AT5G14260

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008681
Physical Location & Seq
Forward (+)
4600904 .. 4604482
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14260.2

Sequence Viewer

Length: 1545 bp
ATGGAAGGGGTTATCACATGTTTCCATACGAAATGCGTCTCTCTTCCAATTCGTTCTTTCCCACTTTCTAGGGTTTCTTCACTTCCTCGGTGGAGGAACAATAAATTGATCTCTTCATCGCGATCGGTTCATCTAAGAAGCTTGTGCGTTTCGAGCTCAGATACTTTAGTCGCGAGCGGTTCACCAAAGGAAGACGAGAGGCAGAGCAAAGTATCGAGCAAGAAGGAAGGAGATGACTCCGAGGATTTGAAGTTTTGGATGGATAAGAATGGGTTGCCACCTTGCAAGGTTATTCTCAAGGAGAGACCAGCTCATGATCAGAAGCATAAACCTATACATTATGTTGCTGCTAGTGAGGATCTTCAGAAGGGTGATGTGGCGTTTTCAGTTCCTGATTCTCTGGTGGTTACTCTGGAAAGAGTTTTGGGAAACGAGACTATTGCTGAACTGTTGACGACGAACAAATTATCCGAACTGGCTTGTCTAGCTTTGTATTTGATGTATGAGAAGAAACAAGGAAAGAAATCAGTATGGTATCCCTATATAAGAGAACTTGATCGTCAAAGAGGAAGAGGTCAGTTAGATGCGGAATCCCCTTTGCTGTGGTCAGAGGCTGAGTTAGATTACCTAACTGGTAGTCCTACCAAGGCCGAAGTTCTTGAGAGGGCTGAAGGGATCAAAAGAGAATACAATGAGCTTGACACTGTCTGGTTCATGGCTGGATCTTTGTTTCAGCAATACCCATTTGACATACCAACTGAAGCTTTTTCATTTGAGATTTTCAAACAGGCATTTGTTGCCATTCAGTCATGTGTGGTGCATTTACAAAATGTCGGTTTGGCTCGTCGGTTTGCTTTGGTTCCTCTTGGTCCTCCCTTGTTGGCCTATTGTTCCAACTGCAAGGCAATGCTTACTGCTGTTGATGGTGCTGTTGAACTCGTGGTAGATAGGCCATATAAGGCCGGGGATCCTATAGTTGTCTGGTGTGGGCCACAACCAAATGCGAAATTGCTTCTGAACTATGGATTTGTTGATGAGGATAACCCTTATGATAGAGTAATAGTTGAGGCAGCGTTGAACACTGAGGATCCTCAATACCAGGACAAGAGAATGGTTGCTCAAAGGAACGGAAAATTATCCCAACAAGTATTTCAGGTTCGTGTGGGAAAAGAAAGAGAAGCTGTTCAAGACATGCTTCCCTACCTGCGGTTGGGTTACATGTCTGATCCTTCTGAAATGCAGTCAGTAATTTCATCCCAAGGTCCAGTTTGTCCGATGAGCCCTTGTATGGAAAGAGCAGTACTAGATCAGCTTGCTAATTACTTCATGAGACGGTTATCTGGCTACCCTACTACTCCGAAAGAAGATGATGCATTGTTGGCAGATCCTAGTTTGAGTCCTAGGAAGCGAGTTGCAACCCGGCTTGTCCAACTGGAGAAGAAAATACTAGTTGCATGCCTTACGACAACAGTTGATCTTTTAAATCAGTTGCCCGATACAGCCATCTCTCCATGCCCAGCACCATATGCTCCTTCCTTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind

Protein Analysis

514

Amino Acids

57.58

Weight (kDa)

