Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007871

Physical Location & Seq

Genomic Coordinates Forward (+)

4706891 .. 4709163

Gene Structure 2273 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G14600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 957 bp

ATGTTACCAACTGAGTCAAAGAAAGCCTTTTCATTCAAAAGGTGTATTGAAGATGGGGACTTAGTTATTGTTTACGAAAGACATGATGTGATGAAACCAGTTAAAGTAAGTAAAGACGGTGTGCTTCAGAACCGTTTTGGTGTGTATAAGCACTCGGATTGGATTGGGAAACCATTAGGGACGAAAGTATTCAGCAACAAGGGTAAATTTGTGTACCTGTTGGCTCCTACTCCTGAACTATGGACATTGGTTTTAAGCCACAGGACTCAAATTCTTTACATTGCGGATATTAGCTTTGTGGTTATGTATCTAGAAGTCGTGCCTGGCTGTGTTGTTCTTGAGTCAGGCACAGGTAGTGGTTCGCTTTCGACCTCACTTGCTCGAGCTGTGGCTCCAACTGGACACGTTTATTCATTTGATTTCCATGAGCAGCGAGCTGTCTCTGCAAGGGAAGATTTTGAGAAGACGGGCATAAGTAGTTTAGTAACCGTAGAGGTTAGAGACATTCAAGGAGAGGGTTTTCCTGAGAAACTGTCAGGTTTGGCTGATTCTGTGTTTCTAGACCTTCCACAGCCTTGGCTTGCGGTTCCTTCTGCAGCAAAGATGTTAAAGCAGGATGGAGTTCTATGCTCTTTCTCACCTTGCATCGAGCAAGTACAACGGACTTGTGAAGTCCTCAGATCAGATTTCATTGAGATAAGAACGTTTGAGGTGCTTCTCAGAACATATGAAGTGAAGGAAGTGAAAATGGATACCAGTAGCATGGTTGGTGAGAGCCACGAGGAGGACAATGAAGGAATGAGGCCTTGCAAGAGAAAGCACCGTTCAAACGAAAACGACACTGTTTCACAGGACAACTCTTCTAATGCTTCTTCAGTCGTCATGGCTCGGCCTTGTAGTGAAGCAAGAGGTCACACCGGTTACTTAACGTTTGCAAGACTCCAATGTCTAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008174 GO:0008757 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016740 GO:0016741 GO:0031515 GO:0032259 GO:0032991 GO:0034641 GO:0034708 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043527 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0061953 GO:0071704 GO:0080009 GO:0090304 GO:0140098 GO:1901360 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GCD14

Protein Analysis

318

Amino Acids

35.44

Weight (kDa)

6.1

Isoelectric Point (pI)

