FvH4_7g02140

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010030
Physical Location & Seq
Forward (+)
2479653 .. 2482593
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02140.t2

Sequence Viewer

Length: 1365 bp
ATGGCAAAGAAGAGCTGGTTCAGTATAGTGAAAAGGTTCTTTGTTTCAGACACACAGTCGAAGCAAAATAGTAAGGAGAAGAGAAGAAGATGGGTTTTCGGAAGGCTTAAGATTAAAAGGATACCCTCAATAACAGCACCTGCACCACCATCACCGTTAAAGGAAAAGACACTCAATGAGGCAGAGGAAGAACAAAGTAAGCATGCTCTGAATGTGGCCATTGCGAGCACTGCTGCTGCTGAAGCTGCTGTTGCAGCTGCTCATGCTGCTGCTGAGGTTGTGTGGCTAACTGGTCTTAAATCTCGAAATGAGGCCACTGAATTGGAACATCAATGTGAGAGGGAAACACAAGAACTTGCTGCTGTTAAAATTCAAACCGCCTTTCGTGGTTACCTTGCAAAGAAAGCTTTGAGGGCATTAAAGGGAATAGTGAAGCTCCAAGCTATTATTCGAGGACGAGCTGTGAGACGACAGGCAATGACTACACTCAAGTGCTTGCAGTCCATAATAAACATCCAATCGCACGTATGTGCCAGGAGATTCCAAACAAAAGAAGGAGATACTTATCACTATGATGAATATGATGATCAGTTCAAAAATTTGAGGGATAAGATAATAAGGATGGACTCAAACAGCCAAAGAAGGTGGGATGACAGACTTGTTTCAAAGGAAGAGGCAGAGGCCTTGTGTTTGAGCAAGAAAGAGGCTATGATTAAGAGAGAAAGAATAAAGGAATACTGGTATAGCCATAGGAAATCAGCAGAATCAGAAAGAAAAAAAGTAAATGGAAGATGGAGATACTGGTTGGACCAATGGGTTGACACCCAAATTTCTAAAAGCAAAGAACTTGAAGACTTGGACACAGTTTTGGCTGCGAAAAGAAAAGAGGAGTTTGGAGGTGGAAAACAAGATAGGCTTAGAAACCTACAAAGACAGAATAGTCATCTGGTTAATGAAGGAATGGATTCTCCAAGAAAGTCATCCTTACACCACAGAAAGCAGTGTTCATTGGGAGATGAAACCTCCTTCAAAAGATCTCCTGTTGTTCCTACTTACATGGCTGCAACAAAATCTGCTAAATTGAAAGCGAGGTCATTAAGCTCACCTAAATTAAGGCCATTGAGTTTGGATACTTGCTCCGAGAGCTATTCACCATGCAAGAACAACATCTCTCTTATATCTTCCATCAACAGTGAAGTGCAACTAAGTTCCAGAAGGATCGGCAAGTCCGGTGGTATCCAGCAACGATCTCCAAGCATGAAGGGCTTGCCAAGTCCTGTAAAATCTAGCCAGACCATGAAGGATCGTAGCATTAATTCAGAGTGCTCTATATCATATTGGGATAGTAAGGGTACTTACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

455

Amino Acids

51.85

Weight (kDa)

9.95

Isoelectric Point (pI)

