AT5G13890

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005131
Physical Location & Seq
Reverse (-)
4478787 .. 4481149
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13890.2

Sequence Viewer

Length: 933 bp
ATGGGAGAGATGATGTACAAGCTCTTCGCTTCAGCGTCACTACTAACCCTAGGATTGTATCATCTCATCTGCACTTTCTTCCATCTCCTTAAATCTCCCCAATCCTACGCCGCGAAACCCTTTTACCCTTTTCCACGTCTCTCCTCCGTCACCGGAAACAACCACCACAATCACAACCGCCTCCAAAATCTACCACTGTTCATCCTCATACTCTCTCTATTCGTCGCTTTCCTTCACGAAACCCTAATCTCCTTCTACTCCGATCCTCTCGTCAAAGGAAGTACTCCTGTTCACCGATTCTCATCTCTCAATTCCGCCGCCGTGTTCTTCCTCTTCCTCATCATCGCCATTTACTATCTCCTCTCCGATTCAACCTCGTTAATCCCTCTCCCTTCCGATCTCCTCTTCGCACTCGCCTCCGTTGGTTTCTTCCTCCATTACTCCGCCGCGTCTTCATCAGCTGCAATTCAAACATCTGATCTTCAAGCTCATTGTGATTCCCTCTCTGCTCGTATCTCCGCGTTATGCTCTCTCCTCTGTTTGCTTCTCGCTTGCCGTTCAAGACTCTTTATTGCTGACGCTGCTCTCGCTGCTGCCATTTGTCTTCAAGGTCTCTGGCAGCTTCAGACTGGACTCTCTCTCTACGTTGATGGGTTTATTCCTGAGGGATGTCATCGTCTTCTTGATGTTCAAAGCGGTGTTGAAGGATCAACCCAATGTGATATTCAGGACTCTAAGCTAAGAGCTGTTTCTATATTGGATCTAATGTTCACTGTACACGTCGTGCTTGTGGTTATCCTCATCTTTGTGACTTACACGATGGTGGTGATGGCGGCCGGAGTGAGAAGAACTGGTTCTTATGAAGCTTTGCCGACTAACAATGCTGATTCTAATCACATTCAGATGAAGTCGTTGACAGGCACTCAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

310

Amino Acids

33.92

Weight (kDa)