40.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCD14	PF08704	69 - 313	2.1e-116	tRNA methyltransferase complex GCD14 subunit
PCMT	PF01135	97 - 165	7.5e-06	Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_31	PF13847	108 - 227	2.7e-09	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14600
fragaria_vesca	FvH4_6g51011 FvH4_6g51011 FvH4_6g51011
malus_domestica	MD09G1029500.v1.1 MD17G1031500.v1.1
prunus_persica	Prupe.3G287900_v2.0.a1
pyrus_communis	pycom111g02350 pycom17g02900
rosa_chinensis	RchiOBHm_Chr2g0172551
rosa_laevigata	RLG00000002839 RLG00000016014 RLG00000021671 RLG00000022115
rosa_multiflora	Rmu_sc0000912.1_g000006
rosa_roxburghii	Rroxscaffold_2G00079770
rosa_rugosa	Rorug02G0559800
rosa_samantha	Rh2AG635900 Rh2BG649300 Rh2CG616100 Rh2DG663700 Rh3AG202500 Rh3DG228000
rosa_wichuraiana	Rw2G052610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	945
AciI	CCGC	2 cut(s)	284, 584
AclI	AACGTT	2 cut(s)	704, 929
AcsI	RAATTY	2 cut(s)	206, 270
AcuI	CTGAAG	2 cut(s)	110, 858
AfaI	GTAC	2 cut(s)	215, 657
AfiI	CCNNNNNNNGG	1 cut(s)	784
AflIII	ACRYGT	1 cut(s)	403
AgeI	ACCGGT	1 cut(s)	917
AgsI	TTSAA	4 cut(s)	37, 50, 509, 828
AjnI	CCWGG	1 cut(s)	322
AluBI	AGCT	4 cut(s)	294, 386, 437, 953
AluI	AGCT	4 cut(s)	294, 386, 437, 953
Alw26I	GTCTC	2 cut(s)	445, 495
Ama87I	CYCGRG	1 cut(s)	381
AoxI	GGCC	2 cut(s)	803, 890
ApeKI	GCWGC	2 cut(s)	430, 596
ApoI	RAATTY	2 cut(s)	206, 270
AsiGI	ACCGGT	1 cut(s)	917
Asp700I	GAANNNNTTC	1 cut(s)	188
AsuHPI	GGTGA	2 cut(s)	630, 782
AvaI	CYCGRG	1 cut(s)	381
BauI	CACGAG	1 cut(s)	779
BbsI	GAAGAC	1 cut(s)	470
BbvI	GCAGC	2 cut(s)	442, 608
BccI	CCATC	2 cut(s)	47, 611
BciT130I	CCWGG	1 cut(s)	324
BciVI	GTATCC	1 cut(s)	745
BcoDI	GTCTC	2 cut(s)	445, 495
BfaI	CTAG	3 cut(s)	311, 560, 950
BfmI	CTRYAG	1 cut(s)	594
BfuI	GTATCC	1 cut(s)	745
BisI	GCNGC	2 cut(s)	431, 597
BlsI	GCNGC	2 cut(s)	432, 598
Bme1390I	CCNGG	1 cut(s)	324
BmeT110I	CYCGRG	1 cut(s)	381
BmiI	GGNNCC	3 cut(s)	225, 393, 588
BmrFI	CCNGG	1 cut(s)	324
BmsI	GCATC	1 cut(s)	654
BpiI	GAAGAC	1 cut(s)	470
BpuEI	CTTGAG	1 cut(s)	359
BsaJI	CCNNGG	1 cut(s)	575
BsaWI	WCCGGW	1 cut(s)	917
Bsc4I	CCNNNNNNNGG	1 cut(s)	784
Bse118I	RCCGGY	1 cut(s)	917
Bse1I	ACTGG	3 cut(s)	98, 403, 756
Bse3DI	GCAATG	1 cut(s)	279
BseBI	CCWGG	1 cut(s)	324
BseDI	CCNNGG	1 cut(s)	575
BseGI	GGATG	1 cut(s)	622
BseLI	CCNNNNNNNGG	1 cut(s)	784
BseMI	GCAATG	1 cut(s)	279
BseMII	CTCAG	3 cut(s)	516, 691, 733
BseNI	ACTGG	3 cut(s)	98, 403, 756
BseRI	GAGGAG	1 cut(s)	797
BseXI	GCAGC	2 cut(s)	442, 608
BshFI	GGCC	2 cut(s)	805, 892
BshTI	ACCGGT	1 cut(s)	917
BsiHKCI	CYCGRG	1 cut(s)	381
BsiSI	CCGG	1 cut(s)	918
BslFI	GGGAC	2 cut(s)	71, 193
