RLG00000011467

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012254
Physical Location & Seq
Reverse (-)
9644639 .. 9648283
3645 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011467

Sequence Viewer

Length: 864 bp
ATGAGGACGGTCGTGTCGCGGTTGATCCATTCAAAATCATCACTTCCCAGGCCCCTTATGATGACTGCACCTATAAATGGGAGGTATCTCTCAGGCGAATCTAATAAGGTTGATGAGCACTTCAAAGTTGAGGAAGCAGAGACTATTCAAGCCCCACCTCTTCCAACAGAGAAGTTACTTGTGTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCATTAGATCGTGGACTGTCTGTTGCTAGCCTTAGCAGGTCTGGTAGGTCAAAGTTACATGATTCATGGGCTAACAATGTGACCTGGTACCAAGGAAACCTTCTATCACCTGATTCATGGAAGGATGCTCTCGACGGTGTCACGTCTGTTATATCCTGTGTTGGTGGCTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCAAACATCAATGCAATTAGAGCTGCTTCTGAGCATGGTGTGAAAAGATTTGTGTATGTCTCTGCTGCTGATTTTGGCTTGGCAAATTACTTGCTGCGAGGATATTATGAGGGAAAGAGAGCTGCCGAAACAGAGGTACTAACCAAATTCCCTTATGGAGGAGTGATTTTGAGGCCTGGATTTATTTACGGGACTCGAAGTGTTTGGAGCATAAAGTTACCTCTTGGTGTAATCGGTTCTCCTCTAGAGATGTTATTTCAACAAACTAGACCTCTGAACCAGCTGCCACTAGTGGGACCTCTCTTTACTCCTCCTGTGAATGTTACTGCTGTAGCGAAGGTTGCTGTTAGAGCAGCTACCGATCCCGTCTTCCCTCCTGGGATTGTTGATATCTACGGGATCCAACGTTATACTCAGCATAAGTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

288

Amino Acids

31.29

Weight (kDa)

