pycom02g25050

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010030
Physical Location & Seq
Forward (+)
23079329 .. 23081495
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25050.1

Sequence Viewer

Length: 1305 bp
ATGGCAAAGAAGAGCTGGCTCAGTATAGTAAAGAGGTTCTTTGTTTCAGACACACAATCCAAGCAAAAAAATGAGAAGAGAAGGAGATGGATTTTTGGAAAGCTTAAGATCAAAGGACGACCCTCACTAACAGCACCTTCACCAACATCGCCGTCGAAAGAGAGGACACGAAACGAGTCAGATGAAGAACAGAGCAAGCATGCAATGTCTGTGGCTATTGCCAGCACTGCTGCTGCAGAAGCAGCTGTTGCAGCAGCTCATGCTGCTGTTGAGGTTGTACGGCTCACCGGCACCCAACCATCTTCTTCTGACCAATGTTTGGAGGAGGAGGCAAAAGTGTTGTCAGTCACTACAGCTCAAAGAAAGGCACCTGAAAGGGTACAAGAGTCTGCTGTTGTCAAAATACAAACTGCCTTCAGAGGTTACCTTGCAAGGAAAGCTTTGAGAGCACTGAAGGGTATAGTGAAGCTACAAGCCATTATCCGAGGACGGGCTGTGAGACGCCAAGCAATGACTACGCTCAAGTGCTTGCAGTCCATAATAAACATCCAATCGCATGCCTGCGCAAGGCGACTCCAAACCATTGAAGACGCTTACTATGGTGATGAACACAATGAGTTCGAAAACTTGAGAGACAAGATAATAAGGATGGATTCAAACAGCCAAAGAAGGTGGGATGACAGCGTTACTTCACAGAAAGAGGCAGAGGCCATGTTTCTAAGCAAGAAAGAAGCTATGCTCAAGAGAGAACGGATAAGGGAATACTCGTATAGTCATCGGAAGTCAGCAGAATCAGAGAGAAACAAGGTGAACGGAAGATGGAGATACTGGTTGGACCAATGGGTAGATACACAACTTACTAAAAGTAAAGAGCTTGAGGACTTGGACACAGTTTTTGCTTCAAACGCAAGGCAAAAAGAGGAATTTGGAGAAAAACAACTTAGGATGAGAAACTTTCAGAAACAAAAAATTCAGATCGAAGGAATGGATTCTCCGGTTTTTGTTCCAAGAAGATCTTCGTACCACAAAAAGCAGTGCTCGTTAGGGGATGAGAATTTGTTCGCAAGCTCTCCTGTTGTTCCAACTTACATGGCTGCAACAAAATCTGCCAAGGCAAAAACAAGGTCTCTAAGTTCCCCCAAGGTAAGGGCAGGGAATTTTGATACTTGCTCAGAAAGCTACTCGCCATGCAAGAAGAACATCTCTCTTATATCTTCTGTAGTCAGTGAAATGCCGATAAGCAGTACTACCGCACGAAGAATGGGAAAGTCCAGTAGTCTCCAGCAACGATCGATCCGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

435

Amino Acids

49.24

Weight (kDa)

10.05

Isoelectric Point (pI)

