Rroxscaffold_7G00158520

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005609
Physical Location & Seq
Reverse (-)
1932684 .. 1936072
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158520.1

Sequence Viewer

Length: 1254 bp
ATGCAATCAAGGGTTGTGCCATTAGAGGAAGGCAAGGACCCGGGAGTTAGTAATAGCAGGACGAACCAAACCAAGGCGTTACCGATTCGGATGCTTCAGTTATTTGTGCTGTTTCTGGCTTTCTGTATCACTTTCTCAGTGATTAGTATATACACCATTCGGCATTTTGGGATTAGTAACGTGATGACTTCAGTCACGTCCTCTGTCCAGCCATGTTTTGAGGAACCAACTGGTTTGGATAGATGGATTAAGCCGCCATCGAATTTGATGCACACTATGAATGACGAAGAACTGTTGTGGAGGGCTTCTTTGTCGTCTAAGAGAAAGAAGTATCCTTTTGAGAGAGTTCCGAAGATTGCGCTTATGTTCTTGACTAAGGGGCCTTTGCCTCTGGCACCAATTTGGGAGAGGTTTCTGAAGGGGCATGAAGGATTTTATTCAGTCTATGTTCATTCACTGCCATCATTTCAACCCCATTTCCCACCTTCCTCGGTTTTTCATGGGAGACATATTCCTAGCCAGGTTGCTGAATGGGGAAGGATGAGTATGTGTGATGCTGAGAGGAGACTTCTTGCTAATGCATTGCTTGACATATCCAACGAATGGTTCATTCTTGTTTCTGAATCATGCATTCCTCTCTTTAACTTCAGCGTCATTTACCAGTACATGAGGAAGTCCAAGTATAGCTACATGGGTGCGTTTGATGACCACGGCCCGTATGGCAGAGGACGCTACAATGATAACATGGCTCCTGAAGTGAAGATTACCCAGTGGCGTAAGGGATCCCAGTGGTTTGAAGTTAACCGGAAGCTTGCCATCGCCATCATTGAAGATACAACATTTTACCCAAAATTCAAAGAGTTCTGTAGACCATCATGTTATGTTGATGAGCACTACTTCCCCACCATGTTAACCATTCAAGCAGGGAATATGCTAGCAAACAGAAGCGTCACTTGGGTGGATTGGTCAAGGGGCGGACCTCACCCGGCCACCTTTGGCAGAGGAGATATCAATGAGGAATTCATGAAGCGGATGCTCTCAAGTCATCGTTGCACTTACAACAACCGAAACTCTACGATGTGCTTTCTATTTGCAAGAAAATTTGCTCCCAGTGCCTTGGAACCTCTCTTACATCTAGCACCAAAGTTTTTTGGGCTTCTGACATATATATCCACAGCCAACCACCATCGATCTCCAGCGCAGGATAAACAAGCTCACTTTGAAAAATTTACCCTCTCCTGTGATATTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

417

Amino Acids

48.27

Weight (kDa)

