MD17G1015800.v1.1

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008681
Physical Location & Seq
Reverse (-)
1275583 .. 1277758
2176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1015800.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGAGCTCAGTCCTCCGCACCACCGGTGCATTTGCTTGGAGACTGTTCGCTCGTCCTCCTCCTCTCACCGTCCTCTACTCGTTTCAATTTCGCCTCGGAAGACCAGGCTCCTCAGGTCCGTACCTTCTTTCAGATCGATTCGCCGGAGAAATCTGTGCTCCGCCTCCAGCTCCGAGACGTTGGTTGGGTCGCGGAAGGAGGACGGCGGCAAGCACAGTCAGGTCATGAGCAAGAAAGACGAGGAGGAAGACTTGAAATCTTGGATGCACAAGAACGGGCTGCCTCCTTGCAAGGTCGTGCTCAAGGAGAAACCTTCCTACGACGAGAAGCTCCGGCCCATTCATTACGTTGCTGCAAGCGAAGATCTTGAGGTTGGTGATGTTGCATTTTCGGTGCCGGATTCCTTAGTTGTGACTCTGAAGAGAGTGTTAGGGAACGAAACACTTGCTGAATTATTAACTACGAACAAATTATCGCAATTGGCATGCTTGGCATTGTATCTGATGTATGAGAAGAAACAAGGAAAGAAGTCATTTTGGTATCCCTATATAAGAGAGCTTGATCGTCAGCGAGGGAGGGGTCAGTTAGCGGTGGAGTCACCCCTTTTATGGTCAGAGGCGGAACTGGCTTATCTTACAGGAAGCCCCACAAAGGCTGATGTTCATGCAAGGGCCGAAGGAATTAAAAGGGAATATAACGAGCTTGACACGGTCTGGTTTATGGCTGGTTCCCTATTTCAGCAATACCCATATGACATTCCTACGGATGCCTTCCCTTTTGAGATATTCAAACAAGCTTTTGTTGCGGTTCAGTCTTGTGTGGTGCATTTGCAGAAAGTTAGTTTGGCTCAAAGATTTGCTTTGGTTCCTCTTGGGCCCCCATTGTTAGCTTATGGAAGCAACTGTAGGGCAATGTTGACAGCTATTGATGGCGCTGTTCAACTAGTGGTCGATAGGCCATATAAAGCAGGGGAGTCTATTGCTGTTTGGTGTGGACCACAGCCCAATTCAAAGTTGCTTATAAACTATGGTTTTGTTGATGAAGATAATTCTTATGACCGTTTGGTGGTTGAGGCAGCTTTGAATACCGAGGATCCACAATACCAAGATAAAAGAATGGTTGCGCAAGGAAATGGAAAACTATCGGTACAAACTTTTCAGGTATACGCGGGAAAGGAAAAGGAAACAGTCTTCGATATGCTTCCCTATCTGCGACTGGGCTATGTTTCAGATCCTTCAGAGATGCAGTCTGTTATCTCTTCTCAAGGTCCAATTTGTCCGGTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind

Protein Analysis

430

Amino Acids

48.09

Weight (kDa)

7.97

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 363 - 429 1.7e-09 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
Acc16I TGCGCA 1 cut(s) 1125
AccB1I GGYRCC 1 cut(s) 394
AccI GTMKAC 1 cut(s) 1164
AccII CGCG 2 cut(s) 193, 1169
AciI CCGC 8 cut(s) 17, 162, 193, 207, 590, 620, 806, 1169
AclWI GGATC 3 cut(s) 1088, 1101, 1226
AcuI CTGAAG 2 cut(s) 440, 1221
AfaI GTAC 2 cut(s) 123, 1149
AfiI CCNNNNNNNGG 3 cut(s) 609, 652, 1066
AgeI ACCGGT 1 cut(s) 24
AgsI TTSAA 6 cut(s) 87, 256, 790, 941, 1011, 1084
AhlI ACTAGT 1 cut(s) 943
AjnI CCWGG 1 cut(s) 104
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
AluBI AGCT 9 cut(s) 7, 171, 331, 559, 703, 797, 890, 923, 1079
AluI AGCT 9 cut(s) 7, 171, 331, 559, 703, 797, 890, 923, 1079
