Prupe.1G399300_v2.0.a1

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012254
Physical Location & Seq
Forward (+)
35304225 .. 35307526
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G399300.1

Sequence Viewer

Length: 843 bp
ATGAGGACGGTCGTGTCGCGGTTGATCCATTCAAGATCATCACTTCCCAGGCTCTTGAATGGGAGGTATCTCTCAACAGACTCTAGTAAGGTGGATGAACCCTTCAAAGTTGAGGAAGCAGAGACTGTAAACATTCCCCCTCCTCCAACAGAGAAGTTGCTTGTGTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAGGCATTGGATCGTGGATTGTCTGTTGCCAGCCTTAGCAGGTCTGGTAGGTCATCATTACATGATCCATGGGCCAGCAATGTGACCTGGCATAAAGGAAACCTTCTATCACCTGAGTCATTGAAGGATGCTCTCGATGGTGTCACGTCTGTTATATCCTGTGTTGGTGGGTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCTAATATAAATGCAATTAGAGCTGCTTCTGAACAAGGTGTGAAACGATTTGTGTATGTCTCTGCTGCTGATTTTGGTGTGGTAAATTACTTACTGCGAGGATATTATGAGGGGAAGAGAGCTGCAGAAACAGAGCTACTAACCAAATTCCCTTATGGAGGAGTGATTTTGAGGCCTGGATTTATTTATGGGACTCGAAGCGTTGGGAGCGTAAAGTTACCTCTCGGAGTTATCGGTTCTCCACTGGAGATGTTGTTCCAACACACAAGACCTCTGAACCAGCTGCCACTTGTTGGGCCTCTCTTTACTCCTCCAGTGAATGTTACAGCAGTGGCAAACGTTGCTGTTAGAGCAGCTACTGATCCCGTCTTCCCTCCTGGGATTGTAGATGTCTATGGAATTCAGCGTTACAACCAGAAGTCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

281

Amino Acids

30.24

Weight (kDa)