9.23

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 119 - 345 5.2e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccB7I CCANNNNNTGG 1 cut(s) 603
AccI GTMKAC 1 cut(s) 868
AciI CCGC 3 cut(s) 254, 975, 1030
AclWI GGATC 2 cut(s) 777, 790
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 5 cut(s) 262, 851, 1019, 1100, 1226
AcuI CTGAAG 5 cut(s) 80, 174, 437, 631, 774
AfaI GTAC 1 cut(s) 665
AfiI CCNNNNNNNGG 2 cut(s) 73, 603
AgsI TTSAA 6 cut(s) 470, 797, 830, 856, 920, 1223
AjiI CACGTC 1 cut(s) 198
AjnI CCWGG 1 cut(s) 519
AjuI GAANNNNNNNTTGG 4 cut(s) 590, 622, 937, 969
AleI CACNNNNGTG 1 cut(s) 956
AluBI AGCT 3 cut(s) 687, 811, 1214
AluI AGCT 3 cut(s) 687, 811, 1214
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 2 cut(s) 499, 559
AlwI GGATC 2 cut(s) 777, 790
Ama87I CYCGRG 1 cut(s) 40
AoxI GGCC 3 cut(s) 380, 712, 987
ApoI RAATTY 5 cut(s) 262, 851, 1019, 1100, 1226
AspLEI GCGC 2 cut(s) 361, 1201
AspS9I GGNCC 4 cut(s) 37, 380, 713, 977
AsuC2I CCSGG 3 cut(s) 41, 42, 986
AsuHPI GGTGA 1 cut(s) 974
AsuNHI GCTAGC 1 cut(s) 934
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 37, 977
BamHI GGATCC 1 cut(s) 782
BanI GGYRCC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 894
BccI CCATC 7 cut(s) 237, 265, 469, 824, 830, 880, 1194
BceAI ACGGC 1 cut(s) 727
BcgI CGANNNNNNTGC 4 cut(s) 73, 107, 250, 284
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 1 cut(s) 342
BcnI CCSGG 3 cut(s) 41, 42, 986
BcoDI GTCTC 2 cut(s) 499, 559
BfaI CTAG 4 cut(s) 516, 935, 1136, 1252
BfmI CTRYAG 1 cut(s) 865
BfuI GTATCC 1 cut(s) 342
BglI GCCNNNNNGGC 1 cut(s) 720
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
Bme1390I CCNGG 4 cut(s) 41, 42, 521, 986
Bme18I GGWCC 2 cut(s) 37, 977
BmeT110I CYCGRG 1 cut(s) 40
BmgBI CACGTC 1 cut(s) 198
BmgT120I GGNCC 4 cut(s) 37, 380, 713, 977
BmiI GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
BmrFI CCNGG 4 cut(s) 41, 42, 521, 986
BmrI ACTGGG 3 cut(s) 763, 781, 1104
BmsI GCATC 4 cut(s) 81, 258, 544, 1023
BmtI GCTAGC 1 cut(s) 938
BmuI ACTGGG 3 cut(s) 763, 781, 1104
BoxI GACNNNNGTC 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 1179
BpuEI CTTGAG 1 cut(s) 1024
BpuMI CCSGG 3 cut(s) 41, 42, 986
Bsa29I ATCGAT 1 cut(s) 1189
BsaJI CCNNGG 5 cut(s) 40, 72, 489, 709, 1116
BsaWI WCCGGW 1 cut(s) 804
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 603
Bse1I ACTGG 5 cut(s) 235, 661, 769, 787, 1110
Bse3DI GCAATG 1 cut(s) 581
BseBI CCWGG 1 cut(s) 521
BseCI ATCGAT 1 cut(s) 1189
BseDI CCNNGG 5 cut(s) 40, 72, 489, 709, 1116
BseGI GGATG 3 cut(s) 96, 546, 1038
BseLI CCNNNNNNNGG 2 cut(s) 73, 603
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 2 cut(s) 150, 549
BseNI ACTGG 5 cut(s) 235, 661, 769, 787, 1110
BseRI GAGGAG 2 cut(s) 577, 1017
BshFI GGCC 3 cut(s) 382, 714, 989
BshNI GGYRCC 1 cut(s) 394
BshVI ATCGAT 1 cut(s) 1189
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 3 cut(s) 41, 805, 986
BslI CCNNNNNNNGG 2 cut(s) 73, 603
BsmAI GTCTC 2 cut(s) 499, 559
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 3 cut(s) 382, 714, 989
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 894
Bsp143I GATC 2 cut(s) 782, 1190
BspACI CCGC 3 cut(s) 254, 975, 1030
