RLG00000019674

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005131
Physical Location & Seq
Forward (+)
55308692 .. 55310727
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019674

Sequence Viewer

Length: 885 bp
ATGGCAACCCTAGTGTACCAGATGATCTCGTCCACAGCACTGGTCTCAATGGGACTCTACCACATGATCGCCACTACCCGCAACCACCTGAGATTCCCACAGTCCTATTTCGCTAGACCCTACCACCCTTTCCCTCTCTCCACCTCCTCATCATCCCACAATCACCGCCTAAAGTTCCTCCAGCTCTACCTCGTCATTGCATTCCTCGCCGTCGCCATAGTCCACCAAACAGTCGCCTCCTTAGACGCCGATCCACTCCTCAAGGGTCGCACGCCTGTCCACTACTTCACCTCCCTCCAGTCCGCCGCCTCCCTCTTCCTCTTTCTACTCCTCACACTGGCGCTCCTTCTCTCCGAGTGGATCCCATCTTTGCTGCCCCTCCCAACAGACCTCATCTTCGGCCTCGCCGCCGCCCTCTTCTACTTGCAGTCATTCGTCTCTTGGGGCGCCGCAGCCGTCCAGACATCCGACCTTCAGGCCAAATGTGACTCCGTCTCAGGAAGGATATCCGCTCAAGCCTCCATCCTCTGCCTCGTCTTAGCTTGCCAGCCAAGACTATTCGTGGCAGACGTAGGCTTGGGCGCTTCCGTGTCTCTGCAGGGCCTGTGGGTCCTGCAGACGGGCCTCTCCCTCTACGTGGATGCCTTCATCCCAGAGGGCTGCCACAAGCTTCTTGATGTGGTCAATGGAGTCGAGGGTTCAACCAAGTGTGATTTGGACGAGTCCAAGTTCAGGGCTGTGGCCATTCTGGATCTAGTGTTCTTGGTGCACGTAATGTTCGTGTTGCTGATTGTGATGGTCACCTATGCAGTGACTGCCAAGTCGGGCAGTCCACAGCTTTATCATCCGGATCGTGCTATAGTGGCTGCTTCATCGCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

295

Amino Acids

31.93

Weight (kDa)

