AT5G13460

IQ-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010030
Physical Location & Seq
Forward (+)
4315759 .. 4318414
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13460.1

Sequence Viewer

Length: 1332 bp
ATGGCTAAGAAGAAGGGCTTGTTCACTGTATTGAAAAGGATTTTTATTTCAGAAGTTAATTCAGAAAAGAAAGAGAAGAGAAGAAAATGGACATTTTGGAAGCTTAGGATTAAGAAAAGATTACCTTCCATTACAGCACCTCCAGAGCACAGGACAAGTCATGAATCGCATGAGGAACAGAAGGAGGAAATTGTGTCAGATGTGGGTGAGATCAGCCAAGTGTCTTGTAGTCGACAGTTAGATTCCATAGAAGAGTCAAAAGGTTCAACATCACCAGAAACTGCTGATCTGGTAGTCCAGTATCAAATGTTTCTTAATAGACAGGAAGAAGTTCTTGCTGCTACTCGCATTCAGACCGCCTTTCGGGGTCATCTTGCAAGGAAAGCTCTACGTGCCTTGAAGGGAATAGTGAAGCTCCAAGCATATATCAGAGGTCGTGCTGTGAGACGCCAAGCAATGACTACACTAAAATGCCTGCAATCTGTTGTGAACATTCAGTCACAAGTCTGTGGTAAGAGAACACAGATTCCCGGAGGTGTTCACAGAGATTATGAAGAGAGCAATATATTCAATGATAACATTCTCAAGGTGGACACAAACGGTCAAAAGAGATGGGACGATAGTCTTTTAACAAAGGAAGAAAAGGAAGCAGTGGTAATGAGCAAGAAAGAAGCTTCACTAAGAAGAGAAAGGATAAAGGAATATGCAGTCACCCACCGGAAATCTGCGGAGTCATACCAGAAACGAAGTAACACTAAATGGAAGTACTGGTTAGACGAATGGGTAGATACACAACTAACCAAGAGCAAGGAGCTCGAAGATCTCGACTTCTCTTCGAAAACAAAACCGAAAGACGAAACTTTGAACGAGAAGCAGCTTAAAACTCCAAGGAACTCATCACCAAGAAGATTAGTGAATAATCATAGAAGACAAGTTTCAATAGGTGAAGATGAACAAAGCCCTGCCGCGGTCACTATCACTACACCAACTTATATGGTTGCAACAGAGTCAGCAAAGGCAAAGTCAAGATCATTAAGCTCCCCAAGGATAAGACCGAGAAGTTTTGACACACAGTCAGAGAGTTACTCGCCATATAAGAACAAGCTATGCCTGACGACATCAATGATGAGTGAAGCACCAAGCAAAGTAAGGATTGCCAACAATGGCAGTAACACTAGACCAAGTGCATACCAGCAACGGTCTCCAGGGTTAAGGGGATTTAACATAGGCCCTTTGAAATCATGCAATAATAATAATACTCTATTGAACGATCTCAGCATTAATTCAGAAAGATCTCTACCTAGCTGGAACAAGCAGAGCAGCTTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

443

Amino Acids

50.82

Weight (kDa)

9.96

Isoelectric Point (pI)

