Rorug01G0027000

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010030
Physical Location & Seq
Forward (+)
4605151 .. 4607782
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0027000.1

Sequence Viewer

Length: 1428 bp
ATGTCTGGTGTAAATCATAAGAGTGGTAAAGATCATACAGTTATCGATATTAATGACAAGAACTGCATCTTCAGAGTTCCTAATGTGCTCCGGAGACAAAATCCAGAAGCATATACACCTGACGTTGTTTCAATCGGACCTTTTCATCGTTATCGAGTAAAGCGAGGCAAAGAGGAAGGTAAAGGAGGCAAAGATGACAAAGAAGGCAAAGGTGAGGAAGATTTTCAACTCATAAAACAAGTGAAAAGGAGATATTTGAATGAATTTCTCACAGGTATAAAGAATATAACTTTGGAAGAGTTGACTACAGAAGTCATTAAGCTCTCAGATCAAAAGAATGAAGGCGAATTTGAGCAACGCGCGCGCGATTTTTATGCAGAATCACTTCATATTTCCACTAAAAAGTTCATCAAGATGATGATAGTTGACGGTTGCTTCCTAATTCAACTGTTTCGGAAGTGTGACGATGAGAAACTCAGGGCCTTTGATGACCCGGTGTTCAACATGGACTGTATGTTTCATTTCCTATGCCATGACATTTTGCTCCTAGAAAATCAACTACCTTGGTTTGTTATCCACAGTTTGTATAGCCTTATCTCTAAAGAATACCCTAATCAAGCCTCCCTCTCTTTTCTCGTCCTTGAAGTCTTCAGCACGCTACCATCACTGAAGCATAGTTGCTCAAACTATCAAACGCATCTCCGGACAAAAGAGAGACACTGTGATGCTGATTTTCTGCACATACTTGATCTGGTAAGAGCTTCTATGGTTAATCCATTAACTATCATAGATGTGAGAGCAAATGAAGCAGCTAGAAAACACGAGAGAGGCAATAAAGCTGTTCATGAAGACCCAGATAAAAATCAAATCCGTACCGCAACCGCTCTCTCAAAGGCAGACATTAAATTCAAAAACGTCGAAACGGATAGCAATCATAGCATAATGGACATCCAATTCAAAGCGGGTGGGTGGTTCAACAATGGGATTCTTGAAATTCCACAGCTAAATGTTGGAATGTCATCGAAAACATTATTCAGGAACCTTATTGCAATTGAGCAATGCTACCATGGTTATTCAAACGCGATTACATCTTATGCCATCTTTATGGATAACCTCATCTCTTCAAATGAAGATATGGAATTACTTCGCATGGAAAAAGTAATAGGTAACTGGATGAGTGATGAAGATGGTTGTAAGTTTTTCAGCGACCTTTACAAGGATATCGCGCACAAGAAGTTCTACTATGTTGATCTGTGCGAACAACTGAATTATCGTTACAGATTGAAACGGTATACATGGTTGGCTTCACTCAAGAGTGAAAAATTTTCTAACCCCTGCAGAATTTTGGCTTTCGCGATGGGCATTTTCATTTTGATTCTCACCGCGTGGAGTCAGATAAACAACATTCGGGTTAACATGCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

475

Amino Acids

55.26

Weight (kDa)

