pycom111g00090

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0005131
Physical Location & Seq
Reverse (-)
90414 .. 91361
948 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00090.1

Sequence Viewer

Length: 948 bp
ATGGCAGCCCTAGTGTACCAGATATTGTCTTCCTCCGCCCTGGTATCACTGGGACTCTACCACATGGTGGCCACCACCCGCAACTACCTCAAATCTCCACAGTCCTACGCCGCCAAGCCCTACCACCCCTTCCCCCTCTCCTCTTCCTCTTCCAACCTTAATCCTCCTCATCATCGTCTCAGGTATCTCCAGCTCTATGCCATCATCGCCTGCCTATTGGTCGCCGTCGTCCACCAGACCCTCACCTCCTTCGACGCCGACCCACTCCTCAAGGGCCGAACACCCGTACACCGATTCACGTCCCTGCAGTCCGCGGCCTCCCTCTTCCTCTTTCTGCTGCTCACGCTGGCTGTCCTGCTTTCCGAGTGGGCCCCATCGGTTCTTCCGCTGCCCTCGGATCTGGTGTTCGGGCTGGCCGCGGCCCTCTTCTACCTGCAGTCGCTGGTGTCGTGGGGTGCGGCGTCGGTGCAGATGTCGGATCTGCAGGCTAAGTGCGACTCGGTTTCCGGTCGGATCACGGCGGTGGCGTCGGGGCTGTGCGTGGTTCTGGCGTGCCAGCCGAGGGTGTTCGTAGCGGATGTGGGGTTAGGGGCGGTCATGTGCCTGCAGGGGCTGTGGGTATTGCAGACGGGGCTGTCGCTGTACGTGGAGGCGTTCATCCCGGAGGGGTGCCACAAGCTCCTGGACGTGGTGATCGGGGTGGAGGGTTCCACCAAGTGCGACCTGGACGAGTCCCGGTACAGGGCGGTGGCCACACATATTCTGGATCTGGTGTTCTTGGTGCACGTCATGTTCGTCCTGCTCATTGTCATGGTCGCCTACGCCGTCGTTGCCAAGTTTGTTGGAATCCGAAGATTAGGTTCATATGAGGTATTGCCCAACGCCGCTCCCTCCTCCGACCACAACAACCACATTCAGATGAAAGCCTTGAGCGGCACTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

316

Amino Acids

34.03

Weight (kDa)

