AT5G11730

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005609
Physical Location & Seq
Forward (+)
3780302 .. 3782793
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G11730.1

Sequence Viewer

Length: 1161 bp
ATGCAGAATAGGATTGTACAGTTGGAAGAAGGAAAAGAAAATGGAATCACAGTTCGATCAGGGCATTATAAAGGATTTCCATCGAAACTGCTACTTCTGCTAGGGTTGTTTCTTACATTTTCTGTTACTGTCTTCATCATTAGTGTATCTACTATCAAGTATACTGGAATACAAAGTGTAGTAACCACAGTCACTTCAAGCTTTGTGCCATGCCGTGAGGGGGAACCGAATAGTTTGAGTAAATGGATACAACCTCCTGCGGTTTTGATGCATAACATGAGCGATGAAGAGCTTCTTTGGCGTGCTTCTTTTTGGCCTCGTAGAAAAGAATATCCGTTTAAACGGGTTCCAAAGGTTGCGTTTATGTTCTTGACTAAAGGCCCCTTACCGCTTGCTTCGCTTTGGGAGAGGTTCTTGAAGGGTCACAAAGGGCTTTACTCGGTTTACCTTCATCCACACCCTTCTTTTACAGCCAAGTTTCCAGCTAGCTCTGTTTTCCACCGGAGGCAAATTCCAAGCCAGGTTGCAGAATGGGGGAGAATGTCCATGTGCGATGCAGAGAAGCGGCTTCTTGCCAACGCCCTGCTTGATGTGTCCAACGAATGGTTTGTTCTCGTCTCAGAGTCATGCATTCCACTCTACAACTTCACAACCATTTACAGCTACTTAAGCCGATCAAAACACAGCTTCATGGGTGCTTTTGATGACCCTGGACCGTTTGGGCGTGGCCGCTACAACGGGAACATGGAACCTGAAGTTCCCCTTACCAAATGGCGAAAAGGGTCTCAATGGTTTGAGGTTAACCGGGACCTGGCAGCTACCATTGTCAAAGACACATTGTATTACCCGAAATTCAAAGAGTTCTGCAGGCCTGCTTGCTATGTGGACGAGCATTACTTCCCAACAATGTTGACGATTGAGAAACCAACAGTCTTGGCTAACCGGAGCTTGACATGGGTGGACTGGTCGAGGGGCGGTCCCCACCCAGCTACGTTTGGGCGATCGGACATTACAGAAAACTTCTTTGGAAAAATCTTTGATGGAAGAAACTGCAGTTACAATGGTCGCAACACTTCCATGTGTTATCTCTTTGCGAGGAAGTTTGCTCCGAGTGCTTTGGAGCCTTTGCTTCACATTGCTCCCAAGATTCTTGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

386

Amino Acids

44.27

Weight (kDa)