7.14

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 130 - 270 4.4e-32 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 820
AccB1I GGYRCC 1 cut(s) 446
AccBSI CCGCTC 1 cut(s) 512
AccIII TCCGGA 1 cut(s) 847
AciI CCGC 8 cut(s) 79, 166, 303, 306, 408, 411, 450, 510
AclWI GGATC 5 cut(s) 245, 355, 368, 759, 858
AcoI YGGCCR 1 cut(s) 741
AcuI CTGAAG 1 cut(s) 458
AcyI GRCGYC 2 cut(s) 246, 447
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
AgsI TTSAA 1 cut(s) 702
AloI GAACNNNNNNTCC 2 cut(s) 743, 775
AluBI AGCT 4 cut(s) 184, 542, 670, 838
AluI AGCT 4 cut(s) 184, 542, 670, 838
Alw21I GWGCWC 1 cut(s) 771
Alw26I GTCTC 4 cut(s) 49, 442, 499, 597
Alw44I GTGCAC 1 cut(s) 767
AlwI GGATC 5 cut(s) 245, 355, 368, 759, 858
AlwNI CAGNNNCTG 2 cut(s) 604, 815
Aor13HI TCCGGA 1 cut(s) 847
AoxI GGCC 5 cut(s) 400, 477, 601, 622, 741
ApaLI GTGCAC 1 cut(s) 767
ApeKI GCWGC 4 cut(s) 373, 452, 660, 866
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
AspLEI GCGC 3 cut(s) 343, 449, 584
AspS9I GGNCC 3 cut(s) 601, 610, 622
AsuHPI GGTGA 3 cut(s) 155, 280, 793
AvaII GGWCC 1 cut(s) 610
BaeGI GKGCMC 1 cut(s) 771
BalI TGGCCA 1 cut(s) 743
BamHI GGATCC 1 cut(s) 360
BanI GGYRCC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 771
BbvI GCAGC 4 cut(s) 360, 464, 647, 853
BccI CCATC 3 cut(s) 373, 530, 790
BceAI ACGGC 2 cut(s) 194, 440
BcoDI GTCTC 4 cut(s) 49, 442, 499, 597
BfaI CTAG 3 cut(s) 11, 114, 755
BfmI CTRYAG 3 cut(s) 596, 614, 858
BfoI RGCGCY 3 cut(s) 344, 450, 585
BisI GCNGC 8 cut(s) 306, 374, 408, 411, 450, 453, 661, 867
BlsI GCNGC 8 cut(s) 307, 375, 409, 412, 451, 454, 662, 868
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 3 cut(s) 601, 610, 622
BmiI GGNNCC 3 cut(s) 362, 448, 611
BmsI GCATC 1 cut(s) 631
BpmI CTGGAG 2 cut(s) 164, 281
BpuEI CTTGAG 2 cut(s) 245, 498
BsaAI YACGTR 2 cut(s) 637, 772
BsaHI GRCGYC 2 cut(s) 246, 447
BsaI GGTCTC 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 327, 357
Bsc4I CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
Bse1I ACTGG 3 cut(s) 45, 298, 342
Bse3DI GCAATG 1 cut(s) 195
BseAI TCCGGA 1 cut(s) 847
BseGI GGATG 6 cut(s) 152, 464, 522, 646, 648, 844
BseLI CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 2 cut(s) 80, 510
BseNI ACTGG 3 cut(s) 45, 298, 342
BseRI GAGGAG 3 cut(s) 136, 248, 320
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 4 cut(s) 360, 464, 647, 853
BshFI GGCC 5 cut(s) 402, 479, 603, 624, 743
BshNI GGYRCC 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 771
BsiSI CCGG 1 cut(s) 848
BslFI GGGAC 1 cut(s) 66
BslI CCNNNNNNNGG 4 cut(s) 337, 619, 637, 732
BsmAI GTCTC 4 cut(s) 49, 442, 499, 597
BsmBI CGTCTC 2 cut(s) 442, 499
BsmFI GGGAC 1 cut(s) 66
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 5 cut(s) 402, 479, 603, 624, 743
Bso31I GGTCTC 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 771
Bsp13I TCCGGA 1 cut(s) 847
Bsp143I GATC 6 cut(s) 24, 66, 250, 360, 751, 850
BspACI CCGC 8 cut(s) 79, 166, 303, 306, 408, 411, 450, 510
BspANI GGCC 5 cut(s) 402, 479, 603, 624, 743
BspCNI CTCAG 2 cut(s) 81, 509
BspEI TCCGGA 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 362, 448, 611
BspMAI CTGCAG 2 cut(s) 600, 618
BspPI GGATC 5 cut(s) 245, 355, 368, 759, 858
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 49
