Rroxscaffold_2G00077790

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008681
Physical Location & Seq
Reverse (-)
1266899 .. 1270711
3813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077790.1

Sequence Viewer

Length: 1515 bp
ATGGAGGTCATTCCTCTACACCACAGCTGCACCTGGCTCCCGATTCTTTCCCGCGGCTCCTCGATTTCGCGGCGGAGGAGTTATTCCAGGGTTTCGCCTGGATCCATTCGCCGGAGAAATGTGTGCCTGGCCTCCGGCTCCGAGACCTTGATCGGGTCACGTAAGGAGGAAGGCAAGCGTCGCAAGGCCGTGAACGAGAGAGACGAGGCCGAGGACTTGAAATCGTGGATGCACAAGAATGGGCTGCCGCCGTGCAAGGTTGTGCTCAACGAACGACCGTCGTATGATGAGAAGCACAGCGCTATTCACTACGTAGCTGCTAGTGAAGATCTTGAGGTTGGTGATGTTGCATTTGCGGTGCCGAACTCGCTAGTTGTGACTCTGAAGAGAGTGTTGGGGAACGAAACTATAGCGGAATTGTTAACTACAAACAAGTTATCCGAATTGGCATGCTTGGCACTCTATTTGATGTATGAGAAGAAACAAGGGAAGAAGTCATTTTGGTATCCGTACATCAGAGAACTTGATCGTCAGCGAGGGAGAGGTCAGTTAGCGGTGGAGTCTCCCCTCTTATGGTCGGAGACCGAGCTGGCTTATCTTACCGGCAGCCCCACAAAGGCTGAAGTTCATGAAAGGGCTGAAGGAATTAAAAGAGAATATAATGAGCTTGACACTGTCTGGTTTATGGCTGGATCCCTATTTCAGCAATACCCATACGATATTCCTACTGAAGCCTTCCCGTTTGAGATATTCAAACAAGCTTTTGTTGCAGTTCAGTCTTGTGTAGTGCATCTGCAGAAAGTTAGTTTGGCTCAGAGGTTTGCTTTGGTACCTCTTGGACCCCCTTTGTTAGCTTACAAAAGCAACTGCAAGGCAATGTTGACAGCTACTGATGATGCGGTTCAACTAGTGGTTGATCGGCCATATAAGGCAGGGGAATCTATTGCTGTTTGGTGTGGGCCACAGCCTAATTCAAAATTGCTTATAAATTATGGTTTTGTTGACGAAGATAATTCTTATGACCGTTTGATGGTTGAGGCAGCTTTGAATACTGAGGATCCACAATATCAAGATAAAAGAATGGTTGCTCAGAGAAATGGAAAATTATCAGTACAAGCTTTTCAGGTATATGTCGGCAAGGAAAAGGAAACTGTCTCGGATATGCTTCCCTATCTGCGACTAGGCTATGTTTCAGATCCTTCGGAGATGCAGTCTGTTATCTCTTCTCAAGGTCCAATTTGTCCAGTTAGTCCCTGCATGGAAAGTGCAGTTTTGGACCAACTTGCTCAATATTTTAGAAGTCGCCTGGCTGGCTATCCAACTACTCTGAGTGAAGATGAGTCTTTGTTGGCGGATCCTAATTTGAATCCAAAGAAGCGAGTTGCTACACAGCTTGTTAGGTCAGAAAAGAAAATACTACATGCATGCCTGCAGGTGACTCTTGATTTTATAAATCAGTTACCCGATCTCAGTGTAACTCCATGCCCTGCCCCTTACGCCCCTTCGTTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind

Protein Analysis

504

Amino Acids

56.63

Weight (kDa)

7.06

Isoelectric Point (pI)