6.38

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 361 - 483 7.7e-31 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1212
AccBSI CCGCTC 1 cut(s) 177
AccII CGCG 2 cut(s) 121, 173
AciI CCGC 3 cut(s) 177, 587, 1207
AclWI GGATC 9 cut(s) 366, 683, 730, 962, 975, 1082, 1095, 1220, 1379
AcuI CTGAAG 3 cut(s) 347, 690, 780
AfaI GTAC 1 cut(s) 1302
AfiI CCNNNNNNNGG 3 cut(s) 1206, 1210, 1288
AflIII ACRYGT 2 cut(s) 17, 1218
AgsI TTSAA 6 cut(s) 250, 784, 935, 1078, 1187, 1540
AhlI ACTAGT 1 cut(s) 1447
AjnI CCWGG 1 cut(s) 1098
AjuI GAANNNNNNNTTGG 2 cut(s) 1134, 1166
AloI GAACNNNNNNTCC 2 cut(s) 452, 484
AluBI AGCT 8 cut(s) 141, 156, 311, 488, 697, 764, 1181, 1312
AluI AGCT 8 cut(s) 141, 156, 311, 488, 697, 764, 1181, 1312
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 4 cut(s) 43, 298, 428, 1324
AlwI GGATC 9 cut(s) 366, 683, 730, 962, 975, 1082, 1095, 1220, 1379
AlwNI CAGNNNCTG 2 cut(s) 392, 614
AoxI GGCC 5 cut(s) 648, 882, 950, 960, 989
ApeKI GCWGC 2 cut(s) 347, 1070
Asp700I GAANNNNTTC 1 cut(s) 1182
AspA2I CCTAGG 1 cut(s) 1400
AspS9I GGNCC 3 cut(s) 869, 989, 1262
AsuC2I CCSGG 2 cut(s) 964, 1420
AsuHPI GGTGA 2 cut(s) 174, 383
AvaII GGWCC 2 cut(s) 869, 1262
AvrII CCTAGG 1 cut(s) 1400
BaeI ACNNNNGTAYC 2 cut(s) 153, 186
BamHI GGATCC 2 cut(s) 967, 1087
BanII GRGCYC 2 cut(s) 158, 1283
BauI CACGAG 1 cut(s) 938
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 158
BbvI GCAGC 2 cut(s) 334, 1082
BccI CCATC 3 cut(s) 253, 917, 1511
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 1 cut(s) 1100
BciVI GTATCC 1 cut(s) 546
BclI TGATCA 1 cut(s) 316
BcnI CCSGG 2 cut(s) 964, 1420
BcoDI GTCTC 4 cut(s) 43, 298, 428, 1324
BcuI ACTAGT 1 cut(s) 1447
BfaI CTAG 7 cut(s) 69, 351, 485, 1304, 1389, 1401, 1448
BfmI CTRYAG 1 cut(s) 972
BfuAI ACCTGC 1 cut(s) 1212
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 2 cut(s) 348, 1071
BlnI CCTAGG 1 cut(s) 1400
BlsI GCNGC 2 cut(s) 349, 1072
BmcAI AGTACT 1 cut(s) 1302
Bme1390I CCNGG 3 cut(s) 964, 1100, 1420
Bme18I GGWCC 2 cut(s) 869, 1262
BmgT120I GGNCC 3 cut(s) 869, 989, 1262
BmiI GGNNCC 3 cut(s) 861, 969, 1089
BmrFI CCNGG 3 cut(s) 964, 1100, 1420
BmsI GCATC 2 cut(s) 574, 1360
BpiI GAAGAC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpmI CTGGAG 1 cut(s) 1454
BpuEI CTTGAG 2 cut(s) 281, 680
BpuMI CCSGG 2 cut(s) 964, 1420
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 6 cut(s) 86, 240, 645, 963, 1258, 1400
Bsc4I CCNNNNNNNGG 3 cut(s) 1206, 1210, 1288
Bse1I ACTGG 4 cut(s) 480, 637, 1265, 1437
Bse3DI GCAATG 1 cut(s) 912
BseBI CCWGG 1 cut(s) 1100
BseDI CCNNGG 6 cut(s) 86, 240, 645, 963, 1258, 1400
