Rorug01G0027100

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010030
Physical Location & Seq
Forward (+)
4638963 .. 4643194
4232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0027100.1

Sequence Viewer

Length: 1509 bp
ATGAATATTCCCAAGCCTTTTTTGGTTCTTCTCTCTTCCTTTCTCATTCTTTCTTGCTTCTCCATAGCTCAAATCCATGGGAGCAAGCATTCACAAGCGCTTTCTCAGCTGTACAAGTCCAAGCTGAATTCCGAAAGTGCAGGCATCGACACGAAGCCATTCGAGGCGATTCTTCATGCGAACGCAACCAGCATTCATCCCCAAGAAGGGTTGAAGGAGAAAGATCGGATCAAAAGCTTGCCTGGACAACCCAAGGTGAAGTTCAGTCAGTATGGTGGGTATGTTACTGTGGATAAAAAAGCAGGTCGTGCACTTTTTTACTACTTTGCTGAAGCTGACAATCAGCAGGCCAAGGATTCTTTGCCTCTTCTTCTATGGCTCAATGGAGGACCTGGATGTTCCTCTCTTGCCTATGGAGCAATGCTTGAGCTCGGACCATTCCGTATCCATAGCGATGGCAAAACCCTTTACAGAAATAGATTTTCATGGAACTATGCTGCAAATGTTTTGTTCCTTGAGTCTCCTGCTGGTGTGGGTTTTTCATACTCTAACATAACATCAGATTATGATAAAAGTGGGGACAGAAGAACAGCTGCAGATAATTATGTGTTCTTAGTGAATTGGTTGGAGAGGTTTCCTGAATATAAGGGGAGGGAGTTCTATATTGCTGGTGAAAGCTATGCAGGGCACTATGTACCTCAACTTGCACAGACTATCCTCCACCACAACAAGAAGGCTAATAGAACCATCATCAACCTCAAGGGCATAAATATTGGGAATGCAGTGATTAATGATGAAACTGATACAAGAGGGATGTATGACTACCTTGCTTCCCATGCTCTCATTTCCGATGAAATTGCAAATCAAGTCCAAAAATACTGTGACTTGTCACCCAATGCCACTACTCAACCCAAAGAGTGCGATGACGCTTCTGCTGCTATTGAGAAGGCTACCTATTTCATTAATATCTATAACATCTATGCCCCCTCATGTGCCTCTTCCAATCTCACTGTCCAACCCAAAAAGTCTTCAATACTGAAATTTGATCCGTGTAGCGAGAACTATGCGTATGCTTATTTGAATCGGCCTGATGTTCAAGAAGCTCTCCACGCTAATGTCACTAAACTCACACATGACTGGGAACCATGTAGTGATGTAATCACACACTGGGCAGATAGCCCATCAACCATACTACCCCTTTTACAAGAGTTTATGGATAATGGACTTCGAGTCTGGATTTTTAGTGGCGATATCGATGGAAGGGTTCCAGTCACTTCAACAAAGTATTCTCTTGAGAAGATGAATCTACCAATCAAGACCGAATGGCACGCTTGGTTCCTTAGCGGACAAGTTGGTGGTTACACACAAGTGTATGAAGGAGACATAACACTTGCCACAGTGAGGGAGGCAGGGCATCAAGTCCCAAGCTACCAGCCTGCTAGGGCACTTTCACTCATCAAGCACTTCCTTGATGGCACACCTCTTCCTGATACTAAAAGATATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

502

Amino Acids

56.14

Weight (kDa)

