MD09G1001100.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005131
Physical Location & Seq
Reverse (-)
180334 .. 183154
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1001100.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGCAGCCCTAGTGTACCAGATATTGTCTTCCTCCGCACTGGTATCACTGGGACTCTACCACATGGTGGCCACCACCCGCAACCACCTCAAATCTCCACAGTCCTACGCCGCCAAGCCCTACCACCCCTTCCCCCTCTCCTCTTCCTCTTCCAACCTTAATCCTCCTCATCATCGTCTCAGGTATCTCCAGCTCTATGCCATCATCGCCTGCCTATTGGTCGCCGTCGTCCACCAGACCCTCACCTCCTTCGACGCCGACCCACTCCTCAAGGGCCGAACACCCGTACACCGATTCACGTCCCTGCAGTCCGCGGCCTCCCTCTTCCTCTTTCTGCTGCTCACGCTGGCTCTCCTGCTTTCCGAGTGGGCCCCATCGGTTCTTCCGCTGCCCTCGGATCTGGTGTTCGGGCTGGCCGCGGCCCTCTTCTACCTGCAGTCGCTGGTGTCGTGGGGTGCGGCGTCGGTGCAGATGTCGGATCTGCAGGCCAAGTGCGACTCGGTTTCCGGTCGGATCACGGCGATGGCGTCGGGGCTGTGCGTGGTTCTGGCGTGCCAGCCGAGGGTGTTCGTAGCGGATGTGGGGTTAGGGGCGGCCATGTGCCTGCAGGGGCTGTGGGTATTGCAGACGGGGCTGTCGCTGTACGTGGAGGCGTTCATCCCGGAGGGGTGCCACAAGCTCTTGGACGTGGTGATCGGGGTGGAGGGTTCCACCAAGTGCGACCTGGACGAGTCCCGGTTCAGGGCGGTGGCCATTCTGGATCTGGTGTTCTTGGTGCACGTCATGTTCGTCCTGCTCATTGTCATGGTCACCTACGCCGTCGTTGCCAAGTTTGTTGGAATCCGAAGATTGGGTTCATATGAGGTATTGCCCAACGCTGCTCCCTCCTCCGACCACAACAACCACATTCAGATGAAAGCCTTGAGCGGCACTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

314

Amino Acids

33.79

Weight (kDa)

8.16

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 133 - 273 2.5e-30 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 634
Acc36I ACCTGC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 669
AccB7I CCANNNNNTGG 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 927
AccII CGCG 2 cut(s) 314, 419
AclWI GGATC 4 cut(s) 405, 486, 521, 768
AcoI YGGCCR 4 cut(s) 69, 414, 594, 750
AcyI GRCGYC 3 cut(s) 255, 461, 527
AdeI CACNNNGTG 2 cut(s) 67, 717
AfaI GTAC 3 cut(s) 17, 288, 644
AfiI CCNNNNNNNGG 5 cut(s) 67, 562, 741, 742, 850
AjiI CACGTC 3 cut(s) 300, 688, 781
AjnI CCWGG 1 cut(s) 723
AloI GAACNNNNNNTCC 4 cut(s) 389, 421, 752, 784
AluBI AGCT 2 cut(s) 193, 679
AluI AGCT 2 cut(s) 193, 679
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 1 cut(s) 182
Alw44I GTGCAC 1 cut(s) 776
AlwI GGATC 4 cut(s) 405, 486, 521, 768
AlwNI CAGNNNCTG 2 cut(s) 442, 613
AoxI GGCC 9 cut(s) 69, 274, 315, 369, 414, 420, 486, 594, 750
ApaI GGGCCC 1 cut(s) 373
ApaLI GTGCAC 1 cut(s) 776
ApeKI GCWGC 4 cut(s) 5, 337, 388, 878
AspS9I GGNCC 4 cut(s) 274, 369, 370, 421
AsuC2I CCSGG 2 cut(s) 662, 736
AsuHPI GGTGA 3 cut(s) 235, 703, 802
BaeGI GKGCMC 2 cut(s) 373, 780
BalI TGGCCA 2 cut(s) 71, 752
BanI GGYRCC 1 cut(s) 669
BanII GRGCYC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 4 cut(s) 17, 324, 375, 865
BccI CCATC 3 cut(s) 209, 382, 517
BceAI ACGGC 3 cut(s) 209, 534, 803
BciT130I CCWGG 1 cut(s) 725
BcnI CCSGG 2 cut(s) 662, 736
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 4 cut(s) 305, 434, 482, 605
