pycom08g06110

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005609
Physical Location & Seq
Reverse (-)
4677633 .. 4679475
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06110.1

Sequence Viewer

Length: 1245 bp
ATGGTTAATTTAAAGTCCAGTTTACTATCTGTGATGCGAGTTGTGATTTTGGACTCTGCAATTTGTGCTGGTTTGAGAGAAAACTTGCAAATGAGGGTTGTGCCAGTGGAGGAAGGGAAGGATGCCGGTGTTAGTAGCAGCCGCCCAAGCCAGACGAAGGCCTTGCCGATAAGGCTACTTCAGTTATTCGCGCTGTTTCTGGTTTTCTGTATTACTTTCTCAGTCATTAGCATATACACAATTCGGCATTTTGGAATCAATGGTGTGATGACAACAGTCGCGGCCTCTTTCCAGCCGTGCCCTGAGGAACCAAAAGGTTTGGATCGATGGATTAAGCCGCCATCAAGTGTTAAACACACCATGAATGATGAGGAATTGTTGTGGAGGGCTTCTTTTACGCCTAGGATAAAGAAATATCCTTTTGAAAGGGTACCAAAGATTGCATTTATGTTCTTAACTAAGGGGCCGTTGCCACTGGCACCCCTTTGGGAGAGGTTACTGAAGGGACACGAAGGGCTTTATTCAGTCTACGTTCATTCACTACCATCGTTTCAACCCCATTTTAATCCTTCGTCGGTTTTTTATGGGAGACATATTCCAAGCCAGGTTGCTGAGTGGGGAAGAATGAGTATGTGTGATGCCGAGAGAAGACTCCTTGCTAATGCATTGCTTGACATATCCAATGAACGGTTCATTCTTCTTTCTGAATCATGCATTCCGCTCTATAATTTCAGTGTCATTTACCACTACTTGATGAAGTCCAAGTACAGCTATGTGGGTGCATTTGATGACCCCGGGCCATATGGAAGAGGACGCTACAATGACAACATGGCACCTGAAGTGAAAATATCCAAGTGGCGTAAGGGCTCTCAGTGGTTTGAAGTTAACCGAAAGCTCGCCATCACCATTGTCGAGGATACAAAATTTTACCCCAAATTTAAAGAGTTCTGCAGACCCGCATGTTACGTTGATGAGCACTACTTCCCCACCATGCTAACCATTGAAGCTGGGAATTCTTTGGCAAATAGAAGCATCACTTGGGTAGATTGGTCAAGGGGTGGTCCTCACCCGGCTACCTTTGGAAGAGCGGATATCACCGAGGAATTTTTGAAGCGAATTTTTGAGACTCAACGTTGCACTTACAATGACCGGAACTCTACAATCTGCTTTCTTTTCGGAAGGAAGTTTGCTCCAAGTGCCATGGAACCTCTTTTGCATTTATCACCAAAGTTTTTGGGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

415

Amino Acids

47.49

Weight (kDa)

