MD08G1073800.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005609
Physical Location & Seq
Reverse (-)
5961199 .. 5963868
2670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1073800.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGCAGAACTTGCAATTGAGGGTTGTGCCGGTGGAGGAAGGGAAGGACGCCGGTGTTAGTAGCAGCCGCCCAAGCCAAACGAAGGCCTTGCCGATTAGGCTACTGCAGTTATTCGCGCTGTTTCTGGTTTTCTGTATTACTTTCTCAGTCATTAGCATATACACAATTCGGCATTTTGGAATCATTGGCGTGATGACAACAGTCGCAGCCTCTTTCCAGCCATGCCTTGAGGAACCAAGAGGTTTGGATCGATGGATTAAGCCGCCATCAAGTGTTAAACACACCATGAATGATGAGGAATTGTTGTGGAGGGCTTCATTTACGCCTAGGATAAAGAAATATCCTTTTGAAAGAGTTCCAAAGATTGCATTTATGTTCTTAACTAAGGGGCCGTTGCCGCTGGCACCCCTTTGGGAGAGGTTTCTGAAGGGACACGAAGGGCTTTATTCAGTCTACGTTCATTCACTACCGTCGTTTCAACCCCATTTTAATCCTTCGTCAGTTTTTTATGGGAGACATATTCCAAGCCAGGTTGCTGAGTGGGGAAGAATGAGCATGTGTGATGCCGAGAGAAGACTCCTTGCTAATGCATTGCTCGACATATCCAACGAACGGTTCATTCTTCTTTCTGAATCATGCATTCCGCTCTATAATTTCAGTGTCATTTACCAGTACTTGATGAAGTCCAAGTACAGCTATGTGGGTGCATTTGATGACCCCGGGCCATATGGAAGAGGACGCTACAATGACAACATGGCACCTGAAGTGAGTATATCCAAGTGGCGTAAGGGCTCCCAGTGGTTTGAAGTTAACCGAAAGCTTGCCATCACCATTGTCGAGGATACAAAATTTTACCCCAAATTTAAAGAGTTCTGCAGACCCGCATGTTACGTTGATGAGCACTACTTCCCCACCATGCTAACCATTGAAGCTGGGAATTCTTTAGCAAATAGAAGCATCACTTGGGTAGATTGGTCAAGGGGTGGTCCTCACCCGGCTACCTTTGGAAGAGCGGATATCACCGAGGAATTTTTGAAACGAATTTTTGAGAGACAACGTTGCACTTACAATGACCGGAACTCTACAGTCTGCTTTCTTTTCGGAAGGAAGTTTGCTCCGAGTGCCATGGAACCTCTTTTGCATTTAGCACCAAAGTTTTTGGGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

390

Amino Acids

44.88

Weight (kDa)

9.28

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 122 - 348 7.4e-86 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 403, 757
AccBSI CCGCTC 2 cut(s) 646, 1013
AccI GTMKAC 1 cut(s) 453
AccII CGCG 1 cut(s) 116
AciI CCGC 6 cut(s) 67, 263, 398, 644, 882, 1013
AclI AACGTT 1 cut(s) 1057
AclWI GGATC 1 cut(s) 255
AcsI RAATTY 5 cut(s) 848, 860, 937, 1028, 1041
AcuI CTGAAG 2 cut(s) 446, 783
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 2 cut(s) 674, 692
AfiI CCNNNNNNNGG 2 cut(s) 82, 612
AgsI TTSAA 5 cut(s) 350, 479, 806, 929, 1036
AjnI CCWGG 1 cut(s) 528
AjuI GAANNNNNNNTTGG 4 cut(s) 599, 631, 946, 978
AluBI AGCT 3 cut(s) 696, 820, 932
AluI AGCT 3 cut(s) 696, 820, 932
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 2 cut(s) 508, 1045
AlwI GGATC 1 cut(s) 255
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 3 cut(s) 84, 389, 722
ApeKI GCWGC 2 cut(s) 63, 206
ApoI RAATTY 5 cut(s) 848, 860, 937, 1028, 1041
Asp700I GAANNNNTTC 1 cut(s) 354
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 3 cut(s) 389, 722, 986
AsuC2I CCSGG 3 cut(s) 720, 721, 995
AsuHPI GGTGA 3 cut(s) 820, 983, 1012
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 1 cut(s) 986
AvrII CCTAGG 1 cut(s) 326
BanI GGYRCC 2 cut(s) 403, 757
BanII GRGCYC 1 cut(s) 794
BarI GAAGNNNNNNTAC 2 cut(s) 674, 706
BbsI GAAGAC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 2 cut(s) 75, 218
BccI CCATC 3 cut(s) 246, 274, 833
BceAI ACGGC 1 cut(s) 376
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 530
BciVI GTATCC 1 cut(s) 835
BcnI CCSGG 3 cut(s) 720, 721, 995
BcoDI GTCTC 2 cut(s) 508, 1045
