AT5G19473

Cleavage site for pathogenic type III effector avirulence factor Avr

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012034
Physical Location & Seq
Forward (+)
6569162 .. 6570956
1795 bp
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UTR
Exon/CDS
Intron
AT5G19473.2

Sequence Viewer

Length: 300 bp
ATGGATAAAAGAAGGGAGAATGCTGCATGGACTCCAGTTCCACAGTTCGGAGGGTGGGACCAAAAAGGACCAAATGATGCTACAAACTACTCGGTTGTCTTTTCTAAAGCTCGCGCAAACAGGAAGCAGAACAAAGCTGGTGTAAGACATTCTAGTTTGGGAAGCGAACAAGAACTCATGGTAAGTGTTCGTCGCAACCACCAACAACTTCACCATCGCCATGAAACTCAAGACGATGACCCTGTCATGAAGAAGAAGAGGATCTTGACCTACATTAATTGTTGTATAAGGCCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

99

Amino Acids

11.51

Weight (kDa)

10.01

Isoelectric Point (pI)

57.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 44 - 77 8.5e-12 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19473 AT5G19473
fragaria_vesca FvH4_3g27760 FvH4_3g27760
malus_domestica MD03G1164700.v1.1
prunus_persica Prupe.4G264200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0050361
rosa_laevigata RLG00000034699
rosa_multiflora Rmu_sc0005060.1_g000015
rosa_roxburghii Rroxscaffold_1G00030580
rosa_rugosa Rorug05G0260100 Rorug05G0260200 Rorug05G0260300 Rorug05G0260400
rosa_samantha Rh5AG332100 Rh5BG342400 Rh5CG369600 Rh5DG355200
rosa_wichuraiana Rw5G031390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 114
AclWI GGATC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 47
AluBI AGCT 2 cut(s) 110, 137
AluI AGCT 2 cut(s) 110, 137
AlwI GGATC 1 cut(s) 269
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 23
AseI ATTAAT 1 cut(s) 276
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 2 cut(s) 58, 68
AsuHPI GGTGA 1 cut(s) 203
AvaII GGWCC 2 cut(s) 58, 68
BbvI GCAGC 1 cut(s) 10
BccI CCATC 1 cut(s) 222
BfaI CTAG 2 cut(s) 153, 298
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
Bme18I GGWCC 2 cut(s) 58, 68
BmgT120I GGNCC 2 cut(s) 58, 68
BmiI GGNNCC 1 cut(s) 59
BmsI GCATC 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 213
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 292
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 1 cut(s) 292
Bsp143I GATC 1 cut(s) 261
BspANI GGCC 1 cut(s) 292
BspFNI CGCG 1 cut(s) 114
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 59
BspPI GGATC 1 cut(s) 269
BsrI ACTGG 1 cut(s) 35
BssMI GATC 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 112
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 1 cut(s) 264
BstMBI GATC 1 cut(s) 261
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 1 cut(s) 292
BtgZI GCGATG 1 cut(s) 200
Cac8I GCNNGC 1 cut(s) 112
CciI TCATGA 1 cut(s) 246
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 58, 68
CviAII CATG 4 cut(s) 27, 178, 221, 247
CviJI RGCY 3 cut(s) 110, 137, 292
CviKI_1 RGCY 3 cut(s) 110, 137, 292
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco47I GGWCC 2 cut(s) 58, 68
FaeI CATG 4 cut(s) 30, 181, 224, 250
FaiI YATR 5 cut(s) 28, 179, 222, 248, 287
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FaqI GGGAC 1 cut(s) 71
FatI CATG 4 cut(s) 26, 177, 220, 246
Fnu4HI GCNGC 1 cut(s) 24
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 2 cut(s) 153, 298
GlaI GCGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 18
HaeIII GGCC 1 cut(s) 292
HhaI GCGC 1 cut(s) 116
Hin1II CATG 4 cut(s) 30, 181, 224, 250
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HinfI GANTC 1 cut(s) 31
HphI GGTGA 1 cut(s) 203
Hpy188I TCNGA 1 cut(s) 50
Hpy188III TCNNGA 3 cut(s) 230, 247, 265
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 1 cut(s) 6
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4V TGCA 1 cut(s) 26
Hsp92II CATG 4 cut(s) 30, 181, 224, 250
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 1 cut(s) 261
LpnPI CCDG 4 cut(s) 48, 106, 123, 255
Lsp1109I GCAGC 1 cut(s) 10
LweI GCATC 1 cut(s) 67
MaeI CTAG 2 cut(s) 153, 298
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 3 cut(s) 262, 265, 268
MflI RGATCY 1 cut(s) 261
MluCI AATT 1 cut(s) 277
MlyI GAGTC 1 cut(s) 25
MnlI CCTC 2 cut(s) 44, 252
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 25
MvnI CGCG 1 cut(s) 114
NdeII GATC 1 cut(s) 261
NlaIII CATG 4 cut(s) 30, 181, 224, 250
NlaIV GGNNCC 1 cut(s) 59
PagI TCATGA 1 cut(s) 246
PctI GAATGC 1 cut(s) 25
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 1 cut(s) 25
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PshBI ATTAAT 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 59
PspPI GGNCC 2 cut(s) 58, 68
PsuI RGATCY 1 cut(s) 261
PsyI GACNNNGTC 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 2 cut(s) 58, 68
SchI GAGTC 1 cut(s) 25
SetI ASST 3 cut(s) 112, 139, 272
SfaNI GCATC 1 cut(s) 67
SinI GGWCC 2 cut(s) 58, 68
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
Sse9I AATT 1 cut(s) 277
SspMI CTAG 2 cut(s) 153, 298
TaaI ACNGT 1 cut(s) 45
TasI AATT 1 cut(s) 277
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TseI GCWGC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 237, 263
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 58, 68
VspI ATTAAT 1 cut(s) 276
XspI CTAG 2 cut(s) 153, 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.