AT5G25970

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005609
Physical Location & Seq
Forward (+)
9066278 .. 9068511
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25970.1

Sequence Viewer

Length: 1164 bp
ATGCAGTGTAGTAGGGTTCTTCAGTTGGAAGAAGGTAAAGAGATTGTAGTTACAAGCAGCCGGACCGGGCTTACTAAGGCGTTTCCTTACAAGTTGCTTCTCTTGCTAGGTTTTTTTCTAGCTTTTACTGTTGTTTTATTCTTCATTAGTGTATCCACCATCAAGTATTATGGAATCAATAGTGTAGTGACTTCAGTCACTTCTAGTTTTGTGCCTTGCCACGAGAAAATAAATGATCTTGATAAATGGATCAAACCTCTTGTGGTTCTCATGCATAATATGAGCGATGAAGAGCTTCTTTGGGGTGCATCTTTCATGCCTCGGAGGAAAGAGTACCCGTTTAATCGGGTCCCTAAAATCGCGTTTATGTTCTTGACAATGGGTCCTTTGCCTCTTGCACCGCTTTGGGAGAGGTTATTAAAGGGTCATGAGAAGCTTTACTCAGTTTATATCCATTCACCTGTATCTTCTTCAGCTAAGTTTCCAGCTTCCTCTGTTTTCTACCGTAGGCATATACCAAGTCAGGTTGCAGAATGGGGAAGAATGACAATGTGCGATGCAGAGAGGCGGCTTCTTGCAAATGCGCTACTCGATATCTCAAACGAATGGTTTGTTCTCCTCTCCGAGTCATGCATTCCCCTATTCAACTTCACAACTATCTACACGTACATGACCAAATCAGAGCATAGCTTTATGGGTTCATTTGATGATCCTGGTGCATATGGCAGAGGCCGCTACCACGGCAACATGGCCCCTGAAGTTTTCATAGACCAATGGAGAAAAGGGTCTCAATGGTTTGAGATTAACCGGGAGCTTGCGGTTTCCATTGTCAAAGATACATTATATTACCCTAAATTCAAAGAGTTTTGTCAACCTGCTTGTTATGTGGACGAGCATTATTTCCCAACAATGTTGACAATTGAGAAACCGGCCGCCTTGGCTAACCGAAGTGTGACTTGGGTCGACTGGTCGAGAGGTGGTGCCCACCCAGCTACATTTGGAGCACAAGACATCAATGAGGAGTTCTTTGCCAGAATTCTCAAAGGTGACAACTGCACTTACAATGGTGGCTACACATCCATGTGTTATCTCTTTGCTAGAAAGTTTTCTCCTAGTGCTTTGGAGCCTTTGGTTCAAATTGCACCCAAACTTTTGAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

387

Amino Acids

44.33

Weight (kDa)

