Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0007871

Physical Location & Seq

Genomic Coordinates Forward (+)

1816668 .. 1818800

Gene Structure 2133 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1029500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 846 bp

ATGAAAGCTGTCAAAGTGTGTGCCGGTTCTGTTCTCGAAAACCGGTTCGGTTTGTTTAAGCATTCGGACTGGATAGGGAAGCAGTTTGGTTCCAAGGTTTTCAGCAGCAAGGGCGGATTTGTTTACCTGTTGGCTCCGACACCCGAGCTTTGGACTTTGGTTTTAAGCCACAGGACTCAGATTCTGTACATTGCGGACATTAGCTTTGTGATCATGTTCTTGGAGTTGGTTCCGGGATGTTTGGTTCTCGAGTCGGGGACTGGGAGTGGATCTTTGACTACTTCACTTGCAAGGGCTGTTGCTCCTAGAGGACATGTCTATACGTTTGATTTCCATGAACAACGCGCGGTCTCTGCTAGGGAGGATTTTGAGAAGACGGGATTAAGCAACGTGATCACTGTAGGAGTTAGAGATATTCAGGGTGAAGGGTTTCCGGATGATTTTTCTGGAAGGGCAGATTCTGTCTTCCTGGACCTACCCCAACCCTGGCTGGCCATCCCTTCAGCTGCGAAAATGTTGAAGCAAGATGGGGTATTATGCTCCTTCTCCCCGTGTATTGAGCAGGTGCAACGAGCATCAGAGACTATGAGGTCAAGCTTTACGGATATAAGGACGTTTGAGATTCTCCTTCGCACATATGAAGTTCGAGAATGGAAATTGGATGACTTCCAGGGAGATGAAGCTGGTTCCGTTGGATCTCATCCACGTAAAAGGCGGCAGCGATCAAGTGAAGGAAGCAATGCGCAGGAAACTACAGGATCTCCCACGGTCATGGCCAGGCCGTGCAGTGAGACCAGAGGCCACACCGGCTATTTGACATTTGCAAGACTCAAATGCCTCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008174 GO:0008757 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016740 GO:0016741 GO:0031515 GO:0032259 GO:0032991 GO:0034641 GO:0034708 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043527 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0061953 GO:0071704 GO:0080009 GO:0090304 GO:0140098 GO:1901360 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GCD14

Protein Analysis

282

Amino Acids

31.07

Weight (kDa)

7.64

Isoelectric Point (pI)

