MD17G1004200.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005131
Physical Location & Seq
Reverse (-)
354983 .. 355944
962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1004200.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGCAGCCCTAGTATACCAGATTATGTCTTCCTCCGCATTGGTATCACTGGGACTCTACCACATGGTGGCCACCACCCGCAACCACCTCAAATCTCCACAGTCTTATGCCGCCAAGCCCTACCACCCCTTCCCCCTCTCCTCTTCCTCTTCCAACCTTAATCCTCCTCCTCATCATCGCCTCAGGTATCTCCAGCTCTATGCCATAATCGCCTGCCTATTGGTCGCCGTCGTCCACCAGACCCTCACCTCCTTCGACGCCGACCCACTCCTCAAGGGCAGCACCCCCGTCCACCGATTCACGTCCCTGCAGTCCGCGGCCTCCCTCTTCCTCTTTCTGGTGCTCACGCTGGCTCTGCTGCTTTCCGAGTGTGCCCCATCGGTTCTTCCGCTGCCCTCGGATCTGGTGTTCGGGCTGGCCGCGGCCCTCTTCTACCTGCAATCGCTGGTGTCGTGGGGGGCGGCGTCGGTGCAGACGTCGGATCTGCAGGCCAAGTGCGACTCGGTTTCCGGTCGGATCACGGCGGTGGCGTCGGGGCTATGCGCGGTTCTGGCGTGCCAGCCGAGGGTGTTCGTGGCGGATGTGGGGTTAGGGGCGGCCATGTGCTTGCAGGGGCTGTGGGTGTTGCAGACGGGGCTGTCGCTGTACGTGGAGGCGTTCATCCCGGAGGGGTGCCACAGGCTGCTGGACGTGGTAAACGCGGTGGAGGGTTCCACCAAGTGCGACCTGGACGAGTCCCGGTTCAGGGCGGTGGCCATTTTGGATCTGGTGTTCTTGGTGCACGTCATGTTCGTCCTGCTCATTGTCATGGTCACCTACGCCGTCGTTGCCAAGTCTGTTGGAATCCGCAGATTGGGTTCATATGAGGCCTTGCCCAACGCTGCCCCCTCCTCCGACCACAACCACATTCAGATGAAAGCCTTGAGCGGCACTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

314

Amino Acids

33.49

Weight (kDa)

7.65

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 134 - 274 1.8e-31 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 637
AatII GACGTC 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 672
AccB7I CCANNNNNTGG 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 927
AccI GTMKAC 1 cut(s) 15
AccII CGCG 4 cut(s) 317, 422, 545, 701
AclWI GGATC 4 cut(s) 408, 489, 524, 771
AcoI YGGCCR 4 cut(s) 69, 417, 597, 753
AcyI GRCGYC 4 cut(s) 258, 464, 476, 530
AdeI CACNNNGTG 2 cut(s) 67, 720
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 6 cut(s) 67, 337, 565, 744, 745, 853
AjiI CACGTC 3 cut(s) 303, 691, 784
AjnI CCWGG 1 cut(s) 726
AleI CACNNNNGTG 1 cut(s) 524
AloI GAACNNNNNNTCC 4 cut(s) 392, 424, 755, 787
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw21I GWGCWC 2 cut(s) 345, 783
Alw44I GTGCAC 1 cut(s) 779
AlwI GGATC 4 cut(s) 408, 489, 524, 771
AlwNI CAGNNNCTG 1 cut(s) 616
AoxI GGCC 8 cut(s) 69, 318, 417, 423, 489, 597, 753, 867
ApaLI GTGCAC 1 cut(s) 779
ApeKI GCWGC 6 cut(s) 5, 279, 358, 391, 682, 881
AspLEI GCGC 1 cut(s) 545
AspS9I GGNCC 1 cut(s) 424
AsuC2I CCSGG 2 cut(s) 665, 739
AsuHPI GGTGA 2 cut(s) 238, 805
AxyI CCTNAGG 1 cut(s) 182
BaeGI GKGCMC 2 cut(s) 376, 783
BalI TGGCCA 2 cut(s) 71, 755
BanI GGYRCC 1 cut(s) 672
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 345, 783
BbvI GCAGC 6 cut(s) 17, 291, 345, 378, 669, 868
BccI CCATC 1 cut(s) 385
BceAI ACGGC 3 cut(s) 212, 537, 806
BciT130I CCWGG 1 cut(s) 728
BcnI CCSGG 2 cut(s) 665, 739
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 2 cut(s) 308, 485