Alw21I GWGCWC 3 cut(s) 9, 161, 303
Alw26I GTCTC 2 cut(s) 35, 170
AlwI GGATC 3 cut(s) 1088, 1101, 1226
AoxI GGCC 4 cut(s) 335, 672, 875, 956
ApaI GGGCCC 1 cut(s) 879
ApeKI GCWGC 3 cut(s) 280, 353, 1076
AsiGI ACCGGT 1 cut(s) 24
AspLEI GCGC 2 cut(s) 935, 1126
AspS9I GGNCC 7 cut(s) 117, 336, 672, 875, 876, 995, 1268
AsuHPI GGTGA 3 cut(s) 59, 389, 591
AvaII GGWCC 3 cut(s) 117, 995, 1268
AxyI CCTNAGG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 879
BamHI GGATCC 1 cut(s) 1093
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 9, 879
BbsI GAAGAC 3 cut(s) 107, 255, 1183
Bbv12I GWGCWC 3 cut(s) 9, 161, 303
BbvI GCAGC 3 cut(s) 267, 340, 1088
BccI CCATC 1 cut(s) 923
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 1 cut(s) 106
BciVI GTATCC 1 cut(s) 552
BcoDI GTCTC 2 cut(s) 35, 170
BcuI ACTAGT 1 cut(s) 943
BfaI CTAG 1 cut(s) 944
BfmI CTRYAG 1 cut(s) 904
BfoI RGCGCY 1 cut(s) 936
BfuI GTATCC 1 cut(s) 552
BglII AGATCT 1 cut(s) 364
BisI GCNGC 4 cut(s) 208, 281, 354, 1077
BlsI GCNGC 4 cut(s) 209, 282, 355, 1078
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 3 cut(s) 117, 995, 1268
BmgT120I GGNCC 7 cut(s) 117, 336, 672, 875, 876, 995, 1268
BmiI GGNNCC 7 cut(s) 110, 396, 730, 867, 877, 878, 1095
BmrFI CCNGG 1 cut(s) 106
BmrI ACTGGG 1 cut(s) 1226
BmsI GCATC 3 cut(s) 255, 757, 1233
BmuI ACTGGG 1 cut(s) 1226
BpiI GAAGAC 3 cut(s) 107, 255, 1183
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 3 cut(s) 287, 389, 1248
Bsa29I ATCGAT 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 95, 1089
BsaWI WCCGGW 2 cut(s) 24, 1279
Bsc4I CCNNNNNNNGG 3 cut(s) 609, 652, 1066
Bse118I RCCGGY 1 cut(s) 24
Bse1I ACTGG 2 cut(s) 630, 1221
Bse21I CCTNAGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 1 cut(s) 106
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 95, 1089
BseGI GGATG 2 cut(s) 270, 772
BseLI CCNNNNNNNGG 3 cut(s) 609, 652, 1066
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 2 cut(s) 22, 127
BseNI ACTGG 2 cut(s) 630, 1221
BseRI GAGGAG 4 cut(s) 49, 52, 101, 257
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 3 cut(s) 267, 340, 1088
Bsh1236I CGCG 2 cut(s) 193, 1169
BshFI GGCC 4 cut(s) 337, 674, 877, 958
BshNI GGYRCC 1 cut(s) 394
BshTI ACCGGT 1 cut(s) 24
BshVI ATCGAT 1 cut(s) 137
BsiHKAI GWGCWC 3 cut(s) 9, 161, 303
BsiSI CCGG 5 cut(s) 25, 145, 334, 398, 1280
BslI CCNNNNNNNGG 3 cut(s) 609, 652, 1066
BsmAI GTCTC 2 cut(s) 35, 170
BsmBI CGTCTC 1 cut(s) 170
