Rw5G026720

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013187
Physical Location & Seq
Forward (+)
38798254 .. 38802382
4129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026720.1

Sequence Viewer

Length: 1452 bp
ATGGCGACGAGAGCGGAAACAATCTCAGCGGCTATGCAACTGATCGACCCAAAGAAAGAGAACCTAAAGAAGGCCTTCGACGACCTTCAATCCCACTCCGACGTCCTCTCCTCTTCTCTCTCACCTGCAAAGGACCCGAATTTGTCGACTCAGGTAGCGAAAGAGTCTTCTTCTTCATTGAACCCACCGAGCCGGAACAGTGTTGAGCTCAACAATGGTCATGATGCAGATGCAGTGTCTCCTCGGCCTGAGTTGGTGGCGTTTTGCGAGAAAATGGATTCAATTGGGCTGAGGAAGTATATGAATGACACTAAAAATAACCGGAACACGATCCGGTCCGAGCTCCATGGGGCTTTACGGCACGCTCAGGACCCGGCGTTGATGGTTCTCAATGCAATGGAGGGGTTTTACGTGGAGAATAAGGGTGATAAGGACCTGGAAGTGAGTAGTGTGAGGAGAACTGGTGTGCTGTTGTTGGAAGTGTTGTGTGGAGTTTCGCCAAATGTAGGAGTGGAGGTGAGGGAGAGAGCCAAGAAGTTGGCATTGGAGTGGAAAGGGAAGGTTAGTATGGAGGGAGAGAACCAGTTTGAGGCGTTGGGATTCTTGCATTTGGTGGCAGCTTATGGACTGGTGTCTGAATTCAACATGGATGAGCTTGTGGATCATTTGGTTGTTGTTGCAAGGTATAGGCAAGCTACTGATTTGTGCCGCAGACTTGGCTTGGGAGATAAGCTTGCAGATCTGATTCAGAAACTCATGAGCAATGGCAAACATCTTTTGGCTATCAAGTTCATTTCTGAATTTGATATGACTGATAAGTTTCCACCTGTTCCCATCTTAAAAGCGTATGTGAAGGAGTCTAAAAGGTTGGCCAAGAAAGTTTGTGAGGAAGGAAATAACTCTAAGAAGGCGGCGAATGAAGCCATAGCCAAAGAAACCGGTGCTCTGAAATCAGTAATTAAAATTATTGAGGACCTCAAGCTCGAATCTGAATATCCACCGTCTTCTCTTGAAAAGCGAATTGACCAGTTGGCGAAGGAGAAGGCAAACAGAAAACGGCAAGCAGGAGCTCCTGCTGGGAAGCCTTTTCAACTGCAGCAGCAGTATGGTAACAAGCGTCCTCGAATGACTGCTCCTGTTGGCTCCACAGCAGTCTCAAATGGTGCAGCAGGTTTGTTGCTAGAGTCTCTTGCTCCATATGTGAGCTCTGCAGCTGCACCCTATGTCGTGTCGGGCACTGCTCCTCCAGTTGCCCCTTATGTGGGTTCATCAACTGGGCAGTATGGTTTTGCAGGAGGCCAGATGGGTTTTCCTGGGAACCCAGGCCCCTCTAGTACCCATTTATACTCATCCGAACAGTATGTGCCCTCGGGCTATTACAACAGGCTAACTGCCTATGGTGGATATGGTGTACCCCCTCAATACCATCCATCTTACTATTCTCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0010228 GO:0022414 GO:0032501 GO:0032502 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

483

Amino Acids

52.58

Weight (kDa)

8.81

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 79 - 355 2.2e-100 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31814 AT5G16320
fragaria_vesca FvH4_3g24000
malus_domestica MD11G1205100.v1.1
prunus_persica Prupe.4G232500_v2.0.a1
pyrus_communis pycom11g17780
rosa_chinensis RchiOBHm_Chr5g0042571
rosa_laevigata RLG00000034141
rosa_multiflora Rmu_sc0004704.1_g000003
rosa_roxburghii Rroxscaffold_1G00038070
rosa_rugosa Rorug05G0202600 Rorug05G0202700 Rorug05G0202800
rosa_samantha Rh5AG287800 Rh5BG292200 Rh5CG324600 Rh5DG302000