BfuAI ACCTGC 1 cut(s) 444
Bme1390I CCNGG 3 cut(s) 665, 728, 739
BmgBI CACGTC 3 cut(s) 303, 691, 784
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 674, 712
BmrFI CCNGG 3 cut(s) 665, 728, 739
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 21
BplI GAGNNNNNCTC 1 cut(s) 916
BpmI CTGGAG 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 665, 739
BsaAI YACGTR 1 cut(s) 649
BsaHI GRCGYC 4 cut(s) 258, 464, 476, 530
BsaJI CCNNGG 4 cut(s) 315, 396, 420, 563
BsaWI WCCGGW 1 cut(s) 509
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 337, 565, 744, 745, 853
Bse1I ACTGG 1 cut(s) 54
Bse21I CCTNAGG 1 cut(s) 182
BseBI CCWGG 1 cut(s) 728
BseDI CCNNGG 4 cut(s) 315, 396, 420, 563
BseGI GGATG 2 cut(s) 586, 660
BseLI CCNNNNNNNGG 6 cut(s) 67, 337, 565, 744, 745, 853
BseMII CTCAG 1 cut(s) 196
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 5 cut(s) 130, 156, 159, 260, 880
BseSI GKGCMC 2 cut(s) 376, 783
BseXI GCAGC 6 cut(s) 17, 291, 345, 378, 669, 868
BsgI GTGCAG 1 cut(s) 491
Bsh1236I CGCG 4 cut(s) 317, 422, 545, 701
Bsh1285I CGRYCG 1 cut(s) 514
BshFI GGCC 8 cut(s) 71, 320, 419, 425, 491, 599, 755, 869
BshNI GGYRCC 1 cut(s) 672
BsiEI CGRYCG 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 345, 783
BsiSI CCGG 3 cut(s) 510, 665, 739
BslFI GGGAC 3 cut(s) 66, 289, 721
BslI CCNNNNNNNGG 6 cut(s) 67, 337, 565, 744, 745, 853
BsmFI GGGAC 3 cut(s) 66, 289, 721
BsnI GGCC 8 cut(s) 71, 320, 419, 425, 491, 599, 755, 869
Bsp1286I GDGCHC 3 cut(s) 345, 376, 783
Bsp143I GATC 4 cut(s) 400, 481, 516, 763
BspANI GGCC 8 cut(s) 71, 320, 419, 425, 491, 599, 755, 869
BspCNI CTCAG 1 cut(s) 195
BspFNI CGCG 4 cut(s) 317, 422, 545, 701
BspLI GGNNCC 2 cut(s) 674, 712
BspMAI CTGCAG 2 cut(s) 312, 489
BspMI ACCTGC 1 cut(s) 444
BspPI GGATC 4 cut(s) 408, 489, 524, 771
BspT107I GGYRCC 1 cut(s) 672
BsrBI CCGCTC 1 cut(s) 927
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 4 cut(s) 315, 396, 420, 563
BssMI GATC 4 cut(s) 400, 481, 516, 763
BssNAI GTATAC 1 cut(s) 16
BssNI GRCGYC 4 cut(s) 258, 464, 476, 530
Bst1107I GTATAC 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 728
Bst4CI ACNGT 1 cut(s) 102
Bst6I CTCTTC 4 cut(s) 148, 154, 332, 434
BstACI GRCGYC 4 cut(s) 258, 464, 476, 530
BstBAI YACGTR 1 cut(s) 649
BstC8I GCNNGC 7 cut(s) 214, 351, 417, 489, 556, 560, 608
BstDEI CTNAG 3 cut(s) 182, 933, 939
BstDSI CCRYGG 2 cut(s) 315, 420
BstEII GGTNACC 1 cut(s) 811
BstF5I GGATG 2 cut(s) 586, 660
BstFNI CGCG 4 cut(s) 317, 422, 545, 701
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 4 cut(s) 403, 484, 519, 766
BstMBI GATC 4 cut(s) 400, 481, 516, 763
BstMCI CGRYCG 1 cut(s) 514
BstMWI GCNNNNNNNGC 5 cut(s) 209, 355, 551, 634, 827
BstNI CCWGG 1 cut(s) 728
BstPI GGTNACC 1 cut(s) 811
BstSCI CCNGG 3 cut(s) 663, 726, 737
BstSFI CTRYAG 2 cut(s) 308, 485
BstSLI GKGCMC 2 cut(s) 376, 783