BfuAI ACCTGC 1 cut(s) 441
Bme1390I CCNGG 3 cut(s) 662, 725, 736
BmgBI CACGTC 3 cut(s) 300, 688, 781
BmgT120I GGNCC 4 cut(s) 274, 369, 370, 421
BmiI GGNNCC 4 cut(s) 371, 372, 671, 709
BmrFI CCNGG 3 cut(s) 662, 725, 736
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 21
BplI GAGNNNNNCTC 1 cut(s) 916
BpmI CTGGAG 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 662, 736
BsaAI YACGTR 1 cut(s) 646
BsaHI GRCGYC 3 cut(s) 255, 461, 527
BsaJI CCNNGG 4 cut(s) 312, 393, 417, 560
BsaWI WCCGGW 1 cut(s) 506
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 562, 741, 742, 850
Bse1I ACTGG 2 cut(s) 45, 54
BseBI CCWGG 1 cut(s) 725
BseDI CCNNGG 4 cut(s) 312, 393, 417, 560
BseGI GGATG 2 cut(s) 583, 657
BseLI CCNNNNNNNGG 5 cut(s) 67, 562, 741, 742, 850
BseMII CTCAG 1 cut(s) 193
BseNI ACTGG 2 cut(s) 45, 54
BseRI GAGGAG 4 cut(s) 130, 156, 257, 877
BseSI GKGCMC 2 cut(s) 373, 780
BseXI GCAGC 4 cut(s) 17, 324, 375, 865
BsgI GTGCAG 1 cut(s) 488
Bsh1236I CGCG 2 cut(s) 314, 419
Bsh1285I CGRYCG 1 cut(s) 511
BshFI GGCC 9 cut(s) 71, 276, 317, 371, 416, 422, 488, 596, 752
BshNI GGYRCC 1 cut(s) 669
BsiEI CGRYCG 1 cut(s) 511
BsiHKAI GWGCWC 1 cut(s) 780
BsiSI CCGG 3 cut(s) 507, 662, 736
BslFI GGGAC 3 cut(s) 66, 286, 718
BslI CCNNNNNNNGG 5 cut(s) 67, 562, 741, 742, 850
BsmAI GTCTC 1 cut(s) 182
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 3 cut(s) 66, 286, 718
BsnI GGCC 9 cut(s) 71, 276, 317, 371, 416, 422, 488, 596, 752
Bsp120I GGGCCC 1 cut(s) 369
Bsp1286I GDGCHC 2 cut(s) 373, 780
Bsp143I GATC 5 cut(s) 397, 478, 513, 693, 760
BspANI GGCC 9 cut(s) 71, 276, 317, 371, 416, 422, 488, 596, 752
BspCNI CTCAG 1 cut(s) 192
BspFNI CGCG 2 cut(s) 314, 419
BspLI GGNNCC 4 cut(s) 371, 372, 671, 709
BspMAI CTGCAG 4 cut(s) 309, 438, 486, 609
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 4 cut(s) 405, 486, 521, 768
BspT107I GGYRCC 1 cut(s) 669
BsrBI CCGCTC 1 cut(s) 927
BsrI ACTGG 2 cut(s) 45, 54
BssECI CCNNGG 4 cut(s) 312, 393, 417, 560
BssMI GATC 5 cut(s) 397, 478, 513, 693, 760
BssNI GRCGYC 3 cut(s) 255, 461, 527
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 1 cut(s) 102
Bst6I CTCTTC 4 cut(s) 148, 154, 329, 431
BstACI GRCGYC 3 cut(s) 255, 461, 527
BstBAI YACGTR 1 cut(s) 646
BstC8I GCNNGC 7 cut(s) 211, 348, 414, 486, 553, 557, 605
BstDEI CTNAG 3 cut(s) 179, 933, 939
BstDSI CCRYGG 2 cut(s) 312, 417
BstEII GGTNACC 1 cut(s) 808
BstF5I GGATG 2 cut(s) 583, 657
BstFNI CGCG 2 cut(s) 314, 419
BstKTI GATC 5 cut(s) 400, 481, 516, 696, 763
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 5 cut(s) 397, 478, 513, 693, 760
BstMCI CGRYCG 1 cut(s) 511
BstMWI GCNNNNNNNGC 4 cut(s) 206, 343, 631, 824
BstNI CCWGG 1 cut(s) 725
BstPI GGTNACC 1 cut(s) 808
BstSCI CCNGG 3 cut(s) 660, 723, 734
BstSFI CTRYAG 4 cut(s) 305, 434, 482, 605
BstSLI GKGCMC 2 cut(s) 373, 780