BsnI GGCC 4 cut(s) 337, 674, 877, 958
Bsp120I GGGCCC 1 cut(s) 875
Bsp1286I GDGCHC 4 cut(s) 9, 161, 303, 879
Bsp143I GATC 5 cut(s) 134, 364, 562, 1093, 1231
BspACI CCGC 8 cut(s) 17, 162, 193, 207, 590, 620, 806, 1169
BspANI GGCC 4 cut(s) 337, 674, 877, 958
BspCNI CTCAG 2 cut(s) 21, 126
BspDI ATCGAT 1 cut(s) 137
BspFNI CGCG 2 cut(s) 193, 1169
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 7 cut(s) 110, 396, 730, 867, 877, 878, 1095
BspPI GGATC 3 cut(s) 1088, 1101, 1226
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 918
BsrFI RCCGGY 1 cut(s) 24
BsrI ACTGG 2 cut(s) 630, 1221
BssAI RCCGGY 1 cut(s) 24
BssECI CCNNGG 2 cut(s) 95, 1089
BssMI GATC 5 cut(s) 134, 364, 562, 1093, 1231
BssNAI GTATAC 1 cut(s) 1165
Bst1107I GTATAC 1 cut(s) 1165
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 7 cut(s) 46, 71, 218, 712, 905, 1061, 1189
Bst6I CTCTTC 2 cut(s) 416, 1264
BstC8I GCNNGC 3 cut(s) 212, 358, 487
BstDEI CTNAG 3 cut(s) 8, 113, 406
BstF5I GGATG 2 cut(s) 270, 772
BstFNI CGCG 2 cut(s) 193, 1169
BstH2I RGCGCY 1 cut(s) 936
BstHHI GCGC 2 cut(s) 935, 1126
BstKTI GATC 5 cut(s) 137, 367, 565, 1096, 1234
BstMAI GTCTC 2 cut(s) 35, 170
BstMBI GATC 5 cut(s) 134, 364, 562, 1093, 1231
BstMWI GCNNNNNNNGC 3 cut(s) 491, 626, 803
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 1 cut(s) 879
BstUI CGCG 2 cut(s) 193, 1169
BstV1I GCAGC 3 cut(s) 267, 340, 1088
BstV2I GAAGAC 3 cut(s) 107, 255, 1183
BstX2I RGATCY 3 cut(s) 364, 1093, 1231
BstYI RGATCY 3 cut(s) 364, 1093, 1231
BstZ17I GTATAC 1 cut(s) 1165
Bsu15I ATCGAT 1 cut(s) 137
Bsu36I CCTNAGG 1 cut(s) 113
BsuI GTATCC 1 cut(s) 552
BsuRI GGCC 4 cut(s) 337, 674, 877, 958
BsuTUI ATCGAT 1 cut(s) 137
BtsCI GGATG 2 cut(s) 270, 772
Cac8I GCNNGC 3 cut(s) 212, 358, 487
CciI TCATGA 1 cut(s) 225
CfoI GCGC 2 cut(s) 935, 1126
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 7 cut(s) 117, 336, 672, 875, 876, 995, 1268
ClaI ATCGAT 1 cut(s) 137
Csp6I GTAC 2 cut(s) 122, 1148
CspAI ACCGGT 1 cut(s) 24
CviAII CATG 3 cut(s) 226, 486, 665
CviQI GTAC 2 cut(s) 122, 1148
DdeI CTNAG 3 cut(s) 8, 113, 406
DpnI GATC 5 cut(s) 136, 366, 564, 1095, 1233
DpnII GATC 5 cut(s) 134, 364, 562, 1093, 1231
Eam1104I CTCTTC 2 cut(s) 416, 1264
EarI CTCTTC 2 cut(s) 416, 1264
EciI GGCGGA 2 cut(s) 151, 635
Ecl136II GAGCTC 1 cut(s) 7
Eco24I GRGCYC 2 cut(s) 9, 879
Eco47I GGWCC 3 cut(s) 117, 995, 1268
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 2 cut(s) 440, 1221