8.15

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 133 - 275 5.5e-28 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 634
Acc36I ACCTGC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 669
AccB7I CCANNNNNTGG 1 cut(s) 67
AccBSI CCGCTC 2 cut(s) 887, 933
AccII CGCG 2 cut(s) 314, 419
AclWI GGATC 4 cut(s) 405, 486, 521, 774
AcoI YGGCCR 3 cut(s) 69, 414, 750
AcyI GRCGYC 3 cut(s) 255, 461, 527
AdeI CACNNNGTG 2 cut(s) 67, 717
AfaI GTAC 4 cut(s) 17, 288, 644, 740
AfiI CCNNNNNNNGG 5 cut(s) 67, 562, 688, 741, 742
AjiI CACGTC 3 cut(s) 300, 688, 787
AjnI CCWGG 3 cut(s) 39, 681, 723
AleI CACNNNNGTG 1 cut(s) 521
AloI GAACNNNNNNTCC 4 cut(s) 389, 421, 758, 790
AluBI AGCT 2 cut(s) 193, 679
AluI AGCT 2 cut(s) 193, 679
Alw21I GWGCWC 1 cut(s) 786
Alw26I GTCTC 1 cut(s) 182
Alw44I GTGCAC 1 cut(s) 782
AlwI GGATC 4 cut(s) 405, 486, 521, 774
AlwNI CAGNNNCTG 2 cut(s) 442, 613
AoxI GGCC 7 cut(s) 69, 274, 315, 369, 414, 420, 750
ApaI GGGCCC 1 cut(s) 373
ApaLI GTGCAC 1 cut(s) 782
ApeKI GCWGC 3 cut(s) 5, 337, 388
AspS9I GGNCC 4 cut(s) 274, 369, 370, 421
AsuC2I CCSGG 2 cut(s) 662, 736
AsuHPI GGTGA 2 cut(s) 235, 703
BaeGI GKGCMC 2 cut(s) 373, 786
BalI TGGCCA 2 cut(s) 71, 752
BanI GGYRCC 1 cut(s) 669
BanII GRGCYC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 786
BbvI GCAGC 3 cut(s) 17, 324, 375
BccI CCATC 2 cut(s) 209, 382
BceAI ACGGC 3 cut(s) 209, 534, 809
BciT130I CCWGG 3 cut(s) 41, 683, 725
BcnI CCSGG 2 cut(s) 662, 736
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 4 cut(s) 305, 434, 482, 605
BfuAI ACCTGC 1 cut(s) 441
Bme1390I CCNGG 5 cut(s) 41, 662, 683, 725, 736
BmgBI CACGTC 3 cut(s) 300, 688, 787
BmgT120I GGNCC 4 cut(s) 274, 369, 370, 421
BmiI GGNNCC 4 cut(s) 371, 372, 671, 709
BmrFI CCNGG 5 cut(s) 41, 662, 683, 725, 736
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 21
BplI GAGNNNNNCTC 1 cut(s) 922
BpmI CTGGAG 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 662, 736
BsaAI YACGTR 1 cut(s) 646
BsaHI GRCGYC 3 cut(s) 255, 461, 527
BsaJI CCNNGG 5 cut(s) 39, 312, 393, 417, 560
BsaWI WCCGGW 1 cut(s) 506
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 562, 688, 741, 742
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 3 cut(s) 41, 683, 725
BseDI CCNNGG 5 cut(s) 39, 312, 393, 417, 560
BseGI GGATG 2 cut(s) 583, 657
BseLI CCNNNNNNNGG 5 cut(s) 67, 562, 688, 741, 742
BseMII CTCAG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 4 cut(s) 130, 156, 257, 883
BseSI GKGCMC 2 cut(s) 373, 786
BseXI GCAGC 3 cut(s) 17, 324, 375
BsgI GTGCAG 1 cut(s) 488
Bsh1236I CGCG 2 cut(s) 314, 419
Bsh1285I CGRYCG 1 cut(s) 511
BshFI GGCC 7 cut(s) 71, 276, 317, 371, 416, 422, 752
BshNI GGYRCC 1 cut(s) 669
BsiEI CGRYCG 1 cut(s) 511
BsiHKAI GWGCWC 1 cut(s) 786
BsiSI CCGG 3 cut(s) 507, 662, 736
BslFI GGGAC 3 cut(s) 66, 286, 718
BslI CCNNNNNNNGG 5 cut(s) 67, 562, 688, 741, 742
BsmAI GTCTC 1 cut(s) 182
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 3 cut(s) 66, 286, 718
BsnI GGCC 7 cut(s) 71, 276, 317, 371, 416, 422, 752
Bsp120I GGGCCC 1 cut(s) 369
Bsp1286I GDGCHC 2 cut(s) 373, 786
Bsp143I GATC 5 cut(s) 397, 478, 513, 693, 766
BspANI GGCC 7 cut(s) 71, 276, 317, 371, 416, 422, 752
BspCNI CTCAG 1 cut(s) 192
BspFNI CGCG 2 cut(s) 314, 419
BspLI GGNNCC 4 cut(s) 371, 372, 671, 709