rosa_wichuraiana Rw5G026720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
AatII GACGTC 1 cut(s) 105
Acc36I ACCTGC 2 cut(s) 133, 1160
AccBSI CCGCTC 1 cut(s) 14
AccI GTMKAC 1 cut(s) 146
AciI CCGC 4 cut(s) 14, 29, 709, 911
AclWI GGATC 2 cut(s) 325, 669
AcoI YGGCCR 1 cut(s) 870
AcsI RAATTY 3 cut(s) 139, 638, 800
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 2 cut(s) 1336, 1413
AfiI CCNNNNNNNGG 5 cut(s) 70, 506, 589, 1261, 1262
AgeI ACCGGT 1 cut(s) 938
AgsI TTSAA 6 cut(s) 89, 181, 282, 643, 1013, 1091
AjnI CCWGG 3 cut(s) 435, 1312, 1321
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
Alw21I GWGCWC 5 cut(s) 210, 345, 946, 1072, 1208
Alw26I GTCTC 3 cut(s) 243, 1159, 1191
AlwI GGATC 2 cut(s) 325, 669
Ama87I CYCGRG 1 cut(s) 1369
AoxI GGCC 5 cut(s) 72, 245, 870, 1297, 1324
ApeKI GCWGC 6 cut(s) 617, 1096, 1099, 1166, 1211, 1214
ApoI RAATTY 3 cut(s) 139, 638, 800
AsiGI ACCGGT 1 cut(s) 938
Asp700I GAANNNNTTC 1 cut(s) 74
AspS9I GGNCC 6 cut(s) 133, 336, 370, 433, 973, 1325
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 3 cut(s) 114, 437, 529
AvaI CYCGRG 1 cut(s) 1369
AvaII GGWCC 5 cut(s) 133, 336, 370, 433, 973
BaeGI GKGCMC 2 cut(s) 1238, 1368
BalI TGGCCA 1 cut(s) 872
BanII GRGCYC 4 cut(s) 210, 345, 1072, 1208
BbsI GAAGAC 2 cut(s) 159, 996
Bbv12I GWGCWC 5 cut(s) 210, 345, 946, 1072, 1208
BbvCI CCTCAGC 1 cut(s) 290
BbvI GCAGC 6 cut(s) 629, 1108, 1111, 1178, 1201, 1223
BccI CCATC 5 cut(s) 376, 842, 1297, 1434, 1438
BceAI ACGGC 2 cut(s) 374, 1073
BciT130I CCWGG 3 cut(s) 437, 1314, 1323
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 3 cut(s) 243, 1159, 1191
BfaI CTAG 2 cut(s) 1181, 1332
BfmI CTRYAG 2 cut(s) 1094, 1209
BfuAI ACCTGC 2 cut(s) 133, 1160
BglII AGATCT 1 cut(s) 739
BisI GCNGC 9 cut(s) 30, 618, 709, 912, 1097, 1100, 1167, 1212, 1215
BlsI GCNGC 9 cut(s) 31, 619, 710, 913, 1098, 1101, 1168, 1213, 1216
Bme1390I CCNGG 4 cut(s) 374, 437, 1314, 1323
Bme18I GGWCC 5 cut(s) 133, 336, 370, 433, 973
BmeT110I CYCGRG 1 cut(s) 1369
BmgT120I GGNCC 6 cut(s) 133, 336, 370, 433, 973, 1325
BmiI GGNNCC 5 cut(s) 135, 372, 1144, 1319, 1327
BmrFI CCNGG 4 cut(s) 374, 437, 1314, 1323
BmrI ACTGGG 1 cut(s) 1284
BmsI GCATC 2 cut(s) 214, 220
BmuI ACTGGG 1 cut(s) 1284
BoxI GACNNNNGTC 1 cut(s) 631
BpiI GAAGAC 2 cut(s) 159, 996
BpmI CTGGAG 1 cut(s) 1230
Bpu10I CCTNAGC 2 cut(s) 290, 366
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 412
