RchiOBHm_Chr6g0310441

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005609
Physical Location & Seq
Forward (+)
67788131 .. 67791549
3419 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27917

Sequence Viewer

Length: 1071 bp
ATGCTTCAGTTATTTGTGCTGTTTCTGGCTTTCTGTATCACTTTCTCAGTGATTAGTATATACACCATTCGGCATTTTGGGATTAGTAACGTGATGACTTCAGTCACGTCCTCTGTCCAGCCATGTTTTGAGGAACCAACTGGTTTGGATAGATGGATTAAGCCGCCATCGAATTTGATGCACACTATGAATGACGACGAACTGTTGTGGAGGGCTTCTTTGTCGTCTAGGAGAAAGAAGTATCCTTTTGAGAGAGTTCCGAAGATTGCGCTTATGTTCTTGACTAAGGGGCCTTTGCCTCTGGCACCAATTTGGGAGAGGTTTCTGAAGGGGCATGAAGGATTTTATTCAGTCTATGTTCATTCACTGCCATCATTTCAGCCTCATTTTCCACCTTCCTCGGTTTTTCATGGGAGACATATTCCTAGCCAGGTTGCCGAATGGGGAAGGATGAGTATGTGTGATGCTGAGAGGAGACTTCTTGCTAATGCATTGCTTGACATATCCAACGAATGGTTCATTCTTCTTTCTGAATCATGCATTCCTCTCTATAACTTCAGCGTCATTTACCAGTACATGAGGAAGTCCAAGCACAGCTACATGGGTGCGTTTGATGACCACGGCCCGTATGGCAGAGGACGCTACAATGATAACATGGCTCCTGAAGTGAAGATTACCCAGTGGCGTAAGGGATCCCAGTGGTTTGAAGTTAACCGGAAGCTTGCCGTCGCCATCGTTGAAGATACAACATTTTACCCAAAATTCAAAGAGTTCTGCAGACCAGCATGTTATGTTGATGAGCACTACTTCCCCACCATGCTAACCATTCAAGCAGGGAATATGCTAGCAAACAGAAGCATCACTTGGGTGGATTGGTCAAGGGGCGGACCTCACCCGGCCACCTTTGGCAGAGGAGATATCACCGAGGAATTCATGAAGCGGATGCTCTCAAGTCATCGTTGCACTTACAACAACCGAAACTCTACAATGTGCTTTCTATTTGCAAGAAAATTTGCTCCCAGTGCCTTGGAACCTCTCTTACAGTTAGCACCAAAGTTTTTGGGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

356

Amino Acids

41.34

Weight (kDa)