9.47

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 119 - 345 1.9e-86 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 603
AccI GTMKAC 1 cut(s) 161
AciI CCGC 5 cut(s) 260, 389, 565, 730, 975
AcoI YGGCCR 1 cut(s) 727
AcsI RAATTY 2 cut(s) 510, 851
AcuI CTGAAG 1 cut(s) 774
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 3 cut(s) 220, 603, 811
AflII CTTAAG 1 cut(s) 667
AgsI TTSAA 3 cut(s) 198, 418, 856
AjnI CCWGG 3 cut(s) 519, 709, 810
AjuI GAANNNNNNNTTGG 2 cut(s) 1008, 1040
AloI GAACNNNNNNTCC 2 cut(s) 36, 68
AluBI AGCT 9 cut(s) 201, 292, 485, 489, 663, 687, 818, 948, 989
AluI AGCT 9 cut(s) 201, 292, 485, 489, 663, 687, 818, 948, 989
Alw26I GTCTC 2 cut(s) 622, 789
AoxI GGCC 4 cut(s) 314, 379, 727, 869
ApeKI GCWGC 1 cut(s) 815
ApoI RAATTY 2 cut(s) 510, 851
Asp700I GAANNNNTTC 1 cut(s) 291
AspS9I GGNCC 4 cut(s) 380, 713, 808, 977
AsuC2I CCSGG 1 cut(s) 806
AsuNHI GCTAGC 1 cut(s) 485
AvaII GGWCC 3 cut(s) 713, 808, 977
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 827
BccI CCATC 2 cut(s) 88, 1034
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 3 cut(s) 521, 711, 812
BciVI GTATCC 1 cut(s) 240
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 2 cut(s) 622, 789
BfaI CTAG 2 cut(s) 101, 486
BfmI CTRYAG 2 cut(s) 865, 1051
BfrI CTTAAG 1 cut(s) 667
BfuI GTATCC 1 cut(s) 240
BisI GCNGC 3 cut(s) 566, 730, 816
BlsI GCNGC 3 cut(s) 567, 731, 817
Bme1390I CCNGG 4 cut(s) 521, 711, 806, 812
Bme18I GGWCC 3 cut(s) 713, 808, 977
BmgT120I GGNCC 4 cut(s) 380, 713, 808, 977
BmiI GGNNCC 7 cut(s) 225, 348, 382, 750, 809, 979, 1122
BmrFI CCNGG 4 cut(s) 521, 711, 806, 812
BmsI GCATC 2 cut(s) 258, 544
BmtI GCTAGC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 806
BsaBI GATNNNNATC 1 cut(s) 79
BsaI GGTCTC 1 cut(s) 789
BsaJI CCNNGG 1 cut(s) 709
BsaWI WCCGGW 2 cut(s) 501, 942
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 603, 811
Bse1I ACTGG 2 cut(s) 169, 968
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 79
BseBI CCWGG 3 cut(s) 521, 711, 812
BseDI CCNNGG 1 cut(s) 709
BseGI GGATG 1 cut(s) 451
BseJI GATNNNNATC 1 cut(s) 79
BseLI CCNNNNNNNGG 3 cut(s) 220, 603, 811
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 2 cut(s) 169, 968
BseXI GCAGC 1 cut(s) 827
BseYI CCCAGC 1 cut(s) 985
Bsh1285I CGRYCG 1 cut(s) 1004
BshFI GGCC 4 cut(s) 316, 381, 729, 871
BsiEI CGRYCG 1 cut(s) 1004
BsiSI CCGG 3 cut(s) 502, 805, 943
BslFI GGGAC 2 cut(s) 821, 963
BslI CCNNNNNNNGG 3 cut(s) 220, 603, 811
BsmAI GTCTC 2 cut(s) 622, 789
BsmBI CGTCTC 1 cut(s) 622
BsmFI GGGAC 2 cut(s) 821, 963
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 4 cut(s) 316, 381, 729, 871
Bso31I GGTCTC 1 cut(s) 789
Bsp1407I TGTACA 1 cut(s) 16
Bsp143I GATC 3 cut(s) 56, 674, 1001
BspACI CCGC 5 cut(s) 260, 389, 565, 730, 975
BspANI GGCC 4 cut(s) 316, 381, 729, 871
BspCNI CTCAG 1 cut(s) 632
BspLI GGNNCC 7 cut(s) 225, 348, 382, 750, 809, 979, 1122
BspMAI CTGCAG 2 cut(s) 869, 1055
BspOI GCTAGC 1 cut(s) 489
BspQI GCTCTTC 1 cut(s) 282
BspTI CTTAAG 1 cut(s) 667
BspTNI GGTCTC 1 cut(s) 789
BsrDI GCAATG 1 cut(s) 1134
BsrGI TGTACA 1 cut(s) 16
BsrI ACTGG 2 cut(s) 169, 968