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 5 cut(s) 242, 346, 1313, 1321, 1368
BsaWI WCCGGW 3 cut(s) 321, 333, 938
BsaXI ACNNNNNCTCC 8 cut(s) 92, 122, 448, 478, 501, 531, 1228, 1258
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 506, 589, 1261, 1262
Bse118I RCCGGY 1 cut(s) 938
Bse1I ACTGG 6 cut(s) 466, 583, 633, 1027, 1247, 1279
Bse3DI GCAATG 2 cut(s) 402, 769
BseBI CCWGG 3 cut(s) 437, 1314, 1323
BseDI CCNNGG 5 cut(s) 242, 346, 1313, 1321, 1368
BseGI GGATG 3 cut(s) 655, 1349, 1426
BseLI CCNNNNNNNGG 5 cut(s) 70, 506, 589, 1261, 1262
BseMI GCAATG 2 cut(s) 402, 769
BseMII CTCAG 5 cut(s) 39, 164, 240, 281, 380
BseNI ACTGG 6 cut(s) 466, 583, 633, 1027, 1247, 1279
BseRI GAGGAG 4 cut(s) 100, 231, 469, 1233
BseSI GKGCMC 2 cut(s) 1238, 1368
BseXI GCAGC 6 cut(s) 629, 1108, 1111, 1178, 1201, 1223
BseYI CCCAGC 1 cut(s) 1076
BsgI GTGCAG 2 cut(s) 1185, 1200
BshFI GGCC 5 cut(s) 74, 247, 872, 1299, 1326
BshTI ACCGGT 1 cut(s) 938
BsiHKAI GWGCWC 5 cut(s) 210, 345, 946, 1072, 1208
BsiHKCI CYCGRG 1 cut(s) 1369
BsiSI CCGG 5 cut(s) 193, 322, 334, 374, 939
BslI CCNNNNNNNGG 5 cut(s) 70, 506, 589, 1261, 1262
BsmAI GTCTC 3 cut(s) 243, 1159, 1191
BsnI GGCC 5 cut(s) 74, 247, 872, 1299, 1326
BsoBI CYCGRG 1 cut(s) 1369
Bsp1286I GDGCHC 7 cut(s) 210, 345, 946, 1072, 1208, 1238, 1368
Bsp143I GATC 4 cut(s) 42, 330, 661, 739
Bsp19I CCATGG 1 cut(s) 346
BspACI CCGC 4 cut(s) 14, 29, 709, 911
BspANI GGCC 5 cut(s) 74, 247, 872, 1299, 1326
BspCNI CTCAG 5 cut(s) 38, 163, 241, 282, 379
BspHI TCATGA 2 cut(s) 220, 756
BspLI GGNNCC 5 cut(s) 135, 372, 1144, 1319, 1327
BspMAI CTGCAG 2 cut(s) 1098, 1213
BspMI ACCTGC 2 cut(s) 133, 1160
BspPI GGATC 2 cut(s) 325, 669
BsrBI CCGCTC 1 cut(s) 14
BsrDI GCAATG 2 cut(s) 402, 769
BsrFI RCCGGY 1 cut(s) 938
BsrI ACTGG 6 cut(s) 466, 583, 633, 1027, 1247, 1279
BssAI RCCGGY 1 cut(s) 938
BssECI CCNNGG 5 cut(s) 242, 346, 1313, 1321, 1368
BssMI GATC 4 cut(s) 42, 330, 661, 739
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 3 cut(s) 437, 1314, 1323
Bst4CI ACNGT 3 cut(s) 200, 1002, 1359
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 412
BstC8I GCNNGC 4 cut(s) 363, 693, 735, 1062
BstDEI CTNAG 7 cut(s) 25, 150, 249, 290, 366, 903, 1443
BstDSI CCRYGG 1 cut(s) 346
BstENI CCTNNNNNAGG 1 cut(s) 68
BstF5I GGATG 3 cut(s) 655, 1349, 1426
BstKTI GATC 4 cut(s) 45, 333, 664, 742
BstMAI GTCTC 3 cut(s) 243, 1159, 1191
BstMBI GATC 4 cut(s) 42, 330, 661, 739
BstMWI GCNNNNNNNGC 3 cut(s) 11, 717, 920
