76, 199
MabI ACCWGGT 1 cut(s) 167
MaeI CTAG 2 cut(s) 105, 581
MaeII ACGT 2 cut(s) 227, 697
MaeIII GTNAC 5 cut(s) 138, 163, 223, 507, 613
MalI GATC 1 cut(s) 654
MboI GATC 1 cut(s) 652
MboII GAAGA 2 cut(s) 104, 652
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 5 cut(s) 95, 262, 306, 376, 437
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 1 cut(s) 75
MseI TTAA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 574
MspR9I CCNGG 3 cut(s) 169, 468, 669
MvaI CCWGG 3 cut(s) 169, 468, 669
MwoI GCNNNNNNNGC 4 cut(s) 249, 311, 632, 641
NdeII GATC 1 cut(s) 652
NlaIII CATG 5 cut(s) 146, 153, 204, 274, 329
NlaIV GGNNCC 3 cut(s) 173, 259, 588
NmuCI GTSAC 2 cut(s) 163, 223
NspI RCATGY 1 cut(s) 274
PceI AGGCCT 1 cut(s) 466
PciI ACATGT 1 cut(s) 270
PfeI GAWTC 3 cut(s) 146, 197, 691
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 6 cut(s) 316, 358, 387, 415, 576, 646
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PpuMI RGGWCCY 1 cut(s) 587
PscI ACATGT 1 cut(s) 270
PshBI ATTAAT 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 697
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 3 cut(s) 167, 466, 667
PspGI CCWGG 3 cut(s) 167, 466, 667
PspN4I GGNNCC 3 cut(s) 173, 259, 588
PspPI GGNCC 1 cut(s) 587
PspPPI RGGWCCY 1 cut(s) 587
PsyI GACNNNGTC 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 574
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 6 cut(s) 315, 357, 386, 414, 575, 645
Sau3AI GATC 1 cut(s) 652
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 3 cut(s) 169, 468, 669
SduI GDGCHC 1 cut(s) 8
SexAI ACCWGGT 1 cut(s) 167
SfaNI GCATC 2 cut(s) 76, 199
SfcI CTRYAG 1 cut(s) 621
SinI GGWCC 1 cut(s) 587
SpeI ACTAGT 1 cut(s) 580
Sse9I AATT 5 cut(s) 95, 262, 306, 376, 437
SseBI AGGCCT 1 cut(s) 466
SspMI CTAG 2 cut(s) 105, 581
StuI AGGCCT 1 cut(s) 466
StyD4I CCNGG 3 cut(s) 167, 466, 667
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 2 cut(s) 230, 700
TaqI TCGA 1 cut(s) 487
TasI AATT 5 cut(s) 95, 262, 306, 376, 437
TfiI GAWTC 3 cut(s) 146, 197, 691
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseFI GTSAC 2 cut(s) 163, 223
TseI GCWGC 6 cut(s) 314, 356, 385, 413, 574, 644
Tsp45I GTSAC 2 cut(s) 163, 223
TspDTI ATGAA 2 cut(s) 138, 189
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 587
VspI ATTAAT 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 447
XapI RAATTY 1 cut(s) 437
XceI RCATGY 1 cut(s) 274
XspI CTAG 2 cut(s) 105, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.