Rw6G034640

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012254
Physical Location & Seq
Forward (+)
59331013 .. 59335118
4106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034640.1

Sequence Viewer

Length: 864 bp
ATGAGGACGGTCGTGTCGCGGTTGATCCAATCAAGATCATCACTTCCCAGGCCCTTTATGATGACTGCACCTATAAATGGGAGGTATCTCTCAGGCGAATCTAATAAGGTTGATGAGCCCTTCCAAGTTGAGGAAGCAGAGACTATTCAAGCCCCCCCTCCTCCAACAGAGAAGTTGCTTGTGTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCATTAGATCGTGGACTGACTGTTGCTAGCCTTAGCAGGTCTGGTAGGTCAAAGTTACATGATTCATGGGCTAACAATGTGAACTGGTACCAAGGAAACCTTCTATCACCTGATTCATGGAAGGATGCTCTTGACGGTGTCACGTCTGTTATATCCTGCGTTGGTGGCTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCAAACATCAATGCAATTAGAGCTGCTTCTGAGCATGGTGTGAAAAGATTTGTGTATGTCTCTGCTGCTGATTTTGGCTTGGTAAATTACTTGCTGCGAGGATATTATGAGGGAAAGAGAGCTGCCGAAACAGAGATACTAACCAAATTCCCTTATGGAGGAGTGATTTTGAGGCCTGGATTTATTTACGGGACTCGAAGTGTTGGGAGCATTAAGTTACCTCTTGGTGTAATCGGTTCTCCTCTGGAGATGTTATTTCAACAAACAAGACCTCTGAACCAGCTGCCACTAGTGGGACCTCTCTTTACTCCTCCTGTGAATGTTACTGCTGTAGCGAAGGTTGCTGTTAGAGCAGCTACCGATCCCGTCTTCCCTCCTGGGATTGTTGATATCTACGGGATTCAACGTTATACTCAGCATAAGTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

287

Amino Acids

31.23

Weight (kDa)

