AT5G16320

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013187
Physical Location & Seq
Forward (+)
5344495 .. 5346106
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16320.1

Sequence Viewer

Length: 1413 bp
ATGACGGCGAGTGAGACTATCGCCACCGCGATAAATCAGATTGATGAGAAGAAGGAAAAGCTAAAGAAAGCTTTCGACGATCTTCAAGCTCATCGCTCTCTCCTTTCACCGTCTTTCTCACTTTCCTGGTCTGAGATCGATTCTCACTTTTCCTCTCTCCAATCCTCCCTTGCTAGCCGTTTCCGCCTCCTCCACTCTACATCTCCTTTGGAACATGATTCGTATAGAATCGACGCTTCTGATGCAGGGAAATCGTCGTCTTCGGAGGAGGTATCGGAGCAACCCGTTGTTGAGCCGGAGTTGAGAGCCTTGTGTGAGAAGATTGACGGAATTGGTTTGATTAAGTATTTGATTAGGATATGGGATGATGAAACTCCTCTCAATCAGGAAGTTTCTGCAGCGATTCGTTACTCTCCGGATACGGCGTCCATGGTGCTTGACGCGATTGAAGGTTCGAACTACACACCGTCATCATCGGGAAGATCATTCGATGTAAGGAGAGTTTTCGTTTTGTTAATGGAAGTTTTGATTGAGATCAATGCGAATATCACTGTTGATACGAGAAATAGAGCTAAGAAACTGGCTTACCATTGGAAATCTAAGGTTGGTGTCAAGCCTTTCGAGGCGTTAGTGTTTCTGCATTTGGTCGCAGCTTTTGAATTAGGATCAGAATTTGACACAGAGGAGCTTTCTGATTACGTTTTCATGATCGCTAAGTATAAGCAAGCAACTTTGGTTTGTAACAAGATTGGTGTAGATAGGAAAAGAGTTGGGAAACTCATCAAGACTCTCTTGGATTCTGGTAAACCAATTTTAGCAGTCAAATTCATGTATGAGTGTGGGATGACTGATGAATTTGAGCCAATCCCTGTTTTGAAATCTTACATTAAGGACTGTAGAGAAGCTGCTCTTAGAGTTTGCGTGGAAGATAACTACTCTCTCAAGTCGCAGAACGAAGCCTCTGATAAAGAAGTCAGTGCCTTGAAACCATTGATCAAAATCATCAAAGATCAGAACCTTGAATCAGAGTTTACTCAGGAGAAAGTTGAGGAACGTGTAGAAGAGCTGGAGAAAAATAAGGCATTGAGGAAACGCAACACGACTAATCCTCCCAAGCAAGAGCCACAGCAAAAGGGGAAAAAGCGGACTAGAGACTGCAAAAATGGGTCACAAGTTCCAGTTCCGAGTCAGCAGCTACTTTCTAGACCAGAAGCTCTTCTTATGCCTGAGCATAGTCATCATGGCTTACAGTTGAACCCTTATGGTCTTATGACCTCAGCATTTTCTGGTGTTGTTGTGAACCCATTAACTGGGCTCTTTGGTTCAGGTGCAACTCCTCAATCTCTTTACTATGCTCAGCAAACTGGATATGTGTTGCCGCCTCAATACCACCCGCCTTATTATTCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0010228 GO:0022414 GO:0032501 GO:0032502 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

470

Amino Acids

52.83

Weight (kDa)