9.27

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 147 - 373 2.2e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 430
AccB1I GGYRCC 3 cut(s) 430, 478, 832
AccBSI CCGCTC 2 cut(s) 721, 1088
AccI GTMKAC 1 cut(s) 528
AccII CGCG 2 cut(s) 191, 281
AciI CCGC 6 cut(s) 142, 281, 338, 719, 957, 1088
AclI AACGTT 1 cut(s) 1132
AclWI GGATC 1 cut(s) 330
AcsI RAATTY 5 cut(s) 923, 935, 1012, 1103, 1116
AcuI CTGAAG 3 cut(s) 164, 521, 858
AfaI GTAC 2 cut(s) 432, 767
AfiI CCNNNNNNNGG 2 cut(s) 157, 687
AgsI TTSAA 5 cut(s) 425, 554, 881, 1004, 1111
AjnI CCWGG 1 cut(s) 603
AjuI GAANNNNNNNTTGG 4 cut(s) 674, 706, 1021, 1053
AluBI AGCT 3 cut(s) 771, 895, 1007
AluI AGCT 3 cut(s) 771, 895, 1007
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 2 cut(s) 583, 1118
AlwI GGATC 1 cut(s) 330
Ama87I CYCGRG 1 cut(s) 794
AoxI GGCC 4 cut(s) 159, 282, 464, 797
ApeKI GCWGC 1 cut(s) 138
ApoI RAATTY 5 cut(s) 923, 935, 1012, 1103, 1116
Asp718I GGTACC 1 cut(s) 430
AspA2I CCTAGG 1 cut(s) 401
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 3 cut(s) 464, 797, 1061
AsuC2I CCSGG 3 cut(s) 795, 796, 1070
AsuHPI GGTGA 4 cut(s) 895, 1058, 1087, 1215
AvaI CYCGRG 1 cut(s) 794
AvaII GGWCC 1 cut(s) 1061
AvrII CCTAGG 1 cut(s) 401
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 302
BanI GGYRCC 3 cut(s) 430, 478, 832
BanII GRGCYC 1 cut(s) 869
BarI GAAGNNNNNNTAC 2 cut(s) 749, 781
BbsI GAAGAC 1 cut(s) 655
Bbv12I GWGCWC 1 cut(s) 978
BbvI GCAGC 1 cut(s) 150
BccI CCATC 4 cut(s) 321, 349, 553, 908
BceAI ACGGC 2 cut(s) 280, 451
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 1 cut(s) 605
BciVI GTATCC 1 cut(s) 910
BcnI CCSGG 3 cut(s) 795, 796, 1070
BcoDI GTCTC 2 cut(s) 583, 1118
BfaI CTAG 1 cut(s) 402
BfmI CTRYAG 1 cut(s) 949
BfuI GTATCC 1 cut(s) 910
BglI GCCNNNNNGGC 1 cut(s) 172
BisI GCNGC 4 cut(s) 139, 142, 282, 338
BlnI CCTAGG 1 cut(s) 401
BlsI GCNGC 4 cut(s) 140, 143, 283, 339
Bme1390I CCNGG 4 cut(s) 605, 795, 796, 1070
Bme18I GGWCC 1 cut(s) 1061
BmeT110I CYCGRG 1 cut(s) 794
BmgT120I GGNCC 3 cut(s) 464, 797, 1061
BmiI GGNNCC 6 cut(s) 309, 432, 465, 480, 834, 1206
BmrFI CCNGG 4 cut(s) 605, 795, 796, 1070
BmsI GCATC 4 cut(s) 24, 112, 628, 1041
BoxI GACNNNNGTC 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 655
BpuMI CCSGG 3 cut(s) 795, 796, 1070
Bsa29I ATCGAT 1 cut(s) 325
BsaJI CCNNGG 5 cut(s) 401, 793, 794, 1098, 1200
BsaWI WCCGGW 1 cut(s) 1149
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 687
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 18, 104, 480
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 665
BseBI CCWGG 1 cut(s) 605
BseCI ATCGAT 1 cut(s) 325
BseDI CCNNGG 5 cut(s) 401, 793, 794, 1098, 1200
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 2 cut(s) 157, 687
BseMI GCAATG 1 cut(s) 665
BseMII CTCAG 4 cut(s) 234, 294, 603, 884
BseNI ACTGG 3 cut(s) 18, 104, 480
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 1 cut(s) 150
BseYI CCCAGC 1 cut(s) 1007
Bsh1236I CGCG 2 cut(s) 191, 281
BshFI GGCC 4 cut(s) 161, 284, 466, 799
BshNI GGYRCC 3 cut(s) 430, 478, 832
BshVI ATCGAT 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 978
BsiHKCI CYCGRG 1 cut(s) 794
BsiSI CCGG 4 cut(s) 126, 795, 1070, 1150
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 2 cut(s) 157, 687
BsmAI GTCTC 2 cut(s) 583, 1118
BsmFI GGGAC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 714
BsnI GGCC 4 cut(s) 161, 284, 466, 799
BsoBI CYCGRG 1 cut(s) 794
Bsp1286I GDGCHC 3 cut(s) 302, 869, 978
Bsp143I GATC 1 cut(s) 322
Bsp19I CCATGG 1 cut(s) 1200
BspACI CCGC 6 cut(s) 142, 281, 338, 719, 957, 1088