BstUI CGCG 4 cut(s) 317, 422, 545, 701
BstV1I GCAGC 6 cut(s) 17, 291, 345, 378, 669, 868
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 3 cut(s) 400, 481, 763
BstYI RGATCY 3 cut(s) 400, 481, 763
BstZ17I GTATAC 1 cut(s) 16
Bsu36I CCTNAGG 1 cut(s) 182
BsuRI GGCC 8 cut(s) 71, 320, 419, 425, 491, 599, 755, 869
BtgI CCRYGG 2 cut(s) 315, 420
BtgZI GCGATG 1 cut(s) 161
BtrI CACGTC 3 cut(s) 303, 691, 784
BtsCI GGATG 2 cut(s) 586, 660
BtsIMutI CAGTG 1 cut(s) 47
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 7 cut(s) 214, 351, 417, 489, 556, 560, 608
CaiI CAGNNNCTG 1 cut(s) 616
CfoI GCGC 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 424
Cfr42I CCGCGG 2 cut(s) 318, 423
CseI GACGC 3 cut(s) 266, 453, 519
Csp6I GTAC 1 cut(s) 646
CviAII CATG 4 cut(s) 64, 601, 787, 808
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 3 cut(s) 182, 933, 939
DpnI GATC 4 cut(s) 402, 483, 518, 765
DpnII GATC 4 cut(s) 400, 481, 516, 763
DraIII CACNNNGTG 2 cut(s) 67, 720
DrdI GACNNNNNNGTC 1 cut(s) 637
DseDI GACNNNNNNGTC 1 cut(s) 637
EaeI YGGCCR 4 cut(s) 69, 417, 597, 753
Eam1104I CTCTTC 4 cut(s) 148, 154, 332, 434
EarI CTCTTC 4 cut(s) 148, 154, 332, 434
EciI GGCGGA 1 cut(s) 593
Eco147I AGGCCT 1 cut(s) 869
Eco81I CCTNAGG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 811
EcoO65I GGTNACC 1 cut(s) 811
EcoRII CCWGG 1 cut(s) 726
FaeI CATG 4 cut(s) 67, 604, 790, 811
FaqI GGGAC 3 cut(s) 66, 289, 721
FatI CATG 4 cut(s) 63, 600, 786, 807
FauI CCCGC 1 cut(s) 86
FauNDI CATATG 1 cut(s) 862
FblI GTMKAC 1 cut(s) 15
FokI GGATG 2 cut(s) 593, 647
FspBI CTAG 1 cut(s) 11
GlaI GCGC 1 cut(s) 544
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 8 cut(s) 71, 320, 419, 425, 491, 599, 755, 869
HapII CCGG 3 cut(s) 510, 665, 739
HgaI GACGC 3 cut(s) 266, 453, 519
HhaI GCGC 1 cut(s) 545
Hin1I GRCGYC 4 cut(s) 258, 464, 476, 530
Hin1II CATG 4 cut(s) 67, 604, 790, 811
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HinfI GANTC 5 cut(s) 54, 297, 500, 734, 843
HpaII CCGG 3 cut(s) 510, 665, 739
HphI GGTGA 2 cut(s) 238, 805
Hpy166II GTNNAC 5 cut(s) 16, 235, 292, 697, 781
Hpy188I TCNGA 6 cut(s) 367, 400, 481, 516, 895, 912
Hpy8I GTNNAC 5 cut(s) 16, 235, 292, 697, 781
Hpy99I CGWCG 6 cut(s) 233, 260, 469, 481, 535, 827
HpyAV CCTTC 2 cut(s) 139, 262
HpyCH4III ACNGT 1 cut(s) 102
HpyCH4IV ACGT 5 cut(s) 302, 476, 648, 690, 783
HpyCH4V TGCA 7 cut(s) 310, 439, 472, 487, 610, 628, 781
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 355, 551, 634, 827
HpyF3I CTNAG 3 cut(s) 182, 933, 939
HpySE526I ACGT 5 cut(s) 302, 476, 648, 690, 783
Hsp92I GRCGYC 4 cut(s) 258, 464, 476, 530
Hsp92II CATG 4 cut(s) 67, 604, 790, 811
HspAI GCGC 1 cut(s) 543
KspI CCGCGG 2 cut(s) 318, 423
Kzo9I GATC 4 cut(s) 400, 481, 516, 763
Lsp1109I GCAGC 6 cut(s) 17, 291, 345, 378, 669, 868
MaeI CTAG 1 cut(s) 11