35.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCD14	PF08704	39 - 276	1.9e-99	tRNA methyltransferase complex GCD14 subunit
PCMT	PF01135	68 - 134	1.5e-06	Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_31	PF13847	78 - 200	7.2e-09	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14600
fragaria_vesca	FvH4_6g51011 FvH4_6g51011 FvH4_6g51011
malus_domestica	MD09G1029500.v1.1 MD17G1031500.v1.1
prunus_persica	Prupe.3G287900_v2.0.a1
pyrus_communis	pycom111g02350 pycom17g02900
rosa_chinensis	RchiOBHm_Chr2g0172551
rosa_laevigata	RLG00000002839 RLG00000016014 RLG00000021671 RLG00000022115
rosa_multiflora	Rmu_sc0000912.1_g000006
rosa_roxburghii	Rroxscaffold_2G00079770
rosa_rugosa	Rorug02G0559800
rosa_samantha	Rh2AG635900 Rh2BG649300 Rh2CG616100 Rh2DG663700 Rh3AG202500 Rh3DG228000
rosa_wichuraiana	Rw2G052610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	553
AasI	GACNNNNNNGTC	1 cut(s)	589
Acc16I	TGCGCA	1 cut(s)	744
Acc36I	ACCTGC	1 cut(s)	553
AccII	CGCG	2 cut(s)	345, 347
AccIII	TCCGGA	1 cut(s)	433
AciI	CCGC	4 cut(s)	114, 194, 347, 715
AclWI	GGATC	3 cut(s)	277, 703, 766
AcoI	YGGCCR	2 cut(s)	492, 774
AcuI	CTGAAG	1 cut(s)	486
AfaI	GTAC	1 cut(s)	188
AfiI	CCNNNNNNNGG	2 cut(s)	150, 486
AflIII	ACRYGT	1 cut(s)	313
AgeI	ACCGGT	1 cut(s)	42
AgsI	TTSAA	1 cut(s)	520
AjnI	CCWGG	4 cut(s)	468, 485, 669, 776
AloI	GAACNNNNNNTCC	2 cut(s)	228, 260
AluBI	AGCT	6 cut(s)	8, 148, 204, 506, 597, 683
AluI	AGCT	6 cut(s)	8, 148, 204, 506, 597, 683
Alw26I	GTCTC	3 cut(s)	355, 575, 785
AlwI	GGATC	3 cut(s)	277, 703, 766
AlwNI	CAGNNNCTG	2 cut(s)	184, 461
Ama87I	CYCGRG	2 cut(s)	143, 248
Aor13HI	TCCGGA	1 cut(s)	433
AoxI	GGCC	4 cut(s)	492, 774, 779, 799
ApeKI	GCWGC	3 cut(s)	105, 506, 718
ArsI	GACNNNNNNTTYG	2 cut(s)	188, 220
AsiGI	ACCGGT	1 cut(s)	42
Asp700I	GAANNNNTTC	1 cut(s)	429
AspLEI	GCGC	2 cut(s)	347, 745
AspS9I	GGNCC	1 cut(s)	472
AsuC2I	CCSGG	1 cut(s)	234
AsuHPI	GGTGA	1 cut(s)	434
AvaI	CYCGRG	2 cut(s)	143, 248
AvaII	GGWCC	1 cut(s)	472
BalI	TGGCCA	2 cut(s)	494, 776
BbsI	GAAGAC	2 cut(s)	380, 457
BbvI	GCAGC	3 cut(s)	117, 493, 730
BccI	CCATC	2 cut(s)	503, 521
BceAI	ACGGC	1 cut(s)	766
BciT130I	CCWGG	4 cut(s)	470, 487, 671, 778
BclI	TGATCA	2 cut(s)	210, 393
BcnI	CCSGG	1 cut(s)	234
BcoDI	GTCTC	3 cut(s)	355, 575, 785
BfaI	CTAG	2 cut(s)	306, 357
BfmI	CTRYAG	2 cut(s)	399, 753
BfuAI	ACCTGC	1 cut(s)	553
BglI	GCCNNNNNGGC	1 cut(s)	807
BisI	GCNGC	4 cut(s)	106, 507, 716, 719
BlsI	GCNGC	4 cut(s)	107, 508, 717, 720
Bme1390I	CCNGG	5 cut(s)	234, 470, 487, 671, 778
Bme18I	GGWCC	1 cut(s)	472
BmeT110I	CYCGRG	2 cut(s)	143, 248
BmgT120I	GGNCC	1 cut(s)	472
BmiI	GGNNCC	4 cut(s)	91, 135, 231, 688
BmrFI	CCNGG	5 cut(s)	234, 470, 487, 671, 778
BmrI	ACTGGG	1 cut(s)	270
BmsI	GCATC	1 cut(s)	584
BmuI	ACTGGG	1 cut(s)	270
