FvH4_2g32250

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005609
Physical Location & Seq
Reverse (-)
24504912 .. 24508408
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32250.t2

Sequence Viewer

Length: 1170 bp
ATGCAAGTGATGCAATCAAGGGTTGTGCCATTGGAGGAAGGCAAGGACCCGGGAGTTACTACTAGCAGGACAAGCCCAACCAAGGCATTGCCGATTCGGCTGCTTCAGTTATTTGTTCTGTTTCTGGCTTTCTGTATTACTTTCTCAGTGATTAGTATATACACCATTCGGCATTTTGGGATTAGTAATGTGATGACTTCAGTCACGTCCTCCATCCAGCCATGTTTTGAGGAACCAATTGGCTTGGATAGATGGATTAAGCCGCCGTCGAATTTGATGCACACGATGAATGACAAAGAACTGTTGTGGAGGGCTTCTTTGACGCCTAGGAGAAAGGACTATCCTTTTGAGAGAGTTCCGAAGATTGCGCTTATGTTCTTGACTAAGGGACCTTTGCCTCTGGCACCAATTTGGGAGAGGTTTCTGAGGGGGCATCAAGGACTTTATTCAATCTATGTTCATTCATTGCCATCATTTCAACCCCATTTCCCAGCTTCGTCTGTTTTTCATAAGAGACATATTCCTAGCCAGGTGGCTGAATGGGGAAGGATGAGTATGTGTGATGCTGAGAGGAGACTTCTTGCTAATGCATTGCTTGATATATCCAATGAATGGTTCATTCTTCTTTCTGAATCATGCATTCCCCTCTATAACTTCACTGTGGTTTATGAGTACATGAGGAAGTCCAAGTACAGCTACATGGGTGCTTTTGATGACCATGGCCCGTTTGGCAGAGGACGCTACAATGATAACATGGCTCCTGAAGTGAACATTACCCAGTGGCGTAAGGGATCCCAGTGGTTTGAAGTTAACCGGAAGCTTGCTATCACCATTGTCGAAGACACAAAATTTTACCCAAAATTTAAAGAGTTCTGCAGACCAGCGTGTTATGTGGACGAGCACTACTTCCCCACCATGCTAACCATTCAAGCAGGAAGTATGATAGCAAACAGAAGCATCACTTGGGTGGATTGGTCAAGGGGCGGACCTCACCCTGCTACCTTCGGCAGAGGAGATATCACTGAGGATTTCATGAAGCGGATGCTCGGGAGTCATCGCTGCACTTACAACAACCAAAACTCTACAATGTGCTTTCTCTTTGCAAGAAAATTTGCTCCCAGTACCTTGGAACCTCTCTTACGTTTAGCACCAAAGTTTTTGGGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

390

Amino Acids

45.05

Weight (kDa)