69.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 307 - 375 1.2e-07 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 232
AccII CGCG 1 cut(s) 968
AciI CCGC 4 cut(s) 357, 728, 966, 968
AcyI GRCGYC 1 cut(s) 448
AfaI GTAC 1 cut(s) 767
AfiI CCNNNNNNNGG 2 cut(s) 363, 967
AgsI TTSAA 8 cut(s) 34, 267, 400, 571, 865, 939, 1237, 1267
AhdI GACNNNNNGTC 1 cut(s) 1072
AjnI CCWGG 1 cut(s) 1204
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
Alw21I GWGCWC 2 cut(s) 150, 816
Alw26I GTCTC 2 cut(s) 439, 1206
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 1 cut(s) 1228
ApeKI GCWGC 3 cut(s) 338, 874, 1320
ArsI GACNNNNNNTTYG 2 cut(s) 1007, 1039
AseI ATTAAT 1 cut(s) 1281
Asp700I GAANNNNTTC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 1229
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 5 cut(s) 218, 264, 703, 891, 956
AsuII TTCGAA 1 cut(s) 836
BanII GRGCYC 1 cut(s) 816
BbsI GAAGAC 1 cut(s) 934
Bbv12I GWGCWC 2 cut(s) 150, 816
BbvI GCAGC 2 cut(s) 325, 886
BccI CCATC 1 cut(s) 606
BcgI CGANNNNNNTGC 2 cut(s) 796, 830
BciT130I CCWGG 1 cut(s) 1206
BcnI CCSGG 1 cut(s) 531
BcoDI GTCTC 2 cut(s) 439, 1206
BfaI CTAG 2 cut(s) 1176, 1302
BglII AGATCT 2 cut(s) 820, 1292
BisI GCNGC 4 cut(s) 339, 875, 966, 1321
BlsI GCNGC 4 cut(s) 340, 876, 967, 1322
BmcAI AGTACT 1 cut(s) 767
Bme1390I CCNGG 2 cut(s) 531, 1206
BmeRI GACNNNNNGTC 1 cut(s) 1072
BmgT120I GGNCC 1 cut(s) 1229
BmrFI CCNGG 2 cut(s) 531, 1206
BoxI GACNNNNGTC 1 cut(s) 621
BpiI GAAGAC 1 cut(s) 934
BplI GAGNNNNNCTC 2 cut(s) 1070, 1102
BpmI CTGGAG 2 cut(s) 126, 1188
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 836
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 531
BsaAI YACGTR 1 cut(s) 392
BsaHI GRCGYC 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 1206
BsaJI CCNNGG 4 cut(s) 887, 966, 1043, 1205
BsaWI WCCGGW 1 cut(s) 717
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 967
Bse1I ACTGG 2 cut(s) 298, 773
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 1206
BseDI CCNNGG 4 cut(s) 887, 966, 1043, 1205
BseLI CCNNNNNNNGG 2 cut(s) 363, 967
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 1 cut(s) 1288
BseNI ACTGG 2 cut(s) 298, 773
BseXI GCAGC 2 cut(s) 325, 886
Bsh1236I CGCG 1 cut(s) 968
BshFI GGCC 1 cut(s) 1230
BsiHKAI GWGCWC 2 cut(s) 150, 816
BsiSI CCGG 2 cut(s) 531, 718
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 2 cut(s) 363, 967
BsmAI GTCTC 2 cut(s) 439, 1206
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 629
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 1230
Bso31I GGTCTC 1 cut(s) 1206
Bsp119I TTCGAA 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 150, 816
Bsp143I GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
BspACI CCGC 4 cut(s) 357, 728, 966, 968
BspANI GGCC 1 cut(s) 1230
BspCNI CTCAG 1 cut(s) 1287
BspFNI CGCG 1 cut(s) 968
BspHI TCATGA 1 cut(s) 160
BspT104I TTCGAA 1 cut(s) 836
BspTNI GGTCTC 1 cut(s) 1206
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 2 cut(s) 298, 773
BssECI CCNNGG 4 cut(s) 887, 966, 1043, 1205
BssMI GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
BssNI GRCGYC 1 cut(s) 448
BssT1I CCWWGG 2 cut(s) 887, 1043