9.58

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 707
AccII CGCG 1 cut(s) 19
AciI CCGC 1 cut(s) 19
AclI AACGTT 1 cut(s) 753
AclWI GGATC 4 cut(s) 19, 222, 263, 770
AcsI RAATTY 2 cut(s) 560, 813
AfiI CCNNNNNNNGG 2 cut(s) 572, 707
AflIII ACRYGT 2 cut(s) 193, 393
AgsI TTSAA 4 cut(s) 33, 58, 106, 328
AjiI CACGTC 1 cut(s) 351
AjnI CCWGG 4 cut(s) 47, 290, 589, 790
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 5 cut(s) 437, 536, 550, 697, 770
AluI AGCT 5 cut(s) 437, 536, 550, 697, 770
Alw26I GTCTC 2 cut(s) 116, 478
AlwI GGATC 4 cut(s) 19, 222, 263, 770
AlwNI CAGNNNCTG 2 cut(s) 125, 773
AoxI GGCC 3 cut(s) 276, 587, 710
ApeKI GCWGC 5 cut(s) 437, 479, 536, 697, 767
ApoI RAATTY 2 cut(s) 560, 813
AseI ATTAAT 1 cut(s) 405
AspS9I GGNCC 2 cut(s) 276, 710
AsuHPI GGTGA 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 775
BbvI GCAGC 5 cut(s) 424, 466, 523, 684, 779
BccI CCATC 1 cut(s) 335
BciT130I CCWGG 4 cut(s) 49, 292, 591, 792
BcoDI GTCTC 2 cut(s) 116, 478
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 234
BisI GCNGC 5 cut(s) 438, 480, 537, 698, 768
BlsI GCNGC 5 cut(s) 439, 481, 538, 699, 769
Bme1390I CCNGG 4 cut(s) 49, 292, 591, 792
BmgBI CACGTC 1 cut(s) 351
BmgT120I GGNCC 2 cut(s) 276, 710
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 4 cut(s) 49, 292, 591, 792
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 775
BpmI CTGGAG 2 cut(s) 680, 711
Bpu10I CCTNAGC 1 cut(s) 239
BsaJI CCNNGG 3 cut(s) 47, 272, 791
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 707
Bse1I ACTGG 2 cut(s) 663, 728
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 4 cut(s) 49, 292, 591, 792
BseDI CCNNGG 3 cut(s) 47, 272, 791
BseGI GGATG 2 cut(s) 100, 337
BseLI CCNNNNNNNGG 2 cut(s) 572, 707
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 663, 728
BseRI GAGGAG 3 cut(s) 132, 588, 714
BseXI GCAGC 5 cut(s) 424, 466, 523, 684, 779
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 278, 589, 712
BsiEI CGRYCG 1 cut(s) 12
BslFI GGGAC 2 cut(s) 424, 619
BslI CCNNNNNNNGG 2 cut(s) 572, 707
BsmAI GTCTC 2 cut(s) 116, 478
BsmFI GGGAC 2 cut(s) 424, 619
BsnI GGCC 3 cut(s) 278, 589, 712
Bsp143I GATC 5 cut(s) 24, 35, 214, 268, 775
Bsp19I CCATGG 1 cut(s) 272
BspACI CCGC 1 cut(s) 19
BspANI GGCC 3 cut(s) 278, 589, 712
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 234
BspPI GGATC 4 cut(s) 19, 222, 263, 770
BsrDI GCAATG 1 cut(s) 289
BsrI ACTGG 2 cut(s) 663, 728
BssECI CCNNGG 3 cut(s) 47, 272, 791
BssMI GATC 5 cut(s) 24, 35, 214, 268, 775
BssT1I CCWWGG 1 cut(s) 272
Bst2UI CCWGG 4 cut(s) 49, 292, 591, 792
Bst4CI ACNGT 2 cut(s) 10, 127
Bst6I CTCTTC 1 cut(s) 524
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 2 cut(s) 235, 280
BstDEI CTNAG 2 cut(s) 239, 318
BstDSI CCRYGG 1 cut(s) 272
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 2 cut(s) 100, 337
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 5 cut(s) 27, 38, 217, 271, 778
BstMAI GTCTC 2 cut(s) 116, 478
BstMBI GATC 5 cut(s) 24, 35, 214, 268, 775
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 4 cut(s) 434, 621, 755, 764
BstNI CCWGG 4 cut(s) 49, 292, 591, 792
BstNSI RCATGY 2 cut(s) 197, 397
BstSCI CCNGG 4 cut(s) 47, 290, 589, 790
BstSFI CTRYAG 1 cut(s) 537
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 5 cut(s) 424, 466, 523, 684, 779
BstV2I GAAGAC 1 cut(s) 775
BsuRI GGCC 3 cut(s) 278, 589, 712
BtgI CCRYGG 1 cut(s) 272
BtrI CACGTC 1 cut(s) 351
BtsCI GGATG 2 cut(s) 100, 337
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 3 cut(s) 656, 735, 750
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 235, 280
CaiI CAGNNNCTG 2 cut(s) 125, 773
Cfr13I GGNCC 2 cut(s) 276, 710
CviAII CATG 4 cut(s) 194, 266, 273, 394
DdeI CTNAG 2 cut(s) 239, 318
DpnI GATC 5 cut(s) 26, 37, 216, 270, 777
DpnII GATC 5 cut(s) 24, 35, 214, 268, 775
