Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007871

Physical Location & Seq

Genomic Coordinates Forward (+)

2282382 .. 2284457

Gene Structure 2076 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1031500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 672 bp

ATGAAAGCTGTCGAAGTGTGTGCCGGTTCTGTGCTCGGAAACCGGTTCGGCGTGTTTAAGCATTCAGACTGGATAGGGAAGCAGTTTGGTTCCAAGGTTTTCAGCAGCAAGGGAGGATTTGTTTACTTGTTAGCTCCGACACCCGAGCTTTGGACTTTGGTTTTAAGCCACAGGACTCAGATTCTGTACATTGCAGACATTAGCTTTGTGATCATGTTCTTGGAATTGGTTCCGGGATGTTTGGTTCTCGAGTCGGGGACCGGGAGTGGATCTTTGACTACTTCGCTTGCAAGGGCTGTTGCTCCTACAGGACATGTCTATACGTTTGATTTCCATGAACAACGCGCGGTCTCTGCTAGGGAGGATTTTGAGAAGACAGGATTAAGCAACTTGGTCACTGTACGAGTTAGAGATATTCAGGGAGAAGGATTTCCGGATGAGTTTTCTGGAAGGGCAGATTCTGTCTTCCTGGACTTACCCCAACCTTGGCTGGCTATCCCTTCAGCTGCGAAAATGTTGAAACAAGATGGGGTACTATGCTCCTTCTCCCCGTGTATTGAGCAGGTGCAACGAGCGTCAGAGACTATGAGATCAAGCTTTACGGATATAAGGACGTTTGAGATACTCCTTCACACATATGAAGTTCGAGAATGGAAATTGGATGACTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008174 GO:0008757 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016740 GO:0016741 GO:0031515 GO:0032259 GO:0032991 GO:0034641 GO:0034708 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043527 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0061953 GO:0071704 GO:0080009 GO:0090304 GO:0140098 GO:1901360 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GCD14

Protein Analysis

224

Amino Acids

24.81

Weight (kDa)

5.55

Isoelectric Point (pI)

