MD09G1015500.v1.1

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008681
Physical Location & Seq
Reverse (-)
955839 .. 959683
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1015500.v1.1.491

Sequence Viewer

Length: 1650 bp
ATGGAGCTCACTCCTCCGCCCCACCGTTGCATTTGGTCCTCGCCGATTCGTTCGTCCTGCTCCTCGCACCGCCCTCTCCTCGTTTCAATTTCGCCTCGGAAGACCAAGCTCCTTAGGTCCGTACCCTCTTTCAGATCGATTCGCCGGAGAAATTTGTGCTCCGCCTCCAGCTCCGAGACGTTGGTTGGGTCGCGGAAGGAGGACGGCGGCAAGGAGATTCAGGTGGTGAGCAAGAAAGACGAGGAGGAAGACTTGAAATCTTGGATGCACAAGAACGGCCTGCCTCCTTGCAAGGTCGTGCTCAAGGACAAACCTTCCTACGACGAGAAGCTCCGGCCCATTCATTACGTAGCTGCCAGCGAAGATCTTGAGGTTGGTGATGTCGCATTTTCGGTGCCGGATTCTTTAGTTGTGACTCTCAAGAGAGTGTTGGGGAACGAAACAATCGCTGAATTATTAACTACGAACAAATTATCGGAATTGGCATGCTTGGCATTGTATCTGATGTATGAGAAGAAACAAGGAAAGAAATCATTTTGGTATCCCTATATAAGAGAGCTCGATCGTCAGCGAGGGAGGGGTCAATTAGCGGTGGAATCGCCCCTTTTATGGTCAGAAGCTGAACTGGCCTATCTTACAGGCAGCCCCACAAAGGCTGACGTTCGTGCAAGGGCCGAAGGAATTAAAAGAGAATATAATGAGCTTGACACTGTCTGGTTTATGGCTGGTTCCCTATTTCAGCAATACCCATATGACATTCCCACAGAGGCCTTCCCATTTGAGATATTCAAACAAGCTTTTGTTGCAGTTCAGTCGTGTGTTGTGCATTTGCAGAAAGTTAGTTTGGCTCAAAGATTTGCGTTGGTTCCTCTTGGGCCCCCATTGTTAGCTTACGGAAGCAACTGTAGAGCAATGTTGACAGCTGTTGATGGTGCTGTTCAACTAGTGGTTGATCGGCCATATAAAGCAGGGGAGTCTATTGTTGTGTGGTGTGGACCACAGCCTAATTCAAAGTTGCTTATAAACTATGGTTTTGTTGATGAAGATAATCCTTATGACCGTTTGGTGGTTGAGGCAGCTTTGAACACTGAGGATCCACAATATCAAGATAAAAGAATGGTTGCTCAAAGAAATGGAAAATTATCAGTACAAACTTTTCAGGTATATGTGGGAAAGGAAAAGGAAACGGTCTTCGATATGCTTCCCTATCTGCGACTGGGCTATGTTTCAGATCCTTCAGAGATGCAGTCTGTTATCTCTTCTCAAGGTCCAATTTGTGCAGTGAGTCCTTGTATGGAACGAGCGGTGTTGGACCAACTTGCTGATTATTTTAGGACACGGCTGGCTGGCTATCCAACTACATTGAGCGAAGATGATTCTTTGTTGGCAGATGCTAATTTGAATCCAAAGAAGCGAGTTGCTACTCGGCTTGTTAGGTTAGAAAAGAAAATGCTCCATGCATGCCTGAAGGTGGTGGTTGATTTGATAGGCCAGTTACCAGACGACACTGTATCACCATGCCCAGCTCCCTATGCCCCCTCATGGATCGAAGGAAGCGGGCAACGCATAGGGCAACCAAGGAGAACTGGAATATGTCTCCGCCTCCATCAGGCATCAAAGAGGAGAGAATCAGGCATTCTTTTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind

Protein Analysis

550

Amino Acids

61.6

Weight (kDa)

