FvH4_6g31080

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005131
Physical Location & Seq
Forward (+)
24250855 .. 24254244
3390 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31080.t1

Sequence Viewer

Length: 918 bp
ATGGCAGCCCTAGTGTACCAGATGATATCCTCTGCAGCACTGGTCTCACTGGGACTCTACCACATGGTTGCCACCACACGCAACCACCTGAGATCACCGCAGGCCTATTTTGCCAGACCTTATCACCCTTTCCCTCTGTCCTCATCCTCATCGCCGTCCAACAACCACCGTCTCAGGTTCCTCCAGCTCTACCTTGTCATTGCATTCCTCGTTGTCGCTATAGTCCACCAGATAGTCATTTCCTTAGACGCTGATCCGCTCCTCAAGGGTCGCTCACCGGTCCACTACTTTACCTCCCTCCAGTCCGCTGCCTCCCTCTTCCTCTTTCTACTCCTCACACTGGCACTCCTCCTCTCCGAGTGGATCCCAGCTTTGCTGCCCCTCCCCAACGACCTCATCTTCGGCCTCGCTGCAGGCCTCTTCTACTTGCAGTCATTTGTCTTTTGGGGTGCTGCCACTGTCCAGACATCCGACCTTCAGGCCAAATGTGACTCTGTCTCGGGACGGATATCCGCTCTAGCCTCCATCCTCTGCCTCGTCTTGGCTTGCCAGCCAAAACTATTCGTGGCAGACGTAGGCTTGGGCGCTTCCCTGTCTCTACAGGGCCTATGGGTCCTACAGACGGGCCTCTCCCTCTATGTGGATGCCTTCATTCCGGAGGGCTGCCACAAGCTTCTTGATGTGGTAAATGGAGTCGAGGGTTCAACCAAGTGTGACTTAGACGAGTCCAAGTTAAGGGCTGTGGCCATTCTGGATCTGGTGTTCTTGGTGCACGTAATGTTTGTGTTGCTTATTGTGATGGTCACCTATGCAATGACTGCCAAGTCGGTTGGTATTCGAGCATTGGGTTCTTACGAGGCCTTGCCCAGTGACAACACCCACATTCAGATGAAAGCCTTGAGTGGCACCCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

306

Amino Acids

33.02

Weight (kDa)