BspMAI CTGCAG 4 cut(s) 309, 438, 486, 609
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 4 cut(s) 405, 486, 521, 774
BspT107I GGYRCC 1 cut(s) 669
BsrBI CCGCTC 2 cut(s) 887, 933
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 5 cut(s) 39, 312, 393, 417, 560
BssMI GATC 5 cut(s) 397, 478, 513, 693, 766
BssNI GRCGYC 3 cut(s) 255, 461, 527
Bst2UI CCWGG 3 cut(s) 41, 683, 725
Bst4CI ACNGT 1 cut(s) 102
Bst6I CTCTTC 4 cut(s) 148, 154, 329, 431
BstACI GRCGYC 3 cut(s) 255, 461, 527
BstBAI YACGTR 1 cut(s) 646
BstC8I GCNNGC 7 cut(s) 211, 348, 414, 486, 553, 557, 605
BstDEI CTNAG 4 cut(s) 179, 489, 939, 945
BstDSI CCRYGG 2 cut(s) 312, 417
BstF5I GGATG 2 cut(s) 583, 657
BstFNI CGCG 2 cut(s) 314, 419
BstKTI GATC 5 cut(s) 400, 481, 516, 696, 769
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 5 cut(s) 397, 478, 513, 693, 766
BstMCI CGRYCG 1 cut(s) 511
BstMWI GCNNNNNNNGC 4 cut(s) 206, 343, 631, 830
BstNI CCWGG 3 cut(s) 41, 683, 725
BstSCI CCNGG 5 cut(s) 39, 660, 681, 723, 734
BstSFI CTRYAG 4 cut(s) 305, 434, 482, 605
BstSLI GKGCMC 2 cut(s) 373, 786
BstUI CGCG 2 cut(s) 314, 419
BstV1I GCAGC 3 cut(s) 17, 324, 375
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 3 cut(s) 397, 478, 766
BstYI RGATCY 3 cut(s) 397, 478, 766
BsuRI GGCC 7 cut(s) 71, 276, 317, 371, 416, 422, 752
BtgI CCRYGG 2 cut(s) 312, 417
BtgZI GCGATG 1 cut(s) 190
BtrI CACGTC 3 cut(s) 300, 688, 787
BtsCI GGATG 2 cut(s) 583, 657
BtsIMutI CAGTG 1 cut(s) 47
BveI ACCTGC 1 cut(s) 441
Cac8I GCNNGC 7 cut(s) 211, 348, 414, 486, 553, 557, 605
CaiI CAGNNNCTG 2 cut(s) 442, 613
Cfr13I GGNCC 4 cut(s) 274, 369, 370, 421
Cfr42I CCGCGG 2 cut(s) 315, 420
CseI GACGC 3 cut(s) 263, 450, 516
Csp6I GTAC 4 cut(s) 16, 287, 643, 739
CviAII CATG 4 cut(s) 64, 598, 790, 811
CviQI GTAC 4 cut(s) 16, 287, 643, 739
DdeI CTNAG 4 cut(s) 179, 489, 939, 945
DpnI GATC 5 cut(s) 399, 480, 515, 695, 768
DpnII GATC 5 cut(s) 397, 478, 513, 693, 766
DraIII CACNNNGTG 2 cut(s) 67, 717
DrdI GACNNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 634
EaeI YGGCCR 3 cut(s) 69, 414, 750
Eam1104I CTCTTC 4 cut(s) 148, 154, 329, 431
EarI CTCTTC 4 cut(s) 148, 154, 329, 431
EciI GGCGGA 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 370
EcoRII CCWGG 3 cut(s) 39, 681, 723
EcoT38I GRGCYC 1 cut(s) 373
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 4 cut(s) 67, 601, 793, 814
FaiI YATR 8 cut(s) 65, 198, 599, 759, 791, 812, 865, 867
FaqI GGGAC 3 cut(s) 66, 286, 718
FatI CATG 4 cut(s) 63, 597, 789, 810
FauI CCCGC 1 cut(s) 86
FauNDI CATATG 1 cut(s) 865
FokI GGATG 2 cut(s) 590, 644
FriOI GRGCYC 1 cut(s) 373
FspBI CTAG 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 173
HaeIII GGCC 7 cut(s) 71, 276, 317, 371, 416, 422, 752
HapII CCGG 3 cut(s) 507, 662, 736
HgaI GACGC 3 cut(s) 263, 450, 516
Hin1I GRCGYC 3 cut(s) 255, 461, 527
Hin1II CATG 4 cut(s) 67, 601, 793, 814
HinfI GANTC 5 cut(s) 54, 294, 497, 731, 846
HpaII CCGG 3 cut(s) 507, 662, 736
HphI GGTGA 2 cut(s) 235, 703
Hpy166II GTNNAC 4 cut(s) 16, 232, 289, 784
Hpy188I TCNGA 7 cut(s) 364, 397, 478, 513, 851, 898, 918
Hpy188III TCNNGA 1 cut(s) 764
Hpy8I GTNNAC 4 cut(s) 16, 232, 289, 784
Hpy99I CGWCG 5 cut(s) 230, 257, 466, 532, 830
HpyAV CCTTC 2 cut(s) 139, 259
HpyCH4III ACNGT 1 cut(s) 102
HpyCH4IV ACGT 4 cut(s) 299, 645, 687, 786