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 3 cut(s) 104, 874, 1083
BfuI GTATCC 1 cut(s) 835
BglI GCCNNNNNGGC 1 cut(s) 97
BisI GCNGC 5 cut(s) 64, 67, 207, 263, 398
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 5 cut(s) 65, 68, 208, 264, 399
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 4 cut(s) 530, 720, 721, 995
Bme18I GGWCC 1 cut(s) 986
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 3 cut(s) 389, 722, 986
BmiI GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
BmrFI CCNGG 4 cut(s) 530, 720, 721, 995
BmrI ACTGGG 1 cut(s) 790
BmsI GCATC 2 cut(s) 553, 966
BmuI ACTGGG 1 cut(s) 790
BoxI GACNNNNGTC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 580
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 3 cut(s) 720, 721, 995
Bsa29I ATCGAT 1 cut(s) 250
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 5 cut(s) 326, 718, 719, 1023, 1125
BsaWI WCCGGW 1 cut(s) 1074
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 612
Bse118I RCCGGY 2 cut(s) 28, 50
Bse1I ACTGG 2 cut(s) 670, 796
Bse3DI GCAATG 1 cut(s) 590
BseBI CCWGG 1 cut(s) 530
BseCI ATCGAT 1 cut(s) 250
BseDI CCNNGG 5 cut(s) 326, 718, 719, 1023, 1125
BseLI CCNNNNNNNGG 2 cut(s) 82, 612
BseMI GCAATG 1 cut(s) 590
BseMII CTCAG 2 cut(s) 159, 528
BseNI ACTGG 2 cut(s) 670, 796
BseXI GCAGC 2 cut(s) 75, 218
BseYI CCCAGC 1 cut(s) 932
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 3 cut(s) 86, 391, 724
BshNI GGYRCC 2 cut(s) 403, 757
BshVI ATCGAT 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 5 cut(s) 29, 51, 720, 995, 1075
BslFI GGGAC 1 cut(s) 444
BslI CCNNNNNNNGG 2 cut(s) 82, 612
BsmAI GTCTC 2 cut(s) 508, 1045
BsmFI GGGAC 1 cut(s) 444
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 3 cut(s) 86, 391, 724
BsoBI CYCGRG 1 cut(s) 719
Bsp1286I GDGCHC 2 cut(s) 794, 903
Bsp143I GATC 1 cut(s) 247
Bsp19I CCATGG 1 cut(s) 1125
BspACI CCGC 6 cut(s) 67, 263, 398, 644, 882, 1013
BspANI GGCC 3 cut(s) 86, 391, 724
BspCNI CTCAG 2 cut(s) 158, 529
BspDI ATCGAT 1 cut(s) 250
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
BspMAI CTGCAG 2 cut(s) 108, 878
BspPI GGATC 1 cut(s) 255
BspQI GCTCTTC 1 cut(s) 1003
BspT107I GGYRCC 2 cut(s) 403, 757
BsrBI CCGCTC 2 cut(s) 646, 1013
BsrDI GCAATG 1 cut(s) 590
BsrFI RCCGGY 2 cut(s) 28, 50
BsrI ACTGG 2 cut(s) 670, 796
BssAI RCCGGY 2 cut(s) 28, 50
BssECI CCNNGG 5 cut(s) 326, 718, 719, 1023, 1125
BssMI GATC 1 cut(s) 247
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 2 cut(s) 326, 1125
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 4 cut(s) 202, 471, 615, 1087
Bst6I CTCTTC 2 cut(s) 727, 1003
BstACI GRCGYC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 402, 822
BstDEI CTNAG 3 cut(s) 145, 384, 537
BstDSI CCRYGG 1 cut(s) 1125
BstFNI CGCG 1 cut(s) 116
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 2 cut(s) 508, 1045
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 5 cut(s) 10, 72, 97, 397, 1121
BstNI CCWGG 1 cut(s) 530
BstNSI RCATGY 2 cut(s) 559, 888
BstPAI GACNNNNGTC 1 cut(s) 200
BstSCI CCNGG 4 cut(s) 528, 718, 719, 993
BstSFI CTRYAG 3 cut(s) 104, 874, 1083
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 2 cut(s) 75, 218