9.39

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 122 - 348 5.8e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 403
AccB7I CCANNNNNTGG 1 cut(s) 612
AciI CCGC 3 cut(s) 263, 984, 1039
AclWI GGATC 2 cut(s) 786, 799
AcsI RAATTY 4 cut(s) 271, 848, 860, 1109
AcuI CTGAAG 3 cut(s) 89, 183, 783
AcyI GRCGYC 1 cut(s) 323
AfaI GTAC 3 cut(s) 674, 692, 1123
AfiI CCNNNNNNNGG 2 cut(s) 82, 612
AgsI TTSAA 4 cut(s) 450, 479, 806, 929
AjiI CACGTC 1 cut(s) 207
AjnI CCWGG 1 cut(s) 528
AjuI GAANNNNNNNTTGG 4 cut(s) 599, 631, 946, 978
AleI CACNNNNGTG 1 cut(s) 965
AluBI AGCT 3 cut(s) 494, 696, 820
AluI AGCT 3 cut(s) 494, 696, 820
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 2 cut(s) 508, 568
AlwI GGATC 2 cut(s) 786, 799
Ama87I CYCGRG 2 cut(s) 49, 1046
AoxI GGCC 1 cut(s) 721
ApeKI GCWGC 2 cut(s) 100, 1059
ApoI RAATTY 4 cut(s) 271, 848, 860, 1109
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 1 cut(s) 370
AspS9I GGNCC 4 cut(s) 46, 389, 722, 986
AsuC2I CCSGG 2 cut(s) 50, 51
AsuHPI GGTGA 2 cut(s) 820, 983
AvaI CYCGRG 2 cut(s) 49, 1046
AvaII GGWCC 3 cut(s) 46, 389, 986
AvrII CCTAGG 1 cut(s) 326
BamHI GGATCC 1 cut(s) 791
BanI GGYRCC 1 cut(s) 403
BarI GAAGNNNNNNTAC 2 cut(s) 674, 706
BbsI GAAGAC 1 cut(s) 846
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 2 cut(s) 87, 1046
BccI CCATC 3 cut(s) 221, 246, 478
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 4 cut(s) 82, 116, 259, 293
BciT130I CCWGG 1 cut(s) 530
BcnI CCSGG 2 cut(s) 50, 51
BcoDI GTCTC 2 cut(s) 508, 568
BfaI CTAG 3 cut(s) 63, 327, 525
BfmI CTRYAG 1 cut(s) 874
BglI GCCNNNNNGGC 2 cut(s) 97, 729
BisI GCNGC 3 cut(s) 101, 263, 1060
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 3 cut(s) 102, 264, 1061
Bme1390I CCNGG 3 cut(s) 50, 51, 530
Bme18I GGWCC 3 cut(s) 46, 389, 986
BmeT110I CYCGRG 2 cut(s) 49, 1046
BmgBI CACGTC 1 cut(s) 207
BmgT120I GGNCC 4 cut(s) 46, 389, 722, 986
BmiI GGNNCC 7 cut(s) 48, 234, 390, 405, 759, 793, 1131
BmrFI CCNGG 3 cut(s) 50, 51, 530
BmrI ACTGGG 3 cut(s) 772, 790, 1113
BmsI GCATC 5 cut(s) 267, 442, 553, 966, 1032
BmuI ACTGGG 3 cut(s) 772, 790, 1113
BoxI GACNNNNGTC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 846
BpuMI CCSGG 2 cut(s) 50, 51
BsaHI GRCGYC 1 cut(s) 323
BsaJI CCNNGG 5 cut(s) 49, 81, 326, 718, 1125
BsaWI WCCGGW 1 cut(s) 813
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 612
Bse1I ACTGG 3 cut(s) 778, 796, 1119
Bse3DI GCAATG 3 cut(s) 86, 464, 590
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 5 cut(s) 49, 81, 326, 718, 1125
BseGI GGATG 3 cut(s) 213, 555, 1047
BseLI CCNNNNNNNGG 2 cut(s) 82, 612
BseMI GCAATG 3 cut(s) 86, 464, 590
BseMII CTCAG 4 cut(s) 159, 416, 558, 1014
BseNI ACTGG 3 cut(s) 778, 796, 1119
BseRI GAGGAG 2 cut(s) 586, 1026
BseXI GCAGC 2 cut(s) 87, 1046
BseYI CCCAGC 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 1045
BshFI GGCC 1 cut(s) 723
BshNI GGYRCC 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 2 cut(s) 49, 1046
BsiSI CCGG 2 cut(s) 50, 814
BslFI GGGAC 1 cut(s) 402
BslI CCNNNNNNNGG 2 cut(s) 82, 612
BsmAI GTCTC 2 cut(s) 508, 568
BsmFI GGGAC 1 cut(s) 402
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 1 cut(s) 723
BsoBI CYCGRG 2 cut(s) 49, 1046
Bsp1286I GDGCHC 1 cut(s) 903
Bsp143I GATC 1 cut(s) 791
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 3 cut(s) 263, 984, 1039
BspANI GGCC 1 cut(s) 723
BspCNI CTCAG 4 cut(s) 158, 417, 559, 1015