BstNI CCWGG 3 cut(s) 437, 1314, 1323
BstPAI GACNNNNGTC 1 cut(s) 631
BstSCI CCNGG 4 cut(s) 372, 435, 1312, 1321
BstSFI CTRYAG 2 cut(s) 1094, 1209
BstSLI GKGCMC 2 cut(s) 1238, 1368
BstV1I GCAGC 6 cut(s) 629, 1108, 1111, 1178, 1201, 1223
BstV2I GAAGAC 2 cut(s) 159, 996
BstX2I RGATCY 1 cut(s) 739
BstXI CCANNNNNNTGG 1 cut(s) 538
BstYI RGATCY 1 cut(s) 739
BsuRI GGCC 5 cut(s) 74, 247, 872, 1299, 1326
BtgI CCRYGG 1 cut(s) 346
BtsCI GGATG 3 cut(s) 655, 1349, 1426
BtsI GCAGTG 2 cut(s) 240, 1236
BtsIMutI CAGTG 3 cut(s) 205, 240, 1236
BveI ACCTGC 2 cut(s) 133, 1160
Cac8I GCNNGC 4 cut(s) 363, 693, 735, 1062
CciI TCATGA 2 cut(s) 220, 756
Cfr10I RCCGGY 1 cut(s) 938
Cfr13I GGNCC 6 cut(s) 133, 336, 370, 433, 973, 1325
CpoI CGGWCCG 1 cut(s) 336
CseI GACGC 1 cut(s) 1106
Csp6I GTAC 2 cut(s) 1335, 1412
CspAI ACCGGT 1 cut(s) 938
CspI CGGWCCG 1 cut(s) 336
CviAII CATG 4 cut(s) 221, 347, 646, 757
CviQI GTAC 2 cut(s) 1335, 1412
DdeI CTNAG 7 cut(s) 25, 150, 249, 290, 366, 903, 1443
DpnI GATC 4 cut(s) 44, 332, 663, 741
DpnII GATC 4 cut(s) 42, 330, 661, 739
EaeI YGGCCR 1 cut(s) 870
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Ecl136II GAGCTC 4 cut(s) 208, 343, 1070, 1206
Eco130I CCWWGG 1 cut(s) 346
Eco147I AGGCCT 1 cut(s) 74
Eco24I GRGCYC 4 cut(s) 210, 345, 1072, 1208
Eco47I GGWCC 5 cut(s) 133, 336, 370, 433, 973
Eco53kI GAGCTC 4 cut(s) 208, 343, 1070, 1206
Eco88I CYCGRG 1 cut(s) 1369
EcoICRI GAGCTC 4 cut(s) 208, 343, 1070, 1206
EcoNI CCTNNNNNAGG 1 cut(s) 68
EcoO109I RGGNCCY 5 cut(s) 133, 370, 433, 973, 1325
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 3 cut(s) 435, 1312, 1321
EcoT14I CCWWGG 1 cut(s) 346
EcoT38I GRGCYC 4 cut(s) 210, 345, 1072, 1208
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 4 cut(s) 224, 350, 649, 760
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FatI CATG 4 cut(s) 220, 346, 645, 756
FauNDI CATATG 1 cut(s) 1198
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 9 cut(s) 30, 618, 709, 912, 1097, 1100, 1167, 1212, 1215
FokI GGATG 3 cut(s) 662, 1336, 1413
FriOI GRGCYC 4 cut(s) 210, 345, 1072, 1208
Fsp4HI GCNGC 9 cut(s) 30, 618, 709, 912, 1097, 1100, 1167, 1212, 1215
FspBI CTAG 2 cut(s) 1181, 1332
GluI GCNGC 9 cut(s) 30, 618, 709, 912, 1097, 1100, 1167, 1212, 1215
GsaI CCCAGC 1 cut(s) 1080
GsuI CTGGAG 1 cut(s) 1230
HaeIII GGCC 5 cut(s) 74, 247, 872, 1299, 1326
HapII CCGG 5 cut(s) 193, 322, 334, 374, 939
HgaI GACGC 1 cut(s) 1106