7.92

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 23 - 460 9e-98 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 884
AccI GTMKAC 1 cut(s) 1292
AccII CGCG 8 cut(s) 360, 362, 364, 366, 1082, 1226, 1355, 1385
AccIII TCCGGA 2 cut(s) 90, 702
AciI CCGC 4 cut(s) 876, 882, 962, 1383
AcsI RAATTY 6 cut(s) 263, 347, 905, 993, 1322, 1341
AcuI CTGAAG 3 cut(s) 55, 634, 689
AdeI CACNNNGTG 1 cut(s) 1386
AfaI GTAC 1 cut(s) 874
AfiI CCNNNNNNNGG 1 cut(s) 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 5 cut(s) 322, 761, 812, 839, 1003
AluI AGCT 5 cut(s) 322, 761, 812, 839, 1003
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 2 cut(s) 88, 709
Aor13HI TCCGGA 2 cut(s) 90, 702
AoxI GGCC 1 cut(s) 480
ApeKI GCWGC 1 cut(s) 809
ApoI RAATTY 6 cut(s) 263, 347, 905, 993, 1322, 1341
AseI ATTAAT 1 cut(s) 51
Asp700I GAANNNNTTC 3 cut(s) 222, 384, 1143
AspLEI GCGC 4 cut(s) 362, 364, 366, 1228
AspS9I GGNCC 2 cut(s) 137, 480
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 2 cut(s) 224, 1372
AvaII GGWCC 1 cut(s) 137
BauI CACGAG 1 cut(s) 821
BbsI GAAGAC 2 cut(s) 640, 855
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 821
BccI CCATC 4 cut(s) 670, 1106, 1181, 1351
BcgI CGANNNNNNTGC 2 cut(s) 356, 390
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 2 cut(s) 88, 709
BfaI CTAG 2 cut(s) 548, 813
BfmI CTRYAG 2 cut(s) 306, 1336
BisI GCNGC 1 cut(s) 810
BlsI GCNGC 1 cut(s) 811
Bme1390I CCNGG 1 cut(s) 494
Bme18I GGWCC 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 137, 480
BmiI GGNNCC 1 cut(s) 1040
BmrFI CCNGG 1 cut(s) 494
BmsI GCATC 3 cut(s) 75, 706, 715
BpiI GAAGAC 2 cut(s) 640, 855
BpuEI CTTGAG 1 cut(s) 1295
BpuMI CCSGG 1 cut(s) 494
Bsa29I ATCGAT 1 cut(s) 45
BsaBI GATNNNNATC 2 cut(s) 861, 930
BsaJI CCNNGG 2 cut(s) 563, 1066
BsaWI WCCGGW 2 cut(s) 90, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 1216
Bse1I ACTGG 1 cut(s) 1175
Bse3DI GCAATG 1 cut(s) 1064
Bse8I GATNNNNATC 2 cut(s) 861, 930
BseAI TCCGGA 2 cut(s) 90, 702
BseCI ATCGAT 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 563, 1066
BseGI GGATG 2 cut(s) 948, 1179
BseJI GATNNNNATC 2 cut(s) 861, 930
BseLI CCNNNNNNNGG 1 cut(s) 1216
BseMI GCAATG 1 cut(s) 1064
BseMII CTCAG 2 cut(s) 339, 490
BseNI ACTGG 1 cut(s) 1175
BsePI GCGCGC 2 cut(s) 360, 362
BseXI GCAGC 1 cut(s) 821
BsgI GTGCAG 1 cut(s) 722
Bsh1236I CGCG 8 cut(s) 360, 362, 364, 366, 1082, 1226, 1355, 1385
BshFI GGCC 1 cut(s) 482