Bst2UI CCWGG 1 cut(s) 1206
Bst4CI ACNGT 5 cut(s) 28, 237, 602, 1074, 1200
Bst6I CTCTTC 5 cut(s) 71, 246, 549, 679, 838
BstACI GRCGYC 1 cut(s) 448
BstBAI YACGTR 1 cut(s) 392
BstBI TTCGAA 1 cut(s) 836
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 4 cut(s) 6, 104, 680, 1274
BstDSI CCRYGG 1 cut(s) 966
BstFNI CGCG 1 cut(s) 968
BstKTI GATC 6 cut(s) 213, 289, 823, 1031, 1273, 1295
BstMAI GTCTC 2 cut(s) 439, 1206
BstMBI GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
BstMWI GCNNNNNNNGC 2 cut(s) 383, 392
BstNI CCWGG 1 cut(s) 1206
BstPAI GACNNNNGTC 1 cut(s) 621
BstSCI CCNGG 2 cut(s) 529, 1204
BstUI CGCG 1 cut(s) 968
BstV1I GCAGC 2 cut(s) 325, 886
BstV2I GAAGAC 1 cut(s) 934
BstX2I RGATCY 2 cut(s) 820, 1292
BstYI RGATCY 2 cut(s) 820, 1292
BsuRI GGCC 1 cut(s) 1230
BtgI CCRYGG 1 cut(s) 966
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 2 cut(s) 24, 657
Cac8I GCNNGC 1 cut(s) 476
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 1229
Cfr42I CCGCGG 1 cut(s) 969
CseI GACGC 1 cut(s) 456
Csp6I GTAC 1 cut(s) 766
CviAII CATG 3 cut(s) 161, 170, 1242
CviQI GTAC 1 cut(s) 766
DdeI CTNAG 4 cut(s) 6, 104, 680, 1274
DpnI GATC 6 cut(s) 212, 288, 822, 1030, 1272, 1294
DpnII GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
DriI GACNNNNNGTC 1 cut(s) 1072
Eam1104I CTCTTC 5 cut(s) 71, 246, 549, 679, 838
Eam1105I GACNNNNNGTC 1 cut(s) 1072
EarI CTCTTC 5 cut(s) 71, 246, 549, 679, 838
Ecl136II GAGCTC 1 cut(s) 814
Eco130I CCWWGG 2 cut(s) 887, 1043
Eco24I GRGCYC 1 cut(s) 816
Eco31I GGTCTC 1 cut(s) 1206
Eco53kI GAGCTC 1 cut(s) 814
EcoICRI GAGCTC 1 cut(s) 814
EcoO109I RGGNCCY 1 cut(s) 1229
EcoRII CCWGG 1 cut(s) 1204
EcoT14I CCWWGG 2 cut(s) 887, 1043
EcoT38I GRGCYC 1 cut(s) 816
ErhI CCWWGG 2 cut(s) 887, 1043
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 3 cut(s) 164, 173, 1245
FalI AAGNNNNNCTT 5 cut(s) 34, 109, 141, 318, 350
FaqI GGGAC 1 cut(s) 629
FatI CATG 3 cut(s) 160, 169, 1241
FblI GTMKAC 1 cut(s) 232
Fnu4HI GCNGC 4 cut(s) 339, 875, 966, 1321
FriOI GRGCYC 1 cut(s) 816
Fsp4HI GCNGC 4 cut(s) 339, 875, 966, 1321
FspBI CTAG 2 cut(s) 1176, 1302
GluI GCNGC 4 cut(s) 339, 875, 966, 1321
GsuI CTGGAG 2 cut(s) 126, 1188
HaeIII GGCC 1 cut(s) 1230
HapII CCGG 2 cut(s) 531, 718
HgaI GACGC 1 cut(s) 456
Hin1I GRCGYC 1 cut(s) 448
Hin1II CATG 3 cut(s) 164, 173, 1245
HincII GTYRAC 1 cut(s) 233
HindII GTYRAC 1 cut(s) 233
HindIII AAGCTT 2 cut(s) 101, 672
HinfI GANTC 6 cut(s) 164, 242, 254, 526, 731, 1007
HpaII CCGG 2 cut(s) 531, 718
HphI GGTGA 5 cut(s) 218, 264, 703, 891, 956
Hpy166II GTNNAC 5 cut(s) 24, 233, 490, 541, 592
Hpy188I TCNGA 7 cut(s) 52, 64, 199, 354, 431, 1078, 1288
Hpy188III TCNNGA 4 cut(s) 143, 161, 824, 1026
Hpy8I GTNNAC 5 cut(s) 24, 233, 490, 541, 592
HpyAV CCTTC 4 cut(s) 7, 135, 175, 394
HpyCH4III ACNGT 5 cut(s) 28, 237, 602, 1074, 1200
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 6 cut(s) 377, 478, 707, 1001, 1187, 1245
HpyF10VI GCNNNNNNNGC 2 cut(s) 383, 392
HpyF3I CTNAG 4 cut(s) 6, 104, 680, 1274
HpySE526I ACGT 1 cut(s) 391
Hsp92I GRCGYC 1 cut(s) 448
Hsp92II CATG 3 cut(s) 164, 173, 1245
KspI CCGCGG 1 cut(s) 969
Kzo9I GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
