Rh2DG684700

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008681
Physical Location & Seq
Forward (+)
90345737 .. 90349474
3738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG684700.1

Sequence Viewer

Length: 1287 bp
ATGGAGGTCATTCCTCTACACCACAGCTGCACCTGGCTCCCGATTCTCTCCCGCGGCTCATCGATTTCGCGGCGGAGGAGTTATCCCAGGGTTTCGCCTGGATCCATTCGCCGGAGAAATGTGTGCCTGGCCTCCGGCTCCGAGACCTTGATCGGGTCACGTAAGGAGGAAGGCAAGCGTCGCAAGGCCGTGAACGAGAGAGACGAGGCCGAGGACTTGAAATCGTGGATGCACAAGAATGGGCTGCCGCCGTGCAAGGTTGTGCTCAAGGAACGACCGTCGTATGATGAGAAGCACAGCGCTATTCACTACGTAGCTGCTAGTGAAGATCTTGAGGTTGGTGATGTTGCATTTGCGGTGCCGAATTCGCTAGTTGTGACTCTGAAGAGAGTGTTGGGGAACGAAACTATAGCGGAATTGTTAACTACAAACAAGTTATCCGAATTGGCATGCTTGGCACTCTATTTGATGTATGAGAAGAAACAAGGGAAGAAGTCATTTTGGTATCCGTACATCAGAGAACTTGATCGTCAGCGAGGGAGAGGTCAGTTAGCGGTGGAGTCTCCCCTCTTATGGTCGGAGACCGAGCTGGCTTATCTTACCGGCAGCCCCACAAAGGCTGAAGTTCAAGAAAGGGCTGAAGGAATTAAAAGGGAATATAATGAGCTTGACACTGTCTGGTTTATGGCTGGGTCCCTATTTCAGCAATACCCATACGATATTCCTACTGAAGCCTTCCCGTTTGAGATATTCAAACAAGCTTTTGTTGCAGTTCAGTCTTGTGTAGTGCATCTGCAGAAAGTTAGTCTGGCTCAGAGGTTTGCTTTGGTACCTCTTGGACCCCCATTGTTAGCTTACAAAAGTAACTGCAAGGCAATGTTGACAGCTACTGATGATGCGGTTCAACTAGTGGTTGATCGGCCATATAAGGCAGGGGAATCTATTGCTGTTTGGTGTGGGCCACAGCCTAATTCAAAATTGCTTATAAATTACGGTTTTGTTGACGAAGATAATTCTTATGACCGTTTGATGGTTGAGGCAGCTTTGAATACTGAGGATCCACAATATCAAGATAAAAGAATGGTTGCTCAGAGAAATGGAAAATTGTCAGTACAAGCTTTTCAGGTATATGTCGGGAAGGAAAAGGAAACTGTCTCGGATATGCTTCCCTATCTGCGACTAGGCTATGTTTCAGATCCTTCGGAGATGCAGTCTGTTATCTCTTCTCAAGGTCCAATTTGTCCAGTAAGTTCAACTTTTAGGATAAGAACACATGCTTATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind

Protein Analysis

428

Amino Acids

48.39

Weight (kDa)