7.11

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 131 - 271 2.6e-33 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 823
AccB1I GGYRCC 1 cut(s) 905
AccBSI CCGCTC 2 cut(s) 259, 515
AccIII TCCGGA 1 cut(s) 655
AciI CCGC 4 cut(s) 98, 257, 306, 513
AclWI GGATC 4 cut(s) 248, 358, 371, 762
AcoI YGGCCR 1 cut(s) 744
AcuI CTGAAG 1 cut(s) 461
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 5 cut(s) 340, 541, 622, 640, 735
AgeI ACCGGT 1 cut(s) 277
AgsI TTSAA 1 cut(s) 705
AjnI CCWGG 1 cut(s) 909
AloI GAACNNNNNNTCC 2 cut(s) 746, 778
AluBI AGCT 3 cut(s) 187, 371, 673
AluI AGCT 3 cut(s) 187, 371, 673
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 4 cut(s) 49, 176, 502, 600
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 4 cut(s) 248, 358, 371, 762
Ama87I CYCGRG 1 cut(s) 499
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 8 cut(s) 102, 403, 415, 480, 604, 625, 744, 858
ApaLI GTGCAC 1 cut(s) 770
ApeKI GCWGC 7 cut(s) 5, 35, 308, 376, 410, 452, 663
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
AsiGI ACCGGT 1 cut(s) 277
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 4 cut(s) 280, 604, 613, 625
AsuHPI GGTGA 4 cut(s) 87, 116, 267, 796
AvaI CYCGRG 1 cut(s) 499
AvaII GGWCC 2 cut(s) 280, 613
BaeGI GKGCMC 1 cut(s) 774
BalI TGGCCA 1 cut(s) 746
BamHI GGATCC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 7 cut(s) 17, 47, 295, 363, 397, 439, 650
BccI CCATC 2 cut(s) 533, 793
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 1 cut(s) 911
BcoDI GTCTC 4 cut(s) 49, 176, 502, 600
BfaI CTAG 2 cut(s) 11, 518
BfmI CTRYAG 5 cut(s) 33, 219, 411, 599, 617
BfoI RGCGCY 1 cut(s) 588
BisI GCNGC 7 cut(s) 6, 36, 309, 377, 411, 453, 664
BlsI GCNGC 7 cut(s) 7, 37, 310, 378, 412, 454, 665
Bme1390I CCNGG 1 cut(s) 911
Bme18I GGWCC 2 cut(s) 280, 613
BmeT110I CYCGRG 1 cut(s) 499
BmgT120I GGNCC 4 cut(s) 280, 604, 613, 625
BmiI GGNNCC 4 cut(s) 179, 365, 614, 907
BmrFI CCNGG 1 cut(s) 911
BmrI ACTGGG 2 cut(s) 59, 861
BmsI GCATC 1 cut(s) 634
BmuI ACTGGG 2 cut(s) 59, 861
BpmI CTGGAG 2 cut(s) 167, 284
BpuEI CTTGAG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 775
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 909
BsaWI WCCGGW 2 cut(s) 277, 655
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 330, 360
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 541, 622, 640, 735
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 5 cut(s) 45, 54, 301, 345, 867
Bse3DI GCAATG 2 cut(s) 198, 819
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 1 cut(s) 911
BseDI CCNNGG 1 cut(s) 909
BseGI GGATG 4 cut(s) 143, 467, 525, 649
BseLI CCNNNNNNNGG 5 cut(s) 340, 541, 622, 640, 735
BseMI GCAATG 2 cut(s) 198, 819
BseMII CTCAG 2 cut(s) 80, 187
BseNI ACTGG 5 cut(s) 45, 54, 301, 345, 867
BseRI GAGGAG 4 cut(s) 251, 323, 338, 341
BseSI GKGCMC 1 cut(s) 774
BseXI GCAGC 7 cut(s) 17, 47, 295, 363, 397, 439, 650
BseYI CCCAGC 1 cut(s) 367
BshFI GGCC 8 cut(s) 104, 405, 417, 482, 606, 627, 746, 860
BshNI GGYRCC 1 cut(s) 905
BshTI ACCGGT 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 774
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 2 cut(s) 278, 656
BslFI GGGAC 2 cut(s) 66, 516
BslI CCNNNNNNNGG 5 cut(s) 340, 541, 622, 640, 735
BsmAI GTCTC 4 cut(s) 49, 176, 502, 600
BsmBI CGTCTC 1 cut(s) 176
BsmFI GGGAC 2 cut(s) 66, 516
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 8 cut(s) 104, 405, 417, 482, 606, 627, 746, 860
Bso31I GGTCTC 1 cut(s) 49
BsoBI CYCGRG 1 cut(s) 499
Bsp1286I GDGCHC 1 cut(s) 774