9.59

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 166 1.6e-11 NmrA-like family
Epimerase PF01370 60 - 167 2.6e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 170 2.6e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 206 2.2e-20 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 252
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccII CGCG 1 cut(s) 19
AciI CCGC 1 cut(s) 19
AclI AACGTT 1 cut(s) 838
AclWI GGATC 4 cut(s) 19, 788, 826, 839
AcsI RAATTY 1 cut(s) 578
AfaI GTAC 2 cut(s) 314, 570
AfiI CCNNNNNNNGG 3 cut(s) 77, 590, 725
AflIII ACRYGT 2 cut(s) 211, 411
AgsI TTSAA 4 cut(s) 33, 124, 149, 692
AhlI ACTAGT 1 cut(s) 721
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 4 cut(s) 47, 308, 607, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AluBI AGCT 4 cut(s) 455, 554, 715, 788
AluI AGCT 4 cut(s) 455, 554, 715, 788
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 2 cut(s) 134, 496
AlwI GGATC 4 cut(s) 19, 788, 826, 839
AoxI GGCC 2 cut(s) 50, 605
ApeKI GCWGC 6 cut(s) 455, 497, 526, 554, 715, 785
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 423
Asp718I GGTACC 1 cut(s) 312
AspS9I GGNCC 2 cut(s) 51, 728
AsuHPI GGTGA 1 cut(s) 324
AsuNHI GCTAGC 1 cut(s) 251
AvaII GGWCC 1 cut(s) 728
BamHI GGATCC 1 cut(s) 831
BanI GGYRCC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 793
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
BciT130I CCWGG 4 cut(s) 49, 310, 609, 810
BcoDI GTCTC 2 cut(s) 134, 496
BcuI ACTAGT 1 cut(s) 721
BfaI CTAG 4 cut(s) 252, 677, 699, 722
BfmI CTRYAG 1 cut(s) 762
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
BlsI GCNGC 6 cut(s) 457, 499, 528, 556, 717, 787
Bme1390I CCNGG 4 cut(s) 49, 310, 609, 810
Bme18I GGWCC 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 51, 728
BmiI GGNNCC 5 cut(s) 53, 314, 400, 729, 833
BmrFI CCNGG 4 cut(s) 49, 310, 609, 810
BmsI GCATC 1 cut(s) 340
BmtI GCTAGC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 793
Bpu10I CCTNAGC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 47, 316, 809
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 590, 725
BseBI CCWGG 4 cut(s) 49, 310, 609, 810
BseDI CCNNGG 3 cut(s) 47, 316, 809
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 3 cut(s) 77, 590, 725
BseMII CTCAG 3 cut(s) 105, 453, 860
BseRI GAGGAG 3 cut(s) 606, 663, 732
BseXI GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 52, 607
BshNI GGYRCC 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 120
BslFI GGGAC 3 cut(s) 442, 637, 741
BslI CCNNNNNNNGG 3 cut(s) 77, 590, 725
BsmAI GTCTC 2 cut(s) 134, 496
BsmFI GGGAC 3 cut(s) 442, 637, 741
BsnI GGCC 2 cut(s) 52, 607
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 4 cut(s) 24, 232, 793, 831
BspACI CCGC 1 cut(s) 19
BspANI GGCC 2 cut(s) 52, 607
BspCNI CTCAG 3 cut(s) 104, 454, 859
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 5 cut(s) 53, 314, 400, 729, 833
BspMI ACCTGC 1 cut(s) 252
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 4 cut(s) 19, 788, 826, 839
BspT107I GGYRCC 1 cut(s) 312
BssECI CCNNGG 3 cut(s) 47, 316, 809
BssMI GATC 4 cut(s) 24, 232, 793, 831
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 4 cut(s) 49, 310, 609, 810
Bst4CI ACNGT 3 cut(s) 10, 243, 362
Bst6I CTCTTC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 91, 257, 462, 846
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 4 cut(s) 27, 235, 796, 834
BstMAI GTCTC 2 cut(s) 134, 496
BstMBI GATC 4 cut(s) 24, 232, 793, 831
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 452, 773, 782
BstNI CCWGG 4 cut(s) 49, 310, 609, 810
BstNSI RCATGY 2 cut(s) 215, 415
BstSCI CCNGG 4 cut(s) 47, 308, 607, 808
BstSFI CTRYAG 1 cut(s) 762
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 831
BstYI RGATCY 1 cut(s) 831
BsuRI GGCC 2 cut(s) 52, 607
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 355
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 51, 728
CsiI ACCWGGT 1 cut(s) 308
Csp6I GTAC 2 cut(s) 313, 569
CviAII CATG 6 cut(s) 212, 284, 291, 342, 412, 467
CviQI GTAC 2 cut(s) 313, 569
DdeI CTNAG 4 cut(s) 91, 257, 462, 846
DpnI GATC 4 cut(s) 26, 234, 795, 833
DpnII GATC 4 cut(s) 24, 232, 793, 831
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 316
Eco147I AGGCCT 1 cut(s) 607
Eco32I GATATC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 728