MaeII ACGT 5 cut(s) 302, 476, 648, 690, 783
MaeIII GTNAC 1 cut(s) 811
MalI GATC 4 cut(s) 402, 483, 518, 765
MbiI CCGCTC 1 cut(s) 927
MboI GATC 4 cut(s) 400, 481, 516, 763
MboII GAAGA 6 cut(s) 21, 135, 141, 319, 377, 421
MflI RGATCY 3 cut(s) 400, 481, 763
MhlI GDGCHC 3 cut(s) 345, 376, 783
MlsI TGGCCA 2 cut(s) 71, 755
MluNI TGGCCA 2 cut(s) 71, 755
MlyI GAGTC 3 cut(s) 48, 494, 743
MmeI TCCRAC 5 cut(s) 177, 459, 494, 820, 918
Mox20I TGGCCA 2 cut(s) 71, 755
MscI TGGCCA 2 cut(s) 71, 755
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 3 cut(s) 524, 806, 911
Msp20I TGGCCA 2 cut(s) 71, 755
MspA1I CMGCKG 3 cut(s) 317, 391, 422
MspI CCGG 3 cut(s) 510, 665, 739
MspR9I CCNGG 3 cut(s) 665, 728, 739
MvaI CCWGG 1 cut(s) 728
MvnI CGCG 4 cut(s) 317, 422, 545, 701
MwoI GCNNNNNNNGC 5 cut(s) 209, 355, 551, 634, 827
NciI CCSGG 2 cut(s) 665, 739
NdeI CATATG 1 cut(s) 862
NdeII GATC 4 cut(s) 400, 481, 516, 763
NlaIII CATG 4 cut(s) 67, 604, 790, 811
NlaIV GGNNCC 2 cut(s) 674, 712
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 1 cut(s) 811
OliI CACNNNNGTG 1 cut(s) 524
PceI AGGCCT 1 cut(s) 869
PcsI WCGNNNNNNNCGW 4 cut(s) 449, 473, 527, 789
PfeI GAWTC 2 cut(s) 297, 843
PflMI CCANNNNNTGG 1 cut(s) 67
PfoI TCCNGGA 1 cut(s) 663
PleI GAGTC 3 cut(s) 48, 494, 742
PpsI GAGTC 3 cut(s) 48, 494, 742
Ppu21I YACGTR 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 726
PspEI GGTNACC 1 cut(s) 811
PspGI CCWGG 1 cut(s) 726
PspN4I GGNNCC 2 cut(s) 674, 712
PspPI GGNCC 1 cut(s) 424
PstI CTGCAG 2 cut(s) 312, 489
PstNI CAGNNNCTG 1 cut(s) 616
PsuI RGATCY 3 cut(s) 400, 481, 763
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 3 cut(s) 524, 806, 911
SacII CCGCGG 2 cut(s) 318, 423
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 4 cut(s) 400, 481, 516, 763
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 3 cut(s) 48, 494, 743
ScrFI CCNGG 3 cut(s) 665, 728, 739
SduI GDGCHC 3 cut(s) 345, 376, 783
SfcI CTRYAG 2 cut(s) 308, 485
Sfr303I CCGCGG 2 cut(s) 318, 423
SgrBI CCGCGG 2 cut(s) 318, 423
SmiMI CAYNNNNRTG 3 cut(s) 524, 806, 911
SmlI CTYRAG 2 cut(s) 272, 922
SmoI CTYRAG 2 cut(s) 272, 922
SseBI AGGCCT 1 cut(s) 869
SspMI CTAG 1 cut(s) 11
StuI AGGCCT 1 cut(s) 869
StyD4I CCNGG 3 cut(s) 663, 726, 737
TaaI ACNGT 1 cut(s) 102
TaiI ACGT 5 cut(s) 305, 479, 651, 693, 786
TaqI TCGA 1 cut(s) 255
TauI GCSGC 7 cut(s) 113, 320, 422, 425, 464, 599, 930
TfiI GAWTC 2 cut(s) 297, 843
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 6 cut(s) 5, 279, 358, 391, 682, 881
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 3 cut(s) 649, 849, 929
TspRI CASTG 1 cut(s) 54
Van91I CCANNNNNTGG 1 cut(s) 67
VneI GTGCAC 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 2 cut(s) 724, 763
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 11
ZraI GACGTC 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.