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 4 cut(s) 224, 350, 649, 760
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 8 cut(s) 148, 164, 278, 600, 745, 857, 986, 1184
HpaII CCGG 5 cut(s) 193, 322, 334, 374, 939
HphI GGTGA 3 cut(s) 114, 437, 529
Hpy166II GTNNAC 2 cut(s) 147, 1412
Hpy188I TCNGA 9 cut(s) 100, 340, 637, 744, 750, 799, 948, 991, 1354
Hpy188III TCNNGA 4 cut(s) 221, 368, 757, 1010
Hpy8I GTNNAC 2 cut(s) 147, 1412
Hpy99I CGWCG 3 cut(s) 10, 83, 104
HpyAV CCTTC 9 cut(s) 64, 85, 95, 553, 847, 884, 901, 1030, 1036
HpyCH4III ACNGT 3 cut(s) 200, 1002, 1359
HpyCH4IV ACGT 2 cut(s) 102, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 717, 920
HpyF3I CTNAG 7 cut(s) 25, 150, 249, 290, 366, 903, 1443
HpySE526I ACGT 2 cut(s) 102, 411
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 4 cut(s) 224, 350, 649, 760
Kzo9I GATC 4 cut(s) 42, 330, 661, 739
LmnI GCTCC 7 cut(s) 348, 1067, 1075, 1138, 1148, 1198, 1246
Lsp1109I GCAGC 6 cut(s) 629, 1108, 1111, 1178, 1201, 1223
LweI GCATC 2 cut(s) 214, 220
MaeI CTAG 2 cut(s) 1181, 1332
MaeII ACGT 2 cut(s) 102, 411
MaeIII GTNAC 1 cut(s) 1109
MalI GATC 4 cut(s) 44, 332, 663, 741
MbiI CCGCTC 1 cut(s) 14
MboI GATC 4 cut(s) 42, 330, 661, 739
MboII GAAGA 5 cut(s) 105, 159, 162, 165, 996
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 7 cut(s) 210, 345, 946, 1072, 1208, 1238, 1368
MlsI TGGCCA 1 cut(s) 872
MluCI AATT 7 cut(s) 139, 282, 638, 800, 957, 963, 1020
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 4 cut(s) 142, 173, 866, 1193
MmeI TCCRAC 2 cut(s) 123, 456
Mox20I TGGCCA 1 cut(s) 872
MroXI GAANNNNTTC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 872
MseI TTAA 2 cut(s) 839, 960
MslI CAYNNNNRTG 1 cut(s) 547
Msp20I TGGCCA 1 cut(s) 872
MspA1I CMGCKG 2 cut(s) 29, 1214
MspI CCGG 5 cut(s) 193, 322, 334, 374, 939
MspR9I CCNGG 4 cut(s) 374, 437, 1314, 1323
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 3 cut(s) 437, 1314, 1323
MwoI GCNNNNNNNGC 3 cut(s) 11, 717, 920
NciI CCSGG 1 cut(s) 374
NcoI CCATGG 1 cut(s) 346
NdeI CATATG 1 cut(s) 1198
NdeII GATC 4 cut(s) 42, 330, 661, 739
NlaIII CATG 4 cut(s) 224, 350, 649, 760
NlaIV GGNNCC 5 cut(s) 135, 372, 1144, 1319, 1327
NmeAIII GCCGAG 1 cut(s) 223
PagI TCATGA 2 cut(s) 220, 756
PaqCI CACCTGC 1 cut(s) 133
PceI AGGCCT 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 4 cut(s) 278, 600, 745, 986
PinAI ACCGGT 1 cut(s) 938