Eco81I CCTNAGG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 876
EcoRII CCWGG 1 cut(s) 104
EcoT38I GRGCYC 2 cut(s) 9, 879
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 3 cut(s) 229, 489, 668
FalI AAGNNNNNCTT 2 cut(s) 844, 876
FatI CATG 3 cut(s) 225, 485, 664
FauI CCCGC 1 cut(s) 1162
FauNDI CATATG 1 cut(s) 751
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 4 cut(s) 208, 281, 354, 1077
FokI GGATG 2 cut(s) 277, 779
FriOI GRGCYC 2 cut(s) 9, 879
Fsp4HI GCNGC 4 cut(s) 208, 281, 354, 1077
FspBI CTAG 1 cut(s) 944
FspI TGCGCA 1 cut(s) 1125
GlaI GCGC 2 cut(s) 934, 1125
GluI GCNGC 4 cut(s) 208, 281, 354, 1077
GsuI CTGGAG 1 cut(s) 151
HaeII RGCGCY 1 cut(s) 936
HaeIII GGCC 4 cut(s) 337, 674, 877, 958
HapII CCGG 5 cut(s) 25, 145, 334, 398, 1280
HhaI GCGC 2 cut(s) 935, 1126
Hin1II CATG 3 cut(s) 229, 489, 668
Hin6I GCGC 2 cut(s) 933, 1124
HinP1I GCGC 2 cut(s) 933, 1124
HincII GTYRAC 1 cut(s) 918
HindII GTYRAC 1 cut(s) 918
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 5 cut(s) 139, 401, 415, 596, 974
HpaII CCGG 5 cut(s) 25, 145, 334, 398, 1280
HphI GGTGA 3 cut(s) 59, 389, 591
Hpy166II GTNNAC 3 cut(s) 918, 995, 1165
Hpy188I TCNGA 8 cut(s) 99, 134, 175, 420, 504, 616, 1231, 1240
Hpy188III TCNNGA 2 cut(s) 226, 368
Hpy8I GTNNAC 3 cut(s) 918, 995, 1165
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 6 cut(s) 135, 190, 324, 671, 781, 1245
HpyCH4III ACNGT 7 cut(s) 46, 71, 218, 712, 905, 1061, 1189
HpyCH4IV ACGT 2 cut(s) 179, 348
HpyCH4V TGCA 9 cut(s) 30, 268, 291, 356, 386, 668, 826, 832, 1246
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 626, 803
HpyF3I CTNAG 3 cut(s) 8, 113, 406
HpySE526I ACGT 2 cut(s) 179, 348
Hsp92II CATG 3 cut(s) 229, 489, 668
HspAI GCGC 2 cut(s) 933, 1124
Kzo9I GATC 5 cut(s) 134, 364, 562, 1093, 1231
LmnI GCTCC 5 cut(s) 4, 114, 164, 176, 336
Lsp1109I GCAGC 3 cut(s) 267, 340, 1088
LweI GCATC 3 cut(s) 255, 757, 1233
MaeI CTAG 1 cut(s) 944
MaeII ACGT 2 cut(s) 179, 348
MaeIII GTNAC 2 cut(s) 412, 597
MalI GATC 5 cut(s) 136, 366, 564, 1095, 1233
MboI GATC 5 cut(s) 134, 364, 562, 1093, 1231
MboII GAAGA 8 cut(s) 112, 260, 374, 433, 526, 1055, 1183, 1251
MfeI CAATTG 1 cut(s) 479
MflI RGATCY 3 cut(s) 364, 1093, 1231
MhlI GDGCHC 4 cut(s) 9, 161, 303, 879
MluCI AATT 8 cut(s) 87, 452, 470, 479, 681, 1006, 1048, 1272
MlyI GAGTC 3 cut(s) 409, 605, 983
MseI TTAA 3 cut(s) 458, 684, 1288
MspI CCGG 5 cut(s) 25, 145, 334, 398, 1280