8.49

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 120 - 346 2e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 883
AccB1I GGYRCC 1 cut(s) 980
AccI GTMKAC 1 cut(s) 963
AccII CGCG 1 cut(s) 362
AciI CCGC 5 cut(s) 401, 568, 733, 818, 933
AclWI GGATC 2 cut(s) 257, 704
AcoI YGGCCR 1 cut(s) 930
AcsI RAATTY 2 cut(s) 854, 1035
AcuI CTGAAG 4 cut(s) 5, 177, 456, 777
AfaI GTAC 2 cut(s) 335, 668
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 3 cut(s) 646, 859, 1136
AhdI GACNNNNNGTC 1 cut(s) 381
AjnI CCWGG 1 cut(s) 712
AjuI GAANNNNNNNTTGG 2 cut(s) 940, 972
AluBI AGCT 8 cut(s) 122, 295, 436, 476, 488, 690, 814, 992
AluI AGCT 8 cut(s) 122, 295, 436, 476, 488, 690, 814, 992
Alw21I GWGCWC 1 cut(s) 1006
Alw26I GTCTC 1 cut(s) 792
AlwI GGATC 2 cut(s) 257, 704
AoxI GGCC 3 cut(s) 730, 750, 930
ApeKI GCWGC 1 cut(s) 57
ApoI RAATTY 2 cut(s) 854, 1035
Asp700I GAANNNNTTC 2 cut(s) 294, 1105
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 4 cut(s) 63, 349, 383, 751
AsuC2I CCSGG 2 cut(s) 67, 809
AsuHPI GGTGA 2 cut(s) 450, 1058
AvaII GGWCC 3 cut(s) 63, 349, 383
BaeGI GKGCMC 1 cut(s) 985
BanI GGYRCC 1 cut(s) 980
BauI CACGAG 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 1006
BbvI GCAGC 1 cut(s) 69
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 757
BciT130I CCWGG 1 cut(s) 714
BciVI GTATCC 1 cut(s) 163
BcnI CCSGG 2 cut(s) 67, 809
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 5 cut(s) 107, 119, 204, 1098, 1113
BfuAI ACCTGC 1 cut(s) 883
BfuI GTATCC 1 cut(s) 163
BglI GCCNNNNNGGC 1 cut(s) 938
BisI GCNGC 4 cut(s) 58, 569, 733, 933
BlsI GCNGC 4 cut(s) 59, 570, 734, 934
Bme1390I CCNGG 3 cut(s) 67, 714, 809
Bme18I GGWCC 3 cut(s) 63, 349, 383
BmeRI GACNNNNNGTC 1 cut(s) 381
BmgT120I GGNCC 4 cut(s) 63, 349, 383, 751
BmiI GGNNCC 6 cut(s) 350, 351, 384, 753, 982, 1125
BmrFI CCNGG 3 cut(s) 67, 714, 809
BmsI GCATC 2 cut(s) 317, 547
BoxI GACNNNNGTC 2 cut(s) 194, 959
BpuMI CCSGG 2 cut(s) 67, 809
BsaAI YACGTR 1 cut(s) 666
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 3 cut(s) 320, 739, 936
Bse118I RCCGGY 1 cut(s) 928
Bse1I ACTGG 1 cut(s) 971
BseBI CCWGG 1 cut(s) 714
BseDI CCNNGG 3 cut(s) 320, 739, 936
BseGI GGATG 1 cut(s) 1076
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 971
BseRI GAGGAG 2 cut(s) 608, 1034
BseSI GKGCMC 1 cut(s) 985
BseX3I CGGCCG 1 cut(s) 930
BseXI GCAGC 1 cut(s) 69
BseYI CCCAGC 1 cut(s) 988
BsgI GTGCAG 1 cut(s) 1039
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 933
BshFI GGCC 3 cut(s) 732, 752, 932
BshNI GGYRCC 1 cut(s) 980
BsiEI CGRYCG 1 cut(s) 933
BsiHKAI GWGCWC 1 cut(s) 1006
BsiSI CCGG 4 cut(s) 61, 66, 808, 929
BslFI GGGAC 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 792
BsmFI GGGAC 1 cut(s) 335
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 3 cut(s) 732, 752, 932
Bso31I GGTCTC 1 cut(s) 792
Bsp1286I GDGCHC 2 cut(s) 985, 1006
Bsp143I GATC 3 cut(s) 235, 249, 709
BspACI CCGC 5 cut(s) 401, 568, 733, 818, 933
BspANI GGCC 3 cut(s) 732, 752, 932
BspCNI CTCAG 1 cut(s) 455
BspFNI CGCG 1 cut(s) 362
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 6 cut(s) 350, 351, 384, 753, 982, 1125
BspMI ACCTGC 1 cut(s) 883
BspPI GGATC 2 cut(s) 257, 704
BspQI GCTCTTC 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 980
BspTNI GGTCTC 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 928
BsrI ACTGG 1 cut(s) 971
BssAI RCCGGY 1 cut(s) 928
