Rw6G043010

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005609
Physical Location & Seq
Forward (+)
66413979 .. 66417307
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043010.1

Sequence Viewer

Length: 1164 bp
ATGCAATCAAGGGTTGTGCCATTAGAGGAAGGCAAGGACCCGGGAGTTAGTAGTAGCAGGACGAACCAAACCAAGGCGTTGTTGCCGATTCGGATGCTTCAGTTATTTGTGCTGTTTCTGGCTTTCTGTATCACTTTCTCAGTGATTAGTATATACACCATTCGGCATTTTGGGATTAGTAACGTGATGACTTCAGTCACGTCCTCTGTCCAGCCATGTTTTGAGGAACCAACTGGTTTGGATAGATGGATTAAGCCGCCATCGAATTTGATGCACACTATGAATGACGACGAACTGTTGTGGAGGGCTTCTTTGTCGTCTAGGAGAAAGAAGTATCCTTTTGAGAGAGTTCCGAAGATTGCGCTTATGTTCTTGACTAAGGGGCCTTTGCCTCTGGCACCAATTTGGGAGAGGTTTCTGAAGGGGCATGAAGGATTTTATTCAGTCTATGTTCATTCACTGCCATCATTTCAGCCTCATTTTCCACCTTCCTCGGTTTTTCATGGGAGACATATTCCTAGCCAGGTTGCTGAATGGGGAAGGATGAGTATGTGTGATGCTGAGAGGAGACTTCTTGCTAATGCATTGCTTGACATATCCAACGAATGGTTCATTCTTCTTTCTGAATCATGCATTCCTCTCTATAACTTCAGCGTCATTTACCAGTACATGAGGAAGTCCAAGTACAGCTACATGGGTGCATTTGATGACCACGGCCCGTATGGCAGAGGACGCTACAATGATAACATGGCTCCTGAAGTGAAGATTACCCAGTGGCGTAAGGGATCCCAGTGGTTTGAAGTTAACCGGAAGCTTGCCATCGCCATCGTTGAAGATACAACATTTTTCCCAAAATTCAAAGAGTTCTGCAGACCAGCATGTTATGTTGATGAGCACTACTTCCCCACCATGCTAACCATTCAAGCAGGGAATATGCTAGCAAACAGAAGCATCACTTGGGTGGATTGGTCAAGGGGCGGACCTCACCCGGCCACCTTTGGCAGAGGAGATATCACCGAGGAATTCATGAAGCGGATGCTCTCAAGTCATCGTTGCACTTACAACAACCGAAACTCTACAATGTGCTTTCTATTTGCAAGAAAATTTGCTCCCAGTGCCTTGGAACCTCTCTTACAGTTAGCACCCAAGTTTTTGGGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

387

Amino Acids

44.74

Weight (kDa)