29.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCD14	PF08704	39 - 221	3.8e-89	tRNA methyltransferase complex GCD14 subunit
PCMT	PF01135	68 - 134	2e-06	Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_31	PF13847	78 - 200	9e-10	Methyltransferase domain
Methyltransf_25	PF13649	82 - 177	3.7e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14600
fragaria_vesca	FvH4_6g51011 FvH4_6g51011 FvH4_6g51011
malus_domestica	MD09G1029500.v1.1 MD17G1031500.v1.1
prunus_persica	Prupe.3G287900_v2.0.a1
pyrus_communis	pycom111g02350 pycom17g02900
rosa_chinensis	RchiOBHm_Chr2g0172551
rosa_laevigata	RLG00000002839 RLG00000016014 RLG00000021671 RLG00000022115
rosa_multiflora	Rmu_sc0000912.1_g000006
rosa_roxburghii	Rroxscaffold_2G00079770
rosa_rugosa	Rorug02G0559800
rosa_samantha	Rh2AG635900 Rh2BG649300 Rh2CG616100 Rh2DG663700 Rh3AG202500 Rh3DG228000
rosa_wichuraiana	Rw2G052610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	553
Acc36I	ACCTGC	1 cut(s)	553
AccII	CGCG	2 cut(s)	345, 347
AccIII	TCCGGA	1 cut(s)	433
AciI	CCGC	1 cut(s)	347
AclWI	GGATC	1 cut(s)	277
AcuI	CTGAAG	1 cut(s)	486
AfaI	GTAC	3 cut(s)	188, 402, 534
AfiI	CCNNNNNNNGG	2 cut(s)	150, 486
AflIII	ACRYGT	1 cut(s)	313
AgeI	ACCGGT	1 cut(s)	42
AgsI	TTSAA	1 cut(s)	520
AjnI	CCWGG	1 cut(s)	468
AloI	GAACNNNNNNTCC	2 cut(s)	228, 260
AluBI	AGCT	6 cut(s)	8, 134, 148, 204, 506, 597
AluI	AGCT	6 cut(s)	8, 134, 148, 204, 506, 597
Alw21I	GWGCWC	1 cut(s)	36
Alw26I	GTCTC	2 cut(s)	355, 575
AlwI	GGATC	1 cut(s)	277
AlwNI	CAGNNNCTG	2 cut(s)	184, 461
Ama87I	CYCGRG	2 cut(s)	143, 248
Aor13HI	TCCGGA	1 cut(s)	433
ApeKI	GCWGC	2 cut(s)	105, 506
ArsI	GACNNNNNNTTYG	2 cut(s)	188, 220
AsiGI	ACCGGT	1 cut(s)	42
Asp700I	GAANNNNTTC	2 cut(s)	228, 429
AspLEI	GCGC	1 cut(s)	347
AspS9I	GGNCC	1 cut(s)	258
AsuC2I	CCSGG	2 cut(s)	234, 262
AvaI	CYCGRG	2 cut(s)	143, 248
AvaII	GGWCC	1 cut(s)	258
BbsI	GAAGAC	2 cut(s)	380, 457
Bbv12I	GWGCWC	1 cut(s)	36
BbvI	GCAGC	2 cut(s)	117, 493
BccI	CCATC	1 cut(s)	521
BcgI	CGANNNNNNTGC	1 cut(s)	36
BciT130I	CCWGG	1 cut(s)	470
BclI	TGATCA	1 cut(s)	210
BcnI	CCSGG	2 cut(s)	234, 262
BcoDI	GTCTC	2 cut(s)	355, 575
BfaI	CTAG	1 cut(s)	357
BfmI	CTRYAG	1 cut(s)	306
BfuAI	ACCTGC	1 cut(s)	553
BisI	GCNGC	2 cut(s)	106, 507
BlsI	GCNGC	2 cut(s)	107, 508
Bme1390I	CCNGG	3 cut(s)	234, 262, 470
Bme18I	GGWCC	1 cut(s)	258