BspHI TCATGA 1 cut(s) 1032
BspLI GGNNCC 7 cut(s) 48, 234, 390, 405, 759, 793, 1131
BspMAI CTGCAG 1 cut(s) 878
BspPI GGATC 2 cut(s) 786, 799
BspT107I GGYRCC 1 cut(s) 403
BsrDI GCAATG 3 cut(s) 86, 464, 590
BsrI ACTGG 3 cut(s) 778, 796, 1119
BssECI CCNNGG 5 cut(s) 49, 81, 326, 718, 1125
BssMI GATC 1 cut(s) 791
BssNI GRCGYC 1 cut(s) 323
BssT1I CCWWGG 4 cut(s) 81, 326, 718, 1125
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 2 cut(s) 303, 661
BstACI GRCGYC 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 5 cut(s) 145, 384, 425, 567, 1023
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 3 cut(s) 213, 555, 1047
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 1 cut(s) 794
BstMAI GTCTC 2 cut(s) 508, 568
BstMBI GATC 1 cut(s) 791
BstMWI GCNNNNNNNGC 5 cut(s) 10, 72, 97, 729, 738
BstNI CCWGG 1 cut(s) 530
BstPAI GACNNNNGTC 1 cut(s) 200
BstSCI CCNGG 3 cut(s) 48, 49, 528
BstSFI CTRYAG 1 cut(s) 874
BstV1I GCAGC 2 cut(s) 87, 1046
BstV2I GAAGAC 1 cut(s) 846
BstX2I RGATCY 1 cut(s) 791
BstXI CCANNNNNNTGG 1 cut(s) 1126
BstYI RGATCY 1 cut(s) 791
BsuRI GGCC 1 cut(s) 723
BtgI CCRYGG 1 cut(s) 718
BtgZI GCGATG 1 cut(s) 1040
BtrI CACGTC 1 cut(s) 207
BtsCI GGATG 3 cut(s) 213, 555, 1047
BtsIMutI CAGTG 5 cut(s) 153, 657, 785, 803, 1020
Cac8I GCNNGC 1 cut(s) 822
CciI TCATGA 1 cut(s) 1032
CfoI GCGC 1 cut(s) 370
Cfr13I GGNCC 4 cut(s) 46, 389, 722, 986
Cfr9I CCCGGG 1 cut(s) 49
CseI GACGC 2 cut(s) 331, 747
Csp6I GTAC 3 cut(s) 673, 691, 1122
CviAII CATG 8 cut(s) 222, 636, 676, 700, 719, 754, 916, 1033
CviQI GTAC 3 cut(s) 673, 691, 1122
DdeI CTNAG 5 cut(s) 145, 384, 425, 567, 1023
DpnI GATC 1 cut(s) 793
DpnII GATC 1 cut(s) 791
DraI TTTAAA 1 cut(s) 865
EciI GGCGGA 1 cut(s) 999
Eco130I CCWWGG 4 cut(s) 81, 326, 718, 1125
Eco32I GATATC 1 cut(s) 1018
Eco47I GGWCC 3 cut(s) 46, 389, 986
Eco57I CTGAAG 3 cut(s) 89, 183, 783
Eco88I CYCGRG 2 cut(s) 49, 1046
EcoO109I RGGNCCY 2 cut(s) 46, 389
EcoRII CCWGG 1 cut(s) 528
EcoRV GATATC 1 cut(s) 1018
EcoT14I CCWWGG 4 cut(s) 81, 326, 718, 1125
EcoT22I ATGCAT 2 cut(s) 592, 641
ErhI CCWWGG 4 cut(s) 81, 326, 718, 1125
FaeI CATG 8 cut(s) 225, 639, 679, 703, 722, 757, 919, 1036
FalI AAGNNNNNCTT 2 cut(s) 946, 978
FaqI GGGAC 1 cut(s) 402
FatI CATG 8 cut(s) 221, 635, 675, 699, 718, 753, 915, 1032
Fnu4HI GCNGC 3 cut(s) 101, 263, 1060
FokI GGATG 3 cut(s) 200, 562, 1054
Fsp4HI GCNGC 3 cut(s) 101, 263, 1060
FspBI CTAG 3 cut(s) 63, 327, 525
GlaI GCGC 1 cut(s) 369
GluI GCNGC 3 cut(s) 101, 263, 1060
GsaI CCCAGC 1 cut(s) 494
HaeIII GGCC 1 cut(s) 723
HapII CCGG 2 cut(s) 50, 814
HgaI GACGC 2 cut(s) 331, 747
HhaI GCGC 1 cut(s) 370
Hin1I GRCGYC 1 cut(s) 323
Hin1II CATG 8 cut(s) 225, 639, 679, 703, 722, 757, 919, 1036
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 3 cut(s) 94, 632, 1051
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 2 cut(s) 50, 814
HphI GGTGA 2 cut(s) 820, 983
Hpy166II GTNNAC 3 cut(s) 769, 811, 895
Hpy188I TCNGA 3 cut(s) 360, 426, 631
Hpy188III TCNNGA 4 cut(s) 379, 761, 1033, 1048
Hpy8I GTNNAC 3 cut(s) 769, 811, 895
Hpy99I CGWCG 1 cut(s) 271
HpyAV CCTTC 3 cut(s) 32, 540, 1012
HpyCH4III ACNGT 2 cut(s) 303, 661
HpyCH4IV ACGT 2 cut(s) 206, 1141
HpyCH4V TGCA 8 cut(s) 4, 13, 280, 590, 639, 876, 1062, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 72, 97, 729, 738
HpyF3I CTNAG 5 cut(s) 145, 384, 425, 567, 1023
HpySE526I ACGT 2 cut(s) 206, 1141
Hsp92I GRCGYC 1 cut(s) 323