8.7

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 363 - 485 1e-29 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
AccB1I GGYRCC 1 cut(s) 394
AccBSI CCGCTC 1 cut(s) 1304
AccII CGCG 1 cut(s) 193
AciI CCGC 9 cut(s) 17, 70, 162, 193, 207, 590, 1304, 1557, 1600
AclWI GGATC 4 cut(s) 1088, 1101, 1226, 1553
AcoI YGGCCR 1 cut(s) 956
AcsI RAATTY 1 cut(s) 151
AcuI CTGAAG 2 cut(s) 1221, 1487
AfaI GTAC 2 cut(s) 123, 1149
AfiI CCNNNNNNNGG 6 cut(s) 609, 652, 1066, 1471, 1542, 1609
AgsI TTSAA 7 cut(s) 87, 256, 790, 941, 1011, 1084, 1402
AhlI ACTAGT 1 cut(s) 943
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
Alw21I GWGCWC 4 cut(s) 9, 161, 303, 561
Alw26I GTCTC 2 cut(s) 170, 1601
AlwI GGATC 4 cut(s) 1088, 1101, 1226, 1553
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 8 cut(s) 277, 335, 627, 672, 768, 875, 956, 1489
ApaI GGGCCC 1 cut(s) 879
ApeKI GCWGC 3 cut(s) 353, 642, 1076
ApoI RAATTY 1 cut(s) 151
AspS9I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1312
AsuHPI GGTGA 3 cut(s) 238, 389, 1506
AvaII GGWCC 5 cut(s) 36, 117, 995, 1268, 1312
AxyI CCTNAGG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 879
BamHI GGATCC 1 cut(s) 1093
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 9, 561, 879
BbsI GAAGAC 3 cut(s) 107, 255, 1183
Bbv12I GWGCWC 4 cut(s) 9, 161, 303, 561
BbvI GCAGC 3 cut(s) 340, 654, 1088
BccI CCATC 2 cut(s) 923, 1614
BceAI ACGGC 3 cut(s) 220, 292, 1355
BcgI CGANNNNNNTGC 2 cut(s) 795, 829
BciVI GTATCC 1 cut(s) 552
BcoDI GTCTC 2 cut(s) 170, 1601
BcuI ACTAGT 1 cut(s) 943
BfaI CTAG 1 cut(s) 944
BfmI CTRYAG 1 cut(s) 904
BfuI GTATCC 1 cut(s) 552
BglII AGATCT 1 cut(s) 364
BisI GCNGC 4 cut(s) 208, 354, 643, 1077
BlsI GCNGC 4 cut(s) 209, 355, 644, 1078
Bme18I GGWCC 5 cut(s) 36, 117, 995, 1268, 1312
BmgT120I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1312
BmiI GGNNCC 6 cut(s) 396, 730, 867, 877, 878, 1095
BmrI ACTGGG 1 cut(s) 1226
BmsI GCATC 4 cut(s) 255, 1233, 1381, 1622
BmuI ACTGGG 1 cut(s) 1226
BpiI GAAGAC 3 cut(s) 107, 255, 1183
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 4 cut(s) 287, 389, 404, 1248
Bsa29I ATCGAT 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 349
BsaJI CCNNGG 2 cut(s) 95, 1577
Bsc4I CCNNNNNNNGG 6 cut(s) 609, 652, 1066, 1471, 1542, 1609
Bse1I ACTGG 4 cut(s) 630, 1221, 1492, 1591
Bse21I CCTNAGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 918
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 95, 1577
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 6 cut(s) 609, 652, 1066, 1471, 1542, 1609
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 1 cut(s) 1080
BseNI ACTGG 4 cut(s) 630, 1221, 1492, 1591
BseRI GAGGAG 4 cut(s) 52, 68, 257, 1636
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 3 cut(s) 340, 654, 1088