9.32

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 120 - 346 1.2e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 397
AccB7I CCANNNNNTGG 1 cut(s) 606
AciI CCGC 3 cut(s) 257, 978, 1033
AclWI GGATC 2 cut(s) 780, 793
AcoI YGGCCR 1 cut(s) 990
AcsI RAATTY 4 cut(s) 265, 854, 1022, 1103
AcuI CTGAAG 5 cut(s) 83, 177, 440, 634, 777
AfaI GTAC 2 cut(s) 668, 686
AfiI CCNNNNNNNGG 2 cut(s) 73, 606
AgsI TTSAA 4 cut(s) 800, 833, 859, 923
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 1 cut(s) 522
AjuI GAANNNNNNNTTGG 4 cut(s) 593, 625, 940, 972
AleI CACNNNNGTG 1 cut(s) 959
AluBI AGCT 2 cut(s) 690, 814
AluI AGCT 2 cut(s) 690, 814
Alw21I GWGCWC 1 cut(s) 897
Alw26I GTCTC 2 cut(s) 502, 562
AlwI GGATC 2 cut(s) 780, 793
Ama87I CYCGRG 1 cut(s) 40
AoxI GGCC 3 cut(s) 383, 715, 990
ApoI RAATTY 4 cut(s) 265, 854, 1022, 1103
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 4 cut(s) 37, 383, 716, 980
AsuC2I CCSGG 3 cut(s) 41, 42, 989
AsuHPI GGTGA 2 cut(s) 977, 1006
AsuNHI GCTAGC 1 cut(s) 937
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 37, 980
BamHI GGATCC 1 cut(s) 785
BanI GGYRCC 1 cut(s) 397
BarI GAAGNNNNNNTAC 2 cut(s) 668, 700
Bbv12I GWGCWC 1 cut(s) 897
BccI CCATC 5 cut(s) 240, 268, 472, 827, 833
BceAI ACGGC 1 cut(s) 730
BcgI CGANNNNNNTGC 4 cut(s) 76, 110, 253, 287
BciT130I CCWGG 1 cut(s) 524
BciVI GTATCC 1 cut(s) 345
BcnI CCSGG 3 cut(s) 41, 42, 989
BcoDI GTCTC 2 cut(s) 502, 562
BfaI CTAG 3 cut(s) 321, 519, 938
BfmI CTRYAG 1 cut(s) 868
BfuI GTATCC 1 cut(s) 345
BglI GCCNNNNNGGC 1 cut(s) 723
BisI GCNGC 1 cut(s) 257
BlsI GCNGC 1 cut(s) 258
Bme1390I CCNGG 4 cut(s) 41, 42, 524, 989
Bme18I GGWCC 2 cut(s) 37, 980
BmeT110I CYCGRG 1 cut(s) 40
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 4 cut(s) 37, 383, 716, 980
BmiI GGNNCC 7 cut(s) 39, 228, 384, 399, 753, 787, 1125
BmrFI CCNGG 4 cut(s) 41, 42, 524, 989
BmrI ACTGGG 3 cut(s) 766, 784, 1107
BmsI GCATC 5 cut(s) 84, 261, 547, 960, 1026
BmtI GCTAGC 1 cut(s) 941
BmuI ACTGGG 3 cut(s) 766, 784, 1107
BoxI GACNNNNGTC 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 1027
BpuMI CCSGG 3 cut(s) 41, 42, 989
BsaJI CCNNGG 6 cut(s) 40, 72, 492, 712, 1017, 1119
BsaWI WCCGGW 1 cut(s) 807
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 606
Bse1I ACTGG 5 cut(s) 238, 664, 772, 790, 1113
Bse3DI GCAATG 1 cut(s) 584
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 6 cut(s) 40, 72, 492, 712, 1017, 1119
BseGI GGATG 3 cut(s) 99, 549, 1041
BseLI CCNNNNNNNGG 2 cut(s) 73, 606
BseMI GCAATG 1 cut(s) 584
BseMII CTCAG 2 cut(s) 153, 552
BseNI ACTGG 5 cut(s) 238, 664, 772, 790, 1113
BseRI GAGGAG 2 cut(s) 580, 1020
BshFI GGCC 3 cut(s) 385, 717, 992
BshNI GGYRCC 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 897
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 3 cut(s) 41, 808, 989
BslI CCNNNNNNNGG 2 cut(s) 73, 606
BsmAI GTCTC 2 cut(s) 502, 562
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 3 cut(s) 385, 717, 992
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 897
Bsp143I GATC 1 cut(s) 785
