MD08G1049300.v1.1

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012254
Physical Location & Seq
Reverse (-)
3843550 .. 3847004
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1049300.v1.1.491

Sequence Viewer

Length: 858 bp
ATGAGGACGGTCGTCTGGCGGTTGATCCATACAAGATCATCACTTCCCAGGCTCTTTGTATCGGATGCACCGAAGAATGGTAGGTATCTGACAACAGACTCTGATAAGGTTGATGAACCCTTCAAAGTTGAGGAAGCAGAGACTGTAAACACTCCCCCTCCGCCAACTGAGAAGTTGCTTGTCTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCTTTGGAGCGGGGCTTGTCTGTCACTAGCCTTAGCAGGTCTGGTAGGTCAAAATTACATGATTCATGGGCCGACAGTATGACCTGGCATCAAGGAAACCTTCTATCGCCTGAGTCATGGAAGGATGCCCTCGATGGTGTCACGTCTGTGATATCCTGTGTTGGTGGGTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCTAACATCAATGCAATTAGAGCTGCCGCTGAACAAGGTGTGAAAAGATTTGTTTATATCTCTGCTGCTGATTTTGGCTTGGCAAATTACTTATTGCGGGGATATTATGAGGGAAAGAGAGCTGCAGAGACAGAGCTACTAACCAAATTCCCTTACGGAGGAGTGATTTTGAGGCCTGGATTTATTTATGGGACTCGAAGCGTTGGCAGCGTAAAGTTACCTCTCGGTGTAATTGGTTATCCACTTGAGATGCTATTTCAATATACAAGACCTCTGAGCCAGCTTCCGCTTGTTGGGCCTCTCTTTACTCCTCCAGTGAATGTCACCTCCGTGGCAAATGTTGCCGTTACAGCAGCTACTGATCCCGTCTTCCCTCCTGGGATCGTAGATGTCCAAGGGATCCAGCGTTATACCCAGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

286

Amino Acids

31.08

Weight (kDa)