8.26

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 351 - 418 2.8e-10 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 986
Acc65I GGTACC 1 cut(s) 829
AccB1I GGYRCC 2 cut(s) 358, 829
AccII CGCG 2 cut(s) 54, 70
AciI CCGC 9 cut(s) 52, 54, 70, 73, 248, 356, 413, 554, 899
AclWI GGATC 5 cut(s) 96, 109, 1052, 1065, 1190
AcoI YGGCCR 1 cut(s) 920
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 4 cut(s) 404, 642, 660, 750
AfaI GTAC 3 cut(s) 512, 831, 1113
AfeI AGCGCT 1 cut(s) 301
AfiI CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
AgsI TTSAA 7 cut(s) 220, 629, 754, 905, 975, 1048, 1254
AhlI ACTAGT 1 cut(s) 907
AjnI CCWGG 4 cut(s) 32, 86, 97, 126
AjuI GAANNNNNNNTTGG 2 cut(s) 377, 409
AluBI AGCT 9 cut(s) 27, 317, 589, 667, 761, 854, 887, 1043, 1118
AluI AGCT 9 cut(s) 27, 317, 589, 667, 761, 854, 887, 1043, 1118
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 5 cut(s) 137, 195, 567, 575, 1159
AlwI GGATC 5 cut(s) 96, 109, 1052, 1065, 1190
AlwNI CAGNNNCTG 1 cut(s) 890
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 5 cut(s) 129, 186, 207, 920, 959
ApeKI GCWGC 5 cut(s) 27, 244, 317, 606, 1040
ApoI RAATTY 1 cut(s) 364
Asp718I GGTACC 1 cut(s) 829
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 4 cut(s) 693, 839, 959, 1232
AsuHPI GGTGA 1 cut(s) 353
AvaII GGWCC 3 cut(s) 693, 839, 1232
BamHI GGATCC 2 cut(s) 101, 1057
BanI GGYRCC 2 cut(s) 358, 829
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 5 cut(s) 14, 231, 304, 618, 1052
BccI CCATC 1 cut(s) 1024
BceAI ACGGC 2 cut(s) 173, 235
BciT130I CCWGG 4 cut(s) 34, 88, 99, 128
BciVI GTATCC 1 cut(s) 516
BcoDI GTCTC 5 cut(s) 137, 195, 567, 575, 1159
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 4 cut(s) 321, 371, 908, 1181
BfmI CTRYAG 2 cut(s) 408, 794
BfoI RGCGCY 1 cut(s) 303
BfuI GTATCC 1 cut(s) 516
BglII AGATCT 1 cut(s) 328
BisI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
BlsI GCNGC 8 cut(s) 29, 56, 72, 246, 249, 319, 608, 1042
Bme1390I CCNGG 4 cut(s) 34, 88, 99, 128
Bme18I GGWCC 3 cut(s) 693, 839, 1232
BmgT120I GGNCC 4 cut(s) 693, 839, 959, 1232
BmiI GGNNCC 9 cut(s) 38, 103, 139, 360, 694, 695, 831, 841, 1059
BmrFI CCNGG 4 cut(s) 34, 88, 99, 128
BmsI GCATC 4 cut(s) 219, 799, 886, 1197
BpuEI CTTGAG 3 cut(s) 251, 353, 1212
Bsa29I ATCGAT 1 cut(s) 62
BsaAI YACGTR 2 cut(s) 161, 313
BsaI GGTCTC 2 cut(s) 137, 575
BsaJI CCNNGG 4 cut(s) 52, 86, 87, 210
BsaXI ACNNNNNCTCC 2 cut(s) 1196, 1226
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 1 cut(s) 1244
Bse3DI GCAATG 1 cut(s) 882
BseBI CCWGG 4 cut(s) 34, 88, 99, 128
BseCI ATCGAT 1 cut(s) 62
BseDI CCNNGG 4 cut(s) 52, 86, 87, 210
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 3 cut(s) 827, 1044, 1103
BseNI ACTGG 1 cut(s) 1244
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 5 cut(s) 14, 231, 304, 618, 1052
BseYI CCCAGC 1 cut(s) 689
BsgI GTGCAG 1 cut(s) 13
Bsh1236I CGCG 2 cut(s) 54, 70
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 5 cut(s) 131, 188, 209, 922, 961
BshNI GGYRCC 2 cut(s) 358, 829
BshVI ATCGAT 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 3 cut(s) 112, 135, 603
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
BsmAI GTCTC 5 cut(s) 137, 195, 567, 575, 1159
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 679
BsnI GGCC 5 cut(s) 131, 188, 209, 922, 961
Bso31I GGTCTC 2 cut(s) 137, 575
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
BspACI CCGC 9 cut(s) 52, 54, 70, 73, 248, 356, 413, 554, 899
BspANI GGCC 5 cut(s) 131, 188, 209, 922, 961
BspCNI CTCAG 3 cut(s) 826, 1045, 1102
BspDI ATCGAT 1 cut(s) 62
BspFNI CGCG 2 cut(s) 54, 70
BspLI GGNNCC 9 cut(s) 38, 103, 139, 360, 694, 695, 831, 841, 1059
BspMAI CTGCAG 1 cut(s) 798