55.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 351 - 473 1.4e-32 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 986, 1451
AarI CACCTGC 1 cut(s) 1423
Acc36I ACCTGC 1 cut(s) 1423
Acc65I GGTACC 1 cut(s) 829
AccB1I GGYRCC 2 cut(s) 358, 829
AccII CGCG 2 cut(s) 54, 70
AclWI GGATC 9 cut(s) 96, 109, 687, 700, 1052, 1065, 1190, 1349, 1362
AcoI YGGCCR 1 cut(s) 920
AcuI CTGAAG 4 cut(s) 404, 642, 660, 750
AfaI GTAC 3 cut(s) 512, 831, 1113
AfeI AGCGCT 1 cut(s) 301
AfiI CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
AgsI TTSAA 6 cut(s) 220, 754, 905, 975, 1048, 1366
AhlI ACTAGT 1 cut(s) 907
AjnI CCWGG 5 cut(s) 32, 86, 97, 126, 1305
AjuI GAANNNNNNNTTGG 4 cut(s) 377, 409, 791, 823
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 5 cut(s) 137, 195, 567, 575, 1159
AlwI GGATC 9 cut(s) 96, 109, 687, 700, 1052, 1065, 1190, 1349, 1362
AlwNI CAGNNNCTG 1 cut(s) 890
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 5 cut(s) 129, 186, 207, 920, 959
ApeKI GCWGC 5 cut(s) 27, 244, 317, 606, 1040
Asp718I GGTACC 1 cut(s) 829
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 4 cut(s) 839, 959, 1232, 1276
AsuHPI GGTGA 2 cut(s) 353, 1447
AvaII GGWCC 3 cut(s) 839, 1232, 1276
BamHI GGATCC 4 cut(s) 101, 692, 1057, 1354
BanI GGYRCC 2 cut(s) 358, 829
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 5 cut(s) 14, 231, 304, 618, 1052
BccI CCATC 1 cut(s) 1024
BceAI ACGGC 2 cut(s) 173, 235
BciT130I CCWGG 5 cut(s) 34, 88, 99, 128, 1307
BciVI GTATCC 1 cut(s) 516
BcoDI GTCTC 5 cut(s) 137, 195, 567, 575, 1159
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 4 cut(s) 321, 371, 908, 1181
BfmI CTRYAG 3 cut(s) 408, 794, 1430
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 1423
BfuI GTATCC 1 cut(s) 516
BglI GCCNNNNNGGC 1 cut(s) 1311
BglII AGATCT 1 cut(s) 328
BisI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
BlsI GCNGC 8 cut(s) 29, 56, 72, 246, 249, 319, 608, 1042
Bme1390I CCNGG 5 cut(s) 34, 88, 99, 128, 1307
Bme18I GGWCC 3 cut(s) 839, 1232, 1276
BmgT120I GGNCC 4 cut(s) 839, 959, 1232, 1276
BmrFI CCNGG 5 cut(s) 34, 88, 99, 128, 1307
BmsI GCATC 4 cut(s) 219, 799, 886, 1197
BpuEI CTTGAG 2 cut(s) 353, 1212
BsaAI YACGTR 2 cut(s) 161, 313
BsaI GGTCTC 2 cut(s) 137, 575
BsaJI CCNNGG 3 cut(s) 52, 87, 210
BsaXI ACNNNNNCTCC 2 cut(s) 1196, 1226
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 1 cut(s) 1244
Bse3DI GCAATG 1 cut(s) 882
BseBI CCWGG 5 cut(s) 34, 88, 99, 128, 1307
BseDI CCNNGG 3 cut(s) 52, 87, 210
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 5 cut(s) 827, 1044, 1103, 1319, 1483
BseNI ACTGG 1 cut(s) 1244
BseRI GAGGAG 2 cut(s) 49, 91
BseXI GCAGC 5 cut(s) 14, 231, 304, 618, 1052