BseYI CCCAGC 1 cut(s) 1522
BsgI GTGCAG 1 cut(s) 1299
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 8 cut(s) 279, 337, 629, 674, 770, 877, 958, 1491
BshNI GGYRCC 1 cut(s) 394
BshVI ATCGAT 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 565
BsiHKAI GWGCWC 4 cut(s) 9, 161, 303, 561
BsiSI CCGG 3 cut(s) 145, 334, 398
BslI CCNNNNNNNGG 6 cut(s) 609, 652, 1066, 1471, 1542, 1609
BsmAI GTCTC 2 cut(s) 170, 1601
BsmBI CGTCTC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 1635
BsnI GGCC 8 cut(s) 279, 337, 629, 674, 770, 877, 958, 1491
Bsp120I GGGCCC 1 cut(s) 875
Bsp1286I GDGCHC 5 cut(s) 9, 161, 303, 561, 879
Bsp143I GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
BspACI CCGC 9 cut(s) 17, 70, 162, 193, 207, 590, 1304, 1557, 1600
BspANI GGCC 8 cut(s) 279, 337, 629, 674, 770, 877, 958, 1491
BspCNI CTCAG 1 cut(s) 1081
BspDI ATCGAT 1 cut(s) 137
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 6 cut(s) 396, 730, 867, 877, 878, 1095
BspPI GGATC 4 cut(s) 1088, 1101, 1226, 1553
BspT107I GGYRCC 1 cut(s) 394
BsrBI CCGCTC 1 cut(s) 1304
BsrDI GCAATG 1 cut(s) 918
BsrI ACTGG 4 cut(s) 630, 1221, 1492, 1591
BssECI CCNNGG 2 cut(s) 95, 1577
BssMI GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
BssT1I CCWWGG 1 cut(s) 1577
Bst4CI ACNGT 6 cut(s) 26, 712, 905, 1061, 1189, 1510
Bst6I CTCTTC 1 cut(s) 1264
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 7 cut(s) 281, 358, 487, 1344, 1348, 1462, 1559
BstDEI CTNAG 2 cut(s) 113, 1089
BstENI CCTNNNNNAGG 1 cut(s) 1607
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 7 cut(s) 137, 367, 565, 955, 1096, 1234, 1548
BstMAI GTCTC 2 cut(s) 170, 1601
BstMBI GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 5 cut(s) 491, 626, 803, 1532, 1563
BstNSI RCATGY 2 cut(s) 489, 1464
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 1 cut(s) 879
BstSNI TACGTA 1 cut(s) 349
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 3 cut(s) 340, 654, 1088
BstV2I GAAGAC 3 cut(s) 107, 255, 1183
BstX2I RGATCY 3 cut(s) 364, 1093, 1231
BstYI RGATCY 3 cut(s) 364, 1093, 1231
Bsu15I ATCGAT 1 cut(s) 137
Bsu36I CCTNAGG 1 cut(s) 113
BsuI GTATCC 1 cut(s) 552
BsuRI GGCC 8 cut(s) 279, 337, 629, 674, 770, 877, 958, 1491
BsuTUI ATCGAT 1 cut(s) 137
BtsCI GGATG 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 1287
BtsIMutI CAGTG 4 cut(s) 708, 1086, 1287, 1506
Cac8I GCNNGC 7 cut(s) 281, 358, 487, 1344, 1348, 1462, 1559
CaiI CAGNNNCTG 1 cut(s) 620
Cfr13I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1312
ClaI ATCGAT 1 cut(s) 137
Csp6I GTAC 2 cut(s) 122, 1148
CviAII CATG 5 cut(s) 486, 1457, 1461, 1518, 1542
CviQI GTAC 2 cut(s) 122, 1148
DdeI CTNAG 2 cut(s) 113, 1089
DpnI GATC 7 cut(s) 136, 366, 564, 954, 1095, 1233, 1547
DpnII GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