BspPI GGATC 5 cut(s) 96, 109, 1052, 1065, 1190
BspT107I GGYRCC 2 cut(s) 358, 829
BspTNI GGTCTC 2 cut(s) 137, 575
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 1 cut(s) 602
BsrI ACTGG 1 cut(s) 1244
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 4 cut(s) 52, 86, 87, 210
BssMI GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
Bst2UI CCWGG 4 cut(s) 34, 88, 99, 128
Bst4CI ACNGT 5 cut(s) 279, 676, 995, 1025, 1153
Bst6I CTCTTC 2 cut(s) 380, 1228
BstBAI YACGTR 2 cut(s) 161, 313
BstC8I GCNNGC 3 cut(s) 176, 451, 591
BstDEI CTNAG 3 cut(s) 813, 1053, 1089
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 1 cut(s) 234
BstFNI CGCG 2 cut(s) 54, 70
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 7 cut(s) 104, 153, 331, 529, 919, 1060, 1198
BstMAI GTCTC 5 cut(s) 137, 195, 567, 575, 1159
BstMBI GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 4 cut(s) 180, 367, 455, 767
BstNI CCWGG 4 cut(s) 34, 88, 99, 128
BstNSI RCATGY 2 cut(s) 453, 1277
BstSCI CCNGG 4 cut(s) 32, 86, 97, 126
BstSFI CTRYAG 2 cut(s) 408, 794
BstSNI TACGTA 1 cut(s) 313
BstUI CGCG 2 cut(s) 54, 70
BstV1I GCAGC 5 cut(s) 14, 231, 304, 618, 1052
BstX2I RGATCY 4 cut(s) 101, 328, 1057, 1195
BstYI RGATCY 4 cut(s) 101, 328, 1057, 1195
Bsu15I ATCGAT 1 cut(s) 62
BsuI GTATCC 1 cut(s) 516
BsuRI GGCC 5 cut(s) 131, 188, 209, 922, 961
BsuTUI ATCGAT 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 672
Cac8I GCNNGC 3 cut(s) 176, 451, 591
CaiI CAGNNNCTG 1 cut(s) 890
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 4 cut(s) 693, 839, 959, 1232
Cfr42I CCGCGG 1 cut(s) 55
ClaI ATCGAT 1 cut(s) 62
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 511, 830, 1112
CviAII CATG 2 cut(s) 450, 1274
CviQI GTAC 3 cut(s) 511, 830, 1112
DdeI CTNAG 3 cut(s) 813, 1053, 1089
DpnI GATC 7 cut(s) 103, 152, 330, 528, 918, 1059, 1197
DpnII GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
EaeI YGGCCR 1 cut(s) 920
Eam1104I CTCTTC 2 cut(s) 380, 1228
EarI CTCTTC 2 cut(s) 380, 1228
EciI GGCGGA 1 cut(s) 88
Eco105I TACGTA 1 cut(s) 313
Eco31I GGTCTC 2 cut(s) 137, 575
Eco47I GGWCC 3 cut(s) 693, 839, 1232
Eco47III AGCGCT 1 cut(s) 301
Eco57I CTGAAG 4 cut(s) 404, 642, 660, 750
EcoO109I RGGNCCY 1 cut(s) 693
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 4 cut(s) 32, 86, 97, 126
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 2 cut(s) 453, 1277
FalI AAGNNNNNCTT 2 cut(s) 1240, 1272
FaqI GGGAC 1 cut(s) 679
FatI CATG 2 cut(s) 449, 1273
FauI CCCGC 1 cut(s) 59
Fnu4HI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
FokI GGATG 1 cut(s) 241
Fsp4HI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
FspBI CTAG 4 cut(s) 321, 371, 908, 1181
GlaI GCGC 1 cut(s) 301
GluI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
GsaI CCCAGC 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 5 cut(s) 131, 188, 209, 922, 961
HapII CCGG 3 cut(s) 112, 135, 603
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 302
Hin1II CATG 2 cut(s) 453, 1277
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 3 cut(s) 423, 882, 1003
HindII GTYRAC 3 cut(s) 423, 882, 1003
HindIII AAGCTT 2 cut(s) 759, 1116
HinfI GANTC 4 cut(s) 43, 379, 560, 938
HpaI GTTAAC 1 cut(s) 423
HpaII CCGG 3 cut(s) 112, 135, 603
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 4 cut(s) 193, 423, 882, 1003
Hpy188III TCNNGA 5 cut(s) 40, 332, 629, 1070, 1135
Hpy8I GTNNAC 4 cut(s) 193, 423, 882, 1003
Hpy99I CGWCG 2 cut(s) 183, 283
HpyAV CCTTC 5 cut(s) 164, 635, 745, 1132, 1209
HpyCH4III ACNGT 5 cut(s) 279, 676, 995, 1025, 1153
HpyCH4IV ACGT 2 cut(s) 160, 312
HpyCH4V TGCA 9 cut(s) 30, 232, 255, 350, 770, 790, 796, 870, 1210
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 367, 455, 767
HpyF3I CTNAG 3 cut(s) 813, 1053, 1089