9.24

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 165 5.1e-10 NmrA-like family
Epimerase PF01370 60 - 167 2.3e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 171 8.9e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 207 8.4e-20 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccBSI CCGCTC 1 cut(s) 235
AciI CCGC 6 cut(s) 19, 161, 235, 459, 529, 719
AclWI GGATC 5 cut(s) 19, 788, 821, 826, 839
AcsI RAATTY 1 cut(s) 578
AfiI CCNNNNNNNGG 3 cut(s) 77, 590, 725
AflIII ACRYGT 2 cut(s) 211, 411
AgsI TTSAA 2 cut(s) 124, 692
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 4 cut(s) 47, 308, 607, 808
AleI CACNNNNGTG 2 cut(s) 371, 761
AluBI AGCT 6 cut(s) 227, 455, 554, 568, 715, 788
AluI AGCT 6 cut(s) 227, 455, 554, 568, 715, 788
Alw26I GTCTC 2 cut(s) 134, 554
AlwI GGATC 5 cut(s) 19, 788, 821, 826, 839
AlwNI CAGNNNCTG 3 cut(s) 101, 143, 791
AoxI GGCC 3 cut(s) 294, 605, 728
ApeKI GCWGC 5 cut(s) 455, 497, 554, 639, 785
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 294, 728
AsuHPI GGTGA 1 cut(s) 748
BamHI GGATCC 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 793
BbvI GCAGC 5 cut(s) 442, 484, 541, 651, 797
BccI CCATC 1 cut(s) 353
BceAI ACGGC 1 cut(s) 761
BciT130I CCWGG 4 cut(s) 49, 310, 609, 810
BcoDI GTCTC 2 cut(s) 134, 554
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 555
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 6 cut(s) 456, 459, 498, 555, 640, 786
BlsI GCNGC 6 cut(s) 457, 460, 499, 556, 641, 787
Bme1390I CCNGG 4 cut(s) 49, 310, 609, 810
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 294, 728
BmiI GGNNCC 2 cut(s) 400, 833
BmrFI CCNGG 4 cut(s) 49, 310, 609, 810
BmsI GCATC 4 cut(s) 55, 322, 340, 672
BoxI GACNNNNGTC 1 cut(s) 11
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 1 cut(s) 729
Bpu10I CCTNAGC 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 698
BsaJI CCNNGG 4 cut(s) 47, 762, 809, 826
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 590, 725
Bse1I ACTGG 1 cut(s) 746
BseBI CCWGG 4 cut(s) 49, 310, 609, 810
BseDI CCNNGG 4 cut(s) 47, 762, 809, 826
BseGI GGATG 2 cut(s) 70, 355
BseLI CCNNNNNNNGG 3 cut(s) 77, 590, 725
BseMII CTCAG 3 cut(s) 159, 327, 698
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 2 cut(s) 606, 732
BseXI GCAGC 5 cut(s) 442, 484, 541, 651, 797
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 296, 607, 730
BsiEI CGRYCG 1 cut(s) 12
BslFI GGGAC 2 cut(s) 442, 637
BslI CCNNNNNNNGG 3 cut(s) 77, 590, 725
BsmAI GTCTC 2 cut(s) 134, 554
BsmFI GGGAC 2 cut(s) 442, 637
BsnI GGCC 3 cut(s) 296, 607, 730
Bsp143I GATC 5 cut(s) 24, 35, 793, 813, 831
BspACI CCGC 6 cut(s) 19, 161, 235, 459, 529, 719
BspANI GGCC 3 cut(s) 296, 607, 730
BspCNI CTCAG 3 cut(s) 160, 328, 699
BspLI GGNNCC 2 cut(s) 400, 833
BspMAI CTGCAG 1 cut(s) 559
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 5 cut(s) 19, 788, 821, 826, 839
BsrBI CCGCTC 1 cut(s) 235
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 4 cut(s) 47, 762, 809, 826
BssMI GATC 5 cut(s) 24, 35, 793, 813, 831
BssT1I CCWWGG 1 cut(s) 826
Bst2UI CCWGG 4 cut(s) 49, 310, 609, 810
Bst4CI ACNGT 3 cut(s) 10, 145, 302
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 1 cut(s) 713
BstDEI CTNAG 4 cut(s) 168, 257, 336, 707
BstDSI CCRYGG 1 cut(s) 762
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 2 cut(s) 70, 355
BstKTI GATC 5 cut(s) 27, 38, 796, 816, 834
BstMAI GTCTC 2 cut(s) 134, 554
BstMBI GATC 5 cut(s) 24, 35, 793, 813, 831
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 5 cut(s) 452, 639, 727, 773, 782
BstNI CCWGG 4 cut(s) 49, 310, 609, 810
BstNSI RCATGY 2 cut(s) 215, 415
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 4 cut(s) 47, 308, 607, 808
BstSFI CTRYAG 1 cut(s) 555
BstV1I GCAGC 5 cut(s) 442, 484, 541, 651, 797
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 831
BstYI RGATCY 1 cut(s) 831
BsuRI GGCC 3 cut(s) 296, 607, 730
BtgI CCRYGG 1 cut(s) 762
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 2 cut(s) 70, 355
BtsIMutI CAGTG 1 cut(s) 753
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 713
CaiI CAGNNNCTG 3 cut(s) 101, 143, 791
Cfr13I GGNCC 2 cut(s) 294, 728
CviAII CATG 5 cut(s) 212, 284, 291, 342, 412
DdeI CTNAG 4 cut(s) 168, 257, 336, 707