68.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 130 - 149 9.6e-06 IQ calmodulin-binding motif
DUF4005 PF13178 342 - 426 1.4e-10 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 565
AccB1I GGYRCC 2 cut(s) 290, 367
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 1 cut(s) 1251
AclWI GGATC 1 cut(s) 1288
AcsI RAATTY 4 cut(s) 923, 969, 1054, 1156
AcuI CTGAAG 2 cut(s) 400, 473
AcyI GRCGYC 1 cut(s) 502
AfaI GTAC 4 cut(s) 279, 381, 1022, 1246
AfiI CCNNNNNNNGG 4 cut(s) 319, 490, 567, 1146
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 3 cut(s) 587, 657, 903
AjuI GAANNNNNNNTTGG 2 cut(s) 1000, 1032
Alw21I GWGCWC 2 cut(s) 451, 1040
Alw26I GTCTC 4 cut(s) 493, 627, 1131, 1283
AlwI GGATC 1 cut(s) 1288
AoxI GGCC 1 cut(s) 708
ApeKI GCWGC 7 cut(s) 230, 233, 242, 251, 254, 263, 1094
ApoI RAATTY 4 cut(s) 923, 969, 1054, 1156
ArsI GACNNNNNNTTYG 6 cut(s) 326, 358, 878, 910, 1109, 1141
Asp700I GAANNNNTTC 3 cut(s) 988, 1015, 1058
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 1 cut(s) 835
AsuHPI GGTGA 4 cut(s) 132, 277, 614, 820
AsuII TTCGAA 1 cut(s) 621
AvaII GGWCC 1 cut(s) 835
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BanI GGYRCC 2 cut(s) 290, 367
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 2 cut(s) 451, 1040
BbvI GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1081
BccI CCATC 4 cut(s) 81, 307, 643, 813
BceAI ACGGC 2 cut(s) 136, 296
BcoDI GTCTC 4 cut(s) 493, 627, 1131, 1283
BfmI CTRYAG 3 cut(s) 234, 351, 1218
BfrI CTTAAG 1 cut(s) 104
BglII AGATCT 1 cut(s) 1013
BisI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1095
BlsI GCNGC 7 cut(s) 232, 235, 244, 253, 256, 265, 1096
BmcAI AGTACT 1 cut(s) 1246
Bme18I GGWCC 1 cut(s) 835
BmgT120I GGNCC 1 cut(s) 835
BmiI GGNNCC 2 cut(s) 292, 369
BpiI GAAGAC 1 cut(s) 594
BpmI CTGGAG 1 cut(s) 1265
Bpu14I TTCGAA 1 cut(s) 621
BpuEI CTTGAG 4 cut(s) 506, 649, 725, 896
Bsa29I ATCGAT 1 cut(s) 1292
BsaHI GRCGYC 1 cut(s) 502
BsaI GGTCTC 1 cut(s) 1131
BsaJI CCNNGG 3 cut(s) 484, 1110, 1140
BsaWI WCCGGW 1 cut(s) 994
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 490, 567, 1146
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 833, 1272
Bse3DI GCAATG 2 cut(s) 210, 516
BseCI ATCGAT 1 cut(s) 1292
BseDI CCNNGG 3 cut(s) 484, 1110, 1140
BseGI GGATG 5 cut(s) 546, 654, 682, 951, 1054
BseLI CCNNNNNNNGG 4 cut(s) 319, 490, 567, 1146
BseMI GCAATG 2 cut(s) 210, 516
BseMII CTCAG 2 cut(s) 34, 1185
BseNI ACTGG 2 cut(s) 833, 1272
BseRI GAGGAG 2 cut(s) 338, 341
BseXI GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1081
Bsh1285I CGRYCG 1 cut(s) 1292
BshFI GGCC 1 cut(s) 710
BshNI GGYRCC 2 cut(s) 290, 367
BshVI ATCGAT 1 cut(s) 1292
BsiEI CGRYCG 1 cut(s) 1292
BsiHKAI GWGCWC 2 cut(s) 451, 1040
BsiSI CCGG 2 cut(s) 288, 995
BslI CCNNNNNNNGG 4 cut(s) 319, 490, 567, 1146
BsmAI GTCTC 4 cut(s) 493, 627, 1131, 1283
BsmBI CGTCTC 1 cut(s) 493
BsnI GGCC 1 cut(s) 710
Bso31I GGTCTC 1 cut(s) 1131
Bsp119I TTCGAA 1 cut(s) 621
Bsp1286I GDGCHC 2 cut(s) 451, 1040
Bsp143I GATC 5 cut(s) 108, 975, 1013, 1289, 1293
BspACI CCGC 1 cut(s) 1251
BspANI GGCC 1 cut(s) 710
BspCNI CTCAG 2 cut(s) 33, 1184
BspDI ATCGAT 1 cut(s) 1292
BspLI GGNNCC 2 cut(s) 292, 369
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 1 cut(s) 1288
BspQI GCTCTTC 1 cut(s) 5