BmeT110I	CYCGRG	2 cut(s)	143, 248
BmgT120I	GGNCC	1 cut(s)	258
BmiI	GGNNCC	3 cut(s)	91, 231, 259
BmrFI	CCNGG	3 cut(s)	234, 262, 470
BpiI	GAAGAC	2 cut(s)	380, 457
BpuMI	CCSGG	2 cut(s)	234, 262
BsaI	GGTCTC	1 cut(s)	355
BsaJI	CCNNGG	2 cut(s)	93, 485
BsaWI	WCCGGW	2 cut(s)	42, 433
BsaXI	ACNNNNNCTCC	2 cut(s)	105, 135
Bsc4I	CCNNNNNNNGG	2 cut(s)	150, 486
Bse118I	RCCGGY	2 cut(s)	23, 42
Bse1I	ACTGG	1 cut(s)	74
Bse3DI	GCAATG	1 cut(s)	189
BseAI	TCCGGA	1 cut(s)	433
BseBI	CCWGG	1 cut(s)	470
BseDI	CCNNGG	2 cut(s)	93, 485
BseGI	GGATG	3 cut(s)	242, 442, 667
BseLI	CCNNNNNNNGG	2 cut(s)	150, 486
BseMI	GCAATG	1 cut(s)	189
BseMII	CTCAG	1 cut(s)	191
BseNI	ACTGG	1 cut(s)	74
BseXI	GCAGC	2 cut(s)	117, 493
Bsh1236I	CGCG	2 cut(s)	345, 347
BshTI	ACCGGT	1 cut(s)	42
BsiHKAI	GWGCWC	1 cut(s)	36
BsiHKCI	CYCGRG	2 cut(s)	143, 248
BsiSI	CCGG	5 cut(s)	24, 43, 233, 261, 434
BslFI	GGGAC	1 cut(s)	271
BslI	CCNNNNNNNGG	2 cut(s)	150, 486
BsmAI	GTCTC	2 cut(s)	355, 575
BsmFI	GGGAC	1 cut(s)	271
BsmI	GAATGC	1 cut(s)	61
Bso31I	GGTCTC	1 cut(s)	355
BsoBI	CYCGRG	2 cut(s)	143, 248
Bsp1286I	GDGCHC	1 cut(s)	36
Bsp13I	TCCGGA	1 cut(s)	433
Bsp1407I	TGTACA	1 cut(s)	186
Bsp143I	GATC	3 cut(s)	210, 269, 590
BspACI	CCGC	1 cut(s)	347
BspCNI	CTCAG	1 cut(s)	190
BspEI	TCCGGA	1 cut(s)	433
BspFNI	CGCG	2 cut(s)	345, 347
BspLI	GGNNCC	3 cut(s)	91, 231, 259
BspMI	ACCTGC	1 cut(s)	553
BspPI	GGATC	1 cut(s)	277
BspTNI	GGTCTC	1 cut(s)	355
BsrDI	GCAATG	1 cut(s)	189
BsrFI	RCCGGY	2 cut(s)	23, 42
BsrGI	TGTACA	1 cut(s)	186
BsrI	ACTGG	1 cut(s)	74
BssAI	RCCGGY	2 cut(s)	23, 42
BssECI	CCNNGG	2 cut(s)	93, 485
BssMI	GATC	3 cut(s)	210, 269, 590
BssT1I	CCWWGG	2 cut(s)	93, 485
Bst2UI	CCWGG	1 cut(s)	470
Bst4CI	ACNGT	1 cut(s)	400
BstAUI	TGTACA	1 cut(s)	186
BstC8I	GCNNGC	2 cut(s)	288, 492
BstDEI	CTNAG	1 cut(s)	177
BstF5I	GGATG	3 cut(s)	242, 442, 667
BstFNI	CGCG	2 cut(s)	345, 347
BstHHI	GCGC	1 cut(s)	347
BstKTI	GATC	3 cut(s)	213, 272, 593
BstMAI	GTCTC	2 cut(s)	355, 575
BstMBI	GATC	3 cut(s)	210, 269, 590
BstMWI	GCNNNNNNNGC	1 cut(s)	353
BstNI	CCWGG	1 cut(s)	470
BstNSI	RCATGY	1 cut(s)	317
BstSCI	CCNGG	3 cut(s)	232, 260, 468
BstSFI	CTRYAG	1 cut(s)	306
BstUI	CGCG	2 cut(s)	345, 347
BstV1I	GCAGC	2 cut(s)	117, 493
BstV2I	GAAGAC	2 cut(s)	380, 457
BstX2I	RGATCY	1 cut(s)	269
BstYI	RGATCY	1 cut(s)	269
BtsCI	GGATG	3 cut(s)	242, 442, 667