BspACI CCGC 3 cut(s) 257, 978, 1033
BspANI GGCC 3 cut(s) 385, 717, 992
BspCNI CTCAG 2 cut(s) 152, 553
BspHI TCATGA 1 cut(s) 1026
BspLI GGNNCC 7 cut(s) 39, 228, 384, 399, 753, 787, 1125
BspMAI CTGCAG 1 cut(s) 872
BspOI GCTAGC 1 cut(s) 941
BspPI GGATC 2 cut(s) 780, 793
BspT107I GGYRCC 1 cut(s) 397
BsrDI GCAATG 1 cut(s) 584
BsrI ACTGG 5 cut(s) 238, 664, 772, 790, 1113
BssECI CCNNGG 6 cut(s) 40, 72, 492, 712, 1017, 1119
BssMI GATC 1 cut(s) 785
BssT1I CCWWGG 2 cut(s) 72, 1119
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 2 cut(s) 297, 1137
BstC8I GCNNGC 2 cut(s) 816, 939
BstDEI CTNAG 3 cut(s) 139, 378, 561
BstDSI CCRYGG 1 cut(s) 712
BstF5I GGATG 3 cut(s) 99, 549, 1041
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 1 cut(s) 788
BstMAI GTCTC 2 cut(s) 502, 562
BstMBI GATC 1 cut(s) 785
BstMWI GCNNNNNNNGC 3 cut(s) 723, 732, 1115
BstNI CCWGG 1 cut(s) 524
BstNSI RCATGY 1 cut(s) 882
BstPAI GACNNNNGTC 1 cut(s) 194
BstSCI CCNGG 4 cut(s) 39, 40, 522, 987
BstSFI CTRYAG 1 cut(s) 868
BstX2I RGATCY 1 cut(s) 785
BstXI CCANNNNNNTGG 2 cut(s) 1120, 1153
BstYI RGATCY 1 cut(s) 785
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 3 cut(s) 385, 717, 992
BtgI CCRYGG 1 cut(s) 712
BtgZI GCGATG 1 cut(s) 805
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 3 cut(s) 99, 549, 1041
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 5 cut(s) 147, 458, 779, 797, 1120
Cac8I GCNNGC 2 cut(s) 816, 939
CciI TCATGA 1 cut(s) 1026
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 4 cut(s) 37, 383, 716, 980
Cfr9I CCCGGG 1 cut(s) 40
CseI GACGC 2 cut(s) 643, 741
Csp6I GTAC 2 cut(s) 667, 685
CviQI GTAC 2 cut(s) 667, 685
DdeI CTNAG 3 cut(s) 139, 378, 561
DpnI GATC 1 cut(s) 787
DpnII GATC 1 cut(s) 785
EaeI YGGCCR 1 cut(s) 990
EciI GGCGGA 1 cut(s) 993
Eco130I CCWWGG 2 cut(s) 72, 1119
Eco32I GATATC 1 cut(s) 1012
Eco47I GGWCC 2 cut(s) 37, 980
Eco57I CTGAAG 5 cut(s) 83, 177, 440, 634, 777
Eco88I CYCGRG 1 cut(s) 40
EcoO109I RGGNCCY 2 cut(s) 37, 383
EcoRI GAATTC 1 cut(s) 1022
EcoRII CCWGG 1 cut(s) 522
EcoRV GATATC 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 72, 1119
EcoT22I ATGCAT 2 cut(s) 586, 635
ErhI CCWWGG 2 cut(s) 72, 1119
FalI AAGNNNNNCTT 2 cut(s) 940, 972
Fnu4HI GCNGC 1 cut(s) 257
FokI GGATG 3 cut(s) 106, 556, 1048
Fsp4HI GCNGC 1 cut(s) 257
FspBI CTAG 3 cut(s) 321, 519, 938
GlaI GCGC 1 cut(s) 363
GluI GCNGC 1 cut(s) 257
HaeIII GGCC 3 cut(s) 385, 717, 992
HapII CCGG 3 cut(s) 41, 808, 989
HgaI GACGC 2 cut(s) 643, 741
HhaI GCGC 1 cut(s) 364
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 1 cut(s) 812
HinfI GANTC 3 cut(s) 88, 626, 1158
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 3 cut(s) 41, 808, 989
HphI GGTGA 2 cut(s) 977, 1006
Hpy166II GTNNAC 1 cut(s) 805
Hpy188I TCNGA 5 cut(s) 93, 354, 420, 625, 1163
Hpy188III TCNNGA 3 cut(s) 373, 755, 1027
Hpy8I GTNNAC 1 cut(s) 805
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 5 cut(s) 23, 415, 425, 498, 534
HpyCH4III ACNGT 2 cut(s) 297, 1137
HpyCH4IV ACGT 2 cut(s) 183, 200