6.85

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 133 - 272 5.2e-34 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 113, 449, 521
AciI CCGC 9 cut(s) 111, 178, 315, 318, 444, 447, 519, 696, 833
AclWI GGATC 3 cut(s) 257, 715, 768
AcoI YGGCCR 1 cut(s) 834
AcuI CTGAAG 2 cut(s) 15, 608
AdeI CACNNNGTG 1 cut(s) 784
AfaI GTAC 3 cut(s) 17, 283, 777
AflIII ACRYGT 1 cut(s) 778
AgsI TTSAA 7 cut(s) 372, 470, 485, 561, 608, 692, 704
AjiI CACGTC 2 cut(s) 137, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 708, 740
AleI CACNNNNGTG 1 cut(s) 821
AloI GAACNNNNNNTCC 2 cut(s) 752, 784
AluBI AGCT 7 cut(s) 22, 461, 488, 622, 739, 746, 866
AluI AGCT 7 cut(s) 22, 461, 488, 622, 739, 746, 866
Alw26I GTCTC 2 cut(s) 143, 617
AlwI GGATC 3 cut(s) 257, 715, 768
AoxI GGCC 1 cut(s) 834
ApeKI GCWGC 5 cut(s) 461, 581, 590, 593, 619
Asp700I GAANNNNTTC 1 cut(s) 853
AspA2I CCTAGG 1 cut(s) 49
AsuHPI GGTGA 3 cut(s) 142, 284, 838
AvrII CCTAGG 1 cut(s) 49
AxyI CCTNAGG 1 cut(s) 663
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
BbsI GAAGAC 3 cut(s) 444, 596, 671
BbvI GCAGC 5 cut(s) 448, 568, 577, 580, 631
BccI CCATC 4 cut(s) 90, 644, 814, 823
BceAI ACGGC 2 cut(s) 305, 540
BcoDI GTCTC 2 cut(s) 143, 617
BfaI CTAG 1 cut(s) 50
BisI GCNGC 9 cut(s) 111, 318, 447, 462, 582, 591, 594, 620, 834
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 9 cut(s) 112, 319, 448, 463, 583, 592, 595, 621, 835
BmcAI AGTACT 1 cut(s) 283
BmgBI CACGTC 2 cut(s) 137, 781
BpiI GAAGAC 3 cut(s) 444, 596, 671
BsaBI GATNNNNATC 2 cut(s) 301, 891
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 634, 856
Bse21I CCTNAGG 1 cut(s) 663
Bse8I GATNNNNATC 2 cut(s) 301, 891
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 2 cut(s) 201, 674
BseJI GATNNNNATC 2 cut(s) 301, 891
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 2 cut(s) 634, 856
BseRI GAGGAG 4 cut(s) 133, 350, 392, 524
BseX3I CGGCCG 1 cut(s) 834
BseXI GCAGC 5 cut(s) 448, 568, 577, 580, 631
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 3 cut(s) 113, 449, 521
Bsh1285I CGRYCG 1 cut(s) 837
BshFI GGCC 1 cut(s) 836
BsiEI CGRYCG 1 cut(s) 837
BsiSI CCGG 2 cut(s) 153, 837
BsmAI GTCTC 2 cut(s) 143, 617
BsmBI CGTCTC 1 cut(s) 143
BsnI GGCC 1 cut(s) 836
Bso31I GGTCTC 1 cut(s) 617
Bsp1407I TGTACA 2 cut(s) 15, 775
Bsp143I GATC 5 cut(s) 262, 397, 478, 707, 760
BspACI CCGC 9 cut(s) 111, 178, 315, 318, 444, 447, 519, 696, 833
BspANI GGCC 1 cut(s) 836
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 3 cut(s) 113, 449, 521
BspPI GGATC 3 cut(s) 257, 715, 768
BspQI GCTCTTC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 617
BsrGI TGTACA 2 cut(s) 15, 775
BsrI ACTGG 2 cut(s) 634, 856
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 5 cut(s) 262, 397, 478, 707, 760
BssT1I CCWWGG 1 cut(s) 49
Bst4CI ACNGT 2 cut(s) 198, 775
Bst6I CTCTTC 3 cut(s) 29, 338, 410
BstAUI TGTACA 2 cut(s) 15, 775
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 4 cut(s) 663, 735, 740, 924
BstF5I GGATG 2 cut(s) 201, 674
BstFNI CGCG 3 cut(s) 113, 449, 521
BstKTI GATC 5 cut(s) 265, 400, 481, 710, 763
BstMAI GTCTC 2 cut(s) 143, 617
BstMBI GATC 5 cut(s) 262, 397, 478, 707, 760
BstMCI CGRYCG 1 cut(s) 837
BstMWI GCNNNNNNNGC 3 cut(s) 581, 587, 590
BstUI CGCG 3 cut(s) 113, 449, 521
BstV1I GCAGC 5 cut(s) 448, 568, 577, 580, 631
BstV2I GAAGAC 3 cut(s) 444, 596, 671
BstX2I RGATCY 1 cut(s) 760
BstYI RGATCY 1 cut(s) 760
BstZI CGGCCG 1 cut(s) 834
Bsu36I CCTNAGG 1 cut(s) 663
BsuRI GGCC 1 cut(s) 836
BtgZI GCGATG 1 cut(s) 328
BtrI CACGTC 2 cut(s) 137, 781
BtsCI GGATG 2 cut(s) 201, 674
BtsIMutI CAGTG 2 cut(s) 194, 771
Cac8I GCNNGC 1 cut(s) 553
CseI GACGC 3 cut(s) 24, 438, 587
Csp6I GTAC 3 cut(s) 16, 282, 776
CviJI RGCY 9 cut(s) 22, 461, 488, 622, 739, 746, 836, 866, 929
CviKI_1 RGCY 9 cut(s) 22, 461, 488, 622, 739, 746, 836, 866, 929
CviQI GTAC 3 cut(s) 16, 282, 776