HpySE526I ACGT 2 cut(s) 160, 312
Hsp92II CATG 2 cut(s) 453, 1277
HspAI GCGC 1 cut(s) 300
KflI GGGWCCC 1 cut(s) 693
KpnI GGTACC 1 cut(s) 833
KspAI GTTAAC 1 cut(s) 423
KspI CCGCGG 1 cut(s) 55
Kzo9I GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
LmnI GCTCC 2 cut(s) 42, 143
Lsp1109I GCAGC 5 cut(s) 14, 231, 304, 618, 1052
LweI GCATC 4 cut(s) 219, 799, 886, 1197
MaeI CTAG 4 cut(s) 321, 371, 908, 1181
MaeII ACGT 2 cut(s) 160, 312
MaeIII GTNAC 3 cut(s) 156, 376, 863
MalI GATC 7 cut(s) 103, 152, 330, 528, 918, 1059, 1197
MboI GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
MboII GAAGA 6 cut(s) 338, 397, 490, 502, 1019, 1215
MflI RGATCY 4 cut(s) 101, 328, 1057, 1195
MhlI GDGCHC 1 cut(s) 267
MlyI GAGTC 2 cut(s) 373, 569
MmeI TCCRAC 1 cut(s) 558
MseI TTAA 3 cut(s) 422, 648, 1285
MslI CAYNNNNRTG 1 cut(s) 237
MspA1I CMGCKG 2 cut(s) 27, 54
MspI CCGG 3 cut(s) 112, 135, 603
MspR9I CCNGG 4 cut(s) 34, 88, 99, 128
MvaI CCWGG 4 cut(s) 34, 88, 99, 128
MvnI CGCG 2 cut(s) 54, 70
MwoI GCNNNNNNNGC 4 cut(s) 180, 367, 455, 767
NdeII GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
NlaIII CATG 2 cut(s) 453, 1277
NlaIV GGNNCC 9 cut(s) 38, 103, 139, 360, 694, 695, 831, 841, 1059
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 156, 376
NspI RCATGY 2 cut(s) 453, 1277
PaeI GCATGC 1 cut(s) 453
PasI CCCWGGG 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 2 cut(s) 43, 938
PflFI GACNNNGTC 1 cut(s) 674
PkrI GCNGC 8 cut(s) 29, 56, 72, 246, 249, 319, 608, 1042
PleI GAGTC 2 cut(s) 373, 568
PpsI GAGTC 2 cut(s) 373, 568
Ppu21I YACGTR 2 cut(s) 161, 313
PpuMI RGGWCCY 1 cut(s) 693
PsiI TTATAA 1 cut(s) 986
Psp5II RGGWCCY 1 cut(s) 693
Psp6I CCWGG 4 cut(s) 32, 86, 97, 126
PspFI CCCAGC 1 cut(s) 689
PspGI CCWGG 4 cut(s) 32, 86, 97, 126
PspN4I GGNNCC 9 cut(s) 38, 103, 139, 360, 694, 695, 831, 841, 1059
PspPI GGNCC 4 cut(s) 693, 839, 959, 1232
PspPPI RGGWCCY 1 cut(s) 693
PstI CTGCAG 1 cut(s) 798
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 4 cut(s) 101, 328, 1057, 1195
PsyI GACNNNGTC 1 cut(s) 674
PvuII CAGCTG 1 cut(s) 27
RsaI GTAC 3 cut(s) 512, 831, 1113
RsaNI GTAC 3 cut(s) 511, 830, 1112
RseI CAYNNNNRTG 1 cut(s) 237
SacII CCGCGG 1 cut(s) 55
SaqAI TTAA 3 cut(s) 422, 648, 1285
SatI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
Sau3AI GATC 7 cut(s) 101, 150, 328, 526, 916, 1057, 1195
Sau96I GGNCC 4 cut(s) 693, 839, 959, 1232
SchI GAGTC 2 cut(s) 373, 569
ScrFI CCNGG 4 cut(s) 34, 88, 99, 128
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 4 cut(s) 219, 799, 886, 1197
SfcI CTRYAG 2 cut(s) 408, 794
Sfr303I CCGCGG 1 cut(s) 55
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 3 cut(s) 693, 839, 1232
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 3 cut(s) 266, 332, 1227
SmoI CTYRAG 3 cut(s) 266, 332, 1227
SnaBI TACGTA 1 cut(s) 313
SpeI ACTAGT 1 cut(s) 907
SphI GCATGC 1 cut(s) 453
SsiI CCGC 9 cut(s) 52, 54, 70, 73, 248, 356, 413, 554, 899
SspMI CTAG 4 cut(s) 321, 371, 908, 1181
StyD4I CCNGG 4 cut(s) 32, 86, 97, 126
TaaI ACNGT 5 cut(s) 279, 676, 995, 1025, 1153
TaiI ACGT 2 cut(s) 163, 315
TaqI TCGA 1 cut(s) 62
TaqII GACCGA 1 cut(s) 599
TatI WGTACW 1 cut(s) 1111
TauI GCSGC 3 cut(s) 57, 73, 250
TfiI GAWTC 2 cut(s) 43, 938
Tru1I TTAA 3 cut(s) 422, 648, 1285
Tru9I TTAA 3 cut(s) 422, 648, 1285
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 2 cut(s) 156, 376
TseI GCWGC 5 cut(s) 27, 244, 317, 606, 1040
Tsp45I GTSAC 2 cut(s) 156, 376
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 1 cut(s) 679
Tth111I GACNNNGTC 1 cut(s) 674
VpaK11BI GGWCC 3 cut(s) 693, 839, 1232
XapI RAATTY 1 cut(s) 364
XceI RCATGY 2 cut(s) 453, 1277
XspI CTAG 4 cut(s) 321, 371, 908, 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.