EaeI YGGCCR 1 cut(s) 956
Eam1104I CTCTTC 1 cut(s) 1264
EarI CTCTTC 1 cut(s) 1264
EciI GGCGGA 3 cut(s) 6, 151, 1589
Ecl136II GAGCTC 2 cut(s) 7, 559
Eco105I TACGTA 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 1577
Eco147I AGGCCT 1 cut(s) 770
Eco24I GRGCYC 3 cut(s) 9, 561, 879
Eco47I GGWCC 5 cut(s) 36, 117, 995, 1268, 1312
Eco53kI GAGCTC 2 cut(s) 7, 559
Eco57I CTGAAG 2 cut(s) 1221, 1487
Eco81I CCTNAGG 1 cut(s) 113
EcoICRI GAGCTC 2 cut(s) 7, 559
EcoNI CCTNNNNNAGG 1 cut(s) 1607
EcoO109I RGGNCCY 1 cut(s) 876
EcoT14I CCWWGG 1 cut(s) 1577
EcoT22I ATGCAT 1 cut(s) 1462
EcoT38I GRGCYC 3 cut(s) 9, 561, 879
ErhI CCWWGG 1 cut(s) 1577
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 5 cut(s) 489, 1460, 1464, 1521, 1545
FatI CATG 5 cut(s) 485, 1456, 1460, 1517, 1541
FauI CCCGC 1 cut(s) 1550
FauNDI CATATG 1 cut(s) 751
Fnu4HI GCNGC 4 cut(s) 208, 354, 643, 1077
FokI GGATG 1 cut(s) 277
FriOI GRGCYC 3 cut(s) 9, 561, 879
Fsp4HI GCNGC 4 cut(s) 208, 354, 643, 1077
FspBI CTAG 1 cut(s) 944
GluI GCNGC 4 cut(s) 208, 354, 643, 1077
GsaI CCCAGC 1 cut(s) 1526
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 8 cut(s) 279, 337, 629, 674, 770, 877, 958, 1491
HapII CCGG 3 cut(s) 145, 334, 398
Hin1II CATG 5 cut(s) 489, 1460, 1464, 1521, 1545
HincII GTYRAC 1 cut(s) 918
HindII GTYRAC 1 cut(s) 918
HindIII AAGCTT 1 cut(s) 795
HpaII CCGG 3 cut(s) 145, 334, 398
HphI GGTGA 3 cut(s) 238, 389, 1506
Hpy166II GTNNAC 2 cut(s) 918, 995
Hpy188I TCNGA 8 cut(s) 99, 134, 175, 478, 504, 616, 1231, 1240
Hpy188III TCNNGA 3 cut(s) 368, 421, 1106
Hpy8I GTNNAC 2 cut(s) 918, 995
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 7 cut(s) 190, 324, 671, 781, 1245, 1462, 1544
HpyCH4III ACNGT 6 cut(s) 26, 712, 905, 1061, 1189, 1510
HpyCH4IV ACGT 3 cut(s) 179, 348, 660
HpyF10VI GCNNNNNNNGC 5 cut(s) 491, 626, 803, 1532, 1563
HpyF3I CTNAG 2 cut(s) 113, 1089
HpySE526I ACGT 3 cut(s) 179, 348, 660
Hsp92II CATG 5 cut(s) 489, 1460, 1464, 1521, 1545
Kzo9I GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
LmnI GCTCC 8 cut(s) 4, 65, 114, 164, 176, 336, 1458, 1531
Lsp1109I GCAGC 3 cut(s) 340, 654, 1088
LweI GCATC 4 cut(s) 255, 1233, 1381, 1622
MaeI CTAG 1 cut(s) 944
MaeII ACGT 3 cut(s) 179, 348, 660
MaeIII GTNAC 2 cut(s) 412, 1494
MalI GATC 7 cut(s) 136, 366, 564, 954, 1095, 1233, 1547
MbiI CCGCTC 1 cut(s) 1304
MboI GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
MboII GAAGA 8 cut(s) 112, 260, 374, 526, 1055, 1183, 1251, 1382
MflI RGATCY 3 cut(s) 364, 1093, 1231
MhlI GDGCHC 5 cut(s) 9, 161, 303, 561, 879
MlyI GAGTC 3 cut(s) 409, 983, 1294
MmeI TCCRAC 2 cut(s) 1290, 1379
Mph1103I ATGCAT 1 cut(s) 1462
MseI TTAA 2 cut(s) 458, 684
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 3 cut(s) 145, 334, 398
Mva1269I GAATGC 1 cut(s) 1635
