Rorug06G0380400

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005609
Physical Location & Seq
Forward (+)
52622904 .. 52623269
366 bp
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UTR
Exon/CDS
Intron
Rorug06G0380400.1

Sequence Viewer

Length: 366 bp
ATGCGTTGTTGTTTGTTCATTAAGGAGGGAAATGAGGAGGAGGAGCTGGAGCTGAATCTGGGGTTGTCTTTGGGAGGAAGATTCGGAGTGGACAAGAGTGAGAAAAGCAAGCTAGTCCGATCTTCCTCGATTGCGTTGACTATGCCATTTTGTAGGGAAGAGAACGATGACTCGGCGGCGAAGGCGGCGTCGTACTCGGGGCTGATGAGGGCGTCATCACTTCCGACGGAGACGGAGGAGGAGTGGAGGAAGAGGAAGGAGTTGCAGACGCTGCGGCGGTTGGCGGCGAAGCGGAGGAGGTCAGACAAGCAGAGGAACAACAGCAGCAGCAAGGAGGAAGAGCTGGCATTAACTAGTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

121

Amino Acids

13.68

Weight (kDa)

8.49

Isoelectric Point (pI)

85.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAR PF07897 11 - 45 1.4e-16 Ethylene-responsive binding factor-associated repression
NLS_NINJA_AFP PF16136 68 - 104 6.6e-21 Putative nuclear localisation signal in Ninja/AFP proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 176, 185, 274, 277, 284, 292
AcyI GRCGYC 2 cut(s) 188, 212
AfaI GTAC 1 cut(s) 194
AhlI ACTAGT 1 cut(s) 353
AluBI AGCT 4 cut(s) 46, 52, 112, 343
AluI AGCT 4 cut(s) 46, 52, 112, 343
Alw26I GTCTC 1 cut(s) 224
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 196
ApeKI GCWGC 3 cut(s) 271, 324, 327
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AvaI CYCGRG 1 cut(s) 196
BbvI GCAGC 3 cut(s) 258, 336, 339
BcoDI GTCTC 1 cut(s) 224
BcuI ACTAGT 1 cut(s) 353
BfaI CTAG 2 cut(s) 113, 354
BisI GCNGC 7 cut(s) 177, 186, 272, 275, 285, 325, 328
BlsI GCNGC 7 cut(s) 178, 187, 273, 276, 286, 326, 329
BmeT110I CYCGRG 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 68
BsaHI GRCGYC 2 cut(s) 188, 212
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
BseRI GAGGAG 6 cut(s) 50, 53, 56, 251, 254, 310
BseXI GCAGC 3 cut(s) 258, 336, 339
BsiHKCI CYCGRG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 224
BsmBI CGTCTC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 196
Bsp143I GATC 1 cut(s) 119
BspACI CCGC 6 cut(s) 176, 185, 274, 277, 284, 292
BspHI TCATGA 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 333
BssMI GATC 1 cut(s) 119
BssNI GRCGYC 2 cut(s) 188, 212
Bst6I CTCTTC 3 cut(s) 153, 245, 333
BstACI GRCGYC 2 cut(s) 188, 212
BstAPI GCANNNNNTGC 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 110, 345
BstKTI GATC 1 cut(s) 122
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 1 cut(s) 119
BstMWI GCNNNNNNNGC 3 cut(s) 182, 185, 271
BstV1I GCAGC 3 cut(s) 258, 336, 339
Cac8I GCNNGC 2 cut(s) 110, 345
CaiI CAGNNNCTG 1 cut(s) 271
CciI TCATGA 1 cut(s) 362
CseI GACGC 3 cut(s) 177, 201, 277
Csp6I GTAC 1 cut(s) 193
CviAII CATG 1 cut(s) 363
CviJI RGCY 5 cut(s) 46, 52, 112, 202, 343
CviKI_1 RGCY 5 cut(s) 46, 52, 112, 202, 343
CviQI GTAC 1 cut(s) 193
DpnI GATC 1 cut(s) 121
DpnII GATC 1 cut(s) 119
Eam1104I CTCTTC 3 cut(s) 153, 245, 333
EarI CTCTTC 3 cut(s) 153, 245, 333
Eco88I CYCGRG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 1 cut(s) 366
FaiI YATR 2 cut(s) 143, 364
FatI CATG 1 cut(s) 362
Fnu4HI GCNGC 7 cut(s) 177, 186, 272, 275, 285, 325, 328
Fsp4HI GCNGC 7 cut(s) 177, 186, 272, 275, 285, 325, 328
FspBI CTAG 2 cut(s) 113, 354
GluI GCNGC 7 cut(s) 177, 186, 272, 275, 285, 325, 328
GsuI CTGGAG 1 cut(s) 68
HgaI GACGC 3 cut(s) 177, 201, 277
Hin1I GRCGYC 2 cut(s) 188, 212
Hin1II CATG 1 cut(s) 366
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 3 cut(s) 55, 81, 170
Hpy166II GTNNAC 2 cut(s) 91, 138
Hpy188I TCNGA 4 cut(s) 86, 119, 225, 304
Hpy188III TCNNGA 1 cut(s) 363
Hpy8I GTNNAC 2 cut(s) 91, 138
Hpy99I CGWCG 2 cut(s) 193, 229
HpyAV CCTTC 2 cut(s) 175, 250
HpyCH4V TGCA 1 cut(s) 265
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 185, 271
Hsp92I GRCGYC 2 cut(s) 188, 212
Hsp92II CATG 1 cut(s) 366
Kzo9I GATC 1 cut(s) 119
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 2 cut(s) 43, 49
LpnPI CCDG 3 cut(s) 32, 44, 329
Lsp1109I GCAGC 3 cut(s) 258, 336, 339
MaeI CTAG 2 cut(s) 113, 354
MalI GATC 1 cut(s) 121
MboI GATC 1 cut(s) 119
MboII GAAGA 6 cut(s) 90, 114, 170, 262, 350, 351
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 248
MseI TTAA 2 cut(s) 21, 350
MwoI GCNNNNNNNGC 3 cut(s) 182, 185, 271
NdeII GATC 1 cut(s) 119
NlaIII CATG 1 cut(s) 366
NmeAIII GCCGAG 1 cut(s) 152
PagI TCATGA 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 55, 81
PkrI GCNGC 7 cut(s) 178, 187, 273, 276, 286, 326, 329
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
PstNI CAGNNNCTG 1 cut(s) 271
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 21, 350
SatI GCNGC 7 cut(s) 177, 186, 272, 275, 285, 325, 328
Sau3AI GATC 1 cut(s) 119
SchI GAGTC 1 cut(s) 164
SetI ASST 5 cut(s) 48, 54, 114, 302, 345
SpeI ACTAGT 1 cut(s) 353
SsiI CCGC 6 cut(s) 176, 185, 274, 277, 284, 292
SspMI CTAG 2 cut(s) 113, 354
TaqI TCGA 1 cut(s) 128
TauI GCSGC 4 cut(s) 179, 188, 277, 287
TfiI GAWTC 2 cut(s) 55, 81
Tru1I TTAA 2 cut(s) 21, 350
Tru9I TTAA 2 cut(s) 21, 350
TseI GCWGC 3 cut(s) 271, 324, 327
TspDTI ATGAA 2 cut(s) 7, 351
TspGWI ACGGA 2 cut(s) 242, 248
XspI CTAG 2 cut(s) 113, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.