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 4 cut(s) 92, 253, 363, 754
BspACI CCGC 4 cut(s) 98, 257, 306, 513
BspANI GGCC 8 cut(s) 104, 405, 417, 482, 606, 627, 746, 860
BspCNI CTCAG 2 cut(s) 81, 186
BspEI TCCGGA 1 cut(s) 655
BspLI GGNNCC 4 cut(s) 179, 365, 614, 907
BspMAI CTGCAG 2 cut(s) 37, 415
BspPI GGATC 4 cut(s) 248, 358, 371, 762
BspT107I GGYRCC 1 cut(s) 905
BspTNI GGTCTC 1 cut(s) 49
BsrBI CCGCTC 2 cut(s) 259, 515
BsrDI GCAATG 2 cut(s) 198, 819
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 5 cut(s) 45, 54, 301, 345, 867
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 909
BssMI GATC 4 cut(s) 92, 253, 363, 754
Bst2UI CCWGG 1 cut(s) 911
Bst4CI ACNGT 2 cut(s) 170, 460
Bst6I CTCTTC 2 cut(s) 323, 425
BstAPI GCANNNNNTGC 1 cut(s) 818
BstBAI YACGTR 1 cut(s) 775
BstC8I GCNNGC 4 cut(s) 102, 415, 547, 551
BstDEI CTNAG 5 cut(s) 89, 173, 244, 718, 915
BstEII GGTNACC 1 cut(s) 802
BstF5I GGATG 4 cut(s) 143, 467, 525, 649
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 4 cut(s) 95, 256, 366, 757
BstMAI GTCTC 4 cut(s) 49, 176, 502, 600
BstMBI GATC 4 cut(s) 92, 253, 363, 754
BstMWI GCNNNNNNNGC 2 cut(s) 110, 818
BstNI CCWGG 1 cut(s) 911
BstPI GGTNACC 1 cut(s) 802
BstSCI CCNGG 1 cut(s) 909
BstSFI CTRYAG 5 cut(s) 33, 219, 411, 599, 617
BstSLI GKGCMC 1 cut(s) 774
BstV1I GCAGC 7 cut(s) 17, 47, 295, 363, 397, 439, 650
BstX2I RGATCY 2 cut(s) 363, 754
BstYI RGATCY 2 cut(s) 363, 754
BsuRI GGCC 8 cut(s) 104, 405, 417, 482, 606, 627, 746, 860
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 4 cut(s) 143, 467, 525, 649
BtsIMutI CAGTG 5 cut(s) 38, 47, 338, 456, 874
Cac8I GCNNGC 4 cut(s) 102, 415, 547, 551
CfoI GCGC 1 cut(s) 587
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 4 cut(s) 280, 604, 613, 625
CseI GACGC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 16
CspAI ACCGGT 1 cut(s) 277
CviAII CATG 1 cut(s) 64
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 5 cut(s) 89, 173, 244, 718, 915
DpnI GATC 4 cut(s) 94, 255, 365, 756
DpnII GATC 4 cut(s) 92, 253, 363, 754
DrdI GACNNNNNNGTC 1 cut(s) 823
DseDI GACNNNNNNGTC 1 cut(s) 823
EaeI YGGCCR 1 cut(s) 744
Eam1104I CTCTTC 2 cut(s) 323, 425
EarI CTCTTC 2 cut(s) 323, 425
Eco147I AGGCCT 3 cut(s) 104, 417, 860
Eco31I GGTCTC 1 cut(s) 49
Eco32I GATATC 2 cut(s) 27, 510
Eco47I GGWCC 2 cut(s) 280, 613
Eco57I CTGAAG 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 499
Eco91I GGTNACC 1 cut(s) 802
EcoO109I RGGNCCY 2 cut(s) 604, 613
EcoO65I GGTNACC 1 cut(s) 802
EcoRII CCWGG 1 cut(s) 909
EcoRV GATATC 2 cut(s) 27, 510
Esp3I CGTCTC 1 cut(s) 176
FaeI CATG 1 cut(s) 67
FaiI YATR 5 cut(s) 65, 221, 610, 639, 810
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 2 cut(s) 66, 516
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 7 cut(s) 6, 36, 309, 377, 411, 453, 664
FokI GGATG 4 cut(s) 130, 454, 512, 656
Fsp4HI GCNGC 7 cut(s) 6, 36, 309, 377, 411, 453, 664
FspBI CTAG 2 cut(s) 11, 518
GlaI GCGC 1 cut(s) 586
GluI GCNGC 7 cut(s) 6, 36, 309, 377, 411, 453, 664
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 2 cut(s) 167, 284
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 8 cut(s) 104, 405, 417, 482, 606, 627, 746, 860
HapII CCGG 2 cut(s) 278, 656
HgaI GACGC 1 cut(s) 257
HhaI GCGC 1 cut(s) 587
Hin1II CATG 1 cut(s) 67
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 4 cut(s) 54, 491, 693, 725
HpaII CCGG 2 cut(s) 278, 656
HphI GGTGA 4 cut(s) 87, 116, 267, 796
Hpy166II GTNNAC 4 cut(s) 16, 226, 283, 772