BshVI ATCGAT 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 3 cut(s) 91, 494, 703
BslI CCNNNNNNNGG 1 cut(s) 1216
BsmAI GTCTC 2 cut(s) 88, 709
BsnI GGCC 1 cut(s) 482
Bsp1286I GDGCHC 1 cut(s) 90
Bsp13I TCCGGA 2 cut(s) 90, 702
Bsp143I GATC 4 cut(s) 31, 328, 748, 1249
Bsp19I CCATGG 1 cut(s) 1066
Bsp68I TCGCGA 1 cut(s) 1355
BspACI CCGC 4 cut(s) 876, 882, 962, 1383
BspANI GGCC 1 cut(s) 482
BspCNI CTCAG 2 cut(s) 338, 489
BspDI ATCGAT 1 cut(s) 45
BspEI TCCGGA 2 cut(s) 90, 702
BspFNI CGCG 8 cut(s) 360, 362, 364, 366, 1082, 1226, 1355, 1385
BspHI TCATGA 1 cut(s) 844
BspLI GGNNCC 1 cut(s) 1040
BspMAI CTGCAG 1 cut(s) 1340
BsrBI CCGCTC 1 cut(s) 884
BsrDI GCAATG 1 cut(s) 1064
BsrI ACTGG 1 cut(s) 1175
BssECI CCNNGG 2 cut(s) 563, 1066
BssHII GCGCGC 2 cut(s) 360, 362
BssMI GATC 4 cut(s) 31, 328, 748, 1249
BssNAI GTATAC 1 cut(s) 1293
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 2 cut(s) 563, 1066
Bst1107I GTATAC 1 cut(s) 1293
Bst2BI CACGAG 1 cut(s) 821
Bst4CI ACNGT 7 cut(s) 40, 431, 450, 512, 581, 722, 1290
Bst6I CTCTTC 2 cut(s) 291, 1126
BstC8I GCNNGC 3 cut(s) 362, 364, 656
BstDEI CTNAG 2 cut(s) 325, 476
BstDSI CCRYGG 1 cut(s) 1066
BstENI CCTNNNNNAGG 1 cut(s) 1214
BstF5I GGATG 2 cut(s) 948, 1179
BstFNI CGCG 8 cut(s) 360, 362, 364, 366, 1082, 1226, 1355, 1385
BstHHI GCGC 4 cut(s) 362, 364, 366, 1228
BstKTI GATC 4 cut(s) 34, 331, 751, 1252
BstMAI GTCTC 2 cut(s) 88, 709
BstMBI GATC 4 cut(s) 31, 328, 748, 1249
BstMWI GCNNNNNNNGC 3 cut(s) 361, 806, 936
BstNSI RCATGY 1 cut(s) 1420
BstSCI CCNGG 1 cut(s) 492
BstSFI CTRYAG 2 cut(s) 306, 1336
BstUI CGCG 8 cut(s) 360, 362, 364, 366, 1082, 1226, 1355, 1385
BstV1I GCAGC 1 cut(s) 821
BstV2I GAAGAC 2 cut(s) 640, 855
BstXI CCANNNNNNTGG 1 cut(s) 1105
BstZ17I GTATAC 1 cut(s) 1293
Bsu15I ATCGAT 1 cut(s) 45
BsuRI GGCC 1 cut(s) 482
BsuTUI ATCGAT 1 cut(s) 45
BtgI CCRYGG 1 cut(s) 1066
BtgZI GCGATG 1 cut(s) 1370
BtsCI GGATG 2 cut(s) 948, 1179
BtsIMutI CAGTG 2 cut(s) 665, 718
BtuMI TCGCGA 1 cut(s) 1355
Cac8I GCNNGC 3 cut(s) 362, 364, 656
CciI TCATGA 1 cut(s) 844
CfoI GCGC 4 cut(s) 362, 364, 366, 1228
Cfr13I GGNCC 2 cut(s) 137, 480
ClaI ATCGAT 1 cut(s) 45
Csp6I GTAC 1 cut(s) 873
CspCI CAANNNNNGTGG 2 cut(s) 946, 981
CviAII CATG 7 cut(s) 505, 533, 845, 1067, 1150, 1296, 1417
CviQI GTAC 1 cut(s) 873
DdeI CTNAG 2 cut(s) 325, 476
DpnI GATC 4 cut(s) 33, 330, 750, 1251
DpnII GATC 4 cut(s) 31, 328, 748, 1249
DraIII CACNNNGTG 1 cut(s) 1386