6.57

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 81 - 492 1.7e-142 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 293
AciI CCGC 1 cut(s) 1344
AclWI GGATC 2 cut(s) 236, 1040
AcsI RAATTY 2 cut(s) 127, 1040
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 2 cut(s) 113, 696
AfeI AGCGCT 1 cut(s) 99
AfiI CCNNNNNNNGG 3 cut(s) 206, 207, 1401
AgsI TTSAA 5 cut(s) 214, 1032, 1081, 1097, 1278
AhdI GACNNNNNGTC 1 cut(s) 1229
AjnI CCWGG 2 cut(s) 241, 391
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AleI CACNNNNGTG 1 cut(s) 1367
Alw21I GWGCWC 2 cut(s) 313, 432
Alw26I GTCTC 2 cut(s) 525, 1374
Alw44I GTGCAC 1 cut(s) 309
AlwI GGATC 2 cut(s) 236, 1040
Aor51HI AGCGCT 1 cut(s) 99
AoxI GGCC 2 cut(s) 348, 1085
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 497, 593, 935
ApoI RAATTY 2 cut(s) 127, 1040
AseI ATTAAT 2 cut(s) 789, 963
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 389, 434
AsuHPI GGTGA 3 cut(s) 268, 683, 882
AvaII GGWCC 2 cut(s) 389, 434
BaeGI GKGCMC 3 cut(s) 313, 690, 1447
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 1020
Bbv12I GWGCWC 2 cut(s) 313, 432
BbvI GCAGC 3 cut(s) 484, 580, 922
BccI CCATC 5 cut(s) 449, 755, 1189, 1250, 1466
BcgI CGANNNNNNTGC 4 cut(s) 839, 873, 1046, 1080
BciT130I CCWGG 2 cut(s) 243, 393
BciVI GTATCC 1 cut(s) 455
BcoDI GTCTC 2 cut(s) 525, 1374
BfaI CTAG 1 cut(s) 1440
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 455
BisI GCNGC 3 cut(s) 498, 594, 936
BlsI GCNGC 3 cut(s) 499, 595, 937
Bme1390I CCNGG 2 cut(s) 243, 393
Bme18I GGWCC 2 cut(s) 389, 434
BmeRI GACNNNNNGTC 1 cut(s) 1229
BmgT120I GGNCC 2 cut(s) 389, 434
BmiI GGNNCC 3 cut(s) 1143, 1266, 1337
BmrFI CCNGG 2 cut(s) 243, 393
BmrI ACTGGG 2 cut(s) 1147, 1177
BmsI GCATC 2 cut(s) 153, 1423
BmuI ACTGGG 2 cut(s) 1147, 1177
BpiI GAAGAC 1 cut(s) 1020
Bpu10I CCTNAGC 1 cut(s) 1340
BpuEI CTTGAG 4 cut(s) 446, 536, 743, 1313
Bsa29I ATCGAT 1 cut(s) 1254
BsaJI CCNNGG 3 cut(s) 76, 252, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 207, 1401
Bse1I ACTGG 3 cut(s) 1142, 1172, 1268
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 243, 393
BseCI ATCGAT 1 cut(s) 1254
BseDI CCNNGG 3 cut(s) 76, 252, 351
BseGI GGATG 3 cut(s) 196, 401, 819
BseLI CCNNNNNNNGG 3 cut(s) 206, 207, 1401
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 3 cut(s) 1142, 1172, 1268
BseSI GKGCMC 3 cut(s) 313, 690, 1447
BseXI GCAGC 3 cut(s) 484, 580, 922
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 2 cut(s) 350, 1087
BshVI ATCGAT 1 cut(s) 1254
BsiHKAI GWGCWC 2 cut(s) 313, 432
BslFI GGGAC 2 cut(s) 593, 1406
BslI CCNNNNNNNGG 3 cut(s) 206, 207, 1401
BsmAI GTCTC 2 cut(s) 525, 1374
BsmFI GGGAC 2 cut(s) 593, 1406
BsmI GAATGC 3 cut(s) 88, 192, 784
BsnI GGCC 2 cut(s) 350, 1087
Bsp1286I GDGCHC 4 cut(s) 313, 432, 690, 1447
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 3 cut(s) 223, 228, 1045
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 1 cut(s) 1344
BspANI GGCC 2 cut(s) 350, 1087
BspCNI CTCAG 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 1254
BspLI GGNNCC 3 cut(s) 1143, 1266, 1337
BspMAI CTGCAG 1 cut(s) 598
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 2 cut(s) 236, 1040
BsrDI GCAATG 1 cut(s) 426
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 1142, 1172, 1268