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoO109I RGGNCCY 2 cut(s) 51, 728
EcoRII CCWGG 4 cut(s) 47, 308, 607, 808
EcoRV GATATC 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 6 cut(s) 215, 287, 294, 345, 415, 470
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 3 cut(s) 442, 637, 741
FatI CATG 6 cut(s) 211, 283, 290, 341, 411, 466
Fnu4HI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
FokI GGATG 1 cut(s) 362
Fsp4HI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
FspBI CTAG 4 cut(s) 252, 677, 699, 722
GluI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
HaeIII GGCC 2 cut(s) 52, 607
Hin1II CATG 6 cut(s) 215, 287, 294, 345, 415, 470
HinfI GANTC 4 cut(s) 98, 287, 338, 625
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 2 cut(s) 463, 708
Hpy188III TCNNGA 2 cut(s) 356, 677
Hpy8I GTNNAC 1 cut(s) 239
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 3 cut(s) 335, 340, 763
HpyCH4III ACNGT 3 cut(s) 10, 243, 362
HpyCH4IV ACGT 2 cut(s) 368, 838
HpyCH4V TGCA 3 cut(s) 68, 434, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 773, 782
HpyF3I CTNAG 4 cut(s) 91, 257, 462, 846
HpySE526I ACGT 2 cut(s) 368, 838
Hsp92II CATG 6 cut(s) 215, 287, 294, 345, 415, 470
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 4 cut(s) 24, 232, 793, 831
LmnI GCTCC 2 cut(s) 404, 639
Lsp1109I GCAGC 6 cut(s) 442, 484, 513, 541, 702, 797
LweI GCATC 1 cut(s) 340
MabI ACCWGGT 1 cut(s) 308
MaeI CTAG 4 cut(s) 252, 677, 699, 722
MaeII ACGT 2 cut(s) 368, 838
MaeIII GTNAC 6 cut(s) 174, 279, 304, 364, 648, 754
MalI GATC 4 cut(s) 26, 234, 795, 833
MboI GATC 4 cut(s) 24, 232, 793, 831
MboII GAAGA 2 cut(s) 152, 793
MflI RGATCY 1 cut(s) 831
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 4 cut(s) 403, 447, 517, 578
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 2 cut(s) 188, 859
MseI TTAA 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 715
MspR9I CCNGG 4 cut(s) 49, 310, 609, 810
MvaI CCWGG 4 cut(s) 49, 310, 609, 810
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 3 cut(s) 452, 773, 782
NdeII GATC 4 cut(s) 24, 232, 793, 831
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 6 cut(s) 215, 287, 294, 345, 415, 470
NlaIV GGNNCC 5 cut(s) 53, 314, 400, 729, 833
NmuCI GTSAC 2 cut(s) 304, 364
NspI RCATGY 2 cut(s) 215, 415
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 2 cut(s) 211, 411
PfeI GAWTC 3 cut(s) 98, 287, 338
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 6 cut(s) 457, 499, 528, 556, 717, 787
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PpuMI RGGWCCY 1 cut(s) 728
PscI ACATGT 2 cut(s) 211, 411
PshBI ATTAAT 1 cut(s) 423
Psp1406I AACGTT 1 cut(s) 838
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 4 cut(s) 47, 308, 607, 808
PspGI CCWGG 4 cut(s) 47, 308, 607, 808
PspN4I GGNNCC 5 cut(s) 53, 314, 400, 729, 833
PspPI GGNCC 2 cut(s) 51, 728
PspPPI RGGWCCY 1 cut(s) 728
PsuI RGATCY 1 cut(s) 831
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 715
RsaI GTAC 2 cut(s) 314, 570
RsaNI GTAC 2 cut(s) 313, 569
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 6 cut(s) 456, 498, 527, 555, 716, 786
Sau3AI GATC 4 cut(s) 24, 232, 793, 831
Sau96I GGNCC 2 cut(s) 51, 728
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 4 cut(s) 49, 310, 609, 810
SduI GDGCHC 1 cut(s) 120
SexAI ACCWGGT 1 cut(s) 308
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 728
SpeI ACTAGT 1 cut(s) 721
Sse9I AATT 4 cut(s) 403, 447, 517, 578
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 1 cut(s) 19
SspMI CTAG 4 cut(s) 252, 677, 699, 722
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 4 cut(s) 47, 308, 607, 808
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 3 cut(s) 10, 243, 362
TaiI ACGT 2 cut(s) 371, 841
TaqI TCGA 2 cut(s) 357, 628
TasI AATT 4 cut(s) 403, 447, 517, 578
TfiI GAWTC 3 cut(s) 98, 287, 338
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseFI GTSAC 2 cut(s) 304, 364
TseI GCWGC 6 cut(s) 455, 497, 526, 554, 715, 785
Tsp45I GTSAC 2 cut(s) 304, 364
TspDTI ATGAA 2 cut(s) 279, 330
Tth111I GACNNNGTC 1 cut(s) 362
VpaK11BI GGWCC 1 cut(s) 728
VspI ATTAAT 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 1 cut(s) 578
XbaI TCTAGA 1 cut(s) 676
XceI RCATGY 2 cut(s) 215, 415
XspI CTAG 4 cut(s) 252, 677, 699, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.