HpyCH4V TGCA 8 cut(s) 4, 274, 584, 633, 701, 870, 1056, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 723, 732, 1115
HpyF3I CTNAG 3 cut(s) 139, 378, 561
HpySE526I ACGT 2 cut(s) 183, 200
HspAI GCGC 1 cut(s) 362
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 1 cut(s) 785
LmnI GCTCC 2 cut(s) 757, 1114
LweI GCATC 5 cut(s) 84, 261, 547, 960, 1026
MaeI CTAG 3 cut(s) 321, 519, 938
MaeII ACGT 2 cut(s) 183, 200
MaeIII GTNAC 2 cut(s) 179, 196
MalI GATC 1 cut(s) 787
MboI GATC 1 cut(s) 785
MboII GAAGA 4 cut(s) 367, 608, 775, 845
MflI RGATCY 1 cut(s) 785
MhlI GDGCHC 1 cut(s) 897
MluCI AATT 5 cut(s) 265, 402, 854, 1022, 1103
MmeI TCCRAC 1 cut(s) 624
Mph1103I ATGCAT 2 cut(s) 586, 635
MseI TTAA 2 cut(s) 252, 804
MslI CAYNNNNRTG 1 cut(s) 959
MspI CCGG 3 cut(s) 41, 808, 989
MspR9I CCNGG 4 cut(s) 41, 42, 524, 989
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 723, 732, 1115
NciI CCSGG 3 cut(s) 41, 42, 989
NdeII GATC 1 cut(s) 785
NheI GCTAGC 1 cut(s) 937
NlaIV GGNNCC 7 cut(s) 39, 228, 384, 399, 753, 787, 1125
NmuCI GTSAC 1 cut(s) 196
NsiI ATGCAT 2 cut(s) 586, 635
NspI RCATGY 1 cut(s) 882
OliI CACNNNNGTG 1 cut(s) 959
PagI TCATGA 1 cut(s) 1026
PctI GAATGC 1 cut(s) 633
PfeI GAWTC 3 cut(s) 88, 626, 1158
PflMI CCANNNNNTGG 1 cut(s) 606
PkrI GCNGC 1 cut(s) 258
PpuMI RGGWCCY 1 cut(s) 37
PshAI GACNNNNGTC 1 cut(s) 194
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 7 cut(s) 39, 228, 384, 399, 753, 787, 1125
PspPI GGNCC 4 cut(s) 37, 383, 716, 980
PspPPI RGGWCCY 1 cut(s) 37
PsrI GAACNNNNNNTAC 2 cut(s) 1116, 1148
PstI CTGCAG 1 cut(s) 872
PsuI RGATCY 1 cut(s) 785
RsaI GTAC 2 cut(s) 668, 686
RsaNI GTAC 2 cut(s) 667, 685
RseI CAYNNNNRTG 1 cut(s) 959
SaqAI TTAA 2 cut(s) 252, 804
SatI GCNGC 1 cut(s) 257
Sau3AI GATC 1 cut(s) 785
Sau96I GGNCC 4 cut(s) 37, 383, 716, 980
ScrFI CCNGG 4 cut(s) 41, 42, 524, 989
SduI GDGCHC 1 cut(s) 897
SfaNI GCATC 5 cut(s) 84, 261, 547, 960, 1026
SfcI CTRYAG 1 cut(s) 868
SinI GGWCC 2 cut(s) 37, 980
SmaI CCCGGG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 959
SmlI CTYRAG 1 cut(s) 1042
SmoI CTYRAG 1 cut(s) 1042
Sse9I AATT 5 cut(s) 265, 402, 854, 1022, 1103
SsiI CCGC 3 cut(s) 257, 978, 1033
SspMI CTAG 3 cut(s) 321, 519, 938
StyD4I CCNGG 4 cut(s) 39, 40, 522, 987
StyI CCWWGG 2 cut(s) 72, 1119
TaaI ACNGT 2 cut(s) 297, 1137
TaiI ACGT 2 cut(s) 186, 203
TaqI TCGA 1 cut(s) 263
TasI AATT 5 cut(s) 265, 402, 854, 1022, 1103
TatI WGTACW 2 cut(s) 666, 684
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 3 cut(s) 88, 626, 1158
Tru1I TTAA 2 cut(s) 252, 804
Tru9I TTAA 2 cut(s) 252, 804
TscAI CASTG 5 cut(s) 147, 465, 779, 797, 1120
TseFI GTSAC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 7 cut(s) 296, 443, 444, 491, 601, 1015, 1043
TspMI CCCGGG 1 cut(s) 40
TspRI CASTG 5 cut(s) 147, 465, 779, 797, 1120
Van91I CCANNNNNTGG 1 cut(s) 606
VpaK11BI GGWCC 2 cut(s) 37, 980
XapI RAATTY 4 cut(s) 265, 854, 1022, 1103
XceI RCATGY 1 cut(s) 882
XcmI CCANNNNNNNNNTGG 1 cut(s) 719
XmaI CCCGGG 1 cut(s) 40
XspI CTAG 3 cut(s) 321, 519, 938
Zsp2I ATGCAT 2 cut(s) 586, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.