DpnI GATC 5 cut(s) 26, 37, 795, 815, 833
DpnII GATC 5 cut(s) 24, 35, 793, 813, 831
EciI GGCGGA 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 826
Eco147I AGGCCT 1 cut(s) 607
Eco32I GATATC 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoRII CCWGG 4 cut(s) 47, 308, 607, 808
EcoRV GATATC 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 826
ErhI CCWWGG 1 cut(s) 826
FaeI CATG 5 cut(s) 215, 287, 294, 345, 415
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 2 cut(s) 442, 637
FatI CATG 5 cut(s) 211, 283, 290, 341, 411
FauI CCCGC 2 cut(s) 228, 522
Fnu4HI GCNGC 6 cut(s) 456, 459, 498, 555, 640, 786
FokI GGATG 2 cut(s) 77, 362
Fsp4HI GCNGC 6 cut(s) 456, 459, 498, 555, 640, 786
FspBI CTAG 1 cut(s) 252
GluI GCNGC 6 cut(s) 456, 459, 498, 555, 640, 786
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 3 cut(s) 296, 607, 730
Hin1II CATG 5 cut(s) 215, 287, 294, 345, 415
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 4 cut(s) 98, 287, 338, 625
HphI GGTGA 1 cut(s) 748
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 4 cut(s) 64, 90, 103, 708
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 130, 335, 340
HpyCH4III ACNGT 3 cut(s) 10, 145, 302
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 3 cut(s) 68, 446, 557
HpyF10VI GCNNNNNNNGC 5 cut(s) 452, 639, 727, 773, 782
HpyF3I CTNAG 4 cut(s) 168, 257, 336, 707
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 5 cut(s) 215, 287, 294, 345, 415
Kzo9I GATC 5 cut(s) 24, 35, 793, 813, 831
LmnI GCTCC 2 cut(s) 232, 404
Lsp1109I GCAGC 5 cut(s) 442, 484, 541, 651, 797
LweI GCATC 4 cut(s) 55, 322, 340, 672
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 5 cut(s) 247, 364, 648, 754, 778
MalI GATC 5 cut(s) 26, 37, 795, 815, 833
MbiI CCGCTC 1 cut(s) 235
MboI GATC 5 cut(s) 24, 35, 793, 813, 831
MboII GAAGA 2 cut(s) 85, 793
MflI RGATCY 1 cut(s) 831
MluCI AATT 6 cut(s) 278, 403, 447, 517, 578, 663
MlyI GAGTC 3 cut(s) 92, 347, 619
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 2 cut(s) 371, 761
MspA1I CMGCKG 1 cut(s) 461
MspR9I CCNGG 4 cut(s) 49, 310, 609, 810
MvaI CCWGG 4 cut(s) 49, 310, 609, 810
MwoI GCNNNNNNNGC 5 cut(s) 452, 639, 727, 773, 782
NdeII GATC 5 cut(s) 24, 35, 793, 813, 831
NlaIII CATG 5 cut(s) 215, 287, 294, 345, 415
NlaIV GGNNCC 2 cut(s) 400, 833
NmuCI GTSAC 3 cut(s) 247, 364, 754
NspI RCATGY 2 cut(s) 215, 415
OliI CACNNNNGTG 2 cut(s) 371, 761
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 2 cut(s) 211, 411
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 6 cut(s) 457, 460, 499, 556, 641, 787
PleI GAGTC 3 cut(s) 92, 346, 619
PpsI GAGTC 3 cut(s) 92, 346, 619
PscI ACATGT 2 cut(s) 211, 411
PshAI GACNNNNGTC 1 cut(s) 11
PshBI ATTAAT 1 cut(s) 423
Psp6I CCWGG 4 cut(s) 47, 308, 607, 808
PspGI CCWGG 4 cut(s) 47, 308, 607, 808
PspN4I GGNNCC 2 cut(s) 400, 833
PspPI GGNCC 2 cut(s) 294, 728
PstI CTGCAG 1 cut(s) 559
PstNI CAGNNNCTG 3 cut(s) 101, 143, 791
PsuI RGATCY 1 cut(s) 831
RseI CAYNNNNRTG 2 cut(s) 371, 761
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 6 cut(s) 456, 459, 498, 555, 640, 786
Sau3AI GATC 5 cut(s) 24, 35, 793, 813, 831
Sau96I GGNCC 2 cut(s) 294, 728
SchI GAGTC 3 cut(s) 92, 347, 619
ScrFI CCNGG 4 cut(s) 49, 310, 609, 810
SfaNI GCATC 4 cut(s) 55, 322, 340, 672
SfcI CTRYAG 1 cut(s) 555
SmiMI CAYNNNNRTG 2 cut(s) 371, 761
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 6 cut(s) 278, 403, 447, 517, 578, 663
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 6 cut(s) 19, 161, 235, 459, 529, 719
SspMI CTAG 1 cut(s) 252
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 4 cut(s) 47, 308, 607, 808
StyI CCWWGG 1 cut(s) 826
TaaI ACNGT 3 cut(s) 10, 145, 302
TaiI ACGT 1 cut(s) 371
TaqI TCGA 2 cut(s) 357, 628
TasI AATT 6 cut(s) 278, 403, 447, 517, 578, 663
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 753
TseFI GTSAC 3 cut(s) 247, 364, 754
TseI GCWGC 5 cut(s) 455, 497, 554, 639, 785
Tsp45I GTSAC 3 cut(s) 247, 364, 754
TspDTI ATGAA 2 cut(s) 129, 279
TspGWI ACGGA 2 cut(s) 603, 751
TspRI CASTG 1 cut(s) 753
VspI ATTAAT 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 1 cut(s) 578
XceI RCATGY 2 cut(s) 215, 415
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.