61.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 331 - 403 6.9e-09 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 148
AasI GACNNNNNNGTC 1 cut(s) 939
Acc36I ACCTGC 1 cut(s) 148
AciI CCGC 1 cut(s) 378
AclWI GGATC 2 cut(s) 1226, 1311
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 3 cut(s) 369, 598, 828
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 1 cut(s) 1354
AflII CTTAAG 1 cut(s) 107
AgsI TTSAA 6 cut(s) 374, 595, 666, 851, 1030, 1084
AhdI GACNNNNNGTC 1 cut(s) 55
AjnI CCWGG 1 cut(s) 533
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AleI CACNNNNGTG 2 cut(s) 490, 528
AluBI AGCT 9 cut(s) 15, 245, 257, 407, 436, 443, 461, 1101, 1146
AluI AGCT 9 cut(s) 15, 245, 257, 407, 436, 443, 461, 1101, 1146
Alw21I GWGCWC 2 cut(s) 230, 1328
Alw26I GTCTC 1 cut(s) 460
AlwI GGATC 2 cut(s) 1226, 1311
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 4 cut(s) 216, 312, 681, 1115
ApoI RAATTY 3 cut(s) 369, 598, 828
AseI ATTAAT 1 cut(s) 1314
Asp700I GAANNNNTTC 2 cut(s) 35, 964
AspS9I GGNCC 1 cut(s) 808
AsuHPI GGTGA 3 cut(s) 144, 1095, 1143
AvaII GGWCC 1 cut(s) 808
BalI TGGCCA 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 858
Bbv12I GWGCWC 2 cut(s) 230, 1328
BbvCI CCTCAGC 1 cut(s) 273
BccI CCATC 5 cut(s) 84, 157, 616, 786, 1193
BciT130I CCWGG 1 cut(s) 535
BciVI GTATCC 3 cut(s) 114, 1123, 1247
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 460
BfaI CTAG 1 cut(s) 1287
BfrI CTTAAG 1 cut(s) 107
BfuAI ACCTGC 1 cut(s) 148
BfuI GTATCC 3 cut(s) 114, 1123, 1247
BglII AGATCT 1 cut(s) 1034
Bme1390I CCNGG 1 cut(s) 535
Bme18I GGWCC 1 cut(s) 808
BmeRI GACNNNNNGTC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 808
BmrFI CCNGG 1 cut(s) 535
BpiI GAAGAC 1 cut(s) 858
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 473
BsaAI YACGTR 1 cut(s) 526
BsaBI GATNNNNATC 1 cut(s) 564
BsaWI WCCGGW 1 cut(s) 1229
Bse1I ACTGG 3 cut(s) 295, 743, 806
Bse3DI GCAATG 2 cut(s) 219, 483
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 1 cut(s) 535
BseGI GGATG 4 cut(s) 513, 627, 655, 980
BseJI GATNNNNATC 1 cut(s) 564
BseMI GCAATG 2 cut(s) 219, 483
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 3 cut(s) 295, 743, 806
BseRI GAGGAG 1 cut(s) 902
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 4 cut(s) 218, 314, 683, 1117
BsiHKAI GWGCWC 2 cut(s) 230, 1328
BsiSI CCGG 1 cut(s) 1230
BsmAI GTCTC 1 cut(s) 460
BsmBI CGTCTC 1 cut(s) 460
BsnI GGCC 4 cut(s) 218, 314, 683, 1117
Bsp1286I GDGCHC 2 cut(s) 230, 1328
Bsp143I GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
BspACI CCGC 1 cut(s) 378
BspANI GGCC 4 cut(s) 218, 314, 683, 1117
BspCNI CTCAG 1 cut(s) 265
BspMI ACCTGC 1 cut(s) 148
BspPI GGATC 2 cut(s) 1226, 1311
BspQI GCTCTTC 1 cut(s) 5
BspTI CTTAAG 1 cut(s) 107
BsrDI GCAATG 2 cut(s) 219, 483
BsrI ACTGG 3 cut(s) 295, 743, 806
BssMI GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
Bst2UI CCWGG 1 cut(s) 535
Bst4CI ACNGT 4 cut(s) 57, 156, 865, 1193
Bst6I CTCTTC 3 cut(s) 5, 74, 666
BstAFI CTTAAG 1 cut(s) 107
BstBAI YACGTR 1 cut(s) 526
BstC8I GCNNGC 4 cut(s) 204, 226, 497, 1268
BstDEI CTNAG 3 cut(s) 273, 917, 1205
BstEII GGTNACC 1 cut(s) 389
BstF5I GGATG 4 cut(s) 513, 627, 655, 980
BstKTI GATC 5 cut(s) 589, 1037, 1221, 1250, 1306
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
BstNI CCWGG 1 cut(s) 535
BstNSI RCATGY 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 1 cut(s) 1034
BstXI CCANNNNNNTGG 1 cut(s) 322
BstYI RGATCY 1 cut(s) 1034
BsuI GTATCC 3 cut(s) 114, 1123, 1247
BsuRI GGCC 4 cut(s) 218, 314, 683, 1117
BtsCI GGATG 4 cut(s) 513, 627, 655, 980
BtsI GCAGTG 2 cut(s) 228, 1007
BtsIMutI CAGTG 4 cut(s) 228, 315, 1007, 1198
BveI ACCTGC 1 cut(s) 148
Cac8I GCNNGC 4 cut(s) 204, 226, 497, 1268
CaiI CAGNNNCTG 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 808
Csp6I GTAC 1 cut(s) 1353
CspCI CAANNNNNGTGG 4 cut(s) 626, 661, 1213, 1248
CviAII CATG 6 cut(s) 203, 263, 1057, 1155, 1258, 1297
CviQI GTAC 1 cut(s) 1353
DdeI CTNAG 3 cut(s) 273, 917, 1205
DpnI GATC 5 cut(s) 588, 1036, 1220, 1249, 1305
DpnII GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
DrdI GACNNNNNNGTC 1 cut(s) 939
DriI GACNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 939