BspANI GGCC 3 cut(s) 382, 714, 989
BspCNI CTCAG 2 cut(s) 149, 550
BspDI ATCGAT 1 cut(s) 1189
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
BspOI GCTAGC 1 cut(s) 938
BspPI GGATC 2 cut(s) 777, 790
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 581
BsrI ACTGG 5 cut(s) 235, 661, 769, 787, 1110
BssECI CCNNGG 5 cut(s) 40, 72, 489, 709, 1116
BssMI GATC 2 cut(s) 782, 1190
BssT1I CCWWGG 2 cut(s) 72, 1116
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 813, 936
BstDEI CTNAG 4 cut(s) 136, 318, 375, 558
BstDSI CCRYGG 1 cut(s) 709
BstF5I GGATG 3 cut(s) 96, 546, 1038
BstHHI GCGC 2 cut(s) 361, 1201
BstKTI GATC 2 cut(s) 785, 1193
BstMAI GTCTC 2 cut(s) 499, 559
BstMBI GATC 2 cut(s) 782, 1190
BstMWI GCNNNNNNNGC 3 cut(s) 720, 729, 1112
BstNI CCWGG 1 cut(s) 521
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 4 cut(s) 39, 40, 519, 984
BstSFI CTRYAG 1 cut(s) 865
BstX2I RGATCY 1 cut(s) 782
BstXI CCANNNNNNTGG 1 cut(s) 1117
BstYI RGATCY 1 cut(s) 782
Bsu15I ATCGAT 1 cut(s) 1189
BsuI GTATCC 1 cut(s) 342
BsuRI GGCC 3 cut(s) 382, 714, 989
BsuTUI ATCGAT 1 cut(s) 1189
BtgI CCRYGG 1 cut(s) 709
BtgZI GCGATG 1 cut(s) 802
BtrI CACGTC 1 cut(s) 198
BtsCI GGATG 3 cut(s) 96, 546, 1038
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 5 cut(s) 144, 455, 776, 794, 1117
Cac8I GCNNGC 2 cut(s) 813, 936
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 2 cut(s) 361, 1201
Cfr13I GGNCC 4 cut(s) 37, 380, 713, 977
Cfr9I CCCGGG 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 1189
CseI GACGC 3 cut(s) 640, 738, 937
Csp6I GTAC 1 cut(s) 664
CviQI GTAC 1 cut(s) 664
DdeI CTNAG 4 cut(s) 136, 318, 375, 558
DpnI GATC 2 cut(s) 784, 1192
DpnII GATC 2 cut(s) 782, 1190
EaeI YGGCCR 1 cut(s) 987
EciI GGCGGA 1 cut(s) 990
Eco130I CCWWGG 2 cut(s) 72, 1116
Eco32I GATATC 1 cut(s) 1009
Eco47I GGWCC 2 cut(s) 37, 977
Eco57I CTGAAG 5 cut(s) 80, 174, 437, 631, 774
Eco88I CYCGRG 1 cut(s) 40
EcoO109I RGGNCCY 2 cut(s) 37, 380
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 519
EcoRV GATATC 1 cut(s) 1009
EcoT14I CCWWGG 2 cut(s) 72, 1116
EcoT22I ATGCAT 2 cut(s) 583, 632
ErhI CCWWGG 2 cut(s) 72, 1116
FalI AAGNNNNNCTT 2 cut(s) 937, 969
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 3 cut(s) 103, 553, 1045
Fsp4HI GCNGC 1 cut(s) 254
FspBI CTAG 4 cut(s) 516, 935, 1136, 1252
GlaI GCGC 2 cut(s) 360, 1200
GluI GCNGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 1179
HaeIII GGCC 3 cut(s) 382, 714, 989
HapII CCGG 3 cut(s) 41, 805, 986
HgaI GACGC 3 cut(s) 640, 738, 937
HhaI GCGC 2 cut(s) 361, 1201
Hin6I GCGC 2 cut(s) 359, 1199
HinP1I GCGC 2 cut(s) 359, 1199
HincII GTYRAC 2 cut(s) 802, 912
HindII GTYRAC 2 cut(s) 802, 912
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 2 cut(s) 85, 623
HpaI GTTAAC 2 cut(s) 802, 912
HpaII CCGG 3 cut(s) 41, 805, 986
HphI GGTGA 1 cut(s) 974
Hpy166II GTNNAC 3 cut(s) 802, 869, 912
Hpy188I TCNGA 5 cut(s) 90, 351, 417, 622, 1161
Hpy188III TCNNGA 3 cut(s) 370, 752, 1024
Hpy8I GTNNAC 3 cut(s) 802, 869, 912
HpyAV CCTTC 5 cut(s) 23, 412, 422, 495, 531
HpyCH4III ACNGT 1 cut(s) 294
HpyCH4IV ACGT 2 cut(s) 180, 197
HpyCH4V TGCA 6 cut(s) 4, 271, 581, 630, 1053, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 720, 729, 1112