LmnI GCTCC 3 cut(s) 420, 811, 1043
Lsp1109I GCAGC 2 cut(s) 325, 886
MaeI CTAG 2 cut(s) 1176, 1302
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 6 cut(s) 498, 709, 749, 970, 1082, 1169
MalI GATC 6 cut(s) 212, 288, 822, 1030, 1272, 1294
MboI GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
MflI RGATCY 2 cut(s) 820, 1292
MhlI GDGCHC 2 cut(s) 150, 816
MluCI AATT 3 cut(s) 58, 189, 1282
MlyI GAGTC 3 cut(s) 263, 740, 1016
MnlI CCTC 5 cut(s) 150, 166, 178, 425, 527
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 9 cut(s) 57, 111, 315, 629, 879, 1034, 1211, 1221, 1281
MslI CAYNNNNRTG 1 cut(s) 469
MspA1I CMGCKG 1 cut(s) 968
MspI CCGG 2 cut(s) 531, 718
MspR9I CCNGG 2 cut(s) 531, 1206
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 1206
MvnI CGCG 1 cut(s) 968
MwoI GCNNNNNNNGC 2 cut(s) 383, 392
NciI CCSGG 1 cut(s) 531
NdeII GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
NlaIII CATG 3 cut(s) 164, 173, 1245
NmuCI GTSAC 3 cut(s) 498, 709, 970
NspV TTCGAA 1 cut(s) 836
PagI TCATGA 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 3 cut(s) 164, 242, 526
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 4 cut(s) 340, 876, 967, 1322
PleI GAGTC 3 cut(s) 262, 739, 1015
PpsI GAGTC 3 cut(s) 262, 739, 1015
Ppu21I YACGTR 1 cut(s) 392
PshAI GACNNNNGTC 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 1281
Psp124BI GAGCTC 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 1204
PspGI CCWGG 1 cut(s) 1204
PspPI GGNCC 1 cut(s) 1229
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 2 cut(s) 820, 1292
RsaI GTAC 1 cut(s) 767
RsaNI GTAC 1 cut(s) 766
RseI CAYNNNNRTG 1 cut(s) 469
SacI GAGCTC 1 cut(s) 816
SacII CCGCGG 1 cut(s) 969
SalI GTCGAC 1 cut(s) 231
SaqAI TTAA 9 cut(s) 57, 111, 315, 629, 879, 1034, 1211, 1221, 1281
SatI GCNGC 4 cut(s) 339, 875, 966, 1321
Sau3AI GATC 6 cut(s) 210, 286, 820, 1028, 1270, 1292
Sau96I GGNCC 1 cut(s) 1229
ScaI AGTACT 1 cut(s) 767
SchI GAGTC 3 cut(s) 263, 740, 1016
ScrFI CCNGG 2 cut(s) 531, 1206
SduI GDGCHC 2 cut(s) 150, 816
Sfr303I CCGCGG 1 cut(s) 969
SfuI TTCGAA 1 cut(s) 836
SgrBI CCGCGG 1 cut(s) 969
SmiMI CAYNNNNRTG 1 cut(s) 469
SmlI CTYRAG 2 cut(s) 584, 1324
SmoI CTYRAG 2 cut(s) 584, 1324
Sse9I AATT 3 cut(s) 58, 189, 1282
SsiI CCGC 4 cut(s) 357, 728, 966, 968
SspMI CTAG 2 cut(s) 1176, 1302
SstI GAGCTC 1 cut(s) 816
StyD4I CCNGG 2 cut(s) 529, 1204
StyI CCWWGG 2 cut(s) 887, 1043
TaaI ACNGT 5 cut(s) 28, 237, 602, 1074, 1200
TaiI ACGT 1 cut(s) 394
TaqI TCGA 4 cut(s) 232, 816, 825, 836
TaqII GACCGA 1 cut(s) 1069
TasI AATT 3 cut(s) 58, 189, 1282
TatI WGTACW 1 cut(s) 765
TauI GCSGC 1 cut(s) 968
TfiI GAWTC 3 cut(s) 164, 242, 526
Tru1I TTAA 9 cut(s) 57, 111, 315, 629, 879, 1034, 1211, 1221, 1281
Tru9I TTAA 9 cut(s) 57, 111, 315, 629, 879, 1034, 1211, 1221, 1281
TscAI CASTG 2 cut(s) 31, 657
TseFI GTSAC 3 cut(s) 498, 709, 970
TseI GCWGC 3 cut(s) 338, 874, 1320
Tsp45I GTSAC 3 cut(s) 498, 709, 970
TspDTI ATGAA 3 cut(s) 177, 567, 966
TspRI CASTG 2 cut(s) 31, 657
VspI ATTAAT 1 cut(s) 1281
XmiI GTMKAC 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 1176, 1302
ZrmI AGTACT 1 cut(s) 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.