BstV2I GAAGAC 1 cut(s) 580
Bsu15I ATCGAT 1 cut(s) 250
BsuI GTATCC 1 cut(s) 835
BsuRI GGCC 3 cut(s) 86, 391, 724
BsuTUI ATCGAT 1 cut(s) 250
BtgI CCRYGG 1 cut(s) 1125
BtsIMutI CAGTG 2 cut(s) 664, 803
Cac8I GCNNGC 2 cut(s) 402, 822
CfoI GCGC 1 cut(s) 118
Cfr10I RCCGGY 2 cut(s) 28, 50
Cfr13I GGNCC 3 cut(s) 389, 722, 986
Cfr9I CCCGGG 1 cut(s) 719
ClaI ATCGAT 1 cut(s) 250
CseI GACGC 2 cut(s) 56, 747
Csp6I GTAC 2 cut(s) 673, 691
CviAII CATG 8 cut(s) 222, 286, 556, 636, 754, 885, 916, 1126
CviQI GTAC 2 cut(s) 673, 691
DdeI CTNAG 3 cut(s) 145, 384, 537
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
DraI TTTAAA 1 cut(s) 865
Eam1104I CTCTTC 2 cut(s) 727, 1003
EarI CTCTTC 2 cut(s) 727, 1003
Eco130I CCWWGG 2 cut(s) 326, 1125
Eco147I AGGCCT 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 794
Eco32I GATATC 1 cut(s) 1018
Eco47I GGWCC 1 cut(s) 986
Eco57I CTGAAG 2 cut(s) 446, 783
Eco88I CYCGRG 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 937
EcoRII CCWGG 1 cut(s) 528
EcoRV GATATC 1 cut(s) 1018
EcoT14I CCWWGG 2 cut(s) 326, 1125
EcoT22I ATGCAT 2 cut(s) 592, 641
EcoT38I GRGCYC 1 cut(s) 794
ErhI CCWWGG 2 cut(s) 326, 1125
FaeI CATG 8 cut(s) 225, 289, 559, 639, 757, 888, 919, 1129
FalI AAGNNNNNCTT 2 cut(s) 946, 978
FaqI GGGAC 1 cut(s) 444
FatI CATG 8 cut(s) 221, 285, 555, 635, 753, 884, 915, 1125
FauI CCCGC 1 cut(s) 889
FauNDI CATATG 1 cut(s) 727
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 5 cut(s) 64, 67, 207, 263, 398
FriOI GRGCYC 1 cut(s) 794
Fsp4HI GCNGC 5 cut(s) 64, 67, 207, 263, 398
FspBI CTAG 1 cut(s) 327
GlaI GCGC 1 cut(s) 117
GluI GCNGC 5 cut(s) 64, 67, 207, 263, 398
GsaI CCCAGC 1 cut(s) 936
HaeIII GGCC 3 cut(s) 86, 391, 724
HapII CCGG 5 cut(s) 29, 51, 720, 995, 1075
HgaI GACGC 2 cut(s) 56, 747
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 8 cut(s) 225, 289, 559, 639, 757, 888, 919, 1129
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 3 cut(s) 180, 576, 632
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 5 cut(s) 29, 51, 720, 995, 1075
HphI GGTGA 3 cut(s) 820, 983, 1012
Hpy166II GTNNAC 2 cut(s) 454, 811
Hpy188I TCNGA 4 cut(s) 426, 631, 1103, 1119
Hpy8I GTNNAC 2 cut(s) 454, 811
Hpy99I CGWCG 1 cut(s) 475
HpyAV CCTTC 7 cut(s) 32, 37, 76, 421, 431, 504, 1098
HpyCH4III ACNGT 4 cut(s) 202, 471, 615, 1087
HpyCH4IV ACGT 3 cut(s) 456, 891, 1057
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 72, 97, 397, 1121
HpyF3I CTNAG 3 cut(s) 145, 384, 537
HpySE526I ACGT 3 cut(s) 456, 891, 1057
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 8 cut(s) 225, 289, 559, 639, 757, 888, 919, 1129
HspAI GCGC 1 cut(s) 116
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 1 cut(s) 247
LguI GCTCTTC 1 cut(s) 1003
LmnI GCTCC 2 cut(s) 797, 1120
Lsp1109I GCAGC 2 cut(s) 75, 218
LweI GCATC 2 cut(s) 553, 966
MaeI CTAG 1 cut(s) 327
MaeII ACGT 3 cut(s) 456, 891, 1057
MaeIII GTNAC 1 cut(s) 887
MalI GATC 1 cut(s) 249
MbiI CCGCTC 2 cut(s) 646, 1013
MboI GATC 1 cut(s) 247
MboII GAAGA 5 cut(s) 558, 585, 614, 744, 1020
MfeI CAATTG 1 cut(s) 14
MhlI GDGCHC 2 cut(s) 794, 903
MluCI AATT 9 cut(s) 14, 165, 299, 652, 848, 860, 937, 1028, 1041