Eam1104I CTCTTC 2 cut(s) 291, 1126
EarI CTCTTC 2 cut(s) 291, 1126
Eco130I CCWWGG 2 cut(s) 563, 1066
Eco32I GATATC 1 cut(s) 1222
Eco47I GGWCC 1 cut(s) 137
Eco57I CTGAAG 3 cut(s) 55, 634, 689
EcoNI CCTNNNNNAGG 1 cut(s) 1214
EcoO109I RGGNCCY 1 cut(s) 480
EcoRV GATATC 1 cut(s) 1222
EcoT14I CCWWGG 2 cut(s) 563, 1066
ErhI CCWWGG 2 cut(s) 563, 1066
FaeI CATG 7 cut(s) 508, 536, 848, 1070, 1153, 1299, 1420
FatI CATG 7 cut(s) 504, 532, 844, 1066, 1149, 1295, 1416
FauI CCCGC 1 cut(s) 955
FblI GTMKAC 1 cut(s) 1292
Fnu4HI GCNGC 1 cut(s) 810
FokI GGATG 2 cut(s) 935, 1186
Fsp4HI GCNGC 1 cut(s) 810
FspBI CTAG 2 cut(s) 548, 813
GlaI GCGC 4 cut(s) 361, 363, 365, 1227
GluI GCNGC 1 cut(s) 810
HaeIII GGCC 1 cut(s) 482
HapII CCGG 3 cut(s) 91, 494, 703
HhaI GCGC 4 cut(s) 362, 364, 366, 1228
Hin1II CATG 7 cut(s) 508, 536, 848, 1070, 1153, 1299, 1420
Hin6I GCGC 4 cut(s) 360, 362, 364, 1226
HinP1I GCGC 4 cut(s) 360, 362, 364, 1226
HincII GTYRAC 3 cut(s) 303, 427, 1414
HindII GTYRAC 3 cut(s) 303, 427, 1414
HinfI GANTC 4 cut(s) 380, 985, 1375, 1390
HpaI GTTAAC 1 cut(s) 1414
HpaII CCGG 3 cut(s) 91, 494, 703
HphI GGTGA 2 cut(s) 224, 1372
Hpy166II GTNNAC 4 cut(s) 303, 427, 1293, 1414
Hpy188I TCNGA 5 cut(s) 74, 137, 328, 456, 1395
Hpy188III TCNNGA 9 cut(s) 91, 104, 412, 703, 845, 989, 1036, 1312, 1354
Hpy8I GTNNAC 4 cut(s) 303, 427, 1293, 1414
Hpy99I CGWCG 1 cut(s) 920
HpyAV CCTTC 3 cut(s) 170, 197, 335
HpyCH4III ACNGT 7 cut(s) 40, 431, 450, 512, 581, 722, 1290
HpyCH4IV ACGT 2 cut(s) 123, 915
HpyCH4V TGCA 6 cut(s) 66, 377, 739, 1049, 1338, 1420
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 806, 936
HpyF3I CTNAG 2 cut(s) 325, 476
HpySE526I ACGT 2 cut(s) 123, 915
Hsp92II CATG 7 cut(s) 508, 536, 848, 1070, 1153, 1299, 1420
HspAI GCGC 4 cut(s) 360, 362, 364, 1226
Kpn2I TCCGGA 2 cut(s) 90, 702
KspAI GTTAAC 1 cut(s) 1414
Kzo9I GATC 4 cut(s) 31, 328, 748, 1249
LmnI GCTCC 2 cut(s) 93, 549
Lsp1109I GCAGC 1 cut(s) 821
LweI GCATC 3 cut(s) 75, 706, 715
MaeI CTAG 2 cut(s) 548, 813
MaeII ACGT 2 cut(s) 123, 915
MaeIII GTNAC 3 cut(s) 461, 1166, 1274
MalI GATC 4 cut(s) 33, 330, 750, 1251
MauBI CGCGCGCG 2 cut(s) 360, 362
MbiI CCGCTC 1 cut(s) 884
MboI GATC 4 cut(s) 31, 328, 748, 1249
MboII GAAGA 8 cut(s) 61, 230, 308, 640, 860, 1113, 1142, 1196
MfeI CAATTG 1 cut(s) 1050
MhlI GDGCHC 1 cut(s) 90
MlyI GAGTC 1 cut(s) 1399
MmeI TCCRAC 1 cut(s) 991
MnlI CCTC 8 cut(s) 158, 166, 179, 208, 631, 635, 821, 1124