HpyF3I CTNAG 4 cut(s) 136, 318, 375, 558
HpySE526I ACGT 2 cut(s) 180, 197
HspAI GCGC 2 cut(s) 359, 1199
KspAI GTTAAC 2 cut(s) 802, 912
Kzo9I GATC 2 cut(s) 782, 1190
LmnI GCTCC 2 cut(s) 754, 1111
LweI GCATC 4 cut(s) 81, 258, 544, 1023
MaeI CTAG 4 cut(s) 516, 935, 1136, 1252
MaeII ACGT 2 cut(s) 180, 197
MaeIII GTNAC 4 cut(s) 78, 176, 193, 949
MalI GATC 2 cut(s) 784, 1192
MboI GATC 2 cut(s) 782, 1190
MboII GAAGA 4 cut(s) 299, 364, 772, 842
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 1 cut(s) 894
MluCI AATT 6 cut(s) 262, 399, 851, 1019, 1100, 1226
MmeI TCCRAC 1 cut(s) 621
Mph1103I ATGCAT 2 cut(s) 583, 632
MseI TTAA 4 cut(s) 249, 642, 801, 911
MslI CAYNNNNRTG 1 cut(s) 956
MspI CCGG 3 cut(s) 41, 805, 986
MspR9I CCNGG 4 cut(s) 41, 42, 521, 986
Mva1269I GAATGC 1 cut(s) 630
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 3 cut(s) 720, 729, 1112
NciI CCSGG 3 cut(s) 41, 42, 986
NdeII GATC 2 cut(s) 782, 1190
NheI GCTAGC 1 cut(s) 934
NlaIV GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
NmuCI GTSAC 2 cut(s) 193, 949
NsiI ATGCAT 2 cut(s) 583, 632
OliI CACNNNNGTG 1 cut(s) 956
PagI TCATGA 1 cut(s) 1023
PctI GAATGC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 85, 623
PflMI CCANNNNNTGG 1 cut(s) 603
PkrI GCNGC 1 cut(s) 255
PpuMI RGGWCCY 1 cut(s) 37
PshAI GACNNNNGTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
PspPI GGNCC 4 cut(s) 37, 380, 713, 977
PspPPI RGGWCCY 1 cut(s) 37
PsrI GAACNNNNNNTAC 2 cut(s) 1113, 1145
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 1 cut(s) 665
RsaNI GTAC 1 cut(s) 664
RseI CAYNNNNRTG 1 cut(s) 956
SaqAI TTAA 4 cut(s) 249, 642, 801, 911
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 2 cut(s) 782, 1190
Sau96I GGNCC 4 cut(s) 37, 380, 713, 977
ScrFI CCNGG 4 cut(s) 41, 42, 521, 986
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 4 cut(s) 81, 258, 544, 1023
SfcI CTRYAG 1 cut(s) 865
SinI GGWCC 2 cut(s) 37, 977
SmaI CCCGGG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 1 cut(s) 1039
SmoI CTYRAG 1 cut(s) 1039
Sse9I AATT 6 cut(s) 262, 399, 851, 1019, 1100, 1226
SsiI CCGC 3 cut(s) 254, 975, 1030
SspMI CTAG 4 cut(s) 516, 935, 1136, 1252
StyD4I CCNGG 4 cut(s) 39, 40, 519, 984
StyI CCWWGG 2 cut(s) 72, 1116
TaaI ACNGT 1 cut(s) 294
TaiI ACGT 2 cut(s) 183, 200
TaqI TCGA 2 cut(s) 260, 1189
TasI AATT 6 cut(s) 262, 399, 851, 1019, 1100, 1226
TatI WGTACW 1 cut(s) 663
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 2 cut(s) 85, 623
Tru1I TTAA 4 cut(s) 249, 642, 801, 911
Tru9I TTAA 4 cut(s) 249, 642, 801, 911
TscAI CASTG 5 cut(s) 144, 462, 776, 794, 1117
TseFI GTSAC 2 cut(s) 193, 949
Tsp45I GTSAC 2 cut(s) 193, 949
TspDTI ATGAA 7 cut(s) 293, 440, 441, 488, 598, 1012, 1040
TspMI CCCGGG 1 cut(s) 40
TspRI CASTG 5 cut(s) 144, 462, 776, 794, 1117
Van91I CCANNNNNTGG 1 cut(s) 603
VpaK11BI GGWCC 2 cut(s) 37, 977
XapI RAATTY 5 cut(s) 262, 851, 1019, 1100, 1226
XcmI CCANNNNNNNNNTGG 1 cut(s) 716
XmaI CCCGGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 868
XspI CTAG 4 cut(s) 516, 935, 1136, 1252
Zsp2I ATGCAT 2 cut(s) 583, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.