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 272
Eco147I AGGCCT 1 cut(s) 589
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoRI GAATTC 1 cut(s) 813
EcoRII CCWGG 4 cut(s) 47, 290, 589, 790
EcoT14I CCWWGG 1 cut(s) 272
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 4 cut(s) 197, 269, 276, 397
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FaqI GGGAC 2 cut(s) 424, 619
FatI CATG 4 cut(s) 193, 265, 272, 393
Fnu4HI GCNGC 5 cut(s) 438, 480, 537, 698, 768
FokI GGATG 2 cut(s) 107, 344
Fsp4HI GCNGC 5 cut(s) 438, 480, 537, 698, 768
FspBI CTAG 1 cut(s) 84
GluI GCNGC 5 cut(s) 438, 480, 537, 698, 768
GsuI CTGGAG 2 cut(s) 680, 711
HaeIII GGCC 3 cut(s) 278, 589, 712
Hin1II CATG 4 cut(s) 197, 269, 276, 397
HinfI GANTC 3 cut(s) 80, 320, 607
HphI GGTGA 1 cut(s) 306
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 3 cut(s) 445, 641, 690
Hpy188III TCNNGA 3 cut(s) 33, 55, 338
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 3 cut(s) 112, 317, 322
HpyCH4III ACNGT 2 cut(s) 10, 127
HpyCH4IV ACGT 2 cut(s) 350, 753
HpyCH4V TGCA 2 cut(s) 428, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 621, 755, 764
HpyF3I CTNAG 2 cut(s) 239, 318
HpySE526I ACGT 2 cut(s) 350, 753
Hsp92II CATG 4 cut(s) 197, 269, 276, 397
Kzo9I GATC 5 cut(s) 24, 35, 214, 268, 775
LmnI GCTCC 2 cut(s) 386, 621
Lsp1109I GCAGC 5 cut(s) 424, 466, 523, 684, 779
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 84
MaeII ACGT 2 cut(s) 350, 753
MaeIII GTNAC 5 cut(s) 286, 346, 630, 736, 821
MalI GATC 5 cut(s) 26, 37, 216, 270, 777
MboI GATC 5 cut(s) 24, 35, 214, 268, 775
MboII GAAGA 2 cut(s) 541, 775
MluCI AATT 5 cut(s) 385, 429, 499, 560, 813
MlyI GAGTC 3 cut(s) 74, 329, 601
MmeI TCCRAC 2 cut(s) 170, 697
MseI TTAA 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 697
MspR9I CCNGG 4 cut(s) 49, 292, 591, 792
MvaI CCWGG 4 cut(s) 49, 292, 591, 792
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 4 cut(s) 434, 621, 755, 764
NcoI CCATGG 1 cut(s) 272
NdeII GATC 5 cut(s) 24, 35, 214, 268, 775
NlaIII CATG 4 cut(s) 197, 269, 276, 397
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 2 cut(s) 286, 346
NspI RCATGY 2 cut(s) 197, 397
PceI AGGCCT 1 cut(s) 589
PciI ACATGT 2 cut(s) 193, 393
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 5 cut(s) 439, 481, 538, 699, 769
PleI GAGTC 3 cut(s) 74, 328, 601
PpsI GAGTC 3 cut(s) 74, 328, 601
PscI ACATGT 2 cut(s) 193, 393
PshBI ATTAAT 1 cut(s) 405
Psp1406I AACGTT 1 cut(s) 753
Psp6I CCWGG 4 cut(s) 47, 290, 589, 790
PspGI CCWGG 4 cut(s) 47, 290, 589, 790
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 2 cut(s) 276, 710
PstI CTGCAG 1 cut(s) 541
PstNI CAGNNNCTG 2 cut(s) 125, 773
PvuII CAGCTG 1 cut(s) 697
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 5 cut(s) 438, 480, 537, 698, 768
Sau3AI GATC 5 cut(s) 24, 35, 214, 268, 775
Sau96I GGNCC 2 cut(s) 276, 710
SchI GAGTC 3 cut(s) 74, 329, 601
ScrFI CCNGG 4 cut(s) 49, 292, 591, 792
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 537
Sse9I AATT 5 cut(s) 385, 429, 499, 560, 813
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 1 cut(s) 19
SspMI CTAG 1 cut(s) 84
StuI AGGCCT 1 cut(s) 589
StyD4I CCNGG 4 cut(s) 47, 290, 589, 790
StyI CCWWGG 1 cut(s) 272
TaaI ACNGT 2 cut(s) 10, 127
TaiI ACGT 2 cut(s) 353, 756
TaqI TCGA 3 cut(s) 339, 610, 836
TasI AATT 5 cut(s) 385, 429, 499, 560, 813
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 3 cut(s) 663, 735, 750
TseFI GTSAC 2 cut(s) 286, 346
TseI GCWGC 5 cut(s) 437, 479, 536, 697, 767
Tsp45I GTSAC 2 cut(s) 286, 346
TspDTI ATGAA 1 cut(s) 111
TspRI CASTG 3 cut(s) 663, 735, 750
Van91I CCANNNNNTGG 1 cut(s) 707
VspI ATTAAT 1 cut(s) 405
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 2 cut(s) 560, 813
XceI RCATGY 2 cut(s) 197, 397
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.