BssECI CCNNGG 3 cut(s) 76, 252, 351
BssMI GATC 3 cut(s) 223, 228, 1045
BssT1I CCWWGG 3 cut(s) 76, 252, 351
Bst2UI CCWGG 2 cut(s) 243, 393
Bst4CI ACNGT 4 cut(s) 289, 881, 1012, 1399
Bst6I CTCTTC 4 cut(s) 40, 372, 1003, 1488
BstAPI GCANNNNNTGC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 6 cut(s) 86, 142, 239, 348, 1329, 1437
BstDEI CTNAG 3 cut(s) 105, 613, 1340
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 3 cut(s) 196, 401, 819
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 3 cut(s) 226, 231, 1048
BstMAI GTCTC 2 cut(s) 525, 1374
BstMBI GATC 3 cut(s) 223, 228, 1045
BstMWI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
BstNI CCWGG 2 cut(s) 243, 393
BstSCI CCNGG 2 cut(s) 241, 391
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 3 cut(s) 313, 690, 1447
BstV1I GCAGC 3 cut(s) 484, 580, 922
BstV2I GAAGAC 1 cut(s) 1020
BstXI CCANNNNNNTGG 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 1254
BsuI GTATCC 1 cut(s) 455
BsuRI GGCC 2 cut(s) 350, 1087
BsuTUI ATCGAT 1 cut(s) 1254
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 2 cut(s) 468, 936
BtsCI GGATG 3 cut(s) 196, 401, 819
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 4 cut(s) 789, 1008, 1165, 1404
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 6 cut(s) 86, 142, 239, 348, 1329, 1437
CfoI GCGC 1 cut(s) 100
Cfr13I GGNCC 2 cut(s) 389, 434
ClaI ATCGAT 1 cut(s) 1254
CseI GACGC 1 cut(s) 935
Csp6I GTAC 2 cut(s) 112, 695
CviAII CATG 7 cut(s) 77, 176, 486, 836, 990, 1133, 1146
CviQI GTAC 2 cut(s) 112, 695
DdeI CTNAG 3 cut(s) 105, 613, 1340
DpnI GATC 3 cut(s) 225, 230, 1047
DpnII GATC 3 cut(s) 223, 228, 1045
DriI GACNNNNNGTC 1 cut(s) 1229
Eam1104I CTCTTC 4 cut(s) 40, 372, 1003, 1488
Eam1105I GACNNNNNGTC 1 cut(s) 1229
EarI CTCTTC 4 cut(s) 40, 372, 1003, 1488
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 3 cut(s) 76, 252, 351
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 1252
Eco47I GGWCC 2 cut(s) 389, 434
Eco47III AGCGCT 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 241, 391
EcoRV GATATC 1 cut(s) 1252
EcoT14I CCWWGG 3 cut(s) 76, 252, 351
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 3 cut(s) 76, 252, 351
FaeI CATG 7 cut(s) 80, 179, 489, 839, 993, 1136, 1149
FaqI GGGAC 2 cut(s) 593, 1406
FatI CATG 7 cut(s) 76, 175, 485, 835, 989, 1132, 1145
Fnu4HI GCNGC 3 cut(s) 498, 594, 936
FokI GGATG 3 cut(s) 183, 408, 826
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 3 cut(s) 498, 594, 936
FspBI CTAG 1 cut(s) 1440
GlaI GCGC 1 cut(s) 99
GluI GCNGC 3 cut(s) 498, 594, 936
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 2 cut(s) 350, 1087
HgaI GACGC 1 cut(s) 935
HhaI GCGC 1 cut(s) 100
Hin1II CATG 7 cut(s) 80, 179, 489, 839, 993, 1136, 1149
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 6 cut(s) 169, 356, 518, 1081, 1230, 1303
HphI GGTGA 3 cut(s) 268, 683, 882
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 5 cut(s) 133, 228, 434, 562, 850
Hpy188III TCNNGA 6 cut(s) 638, 1097, 1234, 1292, 1315, 1487
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 6 cut(s) 200, 208, 727, 940, 1254, 1370
HpyCH4III ACNGT 4 cut(s) 289, 881, 1012, 1399
HpyCH4V TGCA 8 cut(s) 140, 311, 500, 596, 683, 707, 782, 860