BspANI GGCC 4 cut(s) 161, 284, 466, 799
BspCNI CTCAG 4 cut(s) 233, 295, 604, 883
BspDI ATCGAT 1 cut(s) 325
BspFNI CGCG 2 cut(s) 191, 281
BspLI GGNNCC 6 cut(s) 309, 432, 465, 480, 834, 1206
BspMAI CTGCAG 1 cut(s) 953
BspPI GGATC 1 cut(s) 330
BspQI GCTCTTC 1 cut(s) 1078
BspT107I GGYRCC 3 cut(s) 430, 478, 832
BsrBI CCGCTC 2 cut(s) 721, 1088
BsrDI GCAATG 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 3 cut(s) 18, 104, 480
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 5 cut(s) 401, 793, 794, 1098, 1200
BssMI GATC 1 cut(s) 322
BssT1I CCWWGG 2 cut(s) 401, 1200
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 2 cut(s) 277, 690
Bst6I CTCTTC 2 cut(s) 802, 1078
BstAPI GCANNNNNTGC 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 897
BstDEI CTNAG 5 cut(s) 220, 303, 459, 612, 870
BstDSI CCRYGG 1 cut(s) 1200
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 2 cut(s) 191, 281
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 1 cut(s) 325
BstMAI GTCTC 2 cut(s) 583, 1118
BstMBI GATC 1 cut(s) 322
BstMWI GCNNNNNNNGC 4 cut(s) 65, 147, 172, 1196
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 963
BstPAI GACNNNNGTC 1 cut(s) 275
BstSCI CCNGG 4 cut(s) 603, 793, 794, 1068
BstSFI CTRYAG 1 cut(s) 949
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 2 cut(s) 191, 281
BstV1I GCAGC 1 cut(s) 150
BstV2I GAAGAC 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 325
Bsu36I CCTNAGG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 910
BsuRI GGCC 4 cut(s) 161, 284, 466, 799
BsuTUI ATCGAT 1 cut(s) 325
BtgI CCRYGG 1 cut(s) 1200
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 4 cut(s) 111, 473, 739, 878
Cac8I GCNNGC 1 cut(s) 897
CfoI GCGC 1 cut(s) 193
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 464, 797, 1061
Cfr9I CCCGGG 1 cut(s) 794
ClaI ATCGAT 1 cut(s) 325
CseI GACGC 1 cut(s) 822
Csp6I GTAC 2 cut(s) 431, 766
CviAII CATG 6 cut(s) 361, 711, 829, 960, 991, 1201
CviQI GTAC 2 cut(s) 431, 766
DdeI CTNAG 5 cut(s) 220, 303, 459, 612, 870
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
DraI TTTAAA 2 cut(s) 12, 940
Eam1104I CTCTTC 2 cut(s) 802, 1078
EarI CTCTTC 2 cut(s) 802, 1078
Eco130I CCWWGG 2 cut(s) 401, 1200
Eco147I AGGCCT 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 869
Eco32I GATATC 1 cut(s) 1093
Eco47I GGWCC 1 cut(s) 1061
Eco57I CTGAAG 3 cut(s) 164, 521, 858
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 794
EcoRI GAATTC 1 cut(s) 1012
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 1093
EcoT14I CCWWGG 2 cut(s) 401, 1200
EcoT22I ATGCAT 2 cut(s) 667, 716
EcoT38I GRGCYC 1 cut(s) 869
ErhI CCWWGG 2 cut(s) 401, 1200
FaeI CATG 6 cut(s) 364, 714, 832, 963, 994, 1204
FalI AAGNNNNNCTT 2 cut(s) 1021, 1053
FaqI GGGAC 1 cut(s) 519
FatI CATG 6 cut(s) 360, 710, 828, 959, 990, 1200
FauI CCCGC 1 cut(s) 964
FauNDI CATATG 1 cut(s) 802
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 4 cut(s) 139, 142, 282, 338
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 869
Fsp4HI GCNGC 4 cut(s) 139, 142, 282, 338
FspBI CTAG 1 cut(s) 402
GlaI GCGC 1 cut(s) 192
GluI GCNGC 4 cut(s) 139, 142, 282, 338
GsaI CCCAGC 1 cut(s) 1011
HaeIII GGCC 4 cut(s) 161, 284, 466, 799
HapII CCGG 4 cut(s) 126, 795, 1070, 1150
HgaI GACGC 1 cut(s) 822
HhaI GCGC 1 cut(s) 193
Hin1II CATG 6 cut(s) 364, 714, 832, 963, 994, 1204
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 886
HindII GTYRAC 1 cut(s) 886
HinfI GANTC 5 cut(s) 53, 255, 651, 707, 1126
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 4 cut(s) 126, 795, 1070, 1150
HphI GGTGA 4 cut(s) 895, 1058, 1087, 1215