9.56

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 166 6e-11 NmrA-like family
Epimerase PF01370 60 - 167 1.6e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 169 2e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 206 4e-20 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 252
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccII CGCG 1 cut(s) 19
AciI CCGC 1 cut(s) 19
AclI AACGTT 1 cut(s) 838
AclWI GGATC 2 cut(s) 19, 788
AcsI RAATTY 1 cut(s) 578
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 4 cut(s) 77, 130, 590, 725
AflIII ACRYGT 2 cut(s) 211, 411
AgsI TTSAA 3 cut(s) 149, 692, 836
AhlI ACTAGT 1 cut(s) 721
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 3 cut(s) 47, 607, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AluBI AGCT 4 cut(s) 455, 554, 715, 788
AluI AGCT 4 cut(s) 455, 554, 715, 788
Alw26I GTCTC 2 cut(s) 134, 496
AlwI GGATC 2 cut(s) 19, 788
AoxI GGCC 2 cut(s) 50, 605
ApeKI GCWGC 6 cut(s) 455, 497, 526, 554, 715, 785
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 423
Asp718I GGTACC 1 cut(s) 312
AspS9I GGNCC 2 cut(s) 51, 728
AsuHPI GGTGA 1 cut(s) 324
AsuNHI GCTAGC 1 cut(s) 251
AvaII GGWCC 1 cut(s) 728
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 793
BbvI GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
BciT130I CCWGG 3 cut(s) 49, 609, 810
BcoDI GTCTC 2 cut(s) 134, 496
BcuI ACTAGT 1 cut(s) 721
BfaI CTAG 2 cut(s) 252, 722
BfmI CTRYAG 1 cut(s) 762
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
BlsI GCNGC 6 cut(s) 457, 499, 528, 556, 717, 787
Bme1390I CCNGG 3 cut(s) 49, 609, 810
Bme18I GGWCC 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 51, 728
BmiI GGNNCC 3 cut(s) 314, 400, 729
BmrFI CCNGG 3 cut(s) 49, 609, 810
BmsI GCATC 1 cut(s) 340
BmtI GCTAGC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 1 cut(s) 698
Bpu10I CCTNAGC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 47, 316, 809
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 130, 590, 725
Bse1I ACTGG 1 cut(s) 314
BseBI CCWGG 3 cut(s) 49, 609, 810
BseDI CCNNGG 3 cut(s) 47, 316, 809
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 4 cut(s) 77, 130, 590, 725
BseMII CTCAG 3 cut(s) 105, 453, 860
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 4 cut(s) 150, 606, 663, 732
BseXI GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 52, 607
BshNI GGYRCC 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 12
BslFI GGGAC 3 cut(s) 442, 637, 741
BslI CCNNNNNNNGG 4 cut(s) 77, 130, 590, 725
BsmAI GTCTC 2 cut(s) 134, 496
BsmFI GGGAC 3 cut(s) 442, 637, 741
BsnI GGCC 2 cut(s) 52, 607
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 4 cut(s) 24, 35, 232, 793
BspACI CCGC 1 cut(s) 19
BspANI GGCC 2 cut(s) 52, 607
BspCNI CTCAG 3 cut(s) 104, 454, 859
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 3 cut(s) 314, 400, 729
BspMI ACCTGC 1 cut(s) 252
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 19, 788
BspT107I GGYRCC 1 cut(s) 312
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 47, 316, 809
BssMI GATC 4 cut(s) 24, 35, 232, 793
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 3 cut(s) 49, 609, 810
Bst4CI ACNGT 3 cut(s) 10, 247, 362
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 91, 257, 462, 846
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 4 cut(s) 27, 38, 235, 796
BstMAI GTCTC 2 cut(s) 134, 496
BstMBI GATC 4 cut(s) 24, 35, 232, 793
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 4 cut(s) 390, 452, 773, 782
BstNI CCWGG 3 cut(s) 49, 609, 810
BstNSI RCATGY 2 cut(s) 215, 415
BstSCI CCNGG 3 cut(s) 47, 607, 808
BstSFI CTRYAG 1 cut(s) 762
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
BstV2I GAAGAC 1 cut(s) 793
BsuRI GGCC 2 cut(s) 52, 607
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 355
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 51, 728
Csp6I GTAC 1 cut(s) 313
CviAII CATG 6 cut(s) 212, 284, 291, 342, 412, 467
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 4 cut(s) 91, 257, 462, 846
DpnI GATC 4 cut(s) 26, 37, 234, 795
DpnII GATC 4 cut(s) 24, 35, 232, 793
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 316
Eco147I AGGCCT 1 cut(s) 607
Eco24I GRGCYC 1 cut(s) 120
Eco32I GATATC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 728
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoO109I RGGNCCY 2 cut(s) 51, 728
EcoRII CCWGG 3 cut(s) 47, 607, 808
EcoRV GATATC 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 6 cut(s) 215, 287, 294, 345, 415, 470
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 3 cut(s) 442, 637, 741
FatI CATG 6 cut(s) 211, 283, 290, 341, 411, 466
Fnu4HI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
FspBI CTAG 2 cut(s) 252, 722
GluI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 2 cut(s) 52, 607
Hin1II CATG 6 cut(s) 215, 287, 294, 345, 415, 470
HinfI GANTC 5 cut(s) 98, 287, 338, 625, 832
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 2 cut(s) 239, 307
Hpy188I TCNGA 2 cut(s) 463, 708
Hpy188III TCNNGA 3 cut(s) 33, 356, 677
Hpy8I GTNNAC 2 cut(s) 239, 307
HpyAV CCTTC 4 cut(s) 130, 335, 340, 763
HpyCH4III ACNGT 3 cut(s) 10, 247, 362
HpyCH4IV ACGT 2 cut(s) 368, 838
HpyCH4V TGCA 3 cut(s) 68, 434, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 390, 452, 773, 782
HpyF3I CTNAG 4 cut(s) 91, 257, 462, 846
HpySE526I ACGT 2 cut(s) 368, 838
Hsp92II CATG 6 cut(s) 215, 287, 294, 345, 415, 470
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 4 cut(s) 24, 35, 232, 793
LmnI GCTCC 2 cut(s) 404, 639
Lsp1109I GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
LweI GCATC 1 cut(s) 340
MaeI CTAG 2 cut(s) 252, 722
MaeII ACGT 2 cut(s) 368, 838
MaeIII GTNAC 4 cut(s) 279, 364, 648, 754
MalI GATC 4 cut(s) 26, 37, 234, 795
MboI GATC 4 cut(s) 24, 35, 232, 793
MboII GAAGA 1 cut(s) 793
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 4 cut(s) 403, 447, 517, 578
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 1 cut(s) 188
MseI TTAA 2 cut(s) 423, 645
MspA1I CMGCKG 1 cut(s) 715
MspR9I CCNGG 3 cut(s) 49, 609, 810
MvaI CCWGG 3 cut(s) 49, 609, 810
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 4 cut(s) 390, 452, 773, 782
NdeII GATC 4 cut(s) 24, 35, 232, 793
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 6 cut(s) 215, 287, 294, 345, 415, 470
NlaIV GGNNCC 3 cut(s) 314, 400, 729
NmuCI GTSAC 1 cut(s) 364
NspI RCATGY 2 cut(s) 215, 415
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 2 cut(s) 211, 411
PfeI GAWTC 4 cut(s) 98, 287, 338, 832
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 6 cut(s) 457, 499, 528, 556, 717, 787
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PpuMI RGGWCCY 1 cut(s) 728
PscI ACATGT 2 cut(s) 211, 411
PshBI ATTAAT 1 cut(s) 423
Psp1406I AACGTT 1 cut(s) 838
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 3 cut(s) 47, 607, 808
PspGI CCWGG 3 cut(s) 47, 607, 808
PspN4I GGNNCC 3 cut(s) 314, 400, 729
PspPI GGNCC 2 cut(s) 51, 728
PspPPI RGGWCCY 1 cut(s) 728
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 715
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SaqAI TTAA 2 cut(s) 423, 645
SatI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
Sau3AI GATC 4 cut(s) 24, 35, 232, 793
Sau96I GGNCC 2 cut(s) 51, 728
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 3 cut(s) 49, 609, 810
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 728
SpeI ACTAGT 1 cut(s) 721
Sse9I AATT 4 cut(s) 403, 447, 517, 578
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 1 cut(s) 19
SspMI CTAG 2 cut(s) 252, 722
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 3 cut(s) 47, 607, 808
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 3 cut(s) 10, 247, 362
TaiI ACGT 2 cut(s) 371, 841
TaqI TCGA 1 cut(s) 628
TasI AATT 4 cut(s) 403, 447, 517, 578
TfiI GAWTC 4 cut(s) 98, 287, 338, 832
Tru1I TTAA 2 cut(s) 423, 645
Tru9I TTAA 2 cut(s) 423, 645
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 6 cut(s) 455, 497, 526, 554, 715, 785
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 2 cut(s) 279, 330
Tth111I GACNNNGTC 1 cut(s) 362
VpaK11BI GGWCC 1 cut(s) 728
VspI ATTAAT 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 1 cut(s) 578
XceI RCATGY 2 cut(s) 215, 415
XspI CTAG 2 cut(s) 252, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.