HpyCH4V TGCA 7 cut(s) 307, 436, 469, 484, 607, 625, 784
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 343, 631, 830
HpyF3I CTNAG 4 cut(s) 179, 489, 939, 945
HpySE526I ACGT 4 cut(s) 299, 645, 687, 786
Hsp92I GRCGYC 3 cut(s) 255, 461, 527
Hsp92II CATG 4 cut(s) 67, 601, 793, 814
KspI CCGCGG 2 cut(s) 315, 420
Kzo9I GATC 5 cut(s) 397, 478, 513, 693, 766
LmnI GCTCC 2 cut(s) 684, 892
Lsp1109I GCAGC 3 cut(s) 17, 324, 375
MaeI CTAG 1 cut(s) 11
MaeII ACGT 4 cut(s) 299, 645, 687, 786
MalI GATC 5 cut(s) 399, 480, 515, 695, 768
MbiI CCGCTC 2 cut(s) 887, 933
MboI GATC 5 cut(s) 397, 478, 513, 693, 766
MboII GAAGA 7 cut(s) 21, 135, 141, 316, 374, 418, 864
MflI RGATCY 3 cut(s) 397, 478, 766
MhlI GDGCHC 2 cut(s) 373, 786
MlsI TGGCCA 2 cut(s) 71, 752
MluNI TGGCCA 2 cut(s) 71, 752
MlyI GAGTC 3 cut(s) 48, 491, 740
MmeI TCCRAC 5 cut(s) 177, 456, 491, 823, 921
Mox20I TGGCCA 2 cut(s) 71, 752
MscI TGGCCA 2 cut(s) 71, 752
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 3 cut(s) 521, 809, 917
Msp20I TGGCCA 2 cut(s) 71, 752
MspA1I CMGCKG 3 cut(s) 314, 388, 419
MspI CCGG 3 cut(s) 507, 662, 736
MspR9I CCNGG 5 cut(s) 41, 662, 683, 725, 736
MvaI CCWGG 3 cut(s) 41, 683, 725
MvnI CGCG 2 cut(s) 314, 419
MwoI GCNNNNNNNGC 4 cut(s) 206, 343, 631, 830
NciI CCSGG 2 cut(s) 662, 736
NdeI CATATG 1 cut(s) 865
NdeII GATC 5 cut(s) 397, 478, 513, 693, 766
NlaIII CATG 4 cut(s) 67, 601, 793, 814
NlaIV GGNNCC 4 cut(s) 371, 372, 671, 709
NmeAIII GCCGAG 1 cut(s) 585
OliI CACNNNNGTG 1 cut(s) 521
PcsI WCGNNNNNNNCGW 4 cut(s) 446, 524, 792, 822
PfeI GAWTC 2 cut(s) 294, 846
PflMI CCANNNNNTGG 1 cut(s) 67
PfoI TCCNGGA 2 cut(s) 660, 681
PleI GAGTC 3 cut(s) 48, 491, 739
PpsI GAGTC 3 cut(s) 48, 491, 739
Ppu21I YACGTR 1 cut(s) 646
Psp6I CCWGG 3 cut(s) 39, 681, 723
PspGI CCWGG 3 cut(s) 39, 681, 723
PspN4I GGNNCC 4 cut(s) 371, 372, 671, 709
PspOMI GGGCCC 1 cut(s) 369
PspPI GGNCC 4 cut(s) 274, 369, 370, 421
PstI CTGCAG 4 cut(s) 309, 438, 486, 609
PstNI CAGNNNCTG 2 cut(s) 442, 613
PsuI RGATCY 3 cut(s) 397, 478, 766
RsaI GTAC 4 cut(s) 17, 288, 644, 740
RsaNI GTAC 4 cut(s) 16, 287, 643, 739
RseI CAYNNNNRTG 3 cut(s) 521, 809, 917
SacII CCGCGG 2 cut(s) 315, 420
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 5 cut(s) 397, 478, 513, 693, 766
Sau96I GGNCC 4 cut(s) 274, 369, 370, 421
SbfI CCTGCAGG 1 cut(s) 609
SchI GAGTC 3 cut(s) 48, 491, 740
ScrFI CCNGG 5 cut(s) 41, 662, 683, 725, 736
SdaI CCTGCAGG 1 cut(s) 609
SduI GDGCHC 2 cut(s) 373, 786
SfcI CTRYAG 4 cut(s) 305, 434, 482, 605
Sfr303I CCGCGG 2 cut(s) 315, 420
SgrBI CCGCGG 2 cut(s) 315, 420
SmiMI CAYNNNNRTG 3 cut(s) 521, 809, 917
SmlI CTYRAG 2 cut(s) 269, 928
SmoI CTYRAG 2 cut(s) 269, 928
Sse8387I CCTGCAGG 1 cut(s) 609
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 5 cut(s) 39, 660, 681, 723, 734
TaaI ACNGT 1 cut(s) 102
TaiI ACGT 4 cut(s) 302, 648, 690, 789
TaqI TCGA 1 cut(s) 252
TauI GCSGC 7 cut(s) 113, 317, 419, 422, 461, 887, 936
TfiI GAWTC 2 cut(s) 294, 846
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 54
TseI GCWGC 3 cut(s) 5, 337, 388
TspDTI ATGAA 3 cut(s) 646, 852, 935
TspRI CASTG 1 cut(s) 54
Van91I CCANNNNNTGG 1 cut(s) 67
VneI GTGCAC 1 cut(s) 782
XcmI CCANNNNNNNNNTGG 2 cut(s) 721, 760
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.