MD02G1297300.v1.1

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010030
Physical Location & Seq
Forward (+)
35250539 .. 35253906
3368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1297300.v1.1.491

Sequence Viewer

Length: 1425 bp
ATGGCAAAGAAGAGCTGGCTCAGTATAGTAAAGAGGTTCTTTGTTTCAGACGCACAATCCAAGCAAAAAAATGAGAAGAGAAGGAGATGGATTTTTGGAAAGCTTAAGATCAAAAGACGACCCTCACTAACAGCACCTTCACCAACATCGCCGTCGAAAGAGAGAACACGAAACGAGGCAGATGAAGAACAGAGCAAGCGTGCAATGTCTGTGGCTATGGCGAGCACTGCTGCTGCAGAAGCAGCTGTTGCAGCAGCTCATGCTGCTGTTGAGGTTGTACGGCTCACCGGCACCCAACCATCTTCTTCTGACCAATGTTTGGAGGAGGAGGCAAAAGTGTCGTCAGTCACTACAGCTCAAAGAAAGGCAACTGAATTGGCACATCAATGTGAAAGGGTACAAGAATCTGCTGTTGTCAAAATACAAACTGCCTTCAGAGGTTACCTTGCAAGGAAAGCTTTGAGAGCACTGAAGGGTATAGTGAAGCTACAAGCCATTATCCGAGGATGGGCTGTGAGACGCCAAGCAATGACCACGCTCAAGTGCTTGCAGTCCATAATAAACATCCAATCGCATGCCTGCGCAAGGCGACTCCAAACCATTGAAGACGCTTACTACGGTGATGAACACAATGAGTTCGAAAACTTGAGAGACAAGATAATAAGGATGGATTCAAACAGCCAAAGAAGGTGGGACGACAGCGTTATTTCAAAGAAAGAGGCAGAGGCCATGTTTCTATGCAAGAAAGAAGCTATGCTCAAGAGAGAACGGATAAGGGAATACTCGTATAGTCATCGGAAGTCAGCAGAATCAGAGAGAAACAAGGCAAACGGAAGATGGAGATACTGGTTGGACCAATGGGTGGATACACAACTTTCTAAAAGTAAAGAGCTTGAGGACTTGGACACAGTTTTTGCTTCAAACGCAAGGCGAAAAGAGGAATTTGGAGAAAAACAACTTAGGATGAGAAACTTTCAGAAACAAAATATTCAGATCGAAGGAATGGATTCTCCGGTTTTTGTTCCAAGAAGATCTTCGTACCACAAAAAGCAGTGCTCGTTAGGGGATGAGAATTTGTTCGCAAGCTCTCCTGTTGTTCCAACTTATATGGCTGCAACAAAATCTGCCAAGGCAAAAACAAGGTCTCTCAGTTCCCCCAAGGTAAGGGCAGGGAATTTTGATACTTGCTCAGAAAGCTACTCGCCATGCAAGAAGAACATCTCTCTTATATCTTCTGTAGTCAGTGAAATGCCGATAAGCAGTACTACCGCACGAAAAATGGGAAAGTCCAGTAGTCTCCAGCAACGATCTCCGAGCTTGAGGGGCCTTGCAAGTCCTATAAAATCAAGGCAGACTCCGAAGGATCGAAGCTTCGACTCAGAGTGCTCAATCTCATATTGGAATAGACAAAGCACATGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

475

Amino Acids

53.89

Weight (kDa)

10.0

Isoelectric Point (pI)