Hpy166II GTNNAC 3 cut(s) 23, 529, 886
Hpy188I TCNGA 2 cut(s) 706, 1178
Hpy8I GTNNAC 3 cut(s) 23, 529, 886
Hpy99I CGWCG 1 cut(s) 577
HpyAV CCTTC 7 cut(s) 107, 112, 151, 496, 506, 579, 1173
HpyCH4III ACNGT 2 cut(s) 277, 690
HpyCH4IV ACGT 3 cut(s) 531, 966, 1132
HpyCH4V TGCA 9 cut(s) 59, 88, 443, 665, 714, 782, 951, 1137, 1216
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 147, 172, 1196
HpyF3I CTNAG 5 cut(s) 220, 303, 459, 612, 870
HpySE526I ACGT 3 cut(s) 531, 966, 1132
Hsp92II CATG 6 cut(s) 364, 714, 832, 963, 994, 1204
HspAI GCGC 1 cut(s) 191
KpnI GGTACC 1 cut(s) 434
KspAI GTTAAC 1 cut(s) 886
Kzo9I GATC 1 cut(s) 322
LguI GCTCTTC 1 cut(s) 1078
LmnI GCTCC 1 cut(s) 1195
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 4 cut(s) 24, 112, 628, 1041
MaeI CTAG 1 cut(s) 402
MaeII ACGT 3 cut(s) 531, 966, 1132
MaeIII GTNAC 2 cut(s) 495, 962
MalI GATC 1 cut(s) 324
MbiI CCGCTC 2 cut(s) 721, 1088
MboI GATC 1 cut(s) 322
MboII GAAGA 5 cut(s) 633, 660, 689, 819, 1095
MhlI GDGCHC 3 cut(s) 302, 869, 978
MlyI GAGTC 3 cut(s) 47, 645, 1120
Mph1103I ATGCAT 2 cut(s) 667, 716
MseI TTAA 8 cut(s) 6, 11, 333, 351, 455, 564, 885, 939
MspI CCGG 4 cut(s) 126, 795, 1070, 1150
MspR9I CCNGG 4 cut(s) 605, 795, 796, 1070
Mva1269I GAATGC 1 cut(s) 714
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 2 cut(s) 191, 281
MwoI GCNNNNNNNGC 4 cut(s) 65, 147, 172, 1196
NciI CCSGG 3 cut(s) 795, 796, 1070
NcoI CCATGG 1 cut(s) 1200
NdeI CATATG 1 cut(s) 802
NdeII GATC 1 cut(s) 322
NlaIII CATG 6 cut(s) 364, 714, 832, 963, 994, 1204
NlaIV GGNNCC 6 cut(s) 309, 432, 465, 480, 834, 1206
NmeAIII GCCGAG 1 cut(s) 667
NsiI ATGCAT 2 cut(s) 667, 716
NspI RCATGY 1 cut(s) 963
PceI AGGCCT 1 cut(s) 161
PciSI GCTCTTC 1 cut(s) 1078
PctI GAATGC 1 cut(s) 714
PfeI GAWTC 2 cut(s) 255, 707
PkrI GCNGC 4 cut(s) 140, 143, 283, 339
PleI GAGTC 3 cut(s) 47, 645, 1120
PpsI GAGTC 3 cut(s) 47, 645, 1120
PshAI GACNNNNGTC 1 cut(s) 275
Psp1406I AACGTT 1 cut(s) 1132
Psp6I CCWGG 1 cut(s) 603
PspFI CCCAGC 1 cut(s) 1007
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 6 cut(s) 309, 432, 465, 480, 834, 1206
PspPI GGNCC 3 cut(s) 464, 797, 1061
PstI CTGCAG 1 cut(s) 953
RsaI GTAC 2 cut(s) 432, 767
RsaNI GTAC 2 cut(s) 431, 766
SapI GCTCTTC 1 cut(s) 1078
SaqAI TTAA 8 cut(s) 6, 11, 333, 351, 455, 564, 885, 939
SatI GCNGC 4 cut(s) 139, 142, 282, 338
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 3 cut(s) 464, 797, 1061
SchI GAGTC 3 cut(s) 47, 645, 1120
ScrFI CCNGG 4 cut(s) 605, 795, 796, 1070
SduI GDGCHC 3 cut(s) 302, 869, 978
SfaNI GCATC 4 cut(s) 24, 112, 628, 1041
SfcI CTRYAG 1 cut(s) 949
SinI GGWCC 1 cut(s) 1061
SmaI CCCGGG 1 cut(s) 796
SseBI AGGCCT 1 cut(s) 161
SsiI CCGC 6 cut(s) 142, 281, 338, 719, 957, 1088
SspMI CTAG 1 cut(s) 402
StuI AGGCCT 1 cut(s) 161
StyD4I CCNGG 4 cut(s) 603, 793, 794, 1068
StyI CCWWGG 2 cut(s) 401, 1200
TaaI ACNGT 2 cut(s) 277, 690
TaiI ACGT 3 cut(s) 534, 969, 1135
TaqI TCGA 2 cut(s) 325, 912
TatI WGTACW 1 cut(s) 765
TauI GCSGC 3 cut(s) 144, 284, 340
TfiI GAWTC 2 cut(s) 255, 707
Tru1I TTAA 8 cut(s) 6, 11, 333, 351, 455, 564, 885, 939
Tru9I TTAA 8 cut(s) 6, 11, 333, 351, 455, 564, 885, 939
TscAI CASTG 4 cut(s) 111, 480, 739, 878
TseI GCWGC 1 cut(s) 138
TspDTI ATGAA 5 cut(s) 377, 524, 682, 699, 770
TspMI CCCGGG 1 cut(s) 794
TspRI CASTG 4 cut(s) 111, 480, 739, 878
VpaK11BI GGWCC 1 cut(s) 1061
XapI RAATTY 5 cut(s) 923, 935, 1012, 1103, 1116
XceI RCATGY 1 cut(s) 963
XmaI CCCGGG 1 cut(s) 794
XmaJI CCTAGG 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 1 cut(s) 402
Zsp2I ATGCAT 2 cut(s) 667, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.