BsgI GTGCAG 2 cut(s) 13, 1287
Bsh1236I CGCG 2 cut(s) 54, 70
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 5 cut(s) 131, 188, 209, 922, 961
BshNI GGYRCC 2 cut(s) 358, 829
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 3 cut(s) 112, 135, 603
BslFI GGGAC 1 cut(s) 1236
BslI CCNNNNNNNGG 5 cut(s) 111, 153, 573, 616, 1030
BsmAI GTCTC 5 cut(s) 137, 195, 567, 575, 1159
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 1236
BsnI GGCC 5 cut(s) 131, 188, 209, 922, 961
Bso31I GGTCTC 2 cut(s) 137, 575
Bsp1286I GDGCHC 1 cut(s) 267
BspANI GGCC 5 cut(s) 131, 188, 209, 922, 961
BspCNI CTCAG 5 cut(s) 826, 1045, 1102, 1320, 1482
BspFNI CGCG 2 cut(s) 54, 70
BspHI TCATGA 1 cut(s) 628
BspMAI CTGCAG 2 cut(s) 798, 1434
BspMI ACCTGC 1 cut(s) 1423
BspPI GGATC 9 cut(s) 96, 109, 687, 700, 1052, 1065, 1190, 1349, 1362
BspT107I GGYRCC 2 cut(s) 358, 829
BspTNI GGTCTC 2 cut(s) 137, 575
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 1 cut(s) 602
BsrI ACTGG 1 cut(s) 1244
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 3 cut(s) 52, 87, 210
Bst2UI CCWGG 5 cut(s) 34, 88, 99, 128, 1307
Bst4CI ACNGT 4 cut(s) 279, 676, 1025, 1153
Bst6I CTCTTC 2 cut(s) 380, 1228
BstBAI YACGTR 2 cut(s) 161, 313
BstC8I GCNNGC 6 cut(s) 176, 451, 591, 1312, 1426, 1430
BstDEI CTNAG 5 cut(s) 813, 1053, 1089, 1328, 1469
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 1 cut(s) 234
BstFNI CGCG 2 cut(s) 54, 70
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstMAI GTCTC 5 cut(s) 137, 195, 567, 575, 1159
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 6 cut(s) 180, 367, 455, 767, 1311, 1496
BstNI CCWGG 5 cut(s) 34, 88, 99, 128, 1307
BstNSI RCATGY 3 cut(s) 453, 1424, 1428
BstSCI CCNGG 5 cut(s) 32, 86, 97, 126, 1305
BstSFI CTRYAG 3 cut(s) 408, 794, 1430
BstSNI TACGTA 1 cut(s) 313
BstUI CGCG 2 cut(s) 54, 70
BstV1I GCAGC 5 cut(s) 14, 231, 304, 618, 1052
BstX2I RGATCY 6 cut(s) 101, 328, 692, 1057, 1195, 1354
BstYI RGATCY 6 cut(s) 101, 328, 692, 1057, 1195, 1354
BsuI GTATCC 1 cut(s) 516
BsuRI GGCC 5 cut(s) 131, 188, 209, 922, 961
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 1 cut(s) 234
BtsIMutI CAGTG 2 cut(s) 672, 1477
BveI ACCTGC 1 cut(s) 1423
Cac8I GCNNGC 6 cut(s) 176, 451, 591, 1312, 1426, 1430
CaiI CAGNNNCTG 1 cut(s) 890
CciI TCATGA 1 cut(s) 628
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 4 cut(s) 839, 959, 1232, 1276
Cfr42I CCGCGG 1 cut(s) 55
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 511, 830, 1112
CviAII CATG 6 cut(s) 450, 629, 1258, 1421, 1425, 1482
CviQI GTAC 3 cut(s) 511, 830, 1112
DdeI CTNAG 5 cut(s) 813, 1053, 1089, 1328, 1469
EaeI YGGCCR 1 cut(s) 920