BtsIMutI	CAGTG	1 cut(s)	396
BveI	ACCTGC	1 cut(s)	553
Cac8I	GCNNGC	2 cut(s)	288, 492
CaiI	CAGNNNCTG	2 cut(s)	184, 461
CfoI	GCGC	1 cut(s)	347
Cfr10I	RCCGGY	2 cut(s)	23, 42
Cfr13I	GGNCC	1 cut(s)	258
CseI	GACGC	1 cut(s)	564
Csp6I	GTAC	3 cut(s)	187, 401, 533
CspAI	ACCGGT	1 cut(s)	42
CviAII	CATG	3 cut(s)	214, 314, 335
CviQI	GTAC	3 cut(s)	187, 401, 533
DdeI	CTNAG	1 cut(s)	177
DpnI	GATC	3 cut(s)	212, 271, 592
DpnII	GATC	3 cut(s)	210, 269, 590
Eco130I	CCWWGG	2 cut(s)	93, 485
Eco31I	GGTCTC	1 cut(s)	355
Eco47I	GGWCC	1 cut(s)	258
Eco57I	CTGAAG	1 cut(s)	486
Eco88I	CYCGRG	2 cut(s)	143, 248
EcoRII	CCWGG	1 cut(s)	468
EcoT14I	CCWWGG	2 cut(s)	93, 485
ErhI	CCWWGG	2 cut(s)	93, 485
FaeI	CATG	3 cut(s)	217, 317, 338
FaiI	YATR	9 cut(s)	215, 315, 321, 336, 538, 587, 608, 637, 639
FaqI	GGGAC	1 cut(s)	271
FatI	CATG	3 cut(s)	213, 313, 334
FauNDI	CATATG	1 cut(s)	637
FbaI	TGATCA	1 cut(s)	210
Fnu4HI	GCNGC	2 cut(s)	106, 507
FokI	GGATG	2 cut(s)	249, 449
Fsp4HI	GCNGC	2 cut(s)	106, 507
FspBI	CTAG	1 cut(s)	357
GlaI	GCGC	1 cut(s)	346
GluI	GCNGC	2 cut(s)	106, 507
HapII	CCGG	5 cut(s)	24, 43, 233, 261, 434
HgaI	GACGC	1 cut(s)	564
HhaI	GCGC	1 cut(s)	347
Hin1II	CATG	3 cut(s)	217, 317, 338
Hin6I	GCGC	1 cut(s)	345
HinP1I	GCGC	1 cut(s)	345
HindIII	AAGCTT	1 cut(s)	595
HinfI	GANTC	4 cut(s)	175, 181, 251, 458
HpaII	CCGG	5 cut(s)	24, 43, 233, 261, 434
Hpy166II	GTNNAC	1 cut(s)	124
Hpy188I	TCNGA	5 cut(s)	38, 67, 138, 180, 580
Hpy188III	TCNNGA	4 cut(s)	248, 434, 447, 647
Hpy8I	GTNNAC	1 cut(s)	124
HpyAV	CCTTC	5 cut(s)	419, 444, 510, 553, 638
HpyCH4III	ACNGT	1 cut(s)	400
HpyCH4IV	ACGT	2 cut(s)	323, 614
HpyCH4V	TGCA	3 cut(s)	194, 290, 568
HpyF10VI	GCNNNNNNNGC	1 cut(s)	353
HpyF3I	CTNAG	1 cut(s)	177
HpySE526I	ACGT	2 cut(s)	323, 614
Hsp92II	CATG	3 cut(s)	217, 317, 338
HspAI	GCGC	1 cut(s)	345
Kpn2I	TCCGGA	1 cut(s)	433
Ksp22I	TGATCA	1 cut(s)	210
Kzo9I	GATC	3 cut(s)	210, 269, 590
LmnI	GCTCC	3 cut(s)	139, 307, 545
Lsp1109I	GCAGC	2 cut(s)	117, 493
MaeI	CTAG	1 cut(s)	357
MaeII	ACGT	2 cut(s)	323, 614
MaeIII	GTNAC	1 cut(s)	394
MalI	GATC	3 cut(s)	212, 271, 592
MboI	GATC	3 cut(s)	210, 269, 590
MboII	GAAGA	2 cut(s)	385, 457
MflI	RGATCY	1 cut(s)	269
MhlI	GDGCHC	1 cut(s)	36
MluCI	AATT	2 cut(s)	224, 656
MlyI	GAGTC	2 cut(s)	169, 260
MmeI	TCCRAC	1 cut(s)	161
MnlI	CCTC	2 cut(s)	107, 355
MroI	TCCGGA	1 cut(s)	433