BsrBI CCGCTC 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 3 cut(s) 45, 298, 342
BssMI GATC 6 cut(s) 24, 66, 250, 360, 751, 850
BssNI GRCGYC 2 cut(s) 246, 447
Bst4CI ACNGT 2 cut(s) 102, 232
Bst6I CTCTTC 2 cut(s) 320, 422
BstACI GRCGYC 2 cut(s) 246, 447
BstAPI GCANNNNNTGC 1 cut(s) 815
BstBAI YACGTR 2 cut(s) 637, 772
BstC8I GCNNGC 3 cut(s) 272, 544, 548
BstDEI CTNAG 4 cut(s) 89, 241, 496, 538
BstEII GGTNACC 1 cut(s) 799
BstF5I GGATG 6 cut(s) 152, 464, 522, 646, 648, 844
BstH2I RGCGCY 3 cut(s) 344, 450, 585
BstHHI GCGC 3 cut(s) 343, 449, 584
BstKTI GATC 6 cut(s) 27, 69, 253, 363, 754, 853
BstMAI GTCTC 4 cut(s) 49, 442, 499, 597
BstMBI GATC 6 cut(s) 24, 66, 250, 360, 751, 850
BstMWI GCNNNNNNNGC 3 cut(s) 206, 815, 863
BstPI GGTNACC 1 cut(s) 799
BstSFI CTRYAG 3 cut(s) 596, 614, 858
BstSLI GKGCMC 1 cut(s) 771
BstV1I GCAGC 4 cut(s) 360, 464, 647, 853
BstX2I RGATCY 2 cut(s) 360, 751
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 2 cut(s) 360, 751
BsuRI GGCC 5 cut(s) 402, 479, 603, 624, 743
BtgZI GCGATG 1 cut(s) 858
BtsCI GGATG 6 cut(s) 152, 464, 522, 646, 648, 844
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 3 cut(s) 38, 335, 816
Cac8I GCNNGC 3 cut(s) 272, 544, 548
CaiI CAGNNNCTG 2 cut(s) 604, 815
CfoI GCGC 3 cut(s) 343, 449, 584
Cfr13I GGNCC 3 cut(s) 601, 610, 622
CseI GACGC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 16
CviAII CATG 1 cut(s) 64
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 4 cut(s) 89, 241, 496, 538
DinI GGCGCC 1 cut(s) 448
DpnI GATC 6 cut(s) 26, 68, 252, 362, 753, 852
DpnII GATC 6 cut(s) 24, 66, 250, 360, 751, 850
DrdI GACNNNNNNGTC 1 cut(s) 820
DseDI GACNNNNNNGTC 1 cut(s) 820
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 2 cut(s) 320, 422
EarI CTCTTC 2 cut(s) 320, 422
EciI GGCGGA 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 507
Eco47I GGWCC 1 cut(s) 610
Eco57I CTGAAG 1 cut(s) 458
Eco91I GGTNACC 1 cut(s) 799
EcoO109I RGGNCCY 2 cut(s) 601, 610
EcoO65I GGTNACC 1 cut(s) 799
EcoRV GATATC 1 cut(s) 507
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
Esp3I CGTCTC 2 cut(s) 442, 499
FaeI CATG 1 cut(s) 67
FaiI YATR 5 cut(s) 65, 218, 807, 860, 883
FaqI GGGAC 1 cut(s) 66
FatI CATG 1 cut(s) 63
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 8 cut(s) 306, 374, 408, 411, 450, 453, 661, 867
FokI GGATG 6 cut(s) 139, 451, 509, 635, 653, 831
Fsp4HI GCNGC 8 cut(s) 306, 374, 408, 411, 450, 453, 661, 867
FspBI CTAG 3 cut(s) 11, 114, 755
GlaI GCGC 3 cut(s) 342, 448, 583
GluI GCNGC 8 cut(s) 306, 374, 408, 411, 450, 453, 661, 867
GsuI CTGGAG 2 cut(s) 164, 281
HaeII RGCGCY 3 cut(s) 344, 450, 585
HaeIII GGCC 5 cut(s) 402, 479, 603, 624, 743
HapII CCGG 1 cut(s) 848
HgaI GACGC 1 cut(s) 254
HhaI GCGC 3 cut(s) 343, 449, 584
Hin1I GRCGYC 2 cut(s) 246, 447
Hin1II CATG 1 cut(s) 67
Hin6I GCGC 3 cut(s) 341, 447, 582
HinP1I GCGC 3 cut(s) 341, 447, 582
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 5 cut(s) 54, 93, 488, 690, 722
HpaII CCGG 1 cut(s) 848
HphI GGTGA 3 cut(s) 155, 280, 793
Hpy166II GTNNAC 6 cut(s) 16, 33, 223, 280, 769, 833
Hpy188I TCNGA 2 cut(s) 355, 469
Hpy188III TCNNGA 5 cut(s) 460, 498, 674, 749, 848
Hpy8I GTNNAC 6 cut(s) 16, 33, 223, 280, 769, 833