74.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 349 - 434 2e-10 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 290
AccB7I CCANNNNNTGG 2 cut(s) 319, 862
AciI CCGC 1 cut(s) 1269
AclWI GGATC 1 cut(s) 1371
AcsI RAATTY 3 cut(s) 941, 1072, 1174
AcuI CTGAAG 2 cut(s) 418, 491
AcyI GRCGYC 1 cut(s) 520
AfaI GTAC 4 cut(s) 279, 399, 1040, 1264
AfiI CCNNNNNNNGG 5 cut(s) 319, 508, 585, 862, 1164
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 4 cut(s) 605, 675, 711, 921
AjuI GAANNNNNNNTTGG 2 cut(s) 1018, 1050
Alw21I GWGCWC 4 cut(s) 227, 469, 1058, 1388
Alw26I GTCTC 4 cut(s) 511, 645, 1149, 1301
AlwI GGATC 1 cut(s) 1371
AoxI GGCC 2 cut(s) 726, 1324
ApeKI GCWGC 7 cut(s) 230, 233, 242, 251, 254, 263, 1112
ApoI RAATTY 3 cut(s) 941, 1072, 1174
ArsI GACNNNNNNTTYG 6 cut(s) 326, 358, 896, 928, 1127, 1159
Asp700I GAANNNNTTC 3 cut(s) 1006, 1033, 1076
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 2 cut(s) 853, 1324
AsuHPI GGTGA 3 cut(s) 132, 277, 632
AsuII TTCGAA 1 cut(s) 639
AvaII GGWCC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 4 cut(s) 227, 469, 1058, 1388
BbvI GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1099
BccI CCATC 5 cut(s) 81, 307, 501, 661, 831
BceAI ACGGC 2 cut(s) 136, 296
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BciVI GTATCC 1 cut(s) 859
BcoDI GTCTC 4 cut(s) 511, 645, 1149, 1301
BfmI CTRYAG 3 cut(s) 234, 351, 1236
BfrI CTTAAG 1 cut(s) 104
BfuI GTATCC 1 cut(s) 859
BglII AGATCT 1 cut(s) 1031
BisI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1113
BlsI GCNGC 7 cut(s) 232, 235, 244, 253, 256, 265, 1114
BmcAI AGTACT 1 cut(s) 1264
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 2 cut(s) 853, 1324
BmiI GGNNCC 2 cut(s) 292, 1325
BpiI GAAGAC 1 cut(s) 612
BpmI CTGGAG 1 cut(s) 1283
Bpu14I TTCGAA 1 cut(s) 639
BpuEI CTTGAG 5 cut(s) 524, 667, 743, 914, 1339
BsaHI GRCGYC 1 cut(s) 520
BsaI GGTCTC 1 cut(s) 1149
BsaJI CCNNGG 3 cut(s) 502, 1128, 1158
BsaWI WCCGGW 1 cut(s) 1012
Bsc4I CCNNNNNNNGG 5 cut(s) 319, 508, 585, 862, 1164
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 851, 1290
Bse3DI GCAATG 2 cut(s) 210, 534
BseDI CCNNGG 3 cut(s) 502, 1128, 1158
BseGI GGATG 5 cut(s) 512, 564, 672, 969, 1072
BseLI CCNNNNNNNGG 5 cut(s) 319, 508, 585, 862, 1164
BseMI GCAATG 2 cut(s) 210, 534
BseMII CTCAG 4 cut(s) 34, 1162, 1203, 1392
BseNI ACTGG 2 cut(s) 851, 1290
BseRI GAGGAG 2 cut(s) 338, 341
BseXI GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1099
BshFI GGCC 2 cut(s) 728, 1326
BshNI GGYRCC 1 cut(s) 290
BsiHKAI GWGCWC 4 cut(s) 227, 469, 1058, 1388
BsiSI CCGG 2 cut(s) 288, 1013
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 5 cut(s) 319, 508, 585, 862, 1164
BsmAI GTCTC 4 cut(s) 511, 645, 1149, 1301
BsmBI CGTCTC 1 cut(s) 511
BsmFI GGGAC 1 cut(s) 707
BsnI GGCC 2 cut(s) 728, 1326
Bso31I GGTCTC 1 cut(s) 1149
Bsp119I TTCGAA 1 cut(s) 639
Bsp1286I GDGCHC 4 cut(s) 227, 469, 1058, 1388
Bsp143I GATC 5 cut(s) 108, 993, 1031, 1307, 1363
BspACI CCGC 1 cut(s) 1269
BspANI GGCC 2 cut(s) 728, 1326
BspCNI CTCAG 4 cut(s) 33, 1161, 1202, 1391
BspLI GGNNCC 2 cut(s) 292, 1325
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 1 cut(s) 1371
BspQI GCTCTTC 1 cut(s) 5
BspT104I TTCGAA 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 290