9.2

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 89 - 315 1.5e-83 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 304
AccB7I CCANNNNNTGG 1 cut(s) 513
AciI CCGC 3 cut(s) 164, 885, 940
AclWI GGATC 2 cut(s) 687, 700
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 4 cut(s) 172, 761, 929, 1010
AcuI CTGAAG 4 cut(s) 84, 347, 541, 684
AfaI GTAC 1 cut(s) 575
AfiI CCNNNNNNNGG 1 cut(s) 513
AgsI TTSAA 4 cut(s) 707, 740, 766, 830
AjiI CACGTC 1 cut(s) 108
AjnI CCWGG 1 cut(s) 429
AjuI GAANNNNNNNTTGG 4 cut(s) 500, 532, 847, 879
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 2 cut(s) 597, 721
AluI AGCT 2 cut(s) 597, 721
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 2 cut(s) 409, 469
AlwI GGATC 2 cut(s) 687, 700
AoxI GGCC 3 cut(s) 290, 622, 897
ApoI RAATTY 4 cut(s) 172, 761, 929, 1010
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 290, 623, 887
AsuC2I CCSGG 1 cut(s) 896
AsuHPI GGTGA 2 cut(s) 884, 913
AsuNHI GCTAGC 1 cut(s) 844
AvaII GGWCC 1 cut(s) 887
BamHI GGATCC 1 cut(s) 692
BanI GGYRCC 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 804
BccI CCATC 4 cut(s) 147, 175, 379, 740
BceAI ACGGC 2 cut(s) 637, 710
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 1 cut(s) 431
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 896
BcoDI GTCTC 2 cut(s) 409, 469
BfaI CTAG 3 cut(s) 228, 426, 845
BfmI CTRYAG 1 cut(s) 775
BfuI GTATCC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 630
BisI GCNGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 165
Bme1390I CCNGG 2 cut(s) 431, 896
Bme18I GGWCC 1 cut(s) 887
BmgBI CACGTC 1 cut(s) 108
BmgT120I GGNCC 3 cut(s) 290, 623, 887
BmiI GGNNCC 6 cut(s) 135, 291, 306, 660, 694, 1032
BmrFI CCNGG 2 cut(s) 431, 896
BmrI ACTGGG 3 cut(s) 673, 691, 1014
BmsI GCATC 4 cut(s) 168, 454, 867, 933
BmtI GCTAGC 1 cut(s) 848
BmuI ACTGGG 3 cut(s) 673, 691, 1014
BoxI GACNNNNGTC 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 934
BpuMI CCSGG 1 cut(s) 896
BsaJI CCNNGG 4 cut(s) 399, 619, 924, 1026
BsaWI WCCGGW 1 cut(s) 714
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse1I ACTGG 5 cut(s) 145, 571, 679, 697, 1020
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 4 cut(s) 399, 619, 924, 1026
BseGI GGATG 2 cut(s) 456, 948
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 2 cut(s) 60, 459
BseNI ACTGG 5 cut(s) 145, 571, 679, 697, 1020
BseRI GAGGAG 2 cut(s) 487, 927
BshFI GGCC 3 cut(s) 292, 624, 899
BshNI GGYRCC 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 804
BsiSI CCGG 2 cut(s) 715, 896
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 2 cut(s) 409, 469
BsmI GAATGC 1 cut(s) 540
BsnI GGCC 3 cut(s) 292, 624, 899
Bsp1286I GDGCHC 1 cut(s) 804
Bsp143I GATC 1 cut(s) 692
BspACI CCGC 3 cut(s) 164, 885, 940
BspANI GGCC 3 cut(s) 292, 624, 899
BspCNI CTCAG 2 cut(s) 59, 460
BspHI TCATGA 1 cut(s) 933
BspLI GGNNCC 6 cut(s) 135, 291, 306, 660, 694, 1032
BspMAI CTGCAG 1 cut(s) 779
BspOI GCTAGC 1 cut(s) 848
BspPI GGATC 2 cut(s) 687, 700
BspT107I GGYRCC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 491
BsrI ACTGG 5 cut(s) 145, 571, 679, 697, 1020
BssECI CCNNGG 4 cut(s) 399, 619, 924, 1026
BssMI GATC 1 cut(s) 692
BssT1I CCWWGG 1 cut(s) 1026
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 2 cut(s) 204, 1044
BstC8I GCNNGC 2 cut(s) 723, 846
BstDEI CTNAG 3 cut(s) 46, 285, 468
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 2 cut(s) 456, 948
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 1 cut(s) 695
BstMAI GTCTC 2 cut(s) 409, 469
BstMBI GATC 1 cut(s) 692
BstMWI GCNNNNNNNGC 3 cut(s) 630, 639, 1022
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 789
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 429, 894
BstSFI CTRYAG 1 cut(s) 775
BstX2I RGATCY 1 cut(s) 692
BstXI CCANNNNNNTGG 1 cut(s) 1027
BstYI RGATCY 1 cut(s) 692
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 292, 624, 899
BtgI CCRYGG 1 cut(s) 619
BtrI CACGTC 1 cut(s) 108
BtsCI GGATG 2 cut(s) 456, 948
BtsI GCAGTG 1 cut(s) 365
BtsIMutI CAGTG 5 cut(s) 54, 365, 686, 704, 1027
Cac8I GCNNGC 2 cut(s) 723, 846
CciI TCATGA 1 cut(s) 933
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 3 cut(s) 290, 623, 887