Hsp92II CATG 8 cut(s) 225, 639, 679, 703, 722, 757, 919, 1036
HspAI GCGC 1 cut(s) 368
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 1 cut(s) 791
LmnI GCTCC 2 cut(s) 763, 1120
Lsp1109I GCAGC 2 cut(s) 87, 1046
LweI GCATC 5 cut(s) 267, 442, 553, 966, 1032
MaeI CTAG 3 cut(s) 63, 327, 525
MaeII ACGT 2 cut(s) 206, 1141
MaeIII GTNAC 2 cut(s) 55, 202
MalI GATC 1 cut(s) 793
MboI GATC 1 cut(s) 791
MboII GAAGA 3 cut(s) 373, 614, 851
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 1 cut(s) 903
MluCI AATT 6 cut(s) 237, 271, 408, 848, 860, 1109
MlyI GAGTC 1 cut(s) 1060
Mph1103I ATGCAT 2 cut(s) 592, 641
MseI TTAA 3 cut(s) 258, 810, 864
MslI CAYNNNNRTG 1 cut(s) 965
MspI CCGG 2 cut(s) 50, 814
MspR9I CCNGG 3 cut(s) 50, 51, 530
MunI CAATTG 1 cut(s) 237
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 1 cut(s) 530
MwoI GCNNNNNNNGC 5 cut(s) 10, 72, 97, 729, 738
NciI CCSGG 2 cut(s) 50, 51
NcoI CCATGG 1 cut(s) 718
NdeII GATC 1 cut(s) 791
NlaIII CATG 8 cut(s) 225, 639, 679, 703, 722, 757, 919, 1036
NlaIV GGNNCC 7 cut(s) 48, 234, 390, 405, 759, 793, 1131
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 2 cut(s) 592, 641
OliI CACNNNNGTG 1 cut(s) 965
PagI TCATGA 1 cut(s) 1032
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 2 cut(s) 94, 632
PflMI CCANNNNNTGG 1 cut(s) 612
PkrI GCNGC 3 cut(s) 102, 264, 1061
PleI GAGTC 1 cut(s) 1059
PpsI GAGTC 1 cut(s) 1059
PpuMI RGGWCCY 2 cut(s) 46, 389
PshAI GACNNNNGTC 1 cut(s) 200
Psp5II RGGWCCY 2 cut(s) 46, 389
Psp6I CCWGG 1 cut(s) 528
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 1 cut(s) 528
PspN4I GGNNCC 7 cut(s) 48, 234, 390, 405, 759, 793, 1131
PspPI GGNCC 4 cut(s) 46, 389, 722, 986
PspPPI RGGWCCY 2 cut(s) 46, 389
PsrI GAACNNNNNNTAC 2 cut(s) 1122, 1154
PstI CTGCAG 1 cut(s) 878
PsuI RGATCY 1 cut(s) 791
RsaI GTAC 3 cut(s) 674, 692, 1123
RsaNI GTAC 3 cut(s) 673, 691, 1122
RseI CAYNNNNRTG 1 cut(s) 965
SaqAI TTAA 3 cut(s) 258, 810, 864
SatI GCNGC 3 cut(s) 101, 263, 1060
Sau3AI GATC 1 cut(s) 791
Sau96I GGNCC 4 cut(s) 46, 389, 722, 986
SchI GAGTC 1 cut(s) 1060
ScrFI CCNGG 3 cut(s) 50, 51, 530
SduI GDGCHC 1 cut(s) 903
SfaNI GCATC 5 cut(s) 267, 442, 553, 966, 1032
SfcI CTRYAG 1 cut(s) 874
SinI GGWCC 3 cut(s) 46, 389, 986
SmaI CCCGGG 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 965
Sse9I AATT 6 cut(s) 237, 271, 408, 848, 860, 1109
SsiI CCGC 3 cut(s) 263, 984, 1039
SspMI CTAG 3 cut(s) 63, 327, 525
StyD4I CCNGG 3 cut(s) 48, 49, 528
StyI CCWWGG 4 cut(s) 81, 326, 718, 1125
TaaI ACNGT 2 cut(s) 303, 661
TaiI ACGT 2 cut(s) 209, 1144
TaqI TCGA 2 cut(s) 269, 837
TasI AATT 6 cut(s) 237, 271, 408, 848, 860, 1109
TatI WGTACW 2 cut(s) 672, 690
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 2 cut(s) 94, 632
Tru1I TTAA 3 cut(s) 258, 810, 864
Tru9I TTAA 3 cut(s) 258, 810, 864
TscAI CASTG 5 cut(s) 153, 664, 785, 803, 1027
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 2 cut(s) 100, 1059
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 8 cut(s) 302, 449, 453, 497, 607, 624, 1021, 1049
TspMI CCCGGG 1 cut(s) 49
TspRI CASTG 5 cut(s) 153, 664, 785, 803, 1027
Van91I CCANNNNNTGG 1 cut(s) 612
VpaK11BI GGWCC 3 cut(s) 46, 389, 986
XapI RAATTY 4 cut(s) 271, 848, 860, 1109
XcmI CCANNNNNNNNNTGG 1 cut(s) 725
XmaI CCCGGG 1 cut(s) 49
XmaJI CCTAGG 1 cut(s) 326
XspI CTAG 3 cut(s) 63, 327, 525
Zsp2I ATGCAT 2 cut(s) 592, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.