BssECI CCNNGG 3 cut(s) 320, 739, 936
BssMI GATC 3 cut(s) 235, 249, 709
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 936
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 2 cut(s) 130, 506
Bst6I CTCTTC 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 666
BstC8I GCNNGC 1 cut(s) 816
BstDEI CTNAG 3 cut(s) 75, 442, 477
BstDSI CCRYGG 1 cut(s) 739
BstF5I GGATG 1 cut(s) 1076
BstFNI CGCG 1 cut(s) 362
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 3 cut(s) 238, 252, 712
BstMAI GTCTC 1 cut(s) 792
BstMBI GATC 3 cut(s) 235, 249, 709
BstMCI CGRYCG 1 cut(s) 933
BstMWI GCNNNNNNNGC 5 cut(s) 103, 732, 741, 938, 989
BstNI CCWGG 1 cut(s) 714
BstPAI GACNNNNGTC 2 cut(s) 194, 959
BstSCI CCNGG 3 cut(s) 65, 712, 807
BstSLI GKGCMC 1 cut(s) 985
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 1 cut(s) 69
BstZI CGGCCG 1 cut(s) 930
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 3 cut(s) 732, 752, 932
BtgI CCRYGG 1 cut(s) 739
BtgZI GCGATG 2 cut(s) 300, 570
BtsCI GGATG 1 cut(s) 1076
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 1 cut(s) 11
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 1 cut(s) 816
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 928
Cfr13I GGNCC 4 cut(s) 63, 349, 383, 751
CpoI CGGWCCG 1 cut(s) 63
Csp6I GTAC 2 cut(s) 334, 667
CspI CGGWCCG 1 cut(s) 63
CviAII CATG 7 cut(s) 271, 316, 428, 630, 670, 748, 1081
CviQI GTAC 2 cut(s) 334, 667
DdeI CTNAG 3 cut(s) 75, 442, 477
DpnI GATC 3 cut(s) 237, 251, 711
DpnII GATC 3 cut(s) 235, 249, 709
DriI GACNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 930
EagI CGGCCG 1 cut(s) 930
Eam1104I CTCTTC 1 cut(s) 285
Eam1105I GACNNNNNGTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 285
EclXI CGGCCG 1 cut(s) 930
Eco130I CCWWGG 1 cut(s) 936
Eco31I GGTCTC 1 cut(s) 792
Eco32I GATATC 1 cut(s) 595
Eco47I GGWCC 3 cut(s) 63, 349, 383
Eco52I CGGCCG 1 cut(s) 930
Eco57I CTGAAG 4 cut(s) 5, 177, 456, 777
EcoO109I RGGNCCY 2 cut(s) 349, 383
EcoRI GAATTC 1 cut(s) 1035
EcoRII CCWGG 1 cut(s) 712
EcoRV GATATC 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 2 cut(s) 276, 635
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 7 cut(s) 274, 319, 431, 633, 673, 751, 1084
FalI AAGNNNNNCTT 4 cut(s) 282, 314, 940, 972
FaqI GGGAC 1 cut(s) 335
FatI CATG 7 cut(s) 270, 315, 427, 629, 669, 747, 1080
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 1 cut(s) 963
Fnu4HI GCNGC 4 cut(s) 58, 569, 733, 933
FokI GGATG 1 cut(s) 1063
Fsp4HI GCNGC 4 cut(s) 58, 569, 733, 933
FspBI CTAG 5 cut(s) 107, 119, 204, 1098, 1113
GlaI GCGC 1 cut(s) 585
GluI GCNGC 4 cut(s) 58, 569, 733, 933
GsaI CCCAGC 1 cut(s) 992
HaeIII GGCC 3 cut(s) 732, 752, 932
HapII CCGG 4 cut(s) 61, 66, 808, 929
HhaI GCGC 1 cut(s) 586
Hin1II CATG 7 cut(s) 274, 319, 431, 633, 673, 751, 1084
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HincII GTYRAC 3 cut(s) 872, 915, 964
HindII GTYRAC 3 cut(s) 872, 915, 964
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 2 cut(s) 174, 626
HpaII CCGG 4 cut(s) 61, 66, 808, 929
HphI GGTGA 2 cut(s) 450, 1058
Hpy166II GTNNAC 4 cut(s) 872, 889, 915, 964
Hpy188I TCNGA 3 cut(s) 324, 625, 682
Hpy188III TCNNGA 5 cut(s) 239, 373, 428, 972, 1161
Hpy8I GTNNAC 4 cut(s) 872, 889, 915, 964
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 2 cut(s) 130, 506
HpyCH4IV ACGT 1 cut(s) 665
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 732, 741, 938, 989