BstUI CGCG 2 cut(s) 314, 419
BstV1I GCAGC 4 cut(s) 17, 324, 375, 865
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 3 cut(s) 397, 478, 760
BstYI RGATCY 3 cut(s) 397, 478, 760
BsuRI GGCC 9 cut(s) 71, 276, 317, 371, 416, 422, 488, 596, 752
BtgI CCRYGG 2 cut(s) 312, 417
BtgZI GCGATG 2 cut(s) 190, 536
BtrI CACGTC 3 cut(s) 300, 688, 781
BtsCI GGATG 2 cut(s) 583, 657
BtsIMutI CAGTG 2 cut(s) 38, 47
BveI ACCTGC 1 cut(s) 441
Cac8I GCNNGC 7 cut(s) 211, 348, 414, 486, 553, 557, 605
CaiI CAGNNNCTG 2 cut(s) 442, 613
Cfr13I GGNCC 4 cut(s) 274, 369, 370, 421
Cfr42I CCGCGG 2 cut(s) 315, 420
CseI GACGC 3 cut(s) 263, 450, 516
Csp6I GTAC 3 cut(s) 16, 287, 643
CviAII CATG 4 cut(s) 64, 598, 784, 805
CviQI GTAC 3 cut(s) 16, 287, 643
DdeI CTNAG 3 cut(s) 179, 933, 939
DpnI GATC 5 cut(s) 399, 480, 515, 695, 762
DpnII GATC 5 cut(s) 397, 478, 513, 693, 760
DraIII CACNNNGTG 2 cut(s) 67, 717
DrdI GACNNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 634
EaeI YGGCCR 4 cut(s) 69, 414, 594, 750
Eam1104I CTCTTC 4 cut(s) 148, 154, 329, 431
EarI CTCTTC 4 cut(s) 148, 154, 329, 431
Eco24I GRGCYC 1 cut(s) 373
Eco91I GGTNACC 1 cut(s) 808
EcoO109I RGGNCCY 1 cut(s) 370
EcoO65I GGTNACC 1 cut(s) 808
EcoRII CCWGG 1 cut(s) 723
EcoT38I GRGCYC 1 cut(s) 373
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 4 cut(s) 67, 601, 787, 808
FaiI YATR 7 cut(s) 65, 198, 599, 785, 806, 859, 861
FaqI GGGAC 3 cut(s) 66, 286, 718
FatI CATG 4 cut(s) 63, 597, 783, 804
FauI CCCGC 1 cut(s) 86
FauNDI CATATG 1 cut(s) 859
FokI GGATG 2 cut(s) 590, 644
FriOI GRGCYC 1 cut(s) 373
FspBI CTAG 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 173
HaeIII GGCC 9 cut(s) 71, 276, 317, 371, 416, 422, 488, 596, 752
HapII CCGG 3 cut(s) 507, 662, 736
HgaI GACGC 3 cut(s) 263, 450, 516
Hin1I GRCGYC 3 cut(s) 255, 461, 527
Hin1II CATG 4 cut(s) 67, 601, 787, 808
HinfI GANTC 5 cut(s) 54, 294, 497, 731, 840
HpaII CCGG 3 cut(s) 507, 662, 736
HphI GGTGA 3 cut(s) 235, 703, 802
Hpy166II GTNNAC 4 cut(s) 16, 232, 289, 778
Hpy188I TCNGA 7 cut(s) 364, 397, 478, 513, 845, 892, 912
Hpy188III TCNNGA 1 cut(s) 758
Hpy8I GTNNAC 4 cut(s) 16, 232, 289, 778
Hpy99I CGWCG 5 cut(s) 230, 257, 466, 532, 824
HpyAV CCTTC 2 cut(s) 139, 259
HpyCH4III ACNGT 1 cut(s) 102
HpyCH4IV ACGT 4 cut(s) 299, 645, 687, 780
HpyCH4V TGCA 7 cut(s) 307, 436, 469, 484, 607, 625, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 343, 631, 824
HpyF3I CTNAG 3 cut(s) 179, 933, 939
HpySE526I ACGT 4 cut(s) 299, 645, 687, 780
Hsp92I GRCGYC 3 cut(s) 255, 461, 527
Hsp92II CATG 4 cut(s) 67, 601, 787, 808
KspI CCGCGG 2 cut(s) 315, 420
Kzo9I GATC 5 cut(s) 397, 478, 513, 693, 760
LmnI GCTCC 1 cut(s) 886
Lsp1109I GCAGC 4 cut(s) 17, 324, 375, 865
MaeI CTAG 1 cut(s) 11
MaeII ACGT 4 cut(s) 299, 645, 687, 780
MaeIII GTNAC 1 cut(s) 808