BslI	CCNNNNNNNGG	1 cut(s)	784
BsmAI	GTCTC	2 cut(s)	445, 495
BsmFI	GGGAC	2 cut(s)	71, 193
BsnI	GGCC	2 cut(s)	805, 892
BsoBI	CYCGRG	1 cut(s)	381
Bsp143I	GATC	1 cut(s)	680
BspACI	CCGC	2 cut(s)	284, 584
BspANI	GGCC	2 cut(s)	805, 892
BspCNI	CTCAG	4 cut(s)	4, 517, 690, 732
BspLI	GGNNCC	3 cut(s)	225, 393, 588
BspMAI	CTGCAG	1 cut(s)	598
BsrDI	GCAATG	1 cut(s)	279
BsrFI	RCCGGY	1 cut(s)	917
BsrI	ACTGG	3 cut(s)	98, 403, 756
BssAI	RCCGGY	1 cut(s)	917
BssECI	CCNNGG	1 cut(s)	575
BssMI	GATC	1 cut(s)	680
BssSI	CACGAG	1 cut(s)	779
BssT1I	CCWWGG	1 cut(s)	575
Bst2BI	CACGAG	1 cut(s)	779
Bst2UI	CCWGG	1 cut(s)	324
Bst4CI	ACNGT	6 cut(s)	119, 134, 490, 534, 824, 844
Bst6I	CTCTTC	1 cut(s)	865
BstC8I	GCNNGC	2 cut(s)	435, 582
BstDEI	CTNAG	6 cut(s)	12, 61, 525, 677, 719, 954
BstF5I	GGATG	1 cut(s)	622
BstKTI	GATC	1 cut(s)	683
BstMAI	GTCTC	2 cut(s)	445, 495
BstMBI	GATC	1 cut(s)	680
BstMWI	GCNNNNNNNGC	1 cut(s)	443
BstNI	CCWGG	1 cut(s)	324
BstSCI	CCNGG	1 cut(s)	322
BstSFI	CTRYAG	1 cut(s)	594
BstV1I	GCAGC	2 cut(s)	442, 608
BstV2I	GAAGAC	1 cut(s)	470
BstXI	CCANNNNNNTGG	2 cut(s)	576, 763
BsuI	GTATCC	1 cut(s)	745
BsuRI	GGCC	2 cut(s)	805, 892
BtsCI	GGATG	1 cut(s)	622
BtsIMutI	CAGTG	1 cut(s)	840
Cac8I	GCNNGC	2 cut(s)	435, 582
Cfr10I	RCCGGY	1 cut(s)	917
Csp6I	GTAC	2 cut(s)	214, 656
CspAI	ACCGGT	1 cut(s)	917
CviAII	CATG	4 cut(s)	83, 425, 763, 883
CviQI	GTAC	2 cut(s)	214, 656
DdeI	CTNAG	6 cut(s)	12, 61, 525, 677, 719, 954
DpnI	GATC	1 cut(s)	682
DpnII	GATC	1 cut(s)	680
DrdI	GACNNNNNNGTC	1 cut(s)	945
DseDI	GACNNNNNNGTC	1 cut(s)	945
Eam1104I	CTCTTC	1 cut(s)	865
EarI	CTCTTC	1 cut(s)	865
Eco130I	CCWWGG	1 cut(s)	575
Eco147I	AGGCCT	1 cut(s)	805
Eco57I	CTGAAG	2 cut(s)	110, 858
Eco88I	CYCGRG	1 cut(s)	381
EcoRII	CCWGG	1 cut(s)	322
EcoT14I	CCWWGG	1 cut(s)	575
ErhI	CCWWGG	1 cut(s)	575
FaeI	CATG	4 cut(s)	86, 428, 766, 886
FalI	AAGNNNNNCTT	2 cut(s)	11, 43
FaqI	GGGAC	2 cut(s)	71, 193
FatI	CATG	4 cut(s)	82, 424, 762, 882
FauNDI	CATATG	1 cut(s)	727
Fnu4HI	GCNGC	2 cut(s)	431, 597
FokI	GGATG	1 cut(s)	629
Fsp4HI	GCNGC	2 cut(s)	431, 597
FspBI	CTAG	3 cut(s)	311, 560, 950
GluI	GCNGC	2 cut(s)	431, 597
HaeIII	GGCC	2 cut(s)	805, 892
HapII	CCGG	1 cut(s)	918
Hin1II	CATG	4 cut(s)	86, 428, 766, 886
HinfI	GANTC	5 cut(s)	14, 265, 341, 548, 939
HpaII	CCGG	1 cut(s)	918
HphI	GGTGA	2 cut(s)	630, 782
Hpy166II	GTNNAC	2 cut(s)	73, 214
Hpy188I	TCNGA	5 cut(s)	129, 157, 680, 685, 722
Hpy188III	TCNNGA	5 cut(s)	233, 311, 338, 524, 560
Hpy8I	GTNNAC	2 cut(s)	73, 214
HpyAV	CCTTC	4 cut(s)	575, 600, 730, 788
HpyCH4III	ACNGT	6 cut(s)	119, 134, 490, 534, 824, 844
HpyCH4IV	ACGT	3 cut(s)	405, 704, 929
HpyCH4V	TGCA	5 cut(s)	446, 596, 645, 810, 935