MlyI GAGTC 1 cut(s) 570
MmeI TCCRAC 1 cut(s) 630
Mph1103I ATGCAT 2 cut(s) 592, 641
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 6 cut(s) 258, 276, 380, 489, 810, 864
MslI CAYNNNNRTG 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 400
MspI CCGG 5 cut(s) 29, 51, 720, 995, 1075
MspR9I CCNGG 4 cut(s) 530, 720, 721, 995
MunI CAATTG 1 cut(s) 14
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 1 cut(s) 530
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 5 cut(s) 10, 72, 97, 397, 1121
NciI CCSGG 3 cut(s) 720, 721, 995
NcoI CCATGG 1 cut(s) 1125
NdeI CATATG 1 cut(s) 727
NdeII GATC 1 cut(s) 247
NlaIII CATG 8 cut(s) 225, 289, 559, 639, 757, 888, 919, 1129
NlaIV GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
NmeAIII GCCGAG 1 cut(s) 592
NsiI ATGCAT 2 cut(s) 592, 641
NspI RCATGY 2 cut(s) 559, 888
PceI AGGCCT 1 cut(s) 86
PciSI GCTCTTC 1 cut(s) 1003
PctI GAATGC 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 2 cut(s) 180, 632
PkrI GCNGC 5 cut(s) 65, 68, 208, 264, 399
PleI GAGTC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 570
PshAI GACNNNNGTC 1 cut(s) 200
Psp1406I AACGTT 1 cut(s) 1057
Psp6I CCWGG 1 cut(s) 528
PspFI CCCAGC 1 cut(s) 932
PspGI CCWGG 1 cut(s) 528
PspN4I GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
PspPI GGNCC 3 cut(s) 389, 722, 986
PstI CTGCAG 2 cut(s) 108, 878
RsaI GTAC 2 cut(s) 674, 692
RsaNI GTAC 2 cut(s) 673, 691
RseI CAYNNNNRTG 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 1003
SaqAI TTAA 6 cut(s) 258, 276, 380, 489, 810, 864
SatI GCNGC 5 cut(s) 64, 67, 207, 263, 398
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 3 cut(s) 389, 722, 986
ScaI AGTACT 1 cut(s) 674
SchI GAGTC 1 cut(s) 570
ScrFI CCNGG 4 cut(s) 530, 720, 721, 995
SduI GDGCHC 2 cut(s) 794, 903
SfaNI GCATC 2 cut(s) 553, 966
SfcI CTRYAG 3 cut(s) 104, 874, 1083
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 1 cut(s) 986
SmaI CCCGGG 1 cut(s) 721
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 9 cut(s) 14, 165, 299, 652, 848, 860, 937, 1028, 1041
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 6 cut(s) 67, 263, 398, 644, 882, 1013
SspMI CTAG 1 cut(s) 327
StuI AGGCCT 1 cut(s) 86
StyD4I CCNGG 4 cut(s) 528, 718, 719, 993
StyI CCWWGG 2 cut(s) 326, 1125
TaaI ACNGT 4 cut(s) 202, 471, 615, 1087
TaiI ACGT 3 cut(s) 459, 894, 1060
TaqI TCGA 3 cut(s) 250, 597, 837
TasI AATT 9 cut(s) 14, 165, 299, 652, 848, 860, 937, 1028, 1041
TatI WGTACW 2 cut(s) 672, 690
TauI GCSGC 3 cut(s) 69, 265, 400
TfiI GAWTC 2 cut(s) 180, 632
Tru1I TTAA 6 cut(s) 258, 276, 380, 489, 810, 864
Tru9I TTAA 6 cut(s) 258, 276, 380, 489, 810, 864
TscAI CASTG 2 cut(s) 664, 803
TseI GCWGC 2 cut(s) 63, 206
TspDTI ATGAA 5 cut(s) 302, 306, 449, 607, 695
TspMI CCCGGG 1 cut(s) 719
TspRI CASTG 2 cut(s) 664, 803
VpaK11BI GGWCC 1 cut(s) 986
XapI RAATTY 5 cut(s) 848, 860, 937, 1028, 1041
XceI RCATGY 2 cut(s) 559, 888
XmaI CCCGGG 1 cut(s) 719
XmaJI CCTAGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 1 cut(s) 327
ZrmI AGTACT 1 cut(s) 674
Zsp2I ATGCAT 2 cut(s) 592, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.