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 1149
BsrDI GCAATG 2 cut(s) 210, 534
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 851, 1290
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 3 cut(s) 502, 1128, 1158
BssMI GATC 5 cut(s) 108, 993, 1031, 1307, 1363
BssNI GRCGYC 1 cut(s) 520
BssT1I CCWWGG 2 cut(s) 1128, 1158
Bst4CI ACNGT 2 cut(s) 620, 910
Bst6I CTCTTC 2 cut(s) 5, 71
BstACI GRCGYC 1 cut(s) 520
BstAFI CTTAAG 1 cut(s) 104
BstAPI GCANNNNNTGC 2 cut(s) 248, 260
BstBI TTCGAA 1 cut(s) 639
BstC8I GCNNGC 8 cut(s) 17, 197, 201, 223, 548, 576, 580, 1084
BstDEI CTNAG 5 cut(s) 20, 959, 1148, 1189, 1378
BstEII GGTNACC 1 cut(s) 440
BstENI CCTNNNNNAGG 1 cut(s) 583
BstF5I GGATG 5 cut(s) 512, 564, 672, 969, 1072
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 5 cut(s) 111, 996, 1034, 1310, 1366
BstMAI GTCTC 4 cut(s) 511, 645, 1149, 1301
BstMBI GATC 5 cut(s) 108, 993, 1031, 1307, 1363
BstNSI RCATGY 2 cut(s) 578, 1419
BstPI GGTNACC 1 cut(s) 440
BstSFI CTRYAG 3 cut(s) 234, 351, 1236
BstV1I GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1099
BstV2I GAAGAC 1 cut(s) 612
BstX2I RGATCY 1 cut(s) 1031
BstYI RGATCY 1 cut(s) 1031
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 2 cut(s) 728, 1326
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 5 cut(s) 512, 564, 672, 969, 1072
BtsI GCAGTG 2 cut(s) 225, 1058
BtsIMutI CAGTG 4 cut(s) 225, 467, 1058, 1249
Cac8I GCNNGC 8 cut(s) 17, 197, 201, 223, 548, 576, 580, 1084
CfoI GCGC 1 cut(s) 584
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 853, 1324
CseI GACGC 3 cut(s) 59, 528, 617
Csp6I GTAC 4 cut(s) 278, 398, 1039, 1263
CspCI CAANNNNNGTGG 4 cut(s) 192, 227, 671, 706
CviAII CATG 5 cut(s) 260, 575, 730, 1206, 1416
CviQI GTAC 4 cut(s) 278, 398, 1039, 1263
DdeI CTNAG 5 cut(s) 20, 959, 1148, 1189, 1378
DpnI GATC 5 cut(s) 110, 995, 1033, 1309, 1365
DpnII GATC 5 cut(s) 108, 993, 1031, 1307, 1363
Eam1104I CTCTTC 2 cut(s) 5, 71
EarI CTCTTC 2 cut(s) 5, 71
Eco130I CCWWGG 2 cut(s) 1128, 1158
Eco31I GGTCTC 1 cut(s) 1149
Eco47I GGWCC 1 cut(s) 853
Eco57I CTGAAG 2 cut(s) 418, 491
Eco91I GGTNACC 1 cut(s) 440
EcoNI CCTNNNNNAGG 1 cut(s) 583
EcoO109I RGGNCCY 1 cut(s) 1324
EcoO65I GGTNACC 1 cut(s) 440
EcoT14I CCWWGG 2 cut(s) 1128, 1158
ErhI CCWWGG 2 cut(s) 1128, 1158
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 5 cut(s) 263, 578, 733, 1209, 1419
FalI AAGNNNNNCTT 6 cut(s) 23, 55, 442, 474, 1018, 1050
FaqI GGGAC 1 cut(s) 707
FatI CATG 5 cut(s) 259, 574, 729, 1205, 1415
Fnu4HI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1113
FokI GGATG 5 cut(s) 519, 551, 679, 976, 1079
Fsp4HI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1113
FspI TGCGCA 1 cut(s) 583
GlaI GCGC 1 cut(s) 583
GluI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1113
GsuI CTGGAG 1 cut(s) 1283
HaeIII GGCC 2 cut(s) 728, 1326
HapII CCGG 2 cut(s) 288, 1013
HgaI GACGC 3 cut(s) 59, 528, 617
HhaI GCGC 1 cut(s) 584
Hin1I GRCGYC 1 cut(s) 520
Hin1II CATG 5 cut(s) 263, 578, 733, 1209, 1419
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HindIII AAGCTT 3 cut(s) 101, 456, 1369
HinfI GANTC 7 cut(s) 404, 591, 671, 809, 1007, 1354, 1376
HpaII CCGG 2 cut(s) 288, 1013
HphI GGTGA 3 cut(s) 132, 277, 632
Hpy188III TCNNGA 1 cut(s) 760