BpiI	GAAGAC	2 cut(s)	380, 457
BpuMI	CCSGG	1 cut(s)	234
BsaAI	YACGTR	1 cut(s)	707
BsaI	GGTCTC	2 cut(s)	355, 785
BsaJI	CCNNGG	4 cut(s)	93, 485, 670, 765
BsaWI	WCCGGW	2 cut(s)	42, 433
BsaXI	ACNNNNNCTCC	2 cut(s)	745, 775
Bsc4I	CCNNNNNNNGG	2 cut(s)	150, 486
Bse118I	RCCGGY	3 cut(s)	23, 42, 806
Bse1I	ACTGG	2 cut(s)	74, 265
Bse3DI	GCAATG	2 cut(s)	189, 745
BseAI	TCCGGA	1 cut(s)	433
BseBI	CCWGG	4 cut(s)	470, 487, 671, 778
BseDI	CCNNGG	4 cut(s)	93, 485, 670, 765
BseGI	GGATG	5 cut(s)	242, 442, 495, 667, 700
BseLI	CCNNNNNNNGG	2 cut(s)	150, 486
BseMI	GCAATG	2 cut(s)	189, 745
BseMII	CTCAG	1 cut(s)	191
BseNI	ACTGG	2 cut(s)	74, 265
BseXI	GCAGC	3 cut(s)	117, 493, 730
BsgI	GTGCAG	1 cut(s)	805
Bsh1236I	CGCG	2 cut(s)	345, 347
BshFI	GGCC	4 cut(s)	494, 776, 781, 801
BshTI	ACCGGT	1 cut(s)	42
BsiHKCI	CYCGRG	2 cut(s)	143, 248
BsiSI	CCGG	5 cut(s)	24, 43, 233, 434, 807
BslFI	GGGAC	1 cut(s)	271
BslI	CCNNNNNNNGG	2 cut(s)	150, 486
BsmAI	GTCTC	3 cut(s)	355, 575, 785
BsmFI	GGGAC	1 cut(s)	271
BsmI	GAATGC	1 cut(s)	61
BsnI	GGCC	4 cut(s)	494, 776, 781, 801
Bso31I	GGTCTC	2 cut(s)	355, 785
BsoBI	CYCGRG	2 cut(s)	143, 248
Bsp13I	TCCGGA	1 cut(s)	433
Bsp1407I	TGTACA	1 cut(s)	186
Bsp143I	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
BspACI	CCGC	4 cut(s)	114, 194, 347, 715
BspANI	GGCC	4 cut(s)	494, 776, 781, 801
BspCNI	CTCAG	1 cut(s)	190
BspEI	TCCGGA	1 cut(s)	433
BspFNI	CGCG	2 cut(s)	345, 347
BspLI	GGNNCC	4 cut(s)	91, 135, 231, 688
BspMI	ACCTGC	1 cut(s)	553
BspPI	GGATC	3 cut(s)	277, 703, 766
BspTNI	GGTCTC	2 cut(s)	355, 785
BsrDI	GCAATG	2 cut(s)	189, 745
BsrFI	RCCGGY	3 cut(s)	23, 42, 806
BsrGI	TGTACA	1 cut(s)	186
BsrI	ACTGG	2 cut(s)	74, 265
BssAI	RCCGGY	3 cut(s)	23, 42, 806
BssECI	CCNNGG	4 cut(s)	93, 485, 670, 765
BssMI	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
BssT1I	CCWWGG	1 cut(s)	93
Bst2UI	CCWGG	4 cut(s)	470, 487, 671, 778
Bst4CI	ACNGT	2 cut(s)	400, 769
BstAUI	TGTACA	1 cut(s)	186
BstBAI	YACGTR	1 cut(s)	707
BstC8I	GCNNGC	1 cut(s)	492
BstDEI	CTNAG	1 cut(s)	177
BstDSI	CCRYGG	1 cut(s)	765
BstF5I	GGATG	5 cut(s)	242, 442, 495, 667, 700
BstFNI	CGCG	2 cut(s)	345, 347
BstHHI	GCGC	2 cut(s)	347, 745
BstKTI	GATC	6 cut(s)	213, 272, 396, 698, 725, 761
BstMAI	GTCTC	3 cut(s)	355, 575, 785
BstMBI	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
BstMWI	GCNNNNNNNGC	3 cut(s)	111, 353, 807
BstNI	CCWGG	4 cut(s)	470, 487, 671, 778
BstNSI	RCATGY	1 cut(s)	317
BstSCI	CCNGG	5 cut(s)	232, 468, 485, 669, 776
BstSFI	CTRYAG	2 cut(s)	399, 753
BstUI	CGCG	2 cut(s)	345, 347
BstV1I	GCAGC	3 cut(s)	117, 493, 730
BstV2I	GAAGAC	2 cut(s)	380, 457
BstX2I	RGATCY	3 cut(s)	269, 695, 758
BstXI	CCANNNNNNTGG	1 cut(s)	772
BstYI	RGATCY	3 cut(s)	269, 695, 758
BsuRI	GGCC	4 cut(s)	494, 776, 781, 801