MroI TCCGGA 2 cut(s) 90, 702
MroXI GAANNNNTTC 3 cut(s) 222, 384, 1143
MseI TTAA 6 cut(s) 51, 318, 771, 779, 903, 1413
MslI CAYNNNNRTG 5 cut(s) 21, 413, 723, 791, 1103
MspI CCGG 3 cut(s) 91, 494, 703
MspR9I CCNGG 1 cut(s) 494
MunI CAATTG 1 cut(s) 1050
MvnI CGCG 8 cut(s) 360, 362, 364, 366, 1082, 1226, 1355, 1385
MwoI GCNNNNNNNGC 3 cut(s) 361, 806, 936
NciI CCSGG 1 cut(s) 494
NcoI CCATGG 1 cut(s) 1066
NdeII GATC 4 cut(s) 31, 328, 748, 1249
NlaIII CATG 7 cut(s) 508, 536, 848, 1070, 1153, 1299, 1420
NlaIV GGNNCC 1 cut(s) 1040
NmuCI GTSAC 1 cut(s) 461
NruI TCGCGA 1 cut(s) 1355
NspI RCATGY 1 cut(s) 1420
PagI TCATGA 1 cut(s) 844
PauI GCGCGC 2 cut(s) 360, 362
PcsI WCGNNNNNNNCGW 1 cut(s) 160
PdmI GAANNNNTTC 3 cut(s) 222, 384, 1143
PfeI GAWTC 3 cut(s) 380, 985, 1375
PkrI GCNGC 1 cut(s) 811
PleI GAGTC 1 cut(s) 1398
PpsI GAGTC 1 cut(s) 1398
PshBI ATTAAT 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 1040
PspPI GGNCC 2 cut(s) 137, 480
PstI CTGCAG 1 cut(s) 1340
PteI GCGCGC 2 cut(s) 360, 362
RruI TCGCGA 1 cut(s) 1355
RsaI GTAC 1 cut(s) 874
RsaNI GTAC 1 cut(s) 873
RseI CAYNNNNRTG 5 cut(s) 21, 413, 723, 791, 1103
SaqAI TTAA 6 cut(s) 51, 318, 771, 779, 903, 1413
SatI GCNGC 1 cut(s) 810
Sau3AI GATC 4 cut(s) 31, 328, 748, 1249
Sau96I GGNCC 2 cut(s) 137, 480
SchI GAGTC 1 cut(s) 1399
ScrFI CCNGG 1 cut(s) 494
SduI GDGCHC 1 cut(s) 90
SfaNI GCATC 3 cut(s) 75, 706, 715
SfcI CTRYAG 2 cut(s) 306, 1336
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 5 cut(s) 21, 413, 723, 791, 1103
SmlI CTYRAG 1 cut(s) 1310
SmoI CTYRAG 1 cut(s) 1310
SsiI CCGC 4 cut(s) 876, 882, 962, 1383
SspMI CTAG 2 cut(s) 548, 813
StyD4I CCNGG 1 cut(s) 492
StyI CCWWGG 2 cut(s) 563, 1066
TaaI ACNGT 7 cut(s) 40, 431, 450, 512, 581, 722, 1290
TaiI ACGT 2 cut(s) 126, 918
TaqI TCGA 4 cut(s) 45, 154, 918, 1022
TfiI GAWTC 3 cut(s) 380, 985, 1375
Tru1I TTAA 6 cut(s) 51, 318, 771, 779, 903, 1413
Tru9I TTAA 6 cut(s) 51, 318, 771, 779, 903, 1413
TscAI CASTG 2 cut(s) 672, 725
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 1 cut(s) 809
Tsp45I GTSAC 1 cut(s) 461
TspGWI ACGGA 2 cut(s) 860, 938
TspRI CASTG 2 cut(s) 672, 725
VpaK11BI GGWCC 1 cut(s) 137
VspI ATTAAT 1 cut(s) 51
XagI CCTNNNNNAGG 1 cut(s) 1214
XapI RAATTY 6 cut(s) 263, 347, 905, 993, 1322, 1341
XceI RCATGY 1 cut(s) 1420
XmiI GTMKAC 1 cut(s) 1292
XmnI GAANNNNTTC 3 cut(s) 222, 384, 1143
XspI CTAG 2 cut(s) 548, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.