CseI GACGC 2 cut(s) 550, 648
Csp6I GTAC 1 cut(s) 574
CviQI GTAC 1 cut(s) 574
DdeI CTNAG 3 cut(s) 46, 285, 468
DpnI GATC 1 cut(s) 694
DpnII GATC 1 cut(s) 692
EaeI YGGCCR 1 cut(s) 897
EciI GGCGGA 1 cut(s) 900
Eco130I CCWWGG 1 cut(s) 1026
Eco32I GATATC 1 cut(s) 919
Eco47I GGWCC 1 cut(s) 887
Eco57I CTGAAG 4 cut(s) 84, 347, 541, 684
EcoO109I RGGNCCY 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 429
EcoRV GATATC 1 cut(s) 919
EcoT14I CCWWGG 1 cut(s) 1026
EcoT22I ATGCAT 2 cut(s) 493, 542
ErhI CCWWGG 1 cut(s) 1026
FalI AAGNNNNNCTT 2 cut(s) 847, 879
Fnu4HI GCNGC 1 cut(s) 164
FokI GGATG 2 cut(s) 463, 955
Fsp4HI GCNGC 1 cut(s) 164
FspBI CTAG 3 cut(s) 228, 426, 845
GlaI GCGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 164
HaeIII GGCC 3 cut(s) 292, 624, 899
HapII CCGG 2 cut(s) 715, 896
HgaI GACGC 2 cut(s) 550, 648
HhaI GCGC 1 cut(s) 271
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 2 cut(s) 533, 1065
HpaI GTTAAC 1 cut(s) 712
HpaII CCGG 2 cut(s) 715, 896
HphI GGTGA 2 cut(s) 884, 913
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 4 cut(s) 261, 327, 532, 1070
Hpy188III TCNNGA 3 cut(s) 280, 662, 934
Hpy8I GTNNAC 1 cut(s) 712
Hpy99I CGWCG 2 cut(s) 200, 731
HpyAV CCTTC 4 cut(s) 322, 332, 405, 441
HpyCH4III ACNGT 2 cut(s) 204, 1044
HpyCH4IV ACGT 2 cut(s) 90, 107
HpyCH4V TGCA 6 cut(s) 181, 491, 540, 777, 963, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 630, 639, 1022
HpyF3I CTNAG 3 cut(s) 46, 285, 468
HpySE526I ACGT 2 cut(s) 90, 107
HspAI GCGC 1 cut(s) 269
KspAI GTTAAC 1 cut(s) 712
Kzo9I GATC 1 cut(s) 692
LmnI GCTCC 2 cut(s) 664, 1021
LweI GCATC 4 cut(s) 168, 454, 867, 933
MaeI CTAG 3 cut(s) 228, 426, 845
MaeII ACGT 2 cut(s) 90, 107
MaeIII GTNAC 2 cut(s) 86, 103
MalI GATC 1 cut(s) 694
MboI GATC 1 cut(s) 692
MboII GAAGA 4 cut(s) 274, 515, 682, 752
MflI RGATCY 1 cut(s) 692
MhlI GDGCHC 1 cut(s) 804
MluCI AATT 5 cut(s) 172, 309, 761, 929, 1010
MmeI TCCRAC 1 cut(s) 531
Mph1103I ATGCAT 2 cut(s) 493, 542
MseI TTAA 2 cut(s) 159, 711
MslI CAYNNNNRTG 1 cut(s) 866
MspI CCGG 2 cut(s) 715, 896
MspR9I CCNGG 2 cut(s) 431, 896
Mva1269I GAATGC 1 cut(s) 540
MvaI CCWGG 1 cut(s) 431
MwoI GCNNNNNNNGC 3 cut(s) 630, 639, 1022
NciI CCSGG 1 cut(s) 896
NdeII GATC 1 cut(s) 692
NheI GCTAGC 1 cut(s) 844
NlaIV GGNNCC 6 cut(s) 135, 291, 306, 660, 694, 1032
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 2 cut(s) 493, 542
NspI RCATGY 1 cut(s) 789
OliI CACNNNNGTG 1 cut(s) 866
PagI TCATGA 1 cut(s) 933
PctI GAATGC 1 cut(s) 540
PfeI GAWTC 2 cut(s) 533, 1065
PflMI CCANNNNNTGG 1 cut(s) 513
PkrI GCNGC 1 cut(s) 165
PshAI GACNNNNGTC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 6 cut(s) 135, 291, 306, 660, 694, 1032
PspPI GGNCC 3 cut(s) 290, 623, 887
PsrI GAACNNNNNNTAC 2 cut(s) 1023, 1055
PstI CTGCAG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 692
RsaI GTAC 1 cut(s) 575
RsaNI GTAC 1 cut(s) 574
RseI CAYNNNNRTG 1 cut(s) 866
SaqAI TTAA 2 cut(s) 159, 711
SatI GCNGC 1 cut(s) 164
Sau3AI GATC 1 cut(s) 692
Sau96I GGNCC 3 cut(s) 290, 623, 887
ScrFI CCNGG 2 cut(s) 431, 896
SduI GDGCHC 1 cut(s) 804
SfaNI GCATC 4 cut(s) 168, 454, 867, 933
SfcI CTRYAG 1 cut(s) 775
SinI GGWCC 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 949
SmoI CTYRAG 1 cut(s) 949
Sse9I AATT 5 cut(s) 172, 309, 761, 929, 1010
SsiI CCGC 3 cut(s) 164, 885, 940
SspMI CTAG 3 cut(s) 228, 426, 845
StyD4I CCNGG 2 cut(s) 429, 894
StyI CCWWGG 1 cut(s) 1026
TaaI ACNGT 2 cut(s) 204, 1044
TaiI ACGT 2 cut(s) 93, 110
TaqI TCGA 1 cut(s) 170
TasI AATT 5 cut(s) 172, 309, 761, 929, 1010
TatI WGTACW 1 cut(s) 573
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 2 cut(s) 533, 1065
Tru1I TTAA 2 cut(s) 159, 711
Tru9I TTAA 2 cut(s) 159, 711
TscAI CASTG 5 cut(s) 54, 372, 686, 704, 1027
TseFI GTSAC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 7 cut(s) 203, 350, 351, 398, 508, 922, 950
TspRI CASTG 5 cut(s) 54, 372, 686, 704, 1027
Van91I CCANNNNNTGG 1 cut(s) 513
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 4 cut(s) 172, 761, 929, 1010
XceI RCATGY 1 cut(s) 789
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XspI CTAG 3 cut(s) 228, 426, 845
Zsp2I ATGCAT 2 cut(s) 493, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.