BtgI	CCRYGG	1 cut(s)	765
BtsCI	GGATG	5 cut(s)	242, 442, 495, 667, 700
BtsI	GCAGTG	1 cut(s)	793
BtsIMutI	CAGTG	2 cut(s)	396, 793
BveI	ACCTGC	1 cut(s)	553
Cac8I	GCNNGC	1 cut(s)	492
CaiI	CAGNNNCTG	2 cut(s)	184, 461
CfoI	GCGC	2 cut(s)	347, 745
Cfr10I	RCCGGY	3 cut(s)	23, 42, 806
Cfr13I	GGNCC	1 cut(s)	472
Csp6I	GTAC	1 cut(s)	187
CspAI	ACCGGT	1 cut(s)	42
CviAII	CATG	4 cut(s)	214, 314, 335, 772
CviQI	GTAC	1 cut(s)	187
DdeI	CTNAG	1 cut(s)	177
DpnI	GATC	6 cut(s)	212, 271, 395, 697, 724, 760
DpnII	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
DrdI	GACNNNNNNGTC	1 cut(s)	589
DseDI	GACNNNNNNGTC	1 cut(s)	589
EaeI	YGGCCR	2 cut(s)	492, 774
EciI	GGCGGA	1 cut(s)	129
Eco130I	CCWWGG	1 cut(s)	93
Eco31I	GGTCTC	2 cut(s)	355, 785
Eco47I	GGWCC	1 cut(s)	472
Eco57I	CTGAAG	1 cut(s)	486
Eco88I	CYCGRG	2 cut(s)	143, 248
EcoRII	CCWGG	4 cut(s)	468, 485, 669, 776
EcoT14I	CCWWGG	1 cut(s)	93
ErhI	CCWWGG	1 cut(s)	93
FaeI	CATG	4 cut(s)	217, 317, 338, 775
FaqI	GGGAC	1 cut(s)	271
FatI	CATG	4 cut(s)	213, 313, 334, 771
FauNDI	CATATG	1 cut(s)	637
FbaI	TGATCA	2 cut(s)	210, 393
Fnu4HI	GCNGC	4 cut(s)	106, 507, 716, 719
FokI	GGATG	5 cut(s)	249, 449, 482, 674, 687
Fsp4HI	GCNGC	4 cut(s)	106, 507, 716, 719
FspBI	CTAG	2 cut(s)	306, 357
FspI	TGCGCA	1 cut(s)	744
GlaI	GCGC	2 cut(s)	346, 744
GluI	GCNGC	4 cut(s)	106, 507, 716, 719
HaeIII	GGCC	4 cut(s)	494, 776, 781, 801
HapII	CCGG	5 cut(s)	24, 43, 233, 434, 807
HhaI	GCGC	2 cut(s)	347, 745
Hin1II	CATG	4 cut(s)	217, 317, 338, 775
Hin6I	GCGC	2 cut(s)	345, 743
HinP1I	GCGC	2 cut(s)	345, 743
HindIII	AAGCTT	1 cut(s)	595
HinfI	GANTC	6 cut(s)	175, 181, 251, 458, 622, 828
HpaII	CCGG	5 cut(s)	24, 43, 233, 434, 807
HphI	GGTGA	1 cut(s)	434
Hpy166II	GTNNAC	1 cut(s)	124
Hpy188I	TCNGA	4 cut(s)	67, 138, 180, 580
Hpy188III	TCNNGA	5 cut(s)	35, 248, 434, 447, 647
Hpy8I	GTNNAC	1 cut(s)	124
HpyAV	CCTTC	6 cut(s)	419, 444, 510, 553, 638, 725
HpyCH4III	ACNGT	2 cut(s)	400, 769
HpyCH4IV	ACGT	4 cut(s)	323, 390, 614, 706
HpyCH4V	TGCA	4 cut(s)	290, 568, 786, 824
HpyF10VI	GCNNNNNNNGC	3 cut(s)	111, 353, 807
HpyF3I	CTNAG	1 cut(s)	177
HpySE526I	ACGT	4 cut(s)	323, 390, 614, 706
Hsp92II	CATG	4 cut(s)	217, 317, 338, 775
HspAI	GCGC	2 cut(s)	345, 743
Kpn2I	TCCGGA	1 cut(s)	433
Ksp22I	TGATCA	2 cut(s)	210, 393
Kzo9I	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
LmnI	GCTCC	3 cut(s)	139, 307, 545
Lsp1109I	GCAGC	3 cut(s)	117, 493, 730
LweI	GCATC	1 cut(s)	584
MaeI	CTAG	2 cut(s)	306, 357
MaeII	ACGT	4 cut(s)	323, 390, 614, 706
MalI	GATC	6 cut(s)	212, 271, 395, 697, 724, 760
MboI	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
MboII	GAAGA	2 cut(s)	385, 457
MflI	RGATCY	3 cut(s)	269, 695, 758
MlsI	TGGCCA	2 cut(s)	494, 776
MluCI	AATT	1 cut(s)	656
MluNI	TGGCCA	2 cut(s)	494, 776
MlyI	GAGTC	3 cut(s)	169, 260, 822