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 356, 482, 495, 655
HpyCH4III ACNGT 2 cut(s) 102, 232
HpyCH4IV ACGT 3 cut(s) 570, 636, 771
HpyCH4V TGCA 6 cut(s) 200, 427, 598, 616, 769, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 815, 863
HpyF3I CTNAG 4 cut(s) 89, 241, 496, 538
HpySE526I ACGT 3 cut(s) 570, 636, 771
Hsp92I GRCGYC 2 cut(s) 246, 447
Hsp92II CATG 1 cut(s) 67
HspAI GCGC 3 cut(s) 341, 447, 582
KasI GGCGCC 1 cut(s) 446
Kpn2I TCCGGA 1 cut(s) 847
Kzo9I GATC 6 cut(s) 24, 66, 250, 360, 751, 850
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 4 cut(s) 360, 464, 647, 853
LweI GCATC 1 cut(s) 631
MaeI CTAG 3 cut(s) 11, 114, 755
MaeII ACGT 3 cut(s) 570, 636, 771
MaeIII GTNAC 3 cut(s) 485, 799, 811
MalI GATC 6 cut(s) 26, 68, 252, 362, 753, 852
MbiI CCGCTC 1 cut(s) 512
MboI GATC 6 cut(s) 24, 66, 250, 360, 751, 850
MboII GAAGA 3 cut(s) 307, 388, 409
MflI RGATCY 2 cut(s) 360, 751
MhlI GDGCHC 1 cut(s) 771
MlsI TGGCCA 1 cut(s) 743
MluNI TGGCCA 1 cut(s) 743
Mly113I GGCGCC 1 cut(s) 447
MlyI GAGTC 4 cut(s) 48, 482, 699, 731
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 1 cut(s) 743
MroI TCCGGA 1 cut(s) 847
MscI TGGCCA 1 cut(s) 743
Msp20I TGGCCA 1 cut(s) 743
MspI CCGG 1 cut(s) 848
Mva1269I GAATGC 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 206, 815, 863
NarI GGCGCC 1 cut(s) 447
NdeII GATC 6 cut(s) 24, 66, 250, 360, 751, 850
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 3 cut(s) 362, 448, 611
NmuCI GTSAC 3 cut(s) 485, 799, 811
PcsI WCGNNNNNNNCGW 2 cut(s) 567, 777
PctI GAATGC 1 cut(s) 200
PfeI GAWTC 1 cut(s) 93
PflFI GACNNNGTC 1 cut(s) 491
PkrI GCNGC 8 cut(s) 307, 375, 409, 412, 451, 454, 662, 868
PleI GAGTC 4 cut(s) 48, 482, 698, 730
PluTI GGCGCC 1 cut(s) 450
PpsI GAGTC 4 cut(s) 48, 482, 698, 730
Ppu21I YACGTR 2 cut(s) 637, 772
PpuMI RGGWCCY 1 cut(s) 610
Psp5II RGGWCCY 1 cut(s) 610
PspEI GGTNACC 1 cut(s) 799
PspN4I GGNNCC 3 cut(s) 362, 448, 611
PspPI GGNCC 3 cut(s) 601, 610, 622
PspPPI RGGWCCY 1 cut(s) 610
PstI CTGCAG 2 cut(s) 600, 618
PstNI CAGNNNCTG 2 cut(s) 604, 815
PsuI RGATCY 2 cut(s) 360, 751
PsyI GACNNNGTC 1 cut(s) 491
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SatI GCNGC 8 cut(s) 306, 374, 408, 411, 450, 453, 661, 867
Sau3AI GATC 6 cut(s) 24, 66, 250, 360, 751, 850
Sau96I GGNCC 3 cut(s) 601, 610, 622
SchI GAGTC 4 cut(s) 48, 482, 699, 731
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 3 cut(s) 596, 614, 858
SfoI GGCGCC 1 cut(s) 448
SinI GGWCC 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 260, 513
SmoI CTYRAG 2 cut(s) 260, 513
SsiI CCGC 8 cut(s) 79, 166, 303, 306, 408, 411, 450, 510
SspDI GGCGCC 1 cut(s) 446
SspMI CTAG 3 cut(s) 11, 114, 755
TaaI ACNGT 2 cut(s) 102, 232
TaiI ACGT 3 cut(s) 573, 639, 774
TaqI TCGA 1 cut(s) 693
TauI GCSGC 4 cut(s) 308, 410, 413, 452
TfiI GAWTC 1 cut(s) 93
TscAI CASTG 3 cut(s) 45, 342, 816
TseFI GTSAC 3 cut(s) 485, 799, 811
TseI GCWGC 4 cut(s) 373, 452, 660, 866
Tsp45I GTSAC 3 cut(s) 485, 799, 811
TspDTI ATGAA 2 cut(s) 637, 861
TspGWI ACGGA 2 cut(s) 481, 577
TspRI CASTG 3 cut(s) 45, 342, 816
Tth111I GACNNNGTC 1 cut(s) 491
VneI GTGCAC 1 cut(s) 767
VpaK11BI GGWCC 1 cut(s) 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XspI CTAG 3 cut(s) 11, 114, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.