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 3 cut(s) 5, 74, 666
Eam1105I GACNNNNNGTC 1 cut(s) 55
EarI CTCTTC 3 cut(s) 5, 74, 666
Eco147I AGGCCT 1 cut(s) 683
Eco47I GGWCC 1 cut(s) 808
Eco57I CTGAAG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 389
EcoO65I GGTNACC 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 533
Esp3I CGTCTC 1 cut(s) 460
FaeI CATG 6 cut(s) 206, 266, 1060, 1158, 1261, 1300
FalI AAGNNNNNCTT 4 cut(s) 900, 932, 968, 1000
FatI CATG 6 cut(s) 202, 262, 1056, 1154, 1257, 1296
FbaI TGATCA 1 cut(s) 586
FokI GGATG 4 cut(s) 500, 634, 662, 967
FspBI CTAG 1 cut(s) 1287
HaeIII GGCC 4 cut(s) 218, 314, 683, 1117
HapII CCGG 1 cut(s) 1230
Hin1II CATG 6 cut(s) 206, 266, 1060, 1158, 1261, 1300
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 4 cut(s) 540, 626, 764, 965
HpaII CCGG 1 cut(s) 1230
HphI GGTGA 3 cut(s) 144, 1095, 1143
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 6 cut(s) 49, 101, 210, 769, 1141, 1321
Hpy188III TCNNGA 2 cut(s) 303, 1212
Hpy8I GTNNAC 1 cut(s) 820
HpyAV CCTTC 8 cut(s) 96, 548, 636, 950, 1036, 1209, 1255, 1294
HpyCH4III ACNGT 4 cut(s) 57, 156, 865, 1193
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 7 cut(s) 143, 254, 398, 499, 1064, 1158, 1201
HpyF3I CTNAG 3 cut(s) 273, 917, 1205
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 6 cut(s) 206, 266, 1060, 1158, 1261, 1300
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
LguI GCTCTTC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 441, 1142
MaeI CTAG 1 cut(s) 1287
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 1 cut(s) 389
MalI GATC 5 cut(s) 588, 1036, 1220, 1249, 1305
MboI GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
MboII GAAGA 9 cut(s) 22, 91, 96, 99, 200, 683, 801, 863, 1173
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 2 cut(s) 230, 1328
MlsI TGGCCA 1 cut(s) 218
MluCI AATT 7 cut(s) 320, 369, 598, 828, 1079, 1109, 1315
MluNI TGGCCA 1 cut(s) 218
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 1 cut(s) 786
Mox20I TGGCCA 1 cut(s) 218
MroXI GAANNNNTTC 2 cut(s) 35, 964
MscI TGGCCA 1 cut(s) 218
MslI CAYNNNNRTG 4 cut(s) 333, 490, 528, 573
Msp20I TGGCCA 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 257
MspCI CTTAAG 1 cut(s) 107
MspI CCGG 1 cut(s) 1230
MspR9I CCNGG 1 cut(s) 535
MvaI CCWGG 1 cut(s) 535
NdeII GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
NlaIII CATG 6 cut(s) 206, 266, 1060, 1158, 1261, 1300
NspI RCATGY 1 cut(s) 206
OliI CACNNNNGTG 2 cut(s) 490, 528
PaeI GCATGC 1 cut(s) 206
PaqCI CACCTGC 1 cut(s) 148
PceI AGGCCT 1 cut(s) 683
PciSI GCTCTTC 1 cut(s) 5
PdmI GAANNNNTTC 2 cut(s) 35, 964
PfeI GAWTC 3 cut(s) 540, 764, 965
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
Ppu21I YACGTR 1 cut(s) 526
PshBI ATTAAT 1 cut(s) 1314
Psp6I CCWGG 1 cut(s) 533
PspEI GGTNACC 1 cut(s) 389
PspGI CCWGG 1 cut(s) 533
PspPI GGNCC 1 cut(s) 808
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 1 cut(s) 1354
RsaNI GTAC 1 cut(s) 1353
RseI CAYNNNNRTG 4 cut(s) 333, 490, 528, 573
SapI GCTCTTC 1 cut(s) 5
Sau3AI GATC 5 cut(s) 586, 1034, 1218, 1247, 1303
Sau96I GGNCC 1 cut(s) 808
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 2 cut(s) 230, 1328
SinI GGWCC 1 cut(s) 808
SmiMI CAYNNNNRTG 4 cut(s) 333, 490, 528, 573
SmlI CTYRAG 2 cut(s) 107, 488
SmoI CTYRAG 2 cut(s) 107, 488
SphI GCATGC 1 cut(s) 206
Sse9I AATT 7 cut(s) 320, 369, 598, 828, 1079, 1109, 1315
SseBI AGGCCT 1 cut(s) 683
SsiI CCGC 1 cut(s) 378
SspMI CTAG 1 cut(s) 1287
StuI AGGCCT 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 533
TaaI ACNGT 4 cut(s) 57, 156, 865, 1193
TaiI ACGT 1 cut(s) 528
TaqI TCGA 3 cut(s) 59, 304, 451
TasI AATT 7 cut(s) 320, 369, 598, 828, 1079, 1109, 1315
TfiI GAWTC 3 cut(s) 540, 764, 965
TscAI CASTG 4 cut(s) 235, 322, 1007, 1198
TspDTI ATGAA 6 cut(s) 591, 969, 996, 1032, 1274, 1313
TspRI CASTG 4 cut(s) 235, 322, 1007, 1198
Vha464I CTTAAG 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 808
VspI ATTAAT 1 cut(s) 1314
XapI RAATTY 3 cut(s) 369, 598, 828
XceI RCATGY 1 cut(s) 206
XmnI GAANNNNTTC 2 cut(s) 35, 964
XspI CTAG 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.