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
HpyF3I CTNAG 3 cut(s) 105, 613, 1340
Hsp92II CATG 7 cut(s) 80, 179, 489, 839, 993, 1136, 1149
HspAI GCGC 1 cut(s) 98
Kzo9I GATC 3 cut(s) 223, 228, 1045
LmnI GCTCC 2 cut(s) 81, 416
Lsp1109I GCAGC 3 cut(s) 484, 580, 922
LweI GCATC 2 cut(s) 153, 1423
MaeI CTAG 1 cut(s) 1440
MaeIII GTNAC 6 cut(s) 283, 881, 888, 1117, 1270, 1358
MalI GATC 3 cut(s) 225, 230, 1047
MboI GATC 3 cut(s) 223, 228, 1045
MhlI GDGCHC 4 cut(s) 313, 432, 690, 1447
MluCI AATT 6 cut(s) 127, 601, 619, 855, 1040, 1504
MlyI GAGTC 2 cut(s) 527, 1239
MmeI TCCRAC 2 cut(s) 606, 1039
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 3 cut(s) 789, 963, 1507
MslI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
MspA1I CMGCKG 2 cut(s) 109, 593
MspR9I CCNGG 2 cut(s) 243, 393
Mva1269I GAATGC 3 cut(s) 88, 192, 784
MvaI CCWGG 2 cut(s) 243, 393
MwoI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
NcoI CCATGG 1 cut(s) 76
NdeII GATC 3 cut(s) 223, 228, 1045
NlaIII CATG 7 cut(s) 80, 179, 489, 839, 993, 1136, 1149
NlaIV GGNNCC 3 cut(s) 1143, 1266, 1337
NmuCI GTSAC 4 cut(s) 881, 888, 1117, 1270
OliI CACNNNNGTG 1 cut(s) 1367
PctI GAATGC 3 cut(s) 88, 192, 784
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 4 cut(s) 169, 356, 1081, 1303
PkrI GCNGC 3 cut(s) 499, 595, 937
PleI GAGTC 2 cut(s) 526, 1238
PpsI GAGTC 2 cut(s) 526, 1238
PpuMI RGGWCCY 1 cut(s) 389
PshBI ATTAAT 2 cut(s) 789, 963
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 241, 391
PspGI CCWGG 2 cut(s) 241, 391
PspN4I GGNNCC 3 cut(s) 1143, 1266, 1337
PspPI GGNCC 2 cut(s) 389, 434
PspPPI RGGWCCY 1 cut(s) 389
PstI CTGCAG 1 cut(s) 598
PvuII CAGCTG 2 cut(s) 109, 593
RsaI GTAC 2 cut(s) 113, 696
RsaNI GTAC 2 cut(s) 112, 695
RseI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 789, 963, 1507
SatI GCNGC 3 cut(s) 498, 594, 936
Sau3AI GATC 3 cut(s) 223, 228, 1045
Sau96I GGNCC 2 cut(s) 389, 434
SchI GAGTC 2 cut(s) 527, 1239
ScrFI CCNGG 2 cut(s) 243, 393
SduI GDGCHC 4 cut(s) 313, 432, 690, 1447
SfaNI GCATC 2 cut(s) 153, 1423
SfcI CTRYAG 1 cut(s) 594
SinI GGWCC 2 cut(s) 389, 434
SmiMI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
SmlI CTYRAG 4 cut(s) 425, 515, 758, 1292
SmoI CTYRAG 4 cut(s) 425, 515, 758, 1292
Sse9I AATT 6 cut(s) 127, 601, 619, 855, 1040, 1504
SsiI CCGC 1 cut(s) 1344
SspI AATATT 2 cut(s) 7, 772
SspMI CTAG 1 cut(s) 1440
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 241, 391
StyI CCWWGG 3 cut(s) 76, 252, 351
TaaI ACNGT 4 cut(s) 289, 881, 1012, 1399
TaqI TCGA 4 cut(s) 147, 162, 1228, 1254
TaqII GACCGA 1 cut(s) 1334
TasI AATT 6 cut(s) 127, 601, 619, 855, 1040, 1504
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 4 cut(s) 169, 356, 1081, 1303
Tru1I TTAA 3 cut(s) 789, 963, 1507
Tru9I TTAA 3 cut(s) 789, 963, 1507
TscAI CASTG 4 cut(s) 789, 1015, 1172, 1404
TseFI GTSAC 4 cut(s) 881, 888, 1117, 1270
TseI GCWGC 3 cut(s) 497, 593, 935
Tsp45I GTSAC 4 cut(s) 881, 888, 1117, 1270
TspGWI ACGGA 2 cut(s) 431, 1038
TspRI CASTG 4 cut(s) 789, 1015, 1172, 1404
VneI GTGCAC 1 cut(s) 309
VpaK11BI GGWCC 2 cut(s) 389, 434
VspI ATTAAT 2 cut(s) 789, 963
XapI RAATTY 2 cut(s) 127, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 1 cut(s) 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.