6.26

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 95 - 367 1.1e-87 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31814 AT5G16320
fragaria_vesca FvH4_3g24000
malus_domestica MD11G1205100.v1.1
prunus_persica Prupe.4G232500_v2.0.a1
pyrus_communis pycom11g17780
rosa_chinensis RchiOBHm_Chr5g0042571
rosa_laevigata RLG00000034141
rosa_multiflora Rmu_sc0004704.1_g000003
rosa_roxburghii Rroxscaffold_1G00038070
rosa_rugosa Rorug05G0202600 Rorug05G0202700 Rorug05G0202800
rosa_samantha Rh5AG287800 Rh5BG292200 Rh5CG324600 Rh5DG302000
rosa_wichuraiana Rw5G026720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1310
AccII CGCG 2 cut(s) 29, 443
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 5 cut(s) 27, 184, 1144, 1379, 1394
AclWI GGATC 1 cut(s) 673
AcsI RAATTY 3 cut(s) 671, 824, 854
AcyI GRCGYC 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 1310
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 7 cut(s) 86, 449, 659, 877, 985, 1022, 1255
AjnI CCWGG 1 cut(s) 125
Alw26I GTCTC 2 cut(s) 8, 1146
AlwI GGATC 1 cut(s) 673
Aor13HI TCCGGA 1 cut(s) 415
ApeKI GCWGC 4 cut(s) 398, 650, 905, 1192
ApoI RAATTY 3 cut(s) 671, 824, 854
Asp700I GAANNNNTTC 2 cut(s) 71, 1215
AsuHPI GGTGA 1 cut(s) 99
AsuII TTCGAA 1 cut(s) 455
AsuNHI GCTAGC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 1317
BbsI GAAGAC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 1276
BbvI GCAGC 4 cut(s) 410, 662, 892, 1204
BceAI ACGGC 3 cut(s) 21, 162, 438
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 1 cut(s) 127
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 993
BcoDI GTCTC 2 cut(s) 8, 1146
BfaI CTAG 3 cut(s) 174, 1149, 1203
BfmI CTRYAG 2 cut(s) 396, 895
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 5 cut(s) 399, 651, 906, 1193, 1379
BlpI GCTNAGC 1 cut(s) 1356
BlsI GCNGC 5 cut(s) 400, 652, 907, 1194, 1380
Bme1390I CCNGG 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 127
BmrI ACTGGG 1 cut(s) 1320
BmsI GCATC 1 cut(s) 232
BmtI GCTAGC 1 cut(s) 177
BmuI ACTGGG 1 cut(s) 1320
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 1088
Bpu10I CCTNAGC 2 cut(s) 1227, 1276
Bpu1102I GCTNAGC 1 cut(s) 1356
Bpu14I TTCGAA 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 926
Bsa29I ATCGAT 1 cut(s) 138
BsaBI GATNNNNATC 2 cut(s) 533, 998
BsaHI GRCGYC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 429
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 1093, 1123
Bsc4I CCNNNNNNNGG 1 cut(s) 1310
Bse1I ACTGG 4 cut(s) 585, 1178, 1315, 1369
Bse8I GATNNNNATC 2 cut(s) 533, 998
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 1 cut(s) 127
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 2 cut(s) 370, 849
BseJI GATNNNNATC 2 cut(s) 533, 998
BseLI CCNNNNNNNGG 1 cut(s) 1310
BseMII CTCAG 5 cut(s) 123, 1049, 1218, 1290, 1370
BseNI ACTGG 4 cut(s) 585, 1178, 1315, 1369
BseRI GAGGAG 5 cut(s) 179, 281, 366, 698, 1326
BseXI GCAGC 4 cut(s) 410, 662, 892, 1204
Bsh1236I CGCG 2 cut(s) 29, 443
BshVI ATCGAT 1 cut(s) 138
BsiSI CCGG 2 cut(s) 296, 416
BslI CCNNNNNNNGG 1 cut(s) 1310
BsmAI GTCTC 2 cut(s) 8, 1146