HpyF10VI	GCNNNNNNNGC	1 cut(s)	443
HpyF3I	CTNAG	6 cut(s)	12, 61, 525, 677, 719, 954
HpySE526I	ACGT	3 cut(s)	405, 704, 929
Hsp92II	CATG	4 cut(s)	86, 428, 766, 886
Kzo9I	GATC	1 cut(s)	680
LmnI	GCTCC	2 cut(s)	229, 397
Lsp1109I	GCAGC	2 cut(s)	442, 608
LweI	GCATC	1 cut(s)	654
MaeI	CTAG	3 cut(s)	311, 560, 950
MaeII	ACGT	3 cut(s)	405, 704, 929
MaeIII	GTNAC	4 cut(s)	3, 484, 911, 920
MalI	GATC	1 cut(s)	682
MboI	GATC	1 cut(s)	680
MboII	GAAGA	5 cut(s)	62, 464, 475, 852, 864
MluCI	AATT	2 cut(s)	206, 270
MlyI	GAGTC	4 cut(s)	23, 259, 350, 933
MmeI	TCCRAC	1 cut(s)	419
MnlI	CCTC	9 cut(s)	382, 487, 508, 686, 703, 775, 778, 795, 902
MroXI	GAANNNNTTC	1 cut(s)	188
MseI	TTAA	4 cut(s)	102, 254, 608, 926
MspI	CCGG	1 cut(s)	918
MspR9I	CCNGG	1 cut(s)	324
MvaI	CCWGG	1 cut(s)	324
MwoI	GCNNNNNNNGC	1 cut(s)	443
NdeI	CATATG	1 cut(s)	727
NdeII	GATC	1 cut(s)	680
NlaIII	CATG	4 cut(s)	86, 428, 766, 886
NlaIV	GGNNCC	3 cut(s)	225, 393, 588
NmeAIII	GCCGAG	1 cut(s)	868
NmuCI	GTSAC	1 cut(s)	911
PaeR7I	CTCGAG	1 cut(s)	381
PceI	AGGCCT	1 cut(s)	805
PdmI	GAANNNNTTC	1 cut(s)	188
PfeI	GAWTC	1 cut(s)	548
PinAI	ACCGGT	1 cut(s)	917
PkrI	GCNGC	2 cut(s)	432, 598
PleI	GAGTC	4 cut(s)	22, 259, 349, 933
PpsI	GAGTC	4 cut(s)	22, 259, 349, 933
Psp1406I	AACGTT	2 cut(s)	704, 929
Psp6I	CCWGG	1 cut(s)	322
PspGI	CCWGG	1 cut(s)	322
PspN4I	GGNNCC	3 cut(s)	225, 393, 588
PspXI	VCTCGAGB	1 cut(s)	381
PstI	CTGCAG	1 cut(s)	598
RsaI	GTAC	2 cut(s)	215, 657
RsaNI	GTAC	2 cut(s)	214, 656
SaqAI	TTAA	4 cut(s)	102, 254, 608, 926
SatI	GCNGC	2 cut(s)	431, 597
Sau3AI	GATC	1 cut(s)	680
SchI	GAGTC	4 cut(s)	23, 259, 350, 933
ScrFI	CCNGG	1 cut(s)	324
SfaNI	GCATC	1 cut(s)	654
SfcI	CTRYAG	1 cut(s)	594
Sfr274I	CTCGAG	1 cut(s)	381
SlaI	CTCGAG	1 cut(s)	381
SmlI	CTYRAG	2 cut(s)	338, 381
SmoI	CTYRAG	2 cut(s)	338, 381
Sse9I	AATT	2 cut(s)	206, 270
SseBI	AGGCCT	1 cut(s)	805
SsiI	CCGC	2 cut(s)	284, 584
SspMI	CTAG	3 cut(s)	311, 560, 950
StuI	AGGCCT	1 cut(s)	805
StyD4I	CCNGG	1 cut(s)	322
StyI	CCWWGG	1 cut(s)	575
TaaI	ACNGT	6 cut(s)	119, 134, 490, 534, 824, 844
TaiI	ACGT	3 cut(s)	408, 707, 932
TaqI	TCGA	3 cut(s)	368, 382, 648
TasI	AATT	2 cut(s)	206, 270
TatI	WGTACW	1 cut(s)	655
TfiI	GAWTC	1 cut(s)	548
Tru1I	TTAA	4 cut(s)	102, 254, 608, 926
Tru9I	TTAA	4 cut(s)	102, 254, 608, 926
TscAI	CASTG	1 cut(s)	847
TseFI	GTSAC	1 cut(s)	911
TseI	GCWGC	2 cut(s)	430, 596
Tsp45I	GTSAC	1 cut(s)	911
TspDTI	ATGAA	6 cut(s)	21, 107, 402, 679, 744, 807
TspGWI	ACGGA	1 cut(s)	676
TspRI	CASTG	1 cut(s)	847
XapI	RAATTY	2 cut(s)	206, 270
XbaI	TCTAGA	2 cut(s)	310, 559
XhoI	CTCGAG	1 cut(s)	381
XmnI	GAANNNNTTC	1 cut(s)	188
XspI	CTAG	3 cut(s)	311, 560, 950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.