BseGI GGATG 2 cut(s) 264, 1253
BseLI CCNNNNNNNGG 3 cut(s) 1206, 1210, 1288
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 3 cut(s) 171, 606, 1074
BseNI ACTGG 4 cut(s) 480, 637, 1265, 1437
BseXI GCAGC 2 cut(s) 334, 1082
BseYI CCCAGC 1 cut(s) 1516
Bsh1236I CGCG 2 cut(s) 121, 173
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 5 cut(s) 650, 884, 952, 962, 991
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 158
BsiSI CCGG 2 cut(s) 963, 1420
BslI CCNNNNNNNGG 3 cut(s) 1206, 1210, 1288
BsmAI GTCTC 4 cut(s) 43, 298, 428, 1324
BsmBI CGTCTC 2 cut(s) 43, 1324
BsnI GGCC 5 cut(s) 650, 884, 952, 962, 991
Bso31I GGTCTC 1 cut(s) 298
Bsp1286I GDGCHC 2 cut(s) 158, 1283
Bsp68I TCGCGA 2 cut(s) 121, 173
BspACI CCGC 3 cut(s) 177, 587, 1207
BspANI GGCC 5 cut(s) 650, 884, 952, 962, 991
BspCNI CTCAG 3 cut(s) 170, 607, 1075
BspFNI CGCG 2 cut(s) 121, 173
BspHI TCATGA 2 cut(s) 313, 1326
BspLI GGNNCC 3 cut(s) 861, 969, 1089
BspMI ACCTGC 1 cut(s) 1212
BspPI GGATC 9 cut(s) 366, 683, 730, 962, 975, 1082, 1095, 1220, 1379
BspTNI GGTCTC 1 cut(s) 298
BsrBI CCGCTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 4 cut(s) 480, 637, 1265, 1437
BssECI CCNNGG 6 cut(s) 86, 240, 645, 963, 1258, 1400
BssSI CACGAG 1 cut(s) 938
BssT1I CCWWGG 3 cut(s) 645, 1258, 1400
Bst2BI CACGAG 1 cut(s) 938
Bst2UI CCWGG 1 cut(s) 1100
Bst4CI ACNGT 4 cut(s) 450, 706, 1335, 1471
Bst6I CTCTTC 3 cut(s) 48, 118, 565
BstAPI GCANNNNNTGC 2 cut(s) 797, 1526
BstC8I GCNNGC 3 cut(s) 175, 1314, 1456
BstDEI CTNAG 4 cut(s) 134, 157, 615, 1083
BstF5I GGATG 2 cut(s) 264, 1253
BstFNI CGCG 2 cut(s) 121, 173
BstMAI GTCTC 4 cut(s) 43, 298, 428, 1324
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 5 cut(s) 153, 485, 797, 1379, 1526
BstNI CCWGG 1 cut(s) 1100
BstNSI RCATGY 4 cut(s) 21, 1195, 1222, 1458
BstSCI CCNGG 3 cut(s) 962, 1098, 1418
BstSFI CTRYAG 1 cut(s) 972
BstUI CGCG 2 cut(s) 121, 173
BstV1I GCAGC 2 cut(s) 334, 1082
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 5 cut(s) 358, 722, 967, 1087, 1384
BstYI RGATCY 5 cut(s) 358, 722, 967, 1087, 1384
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 5 cut(s) 650, 884, 952, 962, 991
BtgZI GCGATG 1 cut(s) 102
BtsCI GGATG 2 cut(s) 264, 1253
BtsIMutI CAGTG 2 cut(s) 702, 1080
BtuMI TCGCGA 2 cut(s) 121, 173
BveI ACCTGC 1 cut(s) 1212
Cac8I GCNNGC 3 cut(s) 175, 1314, 1456
CaiI CAGNNNCTG 2 cut(s) 392, 614
CciI TCATGA 2 cut(s) 313, 1326
Cfr13I GGNCC 3 cut(s) 869, 989, 1262
CseI GACGC 1 cut(s) 25
Csp6I GTAC 1 cut(s) 1301
CviAII CATG 9 cut(s) 18, 314, 715, 810, 1192, 1219, 1327, 1455, 1512
CviQI GTAC 1 cut(s) 1301
DdeI CTNAG 4 cut(s) 134, 157, 615, 1083