DdeI CTNAG 4 cut(s) 663, 735, 740, 924
DpnI GATC 5 cut(s) 264, 399, 480, 709, 762
DpnII GATC 5 cut(s) 262, 397, 478, 707, 760
DraIII CACNNNGTG 1 cut(s) 784
EaeI YGGCCR 1 cut(s) 834
EagI CGGCCG 1 cut(s) 834
Eam1104I CTCTTC 3 cut(s) 29, 338, 410
EarI CTCTTC 3 cut(s) 29, 338, 410
EciI GGCGGA 2 cut(s) 304, 433
EclXI CGGCCG 1 cut(s) 834
Eco130I CCWWGG 1 cut(s) 49
Eco31I GGTCTC 1 cut(s) 617
Eco52I CGGCCG 1 cut(s) 834
Eco57I CTGAAG 2 cut(s) 15, 608
Eco81I CCTNAGG 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
Esp3I CGTCTC 1 cut(s) 143
FaiI YATR 4 cut(s) 209, 526, 755, 861
Fnu4HI GCNGC 9 cut(s) 111, 318, 447, 462, 582, 591, 594, 620, 834
FokI GGATG 2 cut(s) 188, 681
Fsp4HI GCNGC 9 cut(s) 111, 318, 447, 462, 582, 591, 594, 620, 834
FspBI CTAG 1 cut(s) 50
GluI GCNGC 9 cut(s) 111, 318, 447, 462, 582, 591, 594, 620, 834
HaeIII GGCC 1 cut(s) 836
HapII CCGG 2 cut(s) 153, 837
HgaI GACGC 3 cut(s) 24, 438, 587
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 7 cut(s) 297, 368, 497, 564, 633, 731, 887
HpaII CCGG 2 cut(s) 153, 837
HphI GGTGA 3 cut(s) 142, 284, 838
Hpy166II GTNNAC 4 cut(s) 292, 771, 778, 915
Hpy188I TCNGA 6 cut(s) 262, 367, 397, 478, 627, 903
Hpy188III TCNNGA 5 cut(s) 236, 561, 662, 683, 728
Hpy8I GTNNAC 4 cut(s) 292, 771, 778, 915
Hpy99I CGWCG 2 cut(s) 227, 785
HpyAV CCTTC 4 cut(s) 242, 262, 402, 698
HpyCH4III ACNGT 2 cut(s) 198, 775
HpyCH4IV ACGT 3 cut(s) 136, 645, 780
HpyCH4V TGCA 2 cut(s) 72, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 581, 587, 590
HpyF3I CTNAG 4 cut(s) 663, 735, 740, 924
HpySE526I ACGT 3 cut(s) 136, 645, 780
Kzo9I GATC 5 cut(s) 262, 397, 478, 707, 760
LguI GCTCTTC 1 cut(s) 29
Lsp1109I GCAGC 5 cut(s) 448, 568, 577, 580, 631
MaeI CTAG 1 cut(s) 50
MaeII ACGT 3 cut(s) 136, 645, 780
MaeIII GTNAC 3 cut(s) 36, 148, 808
MalI GATC 5 cut(s) 264, 399, 480, 709, 762
MboI GATC 5 cut(s) 262, 397, 478, 707, 760
MflI RGATCY 1 cut(s) 760
MluCI AATT 2 cut(s) 310, 465
MlyI GAGTC 3 cut(s) 558, 627, 725
MroXI GAANNNNTTC 1 cut(s) 853
MseI TTAA 2 cut(s) 90, 380
MslI CAYNNNNRTG 3 cut(s) 806, 821, 902
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 2 cut(s) 153, 837
MvnI CGCG 3 cut(s) 113, 449, 521
MwoI GCNNNNNNNGC 3 cut(s) 581, 587, 590
NdeII GATC 5 cut(s) 262, 397, 478, 707, 760
NmuCI GTSAC 3 cut(s) 36, 148, 808
OliI CACNNNNGTG 1 cut(s) 821
PciSI GCTCTTC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 853
PfeI GAWTC 4 cut(s) 297, 368, 497, 887
PkrI GCNGC 9 cut(s) 112, 319, 448, 463, 583, 592, 595, 621, 835
PleI GAGTC 3 cut(s) 558, 627, 725
PpsI GAGTC 3 cut(s) 558, 627, 725
PsuI RGATCY 1 cut(s) 760
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 3 cut(s) 17, 283, 777
RsaNI GTAC 3 cut(s) 16, 282, 776
RseI CAYNNNNRTG 3 cut(s) 806, 821, 902
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 90, 380
SatI GCNGC 9 cut(s) 111, 318, 447, 462, 582, 591, 594, 620, 834
Sau3AI GATC 5 cut(s) 262, 397, 478, 707, 760
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 3 cut(s) 558, 627, 725
SmiMI CAYNNNNRTG 3 cut(s) 806, 821, 902
Sse9I AATT 2 cut(s) 310, 465
SsiI CCGC 9 cut(s) 111, 178, 315, 318, 444, 447, 519, 696, 833
SspMI CTAG 1 cut(s) 50
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 198, 775
TaiI ACGT 3 cut(s) 139, 648, 783
TasI AATT 2 cut(s) 310, 465
TatI WGTACW 3 cut(s) 15, 281, 775
TauI GCSGC 4 cut(s) 113, 320, 449, 836
TfiI GAWTC 4 cut(s) 297, 368, 497, 887
Tru1I TTAA 2 cut(s) 90, 380
Tru9I TTAA 2 cut(s) 90, 380
TscAI CASTG 2 cut(s) 201, 778
TseFI GTSAC 3 cut(s) 36, 148, 808
TseI GCWGC 5 cut(s) 461, 581, 590, 593, 619
Tsp45I GTSAC 3 cut(s) 36, 148, 808
TspDTI ATGAA 4 cut(s) 190, 444, 876, 920
TspGWI ACGGA 2 cut(s) 136, 409
TspRI CASTG 2 cut(s) 201, 778
XmaJI CCTAGG 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 853
XspI CTAG 1 cut(s) 50
ZrmI AGTACT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.