BssECI CCNNGG 1 cut(s) 709
BssMI GATC 3 cut(s) 56, 674, 1001
BssNAI GTATAC 1 cut(s) 162
Bst1107I GTATAC 1 cut(s) 162
Bst2UI CCWGG 3 cut(s) 521, 711, 812
Bst4CI ACNGT 6 cut(s) 21, 52, 130, 190, 717, 931
Bst6I CTCTTC 1 cut(s) 282
BstAFI CTTAAG 1 cut(s) 667
BstAUI TGTACA 1 cut(s) 16
BstC8I GCNNGC 6 cut(s) 303, 393, 487, 869, 873, 877
BstDEI CTNAG 1 cut(s) 619
BstF5I GGATG 1 cut(s) 451
BstKTI GATC 3 cut(s) 59, 677, 1004
BstMAI GTCTC 2 cut(s) 622, 789
BstMBI GATC 3 cut(s) 56, 674, 1001
BstMCI CGRYCG 1 cut(s) 1004
BstMWI GCNNNNNNNGC 5 cut(s) 97, 298, 397, 669, 1112
BstNI CCWGG 3 cut(s) 521, 711, 812
BstSCI CCNGG 4 cut(s) 519, 709, 804, 810
BstSFI CTRYAG 2 cut(s) 865, 1051
BstV1I GCAGC 1 cut(s) 827
BstV2I GAAGAC 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 162
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 4 cut(s) 316, 381, 729, 871
BtgZI GCGATG 2 cut(s) 297, 567
BtsCI GGATG 1 cut(s) 451
Cac8I GCNNGC 6 cut(s) 303, 393, 487, 869, 873, 877
Cfr13I GGNCC 4 cut(s) 380, 713, 808, 977
Csp6I GTAC 1 cut(s) 17
CviAII CATG 8 cut(s) 210, 277, 547, 627, 691, 745, 954, 1078
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 1 cut(s) 619
DpnI GATC 3 cut(s) 58, 676, 1003
DpnII GATC 3 cut(s) 56, 674, 1001
DraI TTTAAA 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 727
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 871
Eco31I GGTCTC 1 cut(s) 789
Eco47I GGWCC 3 cut(s) 713, 808, 977
Eco57I CTGAAG 1 cut(s) 774
EcoO109I RGGNCCY 2 cut(s) 380, 808
EcoRII CCWGG 3 cut(s) 519, 709, 810
EcoT22I ATGCAT 2 cut(s) 273, 632
Esp3I CGTCTC 1 cut(s) 622
FaeI CATG 8 cut(s) 213, 280, 550, 630, 694, 748, 957, 1081
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 747, 779
FaqI GGGAC 2 cut(s) 821, 963
FatI CATG 8 cut(s) 209, 276, 546, 626, 690, 744, 953, 1077
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 566, 730, 816
FokI GGATG 1 cut(s) 438
Fsp4HI GCNGC 3 cut(s) 566, 730, 816
FspBI CTAG 2 cut(s) 101, 486
GluI GCNGC 3 cut(s) 566, 730, 816
GsaI CCCAGC 1 cut(s) 989
HaeIII GGCC 4 cut(s) 316, 381, 729, 871
HapII CCGG 3 cut(s) 502, 805, 943
Hin1II CATG 8 cut(s) 213, 280, 550, 630, 694, 748, 957, 1081
HincII GTYRAC 2 cut(s) 802, 912
HindII GTYRAC 2 cut(s) 802, 912
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 3 cut(s) 45, 623, 1147
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 3 cut(s) 502, 805, 943
Hpy166II GTNNAC 6 cut(s) 162, 445, 802, 886, 912, 961
Hpy188I TCNGA 3 cut(s) 622, 1006, 1110
Hpy188III TCNNGA 2 cut(s) 370, 415
Hpy8I GTNNAC 6 cut(s) 162, 445, 802, 886, 912, 961
HpyAV CCTTC 4 cut(s) 23, 412, 458, 471
HpyCH4III ACNGT 6 cut(s) 21, 52, 130, 190, 717, 931
HpyCH4IV ACGT 1 cut(s) 992
HpyCH4V TGCA 7 cut(s) 4, 271, 527, 557, 630, 867, 1053
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 298, 397, 669, 1112
HpyF3I CTNAG 1 cut(s) 619
HpySE526I ACGT 1 cut(s) 992
Hsp92II CATG 8 cut(s) 213, 280, 550, 630, 694, 748, 957, 1081
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 3 cut(s) 56, 674, 1001
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 4 cut(s) 945, 1111, 1120, 1144
Lsp1109I GCAGC 1 cut(s) 827