DraI TTTAAA 1 cut(s) 1482
Eam1104I CTCTTC 3 cut(s) 48, 118, 565
EarI CTCTTC 3 cut(s) 48, 118, 565
Ecl136II GAGCTC 1 cut(s) 156
Eco130I CCWWGG 3 cut(s) 645, 1258, 1400
Eco24I GRGCYC 2 cut(s) 158, 1283
Eco31I GGTCTC 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 869, 1262
Eco53kI GAGCTC 1 cut(s) 156
Eco57I CTGAAG 3 cut(s) 347, 690, 780
EcoICRI GAGCTC 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 1098
EcoT14I CCWWGG 3 cut(s) 645, 1258, 1400
EcoT22I ATGCAT 1 cut(s) 1375
EcoT38I GRGCYC 2 cut(s) 158, 1283
ErhI CCWWGG 3 cut(s) 645, 1258, 1400
Esp3I CGTCTC 2 cut(s) 43, 1324
FaeI CATG 9 cut(s) 21, 317, 718, 813, 1195, 1222, 1330, 1458, 1515
FalI AAGNNNNNCTT 2 cut(s) 1179, 1211
FatI CATG 9 cut(s) 17, 313, 714, 809, 1191, 1218, 1326, 1454, 1511
FauNDI CATATG 1 cut(s) 1525
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 2 cut(s) 348, 1071
FokI GGATG 2 cut(s) 271, 1240
FriOI GRGCYC 2 cut(s) 158, 1283
Fsp4HI GCNGC 2 cut(s) 348, 1071
FspBI CTAG 7 cut(s) 69, 351, 485, 1304, 1389, 1401, 1448
GluI GCNGC 2 cut(s) 348, 1071
GsaI CCCAGC 1 cut(s) 1520
GsuI CTGGAG 1 cut(s) 1454
HaeIII GGCC 5 cut(s) 650, 884, 952, 962, 991
HapII CCGG 2 cut(s) 963, 1420
HgaI GACGC 1 cut(s) 25
Hin1II CATG 9 cut(s) 21, 317, 718, 813, 1195, 1222, 1330, 1458, 1515
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HindIII AAGCTT 2 cut(s) 139, 762
HinfI GANTC 4 cut(s) 236, 395, 590, 1396
HpaII CCGG 2 cut(s) 963, 1420
HphI GGTGA 2 cut(s) 174, 383
Hpy166II GTNNAC 2 cut(s) 182, 453
Hpy188III TCNNGA 8 cut(s) 120, 172, 314, 392, 413, 659, 1187, 1327
Hpy8I GTNNAC 2 cut(s) 182, 453
Hpy99I CGWCG 2 cut(s) 460, 849
HpyAV CCTTC 6 cut(s) 217, 221, 361, 665, 1239, 1542
HpyCH4III ACNGT 4 cut(s) 450, 706, 1335, 1471
HpyCH4V TGCA 7 cut(s) 285, 820, 900, 1240, 1373, 1415, 1454
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 485, 797, 1379, 1526
HpyF3I CTNAG 4 cut(s) 134, 157, 615, 1083
Hsp92II CATG 9 cut(s) 21, 317, 718, 813, 1195, 1222, 1330, 1458, 1515
Ksp22I TGATCA 1 cut(s) 316
LmnI GCTCC 1 cut(s) 1534
Lsp1109I GCAGC 2 cut(s) 334, 1082
LweI GCATC 2 cut(s) 574, 1360
MaeI CTAG 7 cut(s) 69, 351, 485, 1304, 1389, 1401, 1448
MaeIII GTNAC 2 cut(s) 406, 1214
MbiI CCGCTC 1 cut(s) 177
MboII GAAGA 9 cut(s) 35, 69, 105, 203, 353, 520, 582, 1376, 1450
MflI RGATCY 5 cut(s) 358, 722, 967, 1087, 1384
MhlI GDGCHC 2 cut(s) 158, 1283
MluCI AATT 7 cut(s) 48, 104, 464, 1007, 1133, 1248, 1318
MlyI GAGTC 2 cut(s) 230, 1405
MmeI TCCRAC 2 cut(s) 918, 1453
Mph1103I ATGCAT 1 cut(s) 1375
MroXI GAANNNNTTC 1 cut(s) 1182
MseI TTAA 1 cut(s) 1481
MspI CCGG 2 cut(s) 963, 1420
MspR9I CCNGG 3 cut(s) 964, 1100, 1420