MspR9I CCNGG 1 cut(s) 106
MunI CAATTG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 2 cut(s) 193, 1169
MwoI GCNNNNNNNGC 3 cut(s) 491, 626, 803
NdeI CATATG 1 cut(s) 751
NdeII GATC 5 cut(s) 134, 364, 562, 1093, 1231
NlaIII CATG 3 cut(s) 229, 489, 668
NlaIV GGNNCC 7 cut(s) 110, 396, 730, 867, 877, 878, 1095
NmuCI GTSAC 2 cut(s) 412, 597
NsbI TGCGCA 1 cut(s) 1125
NspI RCATGY 1 cut(s) 489
PaeI GCATGC 1 cut(s) 489
PagI TCATGA 1 cut(s) 225
PfeI GAWTC 2 cut(s) 139, 401
PflFI GACNNNGTC 1 cut(s) 710
PinAI ACCGGT 1 cut(s) 24
PkrI GCNGC 4 cut(s) 209, 282, 355, 1078
PleI GAGTC 3 cut(s) 409, 604, 982
PpsI GAGTC 3 cut(s) 409, 604, 982
PsiI TTATAA 1 cut(s) 1022
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 7 cut(s) 110, 396, 730, 867, 877, 878, 1095
PspOMI GGGCCC 1 cut(s) 875
PspPI GGNCC 7 cut(s) 117, 336, 672, 875, 876, 995, 1268
PsuI RGATCY 3 cut(s) 364, 1093, 1231
PsyI GACNNNGTC 1 cut(s) 710
RsaI GTAC 2 cut(s) 123, 1149
RsaNI GTAC 2 cut(s) 122, 1148
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 3 cut(s) 458, 684, 1288
SatI GCNGC 4 cut(s) 208, 281, 354, 1077
Sau3AI GATC 5 cut(s) 134, 364, 562, 1093, 1231
Sau96I GGNCC 7 cut(s) 117, 336, 672, 875, 876, 995, 1268
SchI GAGTC 3 cut(s) 409, 605, 983
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 4 cut(s) 9, 161, 303, 879
SfaNI GCATC 3 cut(s) 255, 757, 1233
SfcI CTRYAG 1 cut(s) 904
SgrAI CRCCGGYG 1 cut(s) 24
SinI GGWCC 3 cut(s) 117, 995, 1268
SmlI CTYRAG 3 cut(s) 302, 368, 1263
SmoI CTYRAG 3 cut(s) 302, 368, 1263
SpeI ACTAGT 1 cut(s) 943
SphI GCATGC 1 cut(s) 489
Sse9I AATT 8 cut(s) 87, 452, 470, 479, 681, 1006, 1048, 1272
SsiI CCGC 8 cut(s) 17, 162, 193, 207, 590, 620, 806, 1169
SspMI CTAG 1 cut(s) 944
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 104
TaaI ACNGT 7 cut(s) 46, 71, 218, 712, 905, 1061, 1189
TaiI ACGT 2 cut(s) 182, 351
TaqI TCGA 3 cut(s) 137, 951, 1194
TasI AATT 8 cut(s) 87, 452, 470, 479, 681, 1006, 1048, 1272
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 2 cut(s) 139, 401
Tru1I TTAA 3 cut(s) 458, 684, 1288
Tru9I TTAA 3 cut(s) 458, 684, 1288
TseFI GTSAC 2 cut(s) 412, 597
TseI GCWGC 3 cut(s) 280, 353, 1076
Tsp45I GTSAC 2 cut(s) 412, 597
TspDTI ATGAA 3 cut(s) 332, 653, 1056
TspGWI ACGGA 2 cut(s) 109, 779
Tth111I GACNNNGTC 1 cut(s) 710
VpaK11BI GGWCC 3 cut(s) 117, 995, 1268
XceI RCATGY 1 cut(s) 489
XmiI GTMKAC 1 cut(s) 1164
XspI CTAG 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.