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 5 cut(s) 491, 626, 803, 1532, 1563
NdeI CATATG 1 cut(s) 751
NdeII GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
NlaIII CATG 5 cut(s) 489, 1460, 1464, 1521, 1545
NlaIV GGNNCC 6 cut(s) 396, 730, 867, 877, 878, 1095
NmeAIII GCCGAG 1 cut(s) 1405
NmuCI GTSAC 1 cut(s) 412
NsiI ATGCAT 1 cut(s) 1462
NspI RCATGY 2 cut(s) 489, 1464
PaeI GCATGC 2 cut(s) 489, 1464
PceI AGGCCT 1 cut(s) 770
PctI GAATGC 1 cut(s) 1635
PfeI GAWTC 8 cut(s) 46, 139, 217, 401, 596, 1376, 1402, 1628
PflFI GACNNNGTC 1 cut(s) 710
PkrI GCNGC 4 cut(s) 209, 355, 644, 1078
Ple19I CGATCG 1 cut(s) 565
PleI GAGTC 3 cut(s) 409, 982, 1293
PpsI GAGTC 3 cut(s) 409, 982, 1293
Ppu21I YACGTR 1 cut(s) 349
PsiI TTATAA 1 cut(s) 1022
Psp124BI GAGCTC 2 cut(s) 9, 561
PspFI CCCAGC 1 cut(s) 1522
PspN4I GGNNCC 6 cut(s) 396, 730, 867, 877, 878, 1095
PspOMI GGGCCC 1 cut(s) 875
PspPI GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1312
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 364, 1093, 1231
PsyI GACNNNGTC 1 cut(s) 710
PvuI CGATCG 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 923
RsaI GTAC 2 cut(s) 123, 1149
RsaNI GTAC 2 cut(s) 122, 1148
SacI GAGCTC 2 cut(s) 9, 561
SaqAI TTAA 2 cut(s) 458, 684
SatI GCNGC 4 cut(s) 208, 354, 643, 1077
Sau3AI GATC 7 cut(s) 134, 364, 562, 952, 1093, 1231, 1545
Sau96I GGNCC 9 cut(s) 36, 117, 336, 672, 875, 876, 995, 1268, 1312
SchI GAGTC 3 cut(s) 409, 983, 1294
SduI GDGCHC 5 cut(s) 9, 161, 303, 561, 879
SfaNI GCATC 4 cut(s) 255, 1233, 1381, 1622
SfcI CTRYAG 1 cut(s) 904
SinI GGWCC 5 cut(s) 36, 117, 995, 1268, 1312
SmlI CTYRAG 4 cut(s) 302, 368, 419, 1263
SmoI CTYRAG 4 cut(s) 302, 368, 419, 1263
SnaBI TACGTA 1 cut(s) 349
SpeI ACTAGT 1 cut(s) 943
SphI GCATGC 2 cut(s) 489, 1464
SseBI AGGCCT 1 cut(s) 770
SsiI CCGC 9 cut(s) 17, 70, 162, 193, 207, 590, 1304, 1557, 1600
SspMI CTAG 1 cut(s) 944
SstI GAGCTC 2 cut(s) 9, 561
StuI AGGCCT 1 cut(s) 770
StyI CCWWGG 1 cut(s) 1577
TaaI ACNGT 6 cut(s) 26, 712, 905, 1061, 1189, 1510
TaiI ACGT 3 cut(s) 182, 351, 663
TaqI TCGA 4 cut(s) 137, 561, 1194, 1548
TatI WGTACW 1 cut(s) 1147
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 8 cut(s) 46, 139, 217, 401, 596, 1376, 1402, 1628
Tru1I TTAA 2 cut(s) 458, 684
Tru9I TTAA 2 cut(s) 458, 684
TscAI CASTG 4 cut(s) 715, 1093, 1287, 1513
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 3 cut(s) 353, 642, 1076
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 332, 1056
TspGWI ACGGA 2 cut(s) 109, 909
TspRI CASTG 4 cut(s) 715, 1093, 1287, 1513
Tth111I GACNNNGTC 1 cut(s) 710
VpaK11BI GGWCC 5 cut(s) 36, 117, 995, 1268, 1312
XagI CCTNNNNNAGG 1 cut(s) 1607
XapI RAATTY 1 cut(s) 151
XceI RCATGY 2 cut(s) 489, 1464
XspI CTAG 1 cut(s) 944
Zsp2I ATGCAT 1 cut(s) 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.