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 7 cut(s) 75, 147, 442, 466, 681, 992, 1354
HpyCH4III ACNGT 2 cut(s) 620, 910
HpyF3I CTNAG 5 cut(s) 20, 959, 1148, 1189, 1378
Hsp92I GRCGYC 1 cut(s) 520
Hsp92II CATG 5 cut(s) 263, 578, 733, 1209, 1419
HspAI GCGC 1 cut(s) 582
Kzo9I GATC 5 cut(s) 108, 993, 1031, 1307, 1363
LguI GCTCTTC 1 cut(s) 5
LpnPI CCDG 8 cut(s) 301, 592, 832, 1026, 1104, 1155, 1303, 1313
Lsp1109I GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1099
MaeIII GTNAC 2 cut(s) 346, 440
MalI GATC 5 cut(s) 110, 995, 1033, 1309, 1365
MboI GATC 5 cut(s) 108, 993, 1031, 1307, 1363
MflI RGATCY 1 cut(s) 1031
MhlI GDGCHC 4 cut(s) 227, 469, 1058, 1388
MluCI AATT 4 cut(s) 374, 941, 1072, 1174
MlyI GAGTC 3 cut(s) 585, 1348, 1370
MmeI TCCRAC 2 cut(s) 831, 1124
MroXI GAANNNNTTC 3 cut(s) 1006, 1033, 1076
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 3 cut(s) 385, 387, 1420
MspA1I CMGCKG 1 cut(s) 245
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 2 cut(s) 288, 1013
NdeII GATC 5 cut(s) 108, 993, 1031, 1307, 1363
NlaIII CATG 5 cut(s) 263, 578, 733, 1209, 1419
NlaIV GGNNCC 2 cut(s) 292, 1325
NmuCI GTSAC 1 cut(s) 346
NsbI TGCGCA 1 cut(s) 583
NspI RCATGY 2 cut(s) 578, 1419
NspV TTCGAA 1 cut(s) 639
PaeI GCATGC 1 cut(s) 578
PciSI GCTCTTC 1 cut(s) 5
PdmI GAANNNNTTC 3 cut(s) 1006, 1033, 1076
PfeI GAWTC 4 cut(s) 404, 671, 809, 1007
PflMI CCANNNNNTGG 2 cut(s) 319, 862
PkrI GCNGC 7 cut(s) 232, 235, 244, 253, 256, 265, 1114
PleI GAGTC 3 cut(s) 585, 1348, 1370
PpsI GAGTC 3 cut(s) 585, 1348, 1370
PspEI GGTNACC 1 cut(s) 440
PspN4I GGNNCC 2 cut(s) 292, 1325
PspPI GGNCC 2 cut(s) 853, 1324
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 1031
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 4 cut(s) 279, 399, 1040, 1264
RsaNI GTAC 4 cut(s) 278, 398, 1039, 1263
RseI CAYNNNNRTG 3 cut(s) 385, 387, 1420
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1113
Sau3AI GATC 5 cut(s) 108, 993, 1031, 1307, 1363
Sau96I GGNCC 2 cut(s) 853, 1324
ScaI AGTACT 1 cut(s) 1264
SchI GAGTC 3 cut(s) 585, 1348, 1370
SduI GDGCHC 4 cut(s) 227, 469, 1058, 1388
SfcI CTRYAG 3 cut(s) 234, 351, 1236
SfuI TTCGAA 1 cut(s) 639
SinI GGWCC 1 cut(s) 853
SmiMI CAYNNNNRTG 3 cut(s) 385, 387, 1420
SmlI CTYRAG 6 cut(s) 104, 539, 646, 758, 893, 1318
SmoI CTYRAG 6 cut(s) 104, 539, 646, 758, 893, 1318
SphI GCATGC 1 cut(s) 578
Sse9I AATT 4 cut(s) 374, 941, 1072, 1174
SsiI CCGC 1 cut(s) 1269
SspI AATATT 1 cut(s) 988
StyI CCWWGG 2 cut(s) 1128, 1158
TaaI ACNGT 2 cut(s) 620, 910
TaqI TCGA 5 cut(s) 155, 639, 996, 1366, 1374
TasI AATT 4 cut(s) 374, 941, 1072, 1174
TatI WGTACW 1 cut(s) 1262
TfiI GAWTC 4 cut(s) 404, 671, 809, 1007
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 4 cut(s) 232, 474, 1058, 1249
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 7 cut(s) 230, 233, 242, 251, 254, 263, 1112
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 2 cut(s) 198, 639
TspGWI ACGGA 2 cut(s) 784, 846
TspRI CASTG 4 cut(s) 232, 474, 1058, 1249
Van91I CCANNNNNTGG 2 cut(s) 319, 862
Vha464I CTTAAG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 853
XagI CCTNNNNNAGG 1 cut(s) 583
XapI RAATTY 3 cut(s) 941, 1072, 1174
XceI RCATGY 2 cut(s) 578, 1419
XmnI GAANNNNTTC 3 cut(s) 1006, 1033, 1076
ZrmI AGTACT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.