Eam1104I CTCTTC 2 cut(s) 380, 1228
EarI CTCTTC 2 cut(s) 380, 1228
EciI GGCGGA 2 cut(s) 88, 1367
Eco105I TACGTA 1 cut(s) 313
Eco31I GGTCTC 2 cut(s) 137, 575
Eco47I GGWCC 3 cut(s) 839, 1232, 1276
Eco47III AGCGCT 1 cut(s) 301
Eco57I CTGAAG 4 cut(s) 404, 642, 660, 750
EcoRII CCWGG 5 cut(s) 32, 86, 97, 126, 1305
EcoT22I ATGCAT 1 cut(s) 1426
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 6 cut(s) 453, 632, 1261, 1424, 1428, 1485
FaqI GGGAC 1 cut(s) 1236
FatI CATG 6 cut(s) 449, 628, 1257, 1420, 1424, 1481
FauI CCCGC 1 cut(s) 59
Fnu4HI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
FokI GGATG 1 cut(s) 241
Fsp4HI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
FspBI CTAG 4 cut(s) 321, 371, 908, 1181
GlaI GCGC 1 cut(s) 301
GluI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 5 cut(s) 131, 188, 209, 922, 961
HapII CCGG 3 cut(s) 112, 135, 603
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 302
Hin1II CATG 6 cut(s) 453, 632, 1261, 1424, 1428, 1485
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 3 cut(s) 423, 882, 1003
HindII GTYRAC 3 cut(s) 423, 882, 1003
HindIII AAGCTT 2 cut(s) 759, 1116
HinfI GANTC 7 cut(s) 43, 379, 560, 938, 1340, 1366, 1438
HpaI GTTAAC 1 cut(s) 423
HpaII CCGG 3 cut(s) 112, 135, 603
HphI GGTGA 2 cut(s) 353, 1447
Hpy166II GTNNAC 4 cut(s) 193, 423, 882, 1003
Hpy188III TCNNGA 5 cut(s) 40, 332, 629, 1070, 1442
Hpy8I GTNNAC 4 cut(s) 193, 423, 882, 1003
Hpy99I CGWCG 2 cut(s) 183, 283
HpyAV CCTTC 5 cut(s) 164, 635, 745, 1209, 1512
HpyCH4III ACNGT 4 cut(s) 279, 676, 1025, 1153
HpyCH4IV ACGT 2 cut(s) 160, 312
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 367, 455, 767, 1311, 1496
HpyF3I CTNAG 5 cut(s) 813, 1053, 1089, 1328, 1469
HpySE526I ACGT 2 cut(s) 160, 312
Hsp92II CATG 6 cut(s) 453, 632, 1261, 1424, 1428, 1485
HspAI GCGC 1 cut(s) 300
KpnI GGTACC 1 cut(s) 833
KspAI GTTAAC 1 cut(s) 423
KspI CCGCGG 1 cut(s) 55
LmnI GCTCC 3 cut(s) 42, 62, 143
Lsp1109I GCAGC 5 cut(s) 14, 231, 304, 618, 1052
LweI GCATC 4 cut(s) 219, 799, 886, 1197
MaeI CTAG 4 cut(s) 321, 371, 908, 1181
MaeII ACGT 2 cut(s) 160, 312
MaeIII GTNAC 5 cut(s) 156, 376, 1435, 1458, 1474
MboII GAAGA 7 cut(s) 338, 397, 490, 502, 1019, 1215, 1346
MflI RGATCY 6 cut(s) 101, 328, 692, 1057, 1195, 1354
MhlI GDGCHC 1 cut(s) 267
MlyI GAGTC 4 cut(s) 373, 569, 1349, 1432
MmeI TCCRAC 2 cut(s) 558, 1343
Mph1103I ATGCAT 1 cut(s) 1426
MseI TTAA 2 cut(s) 422, 648
MslI CAYNNNNRTG 1 cut(s) 237
MspA1I CMGCKG 2 cut(s) 27, 54
MspI CCGG 3 cut(s) 112, 135, 603
MspR9I CCNGG 5 cut(s) 34, 88, 99, 128, 1307
MvaI CCWGG 5 cut(s) 34, 88, 99, 128, 1307
MvnI CGCG 2 cut(s) 54, 70