PkrI GCNGC 9 cut(s) 31, 619, 710, 913, 1098, 1101, 1168, 1213, 1216
PleI GAGTC 4 cut(s) 142, 172, 865, 1192
PpsI GAGTC 4 cut(s) 142, 172, 865, 1192
Ppu21I YACGTR 1 cut(s) 412
PpuMI RGGWCCY 4 cut(s) 133, 370, 433, 973
PshAI GACNNNNGTC 1 cut(s) 631
Psp124BI GAGCTC 4 cut(s) 210, 345, 1072, 1208
Psp5II RGGWCCY 4 cut(s) 133, 370, 433, 973
Psp6I CCWGG 3 cut(s) 435, 1312, 1321
PspFI CCCAGC 1 cut(s) 1076
PspGI CCWGG 3 cut(s) 435, 1312, 1321
PspN4I GGNNCC 5 cut(s) 135, 372, 1144, 1319, 1327
PspPI GGNCC 6 cut(s) 133, 336, 370, 433, 973, 1325
PspPPI RGGWCCY 4 cut(s) 133, 370, 433, 973
PstI CTGCAG 2 cut(s) 1098, 1213
PsuI RGATCY 1 cut(s) 739
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 2 cut(s) 1336, 1413
RsaNI GTAC 2 cut(s) 1335, 1412
RseI CAYNNNNRTG 1 cut(s) 547
Rsr2I CGGWCCG 1 cut(s) 336
RsrII CGGWCCG 1 cut(s) 336
SacI GAGCTC 4 cut(s) 210, 345, 1072, 1208
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 839, 960
SatI GCNGC 9 cut(s) 30, 618, 709, 912, 1097, 1100, 1167, 1212, 1215
Sau3AI GATC 4 cut(s) 42, 330, 661, 739
Sau96I GGNCC 6 cut(s) 133, 336, 370, 433, 973, 1325
SchI GAGTC 4 cut(s) 142, 173, 866, 1193
ScrFI CCNGG 4 cut(s) 374, 437, 1314, 1323
SduI GDGCHC 7 cut(s) 210, 345, 946, 1072, 1208, 1238, 1368
SfaNI GCATC 2 cut(s) 214, 220
SfcI CTRYAG 2 cut(s) 1094, 1209
SinI GGWCC 5 cut(s) 133, 336, 370, 433, 973
SmiMI CAYNNNNRTG 1 cut(s) 547
SmlI CTYRAG 1 cut(s) 977
SmoI CTYRAG 1 cut(s) 977
Sse9I AATT 7 cut(s) 139, 282, 638, 800, 957, 963, 1020
SseBI AGGCCT 1 cut(s) 74
SsiI CCGC 4 cut(s) 14, 29, 709, 911
SspMI CTAG 2 cut(s) 1181, 1332
SstI GAGCTC 4 cut(s) 210, 345, 1072, 1208
StuI AGGCCT 1 cut(s) 74
StyD4I CCNGG 4 cut(s) 372, 435, 1312, 1321
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 3 cut(s) 200, 1002, 1359
TaiI ACGT 2 cut(s) 105, 414
TaqI TCGA 5 cut(s) 45, 78, 146, 984, 1123
TasI AATT 7 cut(s) 139, 282, 638, 800, 957, 963, 1020
TauI GCSGC 3 cut(s) 32, 711, 914
TfiI GAWTC 4 cut(s) 278, 600, 745, 986
Tru1I TTAA 2 cut(s) 839, 960
Tru9I TTAA 2 cut(s) 839, 960
TscAI CASTG 3 cut(s) 205, 240, 1243
TseI GCWGC 6 cut(s) 617, 1096, 1099, 1166, 1211, 1214
TspDTI ATGAA 5 cut(s) 165, 317, 781, 933, 1257
TspRI CASTG 3 cut(s) 205, 240, 1243
VpaK11BI GGWCC 5 cut(s) 133, 336, 370, 433, 973
XagI CCTNNNNNAGG 1 cut(s) 68
XapI RAATTY 3 cut(s) 139, 638, 800
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 2 cut(s) 1181, 1332
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.