MmeI	TCCRAC	2 cut(s)	161, 673
MnlI	CCTC	4 cut(s)	302, 355, 582, 791
Mox20I	TGGCCA	2 cut(s)	494, 776
MroI	TCCGGA	1 cut(s)	433
MroXI	GAANNNNTTC	1 cut(s)	429
MscI	TGGCCA	2 cut(s)	494, 776
MseI	TTAA	3 cut(s)	57, 164, 383
MslI	CAYNNNNRTG	1 cut(s)	770
Msp20I	TGGCCA	2 cut(s)	494, 776
MspA1I	CMGCKG	1 cut(s)	506
MspI	CCGG	5 cut(s)	24, 43, 233, 434, 807
MspR9I	CCNGG	5 cut(s)	234, 470, 487, 671, 778
Mva1269I	GAATGC	1 cut(s)	61
MvaI	CCWGG	4 cut(s)	470, 487, 671, 778
MvnI	CGCG	2 cut(s)	345, 347
MwoI	GCNNNNNNNGC	3 cut(s)	111, 353, 807
NciI	CCSGG	1 cut(s)	234
NdeI	CATATG	1 cut(s)	637
NdeII	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
NlaIII	CATG	4 cut(s)	217, 317, 338, 775
NlaIV	GGNNCC	4 cut(s)	91, 135, 231, 688
NsbI	TGCGCA	1 cut(s)	744
NspI	RCATGY	1 cut(s)	317
PaeR7I	CTCGAG	1 cut(s)	248
PaqCI	CACCTGC	1 cut(s)	553
PciI	ACATGT	1 cut(s)	313
PctI	GAATGC	1 cut(s)	61
PdmI	GAANNNNTTC	1 cut(s)	429
PfeI	GAWTC	3 cut(s)	181, 458, 622
PfoI	TCCNGGA	2 cut(s)	232, 468
PinAI	ACCGGT	1 cut(s)	42
PkrI	GCNGC	4 cut(s)	107, 508, 717, 720
PleI	GAGTC	3 cut(s)	169, 259, 822
PpsI	GAGTC	3 cut(s)	169, 259, 822
Ppu21I	YACGTR	1 cut(s)	707
PscI	ACATGT	1 cut(s)	313
Psp6I	CCWGG	4 cut(s)	468, 485, 669, 776
PspGI	CCWGG	4 cut(s)	468, 485, 669, 776
PspN4I	GGNNCC	4 cut(s)	91, 135, 231, 688
PspPI	GGNCC	1 cut(s)	472
PstNI	CAGNNNCTG	2 cut(s)	184, 461
PsuI	RGATCY	3 cut(s)	269, 695, 758
PvuII	CAGCTG	1 cut(s)	506
RsaI	GTAC	1 cut(s)	188
RsaNI	GTAC	1 cut(s)	187
RseI	CAYNNNNRTG	1 cut(s)	770
SaqAI	TTAA	3 cut(s)	57, 164, 383
SatI	GCNGC	4 cut(s)	106, 507, 716, 719
Sau3AI	GATC	6 cut(s)	210, 269, 393, 695, 722, 758
Sau96I	GGNCC	1 cut(s)	472
SchI	GAGTC	3 cut(s)	169, 260, 822
ScrFI	CCNGG	5 cut(s)	234, 470, 487, 671, 778
SfaNI	GCATC	1 cut(s)	584
SfcI	CTRYAG	2 cut(s)	399, 753
Sfr274I	CTCGAG	1 cut(s)	248
SinI	GGWCC	1 cut(s)	472
SlaI	CTCGAG	1 cut(s)	248
SmiMI	CAYNNNNRTG	1 cut(s)	770
SmlI	CTYRAG	1 cut(s)	248
SmoI	CTYRAG	1 cut(s)	248
Sse9I	AATT	1 cut(s)	656
SsiI	CCGC	4 cut(s)	114, 194, 347, 715
SspMI	CTAG	2 cut(s)	306, 357
StyD4I	CCNGG	5 cut(s)	232, 468, 485, 669, 776
StyI	CCWWGG	1 cut(s)	93
TaaI	ACNGT	2 cut(s)	400, 769
TaiI	ACGT	4 cut(s)	326, 393, 617, 709
TaqI	TCGA	3 cut(s)	36, 249, 646
TasI	AATT	1 cut(s)	656
TatI	WGTACW	1 cut(s)	186
TauI	GCSGC	1 cut(s)	718
TfiI	GAWTC	3 cut(s)	181, 458, 622
Tru1I	TTAA	3 cut(s)	57, 164, 383
Tru9I	TTAA	3 cut(s)	57, 164, 383
TscAI	CASTG	2 cut(s)	403, 793
TseI	GCWGC	3 cut(s)	105, 506, 718
TspDTI	ATGAA	4 cut(s)	17, 351, 654, 693
TspGWI	ACGGA	2 cut(s)	617, 679
TspRI	CASTG	2 cut(s)	403, 793
VpaK11BI	GGWCC	1 cut(s)	472
XceI	RCATGY	1 cut(s)	317
XhoI	CTCGAG	1 cut(s)	248
XmnI	GAANNNNTTC	1 cut(s)	429
XspI	CTAG	2 cut(s)	306, 357

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.