LweI GCATC 2 cut(s) 258, 544
MaeI CTAG 2 cut(s) 101, 486
MaeII ACGT 1 cut(s) 992
MaeIII GTNAC 5 cut(s) 124, 181, 190, 422, 1055
MalI GATC 3 cut(s) 58, 676, 1003
MboI GATC 3 cut(s) 56, 674, 1001
MboII GAAGA 4 cut(s) 38, 124, 299, 1056
MluCI AATT 2 cut(s) 510, 851
MlyI GAGTC 1 cut(s) 632
MmeI TCCRAC 1 cut(s) 621
MnlI CCTC 8 cut(s) 211, 264, 327, 402, 498, 790, 963, 1089
Mph1103I ATGCAT 2 cut(s) 273, 632
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 3 cut(s) 339, 668, 801
MslI CAYNNNNRTG 1 cut(s) 1076
MspCI CTTAAG 1 cut(s) 667
MspI CCGG 3 cut(s) 502, 805, 943
MspR9I CCNGG 4 cut(s) 521, 711, 806, 812
MssI GTTTAAAC 1 cut(s) 340
Mva1269I GAATGC 1 cut(s) 630
MvaI CCWGG 3 cut(s) 521, 711, 812
MwoI GCNNNNNNNGC 5 cut(s) 97, 298, 397, 669, 1112
NciI CCSGG 1 cut(s) 806
NdeII GATC 3 cut(s) 56, 674, 1001
NheI GCTAGC 1 cut(s) 485
NlaIII CATG 8 cut(s) 213, 280, 550, 630, 694, 748, 957, 1081
NlaIV GGNNCC 7 cut(s) 225, 348, 382, 750, 809, 979, 1122
NmuCI GTSAC 2 cut(s) 190, 422
NsiI ATGCAT 2 cut(s) 273, 632
PceI AGGCCT 1 cut(s) 871
PciSI GCTCTTC 1 cut(s) 282
PctI GAATGC 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 2 cut(s) 45, 1147
PflMI CCANNNNNTGG 1 cut(s) 603
PkrI GCNGC 3 cut(s) 567, 731, 817
Ple19I CGATCG 1 cut(s) 1004
PleI GAGTC 1 cut(s) 631
PmeI GTTTAAAC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 631
PpuMI RGGWCCY 1 cut(s) 808
PsiI TTATAA 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 3 cut(s) 519, 709, 810
PspFI CCCAGC 1 cut(s) 985
PspGI CCWGG 3 cut(s) 519, 709, 810
PspN4I GGNNCC 7 cut(s) 225, 348, 382, 750, 809, 979, 1122
PspPI GGNCC 4 cut(s) 380, 713, 808, 977
PspPPI RGGWCCY 1 cut(s) 808
PstI CTGCAG 2 cut(s) 869, 1055
PvuI CGATCG 1 cut(s) 1004
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 1076
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 3 cut(s) 339, 668, 801
SatI GCNGC 3 cut(s) 566, 730, 816
Sau3AI GATC 3 cut(s) 56, 674, 1001
Sau96I GGNCC 4 cut(s) 380, 713, 808, 977
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 4 cut(s) 521, 711, 806, 812
SfaNI GCATC 2 cut(s) 258, 544
SfcI CTRYAG 2 cut(s) 865, 1051
SinI GGWCC 3 cut(s) 713, 808, 977
SmiMI CAYNNNNRTG 1 cut(s) 1076
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 2 cut(s) 510, 851
SseBI AGGCCT 1 cut(s) 871
SsiI CCGC 5 cut(s) 260, 389, 565, 730, 975
SspMI CTAG 2 cut(s) 101, 486
StuI AGGCCT 1 cut(s) 871
StyD4I CCNGG 4 cut(s) 519, 709, 804, 810
TaaI ACNGT 6 cut(s) 21, 52, 130, 190, 717, 931
TaiI ACGT 1 cut(s) 995
TaqI TCGA 3 cut(s) 55, 83, 968
TasI AATT 2 cut(s) 510, 851
TatI WGTACW 1 cut(s) 16
TauI GCSGC 2 cut(s) 568, 732
TfiI GAWTC 2 cut(s) 45, 1147
Tru1I TTAA 3 cut(s) 339, 668, 801
Tru9I TTAA 3 cut(s) 339, 668, 801
TseFI GTSAC 2 cut(s) 190, 422
TseI GCWGC 1 cut(s) 815
Tsp45I GTSAC 2 cut(s) 190, 422
TspDTI ATGAA 4 cut(s) 124, 300, 440, 679
TspGWI ACGGA 1 cut(s) 324
Van91I CCANNNNNTGG 1 cut(s) 603
Vha464I CTTAAG 1 cut(s) 667
VpaK11BI GGWCC 3 cut(s) 713, 808, 977
XapI RAATTY 2 cut(s) 510, 851
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 2 cut(s) 101, 486
Zsp2I ATGCAT 2 cut(s) 273, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.