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 1 cut(s) 1317
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
Bsp1720I GCTNAGC 1 cut(s) 1356
Bsp19I CCATGG 1 cut(s) 429
BspACI CCGC 5 cut(s) 27, 184, 1144, 1379, 1394
BspCNI CTCAG 5 cut(s) 124, 1048, 1219, 1289, 1369
BspDI ATCGAT 1 cut(s) 138
BspEI TCCGGA 1 cut(s) 415
BspFNI CGCG 2 cut(s) 29, 443
BspHI TCATGA 1 cut(s) 705
BspMAI CTGCAG 1 cut(s) 400
BspOI GCTAGC 1 cut(s) 177
BspPI GGATC 1 cut(s) 673
BspQI GCTCTTC 2 cut(s) 1056, 1221
BspT104I TTCGAA 1 cut(s) 455
BsrI ACTGG 4 cut(s) 585, 1178, 1315, 1369
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 1 cut(s) 429
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 5 cut(s) 111, 468, 553, 896, 1251
Bst6I CTCTTC 2 cut(s) 1056, 1221
BstACI GRCGYC 1 cut(s) 425
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 175, 726
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 370, 849
BstFNI CGCG 2 cut(s) 29, 443
BstKTI GATC 8 cut(s) 82, 138, 485, 537, 668, 711, 996, 1012
BstMAI GTCTC 2 cut(s) 8, 1146
BstMBI GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 183, 242
BstNI CCWGG 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 125
BstSFI CTRYAG 2 cut(s) 396, 895
BstUI CGCG 2 cut(s) 29, 443
BstV1I GCAGC 4 cut(s) 410, 662, 892, 1204
BstV2I GAAGAC 1 cut(s) 252
Bsu15I ATCGAT 1 cut(s) 138
BsuI GTATCC 1 cut(s) 412
BsuTUI ATCGAT 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 77
BtsCI GGATG 2 cut(s) 370, 849
BtsIMutI CAGTG 2 cut(s) 549, 982
Cac8I GCNNGC 2 cut(s) 175, 726
CciI TCATGA 1 cut(s) 705
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 3 cut(s) 242, 414, 449
CviAII CATG 5 cut(s) 215, 430, 706, 829, 1241
DpnI GATC 8 cut(s) 81, 137, 484, 536, 667, 710, 995, 1011
DpnII GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
Eam1104I CTCTTC 2 cut(s) 1056, 1221
EarI CTCTTC 2 cut(s) 1056, 1221
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 1317
EcoRII CCWGG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 1317
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 5 cut(s) 218, 433, 709, 832, 1244
FalI AAGNNNNNCTT 6 cut(s) 776, 808, 894, 926, 1203, 1235
FatI CATG 5 cut(s) 214, 429, 705, 828, 1240
FauI CCCGC 1 cut(s) 1401
FbaI TGATCA 1 cut(s) 993
Fnu4HI GCNGC 5 cut(s) 399, 651, 906, 1193, 1379
FokI GGATG 2 cut(s) 377, 856
FriOI GRGCYC 1 cut(s) 1317
Fsp4HI GCNGC 5 cut(s) 399, 651, 906, 1193, 1379
FspBI CTAG 3 cut(s) 174, 1149, 1203
GluI GCNGC 5 cut(s) 399, 651, 906, 1193, 1379
GsuI CTGGAG 1 cut(s) 1088
HapII CCGG 2 cut(s) 296, 416
HgaI GACGC 3 cut(s) 242, 414, 449
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 5 cut(s) 218, 433, 709, 832, 1244
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 8 cut(s) 140, 218, 228, 403, 787, 797, 1022, 1186
HpaII CCGG 2 cut(s) 296, 416
HphI GGTGA 1 cut(s) 99
Hpy166II GTNNAC 3 cut(s) 806, 1032, 1300
Hpy188III TCNNGA 7 cut(s) 386, 416, 477, 706, 784, 1037, 1203
Hpy8I GTNNAC 3 cut(s) 806, 1032, 1300
Hpy99I CGWCG 3 cut(s) 80, 236, 259
HpyAV CCTTC 2 cut(s) 46, 443
HpyCH4III ACNGT 5 cut(s) 111, 468, 553, 896, 1251
HpyCH4IV ACGT 2 cut(s) 699, 1054