MalI GATC 5 cut(s) 399, 480, 515, 695, 762
MbiI CCGCTC 1 cut(s) 927
MboI GATC 5 cut(s) 397, 478, 513, 693, 760
MboII GAAGA 7 cut(s) 21, 135, 141, 316, 374, 418, 858
MflI RGATCY 3 cut(s) 397, 478, 760
MhlI GDGCHC 2 cut(s) 373, 780
MlsI TGGCCA 2 cut(s) 71, 752
MluNI TGGCCA 2 cut(s) 71, 752
MlyI GAGTC 3 cut(s) 48, 491, 740
MmeI TCCRAC 5 cut(s) 177, 456, 491, 817, 915
Mox20I TGGCCA 2 cut(s) 71, 752
MscI TGGCCA 2 cut(s) 71, 752
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 3 cut(s) 521, 803, 911
Msp20I TGGCCA 2 cut(s) 71, 752
MspA1I CMGCKG 3 cut(s) 314, 388, 419
MspI CCGG 3 cut(s) 507, 662, 736
MspR9I CCNGG 3 cut(s) 662, 725, 736
MvaI CCWGG 1 cut(s) 725
MvnI CGCG 2 cut(s) 314, 419
MwoI GCNNNNNNNGC 4 cut(s) 206, 343, 631, 824
NciI CCSGG 2 cut(s) 662, 736
NdeI CATATG 1 cut(s) 859
NdeII GATC 5 cut(s) 397, 478, 513, 693, 760
NlaIII CATG 4 cut(s) 67, 601, 787, 808
NlaIV GGNNCC 4 cut(s) 371, 372, 671, 709
NmeAIII GCCGAG 1 cut(s) 585
NmuCI GTSAC 1 cut(s) 808
PcsI WCGNNNNNNNCGW 3 cut(s) 446, 524, 786
PfeI GAWTC 2 cut(s) 294, 840
PflMI CCANNNNNTGG 1 cut(s) 67
PfoI TCCNGGA 1 cut(s) 660
PleI GAGTC 3 cut(s) 48, 491, 739
PpsI GAGTC 3 cut(s) 48, 491, 739
Ppu21I YACGTR 1 cut(s) 646
Psp6I CCWGG 1 cut(s) 723
PspEI GGTNACC 1 cut(s) 808
PspGI CCWGG 1 cut(s) 723
PspN4I GGNNCC 4 cut(s) 371, 372, 671, 709
PspOMI GGGCCC 1 cut(s) 369
PspPI GGNCC 4 cut(s) 274, 369, 370, 421
PstI CTGCAG 4 cut(s) 309, 438, 486, 609
PstNI CAGNNNCTG 2 cut(s) 442, 613
PsuI RGATCY 3 cut(s) 397, 478, 760
RsaI GTAC 3 cut(s) 17, 288, 644
RsaNI GTAC 3 cut(s) 16, 287, 643
RseI CAYNNNNRTG 3 cut(s) 521, 803, 911
SacII CCGCGG 2 cut(s) 315, 420
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 5 cut(s) 397, 478, 513, 693, 760
Sau96I GGNCC 4 cut(s) 274, 369, 370, 421
SbfI CCTGCAGG 1 cut(s) 609
SchI GAGTC 3 cut(s) 48, 491, 740
ScrFI CCNGG 3 cut(s) 662, 725, 736
SdaI CCTGCAGG 1 cut(s) 609
SduI GDGCHC 2 cut(s) 373, 780
SfcI CTRYAG 4 cut(s) 305, 434, 482, 605
Sfr303I CCGCGG 2 cut(s) 315, 420
SgrBI CCGCGG 2 cut(s) 315, 420
SmiMI CAYNNNNRTG 3 cut(s) 521, 803, 911
SmlI CTYRAG 2 cut(s) 269, 922
SmoI CTYRAG 2 cut(s) 269, 922
Sse8387I CCTGCAGG 1 cut(s) 609
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 3 cut(s) 660, 723, 734
TaaI ACNGT 1 cut(s) 102
TaiI ACGT 4 cut(s) 302, 648, 690, 783
TaqI TCGA 1 cut(s) 252
TauI GCSGC 7 cut(s) 113, 317, 419, 422, 461, 596, 930
TfiI GAWTC 2 cut(s) 294, 840
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 2 cut(s) 45, 54
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 4 cut(s) 5, 337, 388, 878
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 3 cut(s) 646, 846, 929
TspRI CASTG 2 cut(s) 45, 54
Van91I CCANNNNNTGG 1 cut(s) 67
VneI GTGCAC 1 cut(s) 776
XcmI CCANNNNNNNNNTGG 2 cut(s) 721, 760
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.