BspT104I TTCGAA 1 cut(s) 621
BspT107I GGYRCC 2 cut(s) 290, 367
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 1131
BsrDI GCAATG 2 cut(s) 210, 516
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 833, 1272
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 3 cut(s) 484, 1110, 1140
BssMI GATC 5 cut(s) 108, 975, 1013, 1289, 1293
BssNI GRCGYC 1 cut(s) 502
BssT1I CCWWGG 2 cut(s) 1110, 1140
Bst4CI ACNGT 1 cut(s) 892
Bst6I CTCTTC 2 cut(s) 5, 71
BstACI GRCGYC 1 cut(s) 502
BstAFI CTTAAG 1 cut(s) 104
BstAPI GCANNNNNTGC 2 cut(s) 248, 260
BstBI TTCGAA 1 cut(s) 621
BstC8I GCNNGC 8 cut(s) 17, 197, 201, 223, 530, 558, 562, 1066
BstDEI CTNAG 5 cut(s) 20, 719, 941, 1130, 1171
BstEII GGTNACC 1 cut(s) 422
BstENI CCTNNNNNAGG 1 cut(s) 565
BstF5I GGATG 5 cut(s) 546, 654, 682, 951, 1054
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 5 cut(s) 111, 978, 1016, 1292, 1296
BstMAI GTCTC 4 cut(s) 493, 627, 1131, 1283
BstMBI GATC 5 cut(s) 108, 975, 1013, 1289, 1293
BstMCI CGRYCG 1 cut(s) 1292
BstNSI RCATGY 2 cut(s) 203, 560
BstPI GGTNACC 1 cut(s) 422
BstSFI CTRYAG 3 cut(s) 234, 351, 1218
BstV1I GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1081
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1013
Bsu15I ATCGAT 1 cut(s) 1292
BsuRI GGCC 1 cut(s) 710
BsuTUI ATCGAT 1 cut(s) 1292
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 5 cut(s) 546, 654, 682, 951, 1054
BtsI GCAGTG 2 cut(s) 225, 1040
BtsIMutI CAGTG 4 cut(s) 225, 449, 1040, 1231
Cac8I GCNNGC 8 cut(s) 17, 197, 201, 223, 530, 558, 562, 1066
CfoI GCGC 1 cut(s) 566
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 835
ClaI ATCGAT 1 cut(s) 1292
CseI GACGC 2 cut(s) 510, 599
Csp6I GTAC 4 cut(s) 278, 380, 1021, 1245
CspCI CAANNNNNGTGG 4 cut(s) 192, 227, 653, 688
CviAII CATG 6 cut(s) 200, 260, 557, 712, 1090, 1188
CviQI GTAC 4 cut(s) 278, 380, 1021, 1245
DdeI CTNAG 5 cut(s) 20, 719, 941, 1130, 1171
DpnI GATC 5 cut(s) 110, 977, 1015, 1291, 1295
DpnII GATC 5 cut(s) 108, 975, 1013, 1289, 1293
Eam1104I CTCTTC 2 cut(s) 5, 71
EarI CTCTTC 2 cut(s) 5, 71
Eco130I CCWWGG 2 cut(s) 1110, 1140
Eco31I GGTCTC 1 cut(s) 1131
Eco47I GGWCC 1 cut(s) 835
Eco57I CTGAAG 2 cut(s) 400, 473
Eco91I GGTNACC 1 cut(s) 422
EcoNI CCTNNNNNAGG 1 cut(s) 565
EcoO65I GGTNACC 1 cut(s) 422
EcoT14I CCWWGG 2 cut(s) 1110, 1140
ErhI CCWWGG 2 cut(s) 1110, 1140
Esp3I CGTCTC 1 cut(s) 493
FaeI CATG 6 cut(s) 203, 263, 560, 715, 1093, 1191
FalI AAGNNNNNCTT 6 cut(s) 23, 55, 424, 456, 1000, 1032
FatI CATG 6 cut(s) 199, 259, 556, 711, 1089, 1187
Fnu4HI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1095
FokI GGATG 5 cut(s) 533, 661, 689, 958, 1061
Fsp4HI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1095
FspI TGCGCA 1 cut(s) 565
GlaI GCGC 1 cut(s) 565
GluI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1095
GsuI CTGGAG 1 cut(s) 1265
HaeIII GGCC 1 cut(s) 710
HapII CCGG 2 cut(s) 288, 995
HgaI GACGC 2 cut(s) 510, 599
HhaI GCGC 1 cut(s) 566
Hin1I GRCGYC 1 cut(s) 502
Hin1II CATG 6 cut(s) 203, 263, 560, 715, 1093, 1191
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HindIII AAGCTT 2 cut(s) 101, 438
HinfI GANTC 6 cut(s) 176, 386, 573, 653, 791, 989
HpaII CCGG 2 cut(s) 288, 995
HphI GGTGA 4 cut(s) 132, 277, 614, 820
Hpy166II GTNNAC 1 cut(s) 811