HpyCH4V TGCA 5 cut(s) 245, 398, 640, 1158, 1331
HpyF10VI GCNNNNNNNGC 2 cut(s) 183, 242
HpySE526I ACGT 2 cut(s) 699, 1054
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 5 cut(s) 218, 433, 709, 832, 1244
Kpn2I TCCGGA 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 993
Kzo9I GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
LguI GCTCTTC 2 cut(s) 1056, 1221
LmnI GCTCC 2 cut(s) 277, 685
Lsp1109I GCAGC 4 cut(s) 410, 662, 892, 1204
LweI GCATC 1 cut(s) 232
MaeI CTAG 3 cut(s) 174, 1149, 1203
MaeII ACGT 2 cut(s) 699, 1054
MaeIII GTNAC 3 cut(s) 407, 740, 1167
MalI GATC 8 cut(s) 81, 137, 484, 536, 667, 710, 995, 1011
MboI GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
MboII GAAGA 8 cut(s) 61, 74, 252, 331, 492, 938, 1073, 1208
MhlI GDGCHC 1 cut(s) 1317
MluCI AATT 6 cut(s) 330, 659, 671, 810, 824, 854
MlyI GAGTC 2 cut(s) 781, 1195
MroI TCCGGA 1 cut(s) 415
MroXI GAANNNNTTC 2 cut(s) 71, 1215
MseI TTAA 4 cut(s) 342, 515, 888, 1307
MspI CCGG 2 cut(s) 296, 416
MspR9I CCNGG 1 cut(s) 127
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 2 cut(s) 29, 443
MwoI GCNNNNNNNGC 2 cut(s) 183, 242
NcoI CCATGG 1 cut(s) 429
NdeII GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
NheI GCTAGC 1 cut(s) 173
NlaIII CATG 5 cut(s) 218, 433, 709, 832, 1244
NmuCI GTSAC 1 cut(s) 1167
NspV TTCGAA 1 cut(s) 455
PagI TCATGA 1 cut(s) 705
PciSI GCTCTTC 2 cut(s) 1056, 1221
PdmI GAANNNNTTC 2 cut(s) 71, 1215
PfeI GAWTC 6 cut(s) 140, 218, 228, 403, 797, 1022
PflMI CCANNNNNTGG 1 cut(s) 1310
PkrI GCNGC 5 cut(s) 400, 652, 907, 1194, 1380
PleI GAGTC 2 cut(s) 781, 1194
PpsI GAGTC 2 cut(s) 781, 1194
Psp6I CCWGG 1 cut(s) 125
PspGI CCWGG 1 cut(s) 125
PstI CTGCAG 1 cut(s) 400
SapI GCTCTTC 2 cut(s) 1056, 1221
SaqAI TTAA 4 cut(s) 342, 515, 888, 1307
SatI GCNGC 5 cut(s) 399, 651, 906, 1193, 1379
Sau3AI GATC 8 cut(s) 79, 135, 482, 534, 665, 708, 993, 1009
SchI GAGTC 2 cut(s) 781, 1195
ScrFI CCNGG 1 cut(s) 127
SduI GDGCHC 1 cut(s) 1317
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 2 cut(s) 396, 895
SfuI TTCGAA 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
Sse9I AATT 6 cut(s) 330, 659, 671, 810, 824, 854
SsiI CCGC 5 cut(s) 27, 184, 1144, 1379, 1394
SspMI CTAG 3 cut(s) 174, 1149, 1203
StyD4I CCNGG 1 cut(s) 125
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 5 cut(s) 111, 468, 553, 896, 1251
TaiI ACGT 2 cut(s) 702, 1057
TaqI TCGA 6 cut(s) 75, 138, 231, 455, 489, 621
TasI AATT 6 cut(s) 330, 659, 671, 810, 824, 854
TauI GCSGC 1 cut(s) 1381
TfiI GAWTC 6 cut(s) 140, 218, 228, 403, 797, 1022
Tru1I TTAA 4 cut(s) 342, 515, 888, 1307
Tru9I TTAA 4 cut(s) 342, 515, 888, 1307
TscAI CASTG 2 cut(s) 556, 982
TseFI GTSAC 1 cut(s) 1167
TseI GCWGC 4 cut(s) 398, 650, 905, 1192
Tsp45I GTSAC 1 cut(s) 1167
TspDTI ATGAA 4 cut(s) 384, 694, 817, 867
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 2 cut(s) 556, 982
Van91I CCANNNNNTGG 1 cut(s) 1310
XapI RAATTY 3 cut(s) 671, 824, 854
XbaI TCTAGA 1 cut(s) 1202
XmnI GAANNNNTTC 2 cut(s) 71, 1215
XspI CTAG 3 cut(s) 174, 1149, 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.