Hpy188I TCNGA 3 cut(s) 358, 472, 888
Hpy188III TCNNGA 5 cut(s) 463, 501, 656, 677, 752
Hpy8I GTNNAC 4 cut(s) 16, 226, 283, 772
HpyAV CCTTC 2 cut(s) 485, 658
HpyCH4III ACNGT 2 cut(s) 170, 460
HpyCH4IV ACGT 2 cut(s) 573, 774
HpyCH4V TGCA 6 cut(s) 35, 203, 413, 430, 772, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 818
HpyF3I CTNAG 5 cut(s) 89, 173, 244, 718, 915
HpySE526I ACGT 2 cut(s) 573, 774
Hsp92II CATG 1 cut(s) 67
HspAI GCGC 1 cut(s) 585
Kpn2I TCCGGA 1 cut(s) 655
Kzo9I GATC 4 cut(s) 92, 253, 363, 754
LmnI GCTCC 1 cut(s) 264
Lsp1109I GCAGC 7 cut(s) 17, 47, 295, 363, 397, 439, 650
LweI GCATC 1 cut(s) 634
MaeI CTAG 2 cut(s) 11, 518
MaeII ACGT 2 cut(s) 573, 774
MaeIII GTNAC 4 cut(s) 488, 713, 802, 869
MalI GATC 4 cut(s) 94, 255, 365, 756
MbiI CCGCTC 2 cut(s) 259, 515
MboI GATC 4 cut(s) 92, 253, 363, 754
MboII GAAGA 3 cut(s) 310, 391, 412
MflI RGATCY 2 cut(s) 363, 754
MhlI GDGCHC 1 cut(s) 774
MlsI TGGCCA 1 cut(s) 746
MluNI TGGCCA 1 cut(s) 746
MlyI GAGTC 4 cut(s) 48, 485, 702, 734
MmeI TCCRAC 2 cut(s) 183, 495
Mox20I TGGCCA 1 cut(s) 746
MroI TCCGGA 1 cut(s) 655
MscI TGGCCA 1 cut(s) 746
MseI TTAA 1 cut(s) 734
MslI CAYNNNNRTG 1 cut(s) 887
Msp20I TGGCCA 1 cut(s) 746
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 278, 656
MspR9I CCNGG 1 cut(s) 911
Mva1269I GAATGC 1 cut(s) 203
MvaI CCWGG 1 cut(s) 911
MwoI GCNNNNNNNGC 2 cut(s) 110, 818
NdeII GATC 4 cut(s) 92, 253, 363, 754
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 4 cut(s) 179, 365, 614, 907
NmuCI GTSAC 4 cut(s) 488, 713, 802, 869
PceI AGGCCT 3 cut(s) 104, 417, 860
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PctI GAATGC 1 cut(s) 203
PflFI GACNNNGTC 1 cut(s) 494
PinAI ACCGGT 1 cut(s) 277
PkrI GCNGC 7 cut(s) 7, 37, 310, 378, 412, 454, 665
PleI GAGTC 4 cut(s) 48, 485, 701, 733
PpsI GAGTC 4 cut(s) 48, 485, 701, 733
Ppu21I YACGTR 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 909
PspEI GGTNACC 1 cut(s) 802
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 909
PspN4I GGNNCC 4 cut(s) 179, 365, 614, 907
PspPI GGNCC 4 cut(s) 280, 604, 613, 625
PspPPI RGGWCCY 1 cut(s) 613
PstI CTGCAG 2 cut(s) 37, 415
PsuI RGATCY 2 cut(s) 363, 754
PsyI GACNNNGTC 1 cut(s) 494
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 887
SaqAI TTAA 1 cut(s) 734
SatI GCNGC 7 cut(s) 6, 36, 309, 377, 411, 453, 664
Sau3AI GATC 4 cut(s) 92, 253, 363, 754
Sau96I GGNCC 4 cut(s) 280, 604, 613, 625
SchI GAGTC 4 cut(s) 48, 485, 702, 734
ScrFI CCNGG 1 cut(s) 911
SduI GDGCHC 1 cut(s) 774
SfaNI GCATC 1 cut(s) 634
SfcI CTRYAG 5 cut(s) 33, 219, 411, 599, 617
SinI GGWCC 2 cut(s) 280, 613
SmiMI CAYNNNNRTG 1 cut(s) 887
SmlI CTYRAG 2 cut(s) 263, 898
SmoI CTYRAG 2 cut(s) 263, 898
SseBI AGGCCT 3 cut(s) 104, 417, 860
SsiI CCGC 4 cut(s) 98, 257, 306, 513
SspMI CTAG 2 cut(s) 11, 518
StuI AGGCCT 3 cut(s) 104, 417, 860
StyD4I CCNGG 1 cut(s) 909
TaaI ACNGT 2 cut(s) 170, 460
TaiI ACGT 2 cut(s) 576, 777
TaqI TCGA 2 cut(s) 696, 838
Tru1I TTAA 1 cut(s) 734
Tru9I TTAA 1 cut(s) 734
TscAI CASTG 5 cut(s) 45, 54, 345, 463, 874
TseFI GTSAC 4 cut(s) 488, 713, 802, 869
TseI GCWGC 7 cut(s) 5, 35, 308, 376, 410, 452, 663
Tsp45I GTSAC 4 cut(s) 488, 713, 802, 869
TspDTI ATGAA 2 cut(s) 640, 905
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 5 cut(s) 45, 54, 345, 463, 874
Tth111I GACNNNGTC 1 cut(s) 494
VneI GTGCAC 1 cut(s) 770
VpaK11BI GGWCC 2 cut(s) 280, 613
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XspI CTAG 2 cut(s) 11, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.