MwoI GCNNNNNNNGC 6 cut(s) 180, 367, 455, 767, 1311, 1496
NlaIII CATG 6 cut(s) 453, 632, 1261, 1424, 1428, 1485
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 3 cut(s) 156, 376, 1435
NsiI ATGCAT 1 cut(s) 1426
NspI RCATGY 3 cut(s) 453, 1424, 1428
PaeI GCATGC 2 cut(s) 453, 1428
PagI TCATGA 1 cut(s) 628
PaqCI CACCTGC 1 cut(s) 1423
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 3 cut(s) 43, 938, 1366
PflFI GACNNNGTC 1 cut(s) 674
PkrI GCNGC 8 cut(s) 29, 56, 72, 246, 249, 319, 608, 1042
PleI GAGTC 4 cut(s) 373, 568, 1348, 1432
PpsI GAGTC 4 cut(s) 373, 568, 1348, 1432
Ppu21I YACGTR 2 cut(s) 161, 313
PsiI TTATAA 2 cut(s) 986, 1451
Psp6I CCWGG 5 cut(s) 32, 86, 97, 126, 1305
PspGI CCWGG 5 cut(s) 32, 86, 97, 126, 1305
PspPI GGNCC 4 cut(s) 839, 959, 1232, 1276
PstI CTGCAG 2 cut(s) 798, 1434
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 6 cut(s) 101, 328, 692, 1057, 1195, 1354
PsyI GACNNNGTC 1 cut(s) 674
PvuII CAGCTG 1 cut(s) 27
RsaI GTAC 3 cut(s) 512, 831, 1113
RsaNI GTAC 3 cut(s) 511, 830, 1112
RseI CAYNNNNRTG 1 cut(s) 237
SacII CCGCGG 1 cut(s) 55
SaqAI TTAA 2 cut(s) 422, 648
SatI GCNGC 8 cut(s) 28, 55, 71, 245, 248, 318, 607, 1041
Sau96I GGNCC 4 cut(s) 839, 959, 1232, 1276
SbfI CCTGCAGG 1 cut(s) 1434
SchI GAGTC 4 cut(s) 373, 569, 1349, 1432
ScrFI CCNGG 5 cut(s) 34, 88, 99, 128, 1307
SdaI CCTGCAGG 1 cut(s) 1434
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 4 cut(s) 219, 799, 886, 1197
SfcI CTRYAG 3 cut(s) 408, 794, 1430
Sfr303I CCGCGG 1 cut(s) 55
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 3 cut(s) 839, 1232, 1276
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 2 cut(s) 332, 1227
SmoI CTYRAG 2 cut(s) 332, 1227
SnaBI TACGTA 1 cut(s) 313
SpeI ACTAGT 1 cut(s) 907
SphI GCATGC 2 cut(s) 453, 1428
Sse8387I CCTGCAGG 1 cut(s) 1434
SspI AATATT 1 cut(s) 1292
SspMI CTAG 4 cut(s) 321, 371, 908, 1181
StyD4I CCNGG 5 cut(s) 32, 86, 97, 126, 1305
TaaI ACNGT 4 cut(s) 279, 676, 1025, 1153
TaiI ACGT 2 cut(s) 163, 315
TaqI TCGA 1 cut(s) 62
TaqII GACCGA 1 cut(s) 599
TatI WGTACW 1 cut(s) 1111
TauI GCSGC 3 cut(s) 57, 73, 250
TfiI GAWTC 3 cut(s) 43, 938, 1366
Tru1I TTAA 2 cut(s) 422, 648
Tru9I TTAA 2 cut(s) 422, 648
TscAI CASTG 2 cut(s) 679, 1477
TseFI GTSAC 3 cut(s) 156, 376, 1435
TseI GCWGC 5 cut(s) 27, 244, 317, 606, 1040
Tsp45I GTSAC 3 cut(s) 156, 376, 1435
TspDTI ATGAA 2 cut(s) 617, 645
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 2 cut(s) 679, 1477
Tth111I GACNNNGTC 1 cut(s) 674
VpaK11BI GGWCC 3 cut(s) 839, 1232, 1276
XceI RCATGY 3 cut(s) 453, 1424, 1428
XspI CTAG 4 cut(s) 321, 371, 908, 1181
Zsp2I ATGCAT 1 cut(s) 1426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.