Hpy188III TCNNGA 1 cut(s) 742
Hpy8I GTNNAC 1 cut(s) 811
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 6 cut(s) 75, 147, 424, 448, 663, 974
HpyCH4III ACNGT 1 cut(s) 892
HpyCH4V TGCA 7 cut(s) 203, 236, 251, 431, 532, 1097, 1191
HpyF3I CTNAG 5 cut(s) 20, 719, 941, 1130, 1171
Hsp92I GRCGYC 1 cut(s) 502
Hsp92II CATG 6 cut(s) 203, 263, 560, 715, 1093, 1191
HspAI GCGC 1 cut(s) 564
Kzo9I GATC 5 cut(s) 108, 975, 1013, 1289, 1293
LguI GCTCTTC 1 cut(s) 5
Lsp1109I GCAGC 7 cut(s) 217, 220, 250, 254, 263, 266, 1081
MaeIII GTNAC 3 cut(s) 346, 422, 685
MalI GATC 5 cut(s) 110, 977, 1015, 1291, 1295
MboI GATC 5 cut(s) 108, 975, 1013, 1289, 1293
MflI RGATCY 1 cut(s) 1013
MhlI GDGCHC 2 cut(s) 451, 1040
MluCI AATT 4 cut(s) 923, 969, 1054, 1156
MlyI GAGTC 3 cut(s) 185, 395, 567
MmeI TCCRAC 2 cut(s) 813, 1106
MroXI GAANNNNTTC 3 cut(s) 988, 1015, 1058
MseI TTAA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 245
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 2 cut(s) 288, 995
NdeII GATC 5 cut(s) 108, 975, 1013, 1289, 1293
NlaIII CATG 6 cut(s) 203, 263, 560, 715, 1093, 1191
NlaIV GGNNCC 2 cut(s) 292, 369
NmuCI GTSAC 1 cut(s) 346
NsbI TGCGCA 1 cut(s) 565
NspI RCATGY 2 cut(s) 203, 560
NspV TTCGAA 1 cut(s) 621
PaeI GCATGC 2 cut(s) 203, 560
PciSI GCTCTTC 1 cut(s) 5
PcsI WCGNNNNNNNCGW 1 cut(s) 1294
PdmI GAANNNNTTC 3 cut(s) 988, 1015, 1058
PfeI GAWTC 3 cut(s) 653, 791, 989
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 7 cut(s) 232, 235, 244, 253, 256, 265, 1096
Ple19I CGATCG 1 cut(s) 1292
PleI GAGTC 3 cut(s) 184, 394, 567
PpsI GAGTC 3 cut(s) 184, 394, 567
PspEI GGTNACC 1 cut(s) 422
PspN4I GGNNCC 2 cut(s) 292, 369
PspPI GGNCC 1 cut(s) 835
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 1013
PvuI CGATCG 1 cut(s) 1292
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 4 cut(s) 279, 381, 1022, 1246
RsaNI GTAC 4 cut(s) 278, 380, 1021, 1245
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 7 cut(s) 231, 234, 243, 252, 255, 264, 1095
Sau3AI GATC 5 cut(s) 108, 975, 1013, 1289, 1293
Sau96I GGNCC 1 cut(s) 835
ScaI AGTACT 1 cut(s) 1246
SchI GAGTC 3 cut(s) 185, 395, 567
SduI GDGCHC 2 cut(s) 451, 1040
SfcI CTRYAG 3 cut(s) 234, 351, 1218
SfuI TTCGAA 1 cut(s) 621
SinI GGWCC 1 cut(s) 835
SmlI CTYRAG 5 cut(s) 104, 521, 628, 740, 875
SmoI CTYRAG 5 cut(s) 104, 521, 628, 740, 875
SphI GCATGC 2 cut(s) 203, 560
Sse9I AATT 4 cut(s) 923, 969, 1054, 1156
SsiI CCGC 1 cut(s) 1251
StyI CCWWGG 2 cut(s) 1110, 1140
TaaI ACNGT 1 cut(s) 892
TaqI TCGA 4 cut(s) 155, 621, 978, 1292
TasI AATT 4 cut(s) 923, 969, 1054, 1156
TatI WGTACW 1 cut(s) 1244
TfiI GAWTC 3 cut(s) 653, 791, 989
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 4 cut(s) 232, 456, 1040, 1231
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 7 cut(s) 230, 233, 242, 251, 254, 263, 1094
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 2 cut(s) 198, 621
TspGWI ACGGA 2 cut(s) 766, 828
TspRI CASTG 4 cut(s) 232, 456, 1040, 1231
Van91I CCANNNNNTGG 1 cut(s) 319
Vha464I CTTAAG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 835
XagI CCTNNNNNAGG 1 cut(s) 565
XapI RAATTY 4 cut(s) 923, 969, 1054, 1156
XceI RCATGY 2 cut(s) 203, 560
XmnI GAANNNNTTC 3 cut(s) 988, 1015, 1058
ZrmI AGTACT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.