MvaI CCWGG 1 cut(s) 1100
MvnI CGCG 2 cut(s) 121, 173
MwoI GCNNNNNNNGC 5 cut(s) 153, 485, 797, 1379, 1526
NciI CCSGG 2 cut(s) 964, 1420
NdeI CATATG 1 cut(s) 1525
NlaIII CATG 9 cut(s) 21, 317, 718, 813, 1195, 1222, 1330, 1458, 1515
NlaIV GGNNCC 3 cut(s) 861, 969, 1089
NruI TCGCGA 2 cut(s) 121, 173
NsiI ATGCAT 1 cut(s) 1375
NspI RCATGY 4 cut(s) 21, 1195, 1222, 1458
PaeI GCATGC 1 cut(s) 1458
PagI TCATGA 2 cut(s) 313, 1326
PciI ACATGT 2 cut(s) 17, 1218
PdmI GAANNNNTTC 1 cut(s) 1182
PfeI GAWTC 2 cut(s) 395, 590
PflFI GACNNNGTC 1 cut(s) 704
PkrI GCNGC 2 cut(s) 349, 1072
Ple19I CGATCG 1 cut(s) 125
PleI GAGTC 2 cut(s) 230, 1404
PpsI GAGTC 2 cut(s) 230, 1404
PscI ACATGT 2 cut(s) 17, 1218
Psp124BI GAGCTC 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 1098
PspFI CCCAGC 1 cut(s) 1516
PspGI CCWGG 1 cut(s) 1098
PspN4I GGNNCC 3 cut(s) 861, 969, 1089
PspPI GGNCC 3 cut(s) 869, 989, 1262
PsrI GAACNNNNNNTAC 2 cut(s) 1140, 1172
PstNI CAGNNNCTG 2 cut(s) 392, 614
PsuI RGATCY 5 cut(s) 358, 722, 967, 1087, 1384
PsyI GACNNNGTC 1 cut(s) 704
PvuI CGATCG 1 cut(s) 125
RruI TCGCGA 2 cut(s) 121, 173
RsaI GTAC 1 cut(s) 1302
RsaNI GTAC 1 cut(s) 1301
SacI GAGCTC 1 cut(s) 158
SaqAI TTAA 1 cut(s) 1481
SatI GCNGC 2 cut(s) 348, 1071
Sau96I GGNCC 3 cut(s) 869, 989, 1262
ScaI AGTACT 1 cut(s) 1302
SchI GAGTC 2 cut(s) 230, 1405
ScrFI CCNGG 3 cut(s) 964, 1100, 1420
SduI GDGCHC 2 cut(s) 158, 1283
SfaNI GCATC 2 cut(s) 574, 1360
SfcI CTRYAG 1 cut(s) 972
SinI GGWCC 2 cut(s) 869, 1262
SmlI CTYRAG 2 cut(s) 296, 659
SmoI CTYRAG 2 cut(s) 296, 659
SpeI ACTAGT 1 cut(s) 1447
SphI GCATGC 1 cut(s) 1458
Sse9I AATT 7 cut(s) 48, 104, 464, 1007, 1133, 1248, 1318
SsiI CCGC 3 cut(s) 177, 587, 1207
SspMI CTAG 7 cut(s) 69, 351, 485, 1304, 1389, 1401, 1448
SstI GAGCTC 1 cut(s) 158
StyD4I CCNGG 3 cut(s) 962, 1098, 1418
StyI CCWWGG 3 cut(s) 645, 1258, 1400
TaaI ACNGT 4 cut(s) 450, 706, 1335, 1471
TaqI TCGA 2 cut(s) 152, 215
TasI AATT 7 cut(s) 48, 104, 464, 1007, 1133, 1248, 1318
TatI WGTACW 1 cut(s) 1300
TfiI GAWTC 2 cut(s) 395, 590
Tru1I TTAA 1 cut(s) 1481
Tru9I TTAA 1 cut(s) 1481
TscAI CASTG 2 cut(s) 709, 1087
TseI GCWGC 2 cut(s) 347, 1070
TspDTI ATGAA 6 cut(s) 105, 119, 703, 759, 1242, 1315
TspGWI ACGGA 1 cut(s) 1143
TspRI CASTG 2 cut(s) 709, 1087
Tth111I GACNNNGTC 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 869, 1262
XceI RCATGY 4 cut(s) 21, 1195, 1222, 1458
XmaJI CCTAGG 1 cut(s) 1400
XmnI GAANNNNTTC 1 cut(s) 1182
XspI CTAG 7 cut(s) 69, 351, 485, 1304, 1389, 1401, 1448
ZrmI AGTACT 1 cut(s) 1302
Zsp2I ATGCAT 1 cut(s) 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.