HpyF3I CTNAG 3 cut(s) 75, 442, 477
HpySE526I ACGT 1 cut(s) 665
Hsp92II CATG 7 cut(s) 274, 319, 431, 633, 673, 751, 1084
HspAI GCGC 1 cut(s) 584
KflI GGGWCCC 1 cut(s) 349
Kzo9I GATC 3 cut(s) 235, 249, 709
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 3 cut(s) 811, 1001, 1123
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 2 cut(s) 317, 547
MaeI CTAG 5 cut(s) 107, 119, 204, 1098, 1113
MaeII ACGT 1 cut(s) 665
MaeIII GTNAC 5 cut(s) 49, 187, 196, 952, 1046
MalI GATC 3 cut(s) 237, 251, 711
MboI GATC 3 cut(s) 235, 249, 709
MboII GAAGA 7 cut(s) 11, 41, 133, 302, 459, 462, 552
MfeI CAATTG 1 cut(s) 918
MhlI GDGCHC 2 cut(s) 985, 1006
MluCI AATT 4 cut(s) 854, 918, 1035, 1137
MlyI GAGTC 1 cut(s) 635
MmeI TCCRAC 1 cut(s) 6
Mph1103I ATGCAT 2 cut(s) 276, 635
MroXI GAANNNNTTC 2 cut(s) 294, 1105
MseI TTAA 3 cut(s) 342, 419, 804
MslI CAYNNNNRTG 3 cut(s) 668, 1079, 1081
MspI CCGG 4 cut(s) 61, 66, 808, 929
MspR9I CCNGG 3 cut(s) 67, 714, 809
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 5 cut(s) 103, 732, 741, 938, 989
NciI CCSGG 2 cut(s) 67, 809
NdeI CATATG 1 cut(s) 721
NdeII GATC 3 cut(s) 235, 249, 709
NlaIII CATG 7 cut(s) 274, 319, 431, 633, 673, 751, 1084
NlaIV GGNNCC 6 cut(s) 350, 351, 384, 753, 982, 1125
NmuCI GTSAC 4 cut(s) 187, 196, 952, 1046
NsiI ATGCAT 2 cut(s) 276, 635
PagI TCATGA 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 285
PctI GAATGC 1 cut(s) 633
PdmI GAANNNNTTC 2 cut(s) 294, 1105
PfeI GAWTC 1 cut(s) 174
PkrI GCNGC 4 cut(s) 59, 570, 734, 934
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
Ppu21I YACGTR 1 cut(s) 666
PpuMI RGGWCCY 2 cut(s) 349, 383
PshAI GACNNNNGTC 2 cut(s) 194, 959
Psp5II RGGWCCY 2 cut(s) 349, 383
Psp6I CCWGG 1 cut(s) 712
PspFI CCCAGC 1 cut(s) 988
PspGI CCWGG 1 cut(s) 712
PspN4I GGNNCC 6 cut(s) 350, 351, 384, 753, 982, 1125
PspPI GGNCC 4 cut(s) 63, 349, 383, 751
PspPPI RGGWCCY 2 cut(s) 349, 383
RsaI GTAC 2 cut(s) 335, 668
RsaNI GTAC 2 cut(s) 334, 667
RseI CAYNNNNRTG 3 cut(s) 668, 1079, 1081
Rsr2I CGGWCCG 1 cut(s) 63
RsrII CGGWCCG 1 cut(s) 63
SalI GTCGAC 1 cut(s) 962
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 3 cut(s) 342, 419, 804
SatI GCNGC 4 cut(s) 58, 569, 733, 933
Sau3AI GATC 3 cut(s) 235, 249, 709
Sau96I GGNCC 4 cut(s) 63, 349, 383, 751
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 3 cut(s) 67, 714, 809
SduI GDGCHC 2 cut(s) 985, 1006
SfaNI GCATC 2 cut(s) 317, 547
SinI GGWCC 3 cut(s) 63, 349, 383
SmiMI CAYNNNNRTG 3 cut(s) 668, 1079, 1081
Sse9I AATT 4 cut(s) 854, 918, 1035, 1137
SsiI CCGC 5 cut(s) 401, 568, 733, 818, 933
SspMI CTAG 5 cut(s) 107, 119, 204, 1098, 1113
StyD4I CCNGG 3 cut(s) 65, 712, 807
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 2 cut(s) 130, 506
TaiI ACGT 1 cut(s) 668
TaqI TCGA 3 cut(s) 591, 963, 971
TasI AATT 4 cut(s) 854, 918, 1035, 1137
TauI GCSGC 3 cut(s) 571, 735, 935
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 3 cut(s) 342, 419, 804
Tru9I TTAA 3 cut(s) 342, 419, 804
TseFI GTSAC 4 cut(s) 187, 196, 952, 1046
TseI GCWGC 1 cut(s) 57
Tsp45I GTSAC 4 cut(s) 187, 196, 952, 1046
TspDTI ATGAA 5 cut(s) 133, 303, 304, 690, 754
VpaK11BI GGWCC 3 cut(s) 63, 349, 383
XapI RAATTY 2 cut(s) 854, 1035
XmiI GTMKAC 1 cut(s) 963
XmnI GAANNNNTTC 2 cut(s) 294, 1105
XspI CTAG 5 cut(s) 107, 119, 204, 1098, 1113
Zsp2I ATGCAT 2 cut(s) 276, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.