MroXI	GAANNNNTTC	2 cut(s)	228, 429
MseI	TTAA	3 cut(s)	57, 164, 383
MslI	CAYNNNNRTG	1 cut(s)	636
MspA1I	CMGCKG	1 cut(s)	506
MspI	CCGG	5 cut(s)	24, 43, 233, 261, 434
MspR9I	CCNGG	3 cut(s)	234, 262, 470
Mva1269I	GAATGC	1 cut(s)	61
MvaI	CCWGG	1 cut(s)	470
MvnI	CGCG	2 cut(s)	345, 347
MwoI	GCNNNNNNNGC	1 cut(s)	353
NciI	CCSGG	2 cut(s)	234, 262
NdeI	CATATG	1 cut(s)	637
NdeII	GATC	3 cut(s)	210, 269, 590
NlaIII	CATG	3 cut(s)	217, 317, 338
NlaIV	GGNNCC	3 cut(s)	91, 231, 259
NmuCI	GTSAC	1 cut(s)	394
NspI	RCATGY	1 cut(s)	317
PaeR7I	CTCGAG	1 cut(s)	248
PaqCI	CACCTGC	1 cut(s)	553
PciI	ACATGT	1 cut(s)	313
PctI	GAATGC	1 cut(s)	61
PdmI	GAANNNNTTC	2 cut(s)	228, 429
PfeI	GAWTC	2 cut(s)	181, 458
PfoI	TCCNGGA	2 cut(s)	232, 468
PinAI	ACCGGT	1 cut(s)	42
PkrI	GCNGC	2 cut(s)	107, 508
PleI	GAGTC	2 cut(s)	169, 259
PpsI	GAGTC	2 cut(s)	169, 259
PscI	ACATGT	1 cut(s)	313
Psp6I	CCWGG	1 cut(s)	468
PspGI	CCWGG	1 cut(s)	468
PspN4I	GGNNCC	3 cut(s)	91, 231, 259
PspPI	GGNCC	1 cut(s)	258
PstNI	CAGNNNCTG	2 cut(s)	184, 461
PsuI	RGATCY	1 cut(s)	269
PvuII	CAGCTG	1 cut(s)	506
RsaI	GTAC	3 cut(s)	188, 402, 534
RsaNI	GTAC	3 cut(s)	187, 401, 533
RseI	CAYNNNNRTG	1 cut(s)	636
SaqAI	TTAA	3 cut(s)	57, 164, 383
SatI	GCNGC	2 cut(s)	106, 507
Sau3AI	GATC	3 cut(s)	210, 269, 590
Sau96I	GGNCC	1 cut(s)	258
SchI	GAGTC	2 cut(s)	169, 260
ScrFI	CCNGG	3 cut(s)	234, 262, 470
SduI	GDGCHC	1 cut(s)	36
SfcI	CTRYAG	1 cut(s)	306
Sfr274I	CTCGAG	1 cut(s)	248
SinI	GGWCC	1 cut(s)	258
SlaI	CTCGAG	1 cut(s)	248
SmiMI	CAYNNNNRTG	1 cut(s)	636
SmlI	CTYRAG	1 cut(s)	248
SmoI	CTYRAG	1 cut(s)	248
Sse9I	AATT	2 cut(s)	224, 656
SsiI	CCGC	1 cut(s)	347
SspMI	CTAG	1 cut(s)	357
StyD4I	CCNGG	3 cut(s)	232, 260, 468
StyI	CCWWGG	2 cut(s)	93, 485
TaaI	ACNGT	1 cut(s)	400
TaiI	ACGT	2 cut(s)	326, 617
TaqI	TCGA	3 cut(s)	12, 249, 646
TasI	AATT	2 cut(s)	224, 656
TatI	WGTACW	1 cut(s)	186
TfiI	GAWTC	2 cut(s)	181, 458
Tru1I	TTAA	3 cut(s)	57, 164, 383
Tru9I	TTAA	3 cut(s)	57, 164, 383
TscAI	CASTG	1 cut(s)	403
TseFI	GTSAC	1 cut(s)	394
TseI	GCWGC	2 cut(s)	105, 506
Tsp45I	GTSAC	1 cut(s)	394
TspDTI	ATGAA	3 cut(s)	17, 351, 654
TspGWI	ACGGA	1 cut(s)	617
TspRI	CASTG	1 cut(s)	403
VpaK11BI	GGWCC	1 cut(s)	258
XceI	RCATGY	1 cut(s)	317
XhoI	CTCGAG	1 cut(s)	248
XmnI	GAANNNNTTC	2 cut(s)	228, 429
XspI	CTAG	1 cut(s)	357

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.