MD03G1164700.v1.1

RPM1-interacting protein 4 (RIN4) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012034
Physical Location & Seq
Forward (+)
21707970 .. 21709609
1640 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1164700.v1.1.491

Sequence Viewer

Length: 276 bp
ATGCATTTGCAGAAAAATGGTCGGAAGTCTGTACCTCAATTCGGAGGATGGGATCAGAACAGCCCAGATACAAACTACACCGTTGTGTTCTCTCAGGCTCGTGCTAACCGGAAGCAAAACAAGACCAACTTGACCGAGTTCAAGCGTAACAGCCTTGGGAATGAACAGGAATTCATGGCTGCTCACCCTCATCATCCTCATCATCATCACCAAGATGACTCTGTTACGAGGAAAAAGACGATTCTGACTTACATAAATTGCTGTATTAGGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

92

Amino Acids

10.62

Weight (kDa)

9.76

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 7 - 38 1.7e-12 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19473 AT5G19473
fragaria_vesca FvH4_3g27760 FvH4_3g27760
malus_domestica MD03G1164700.v1.1
prunus_persica Prupe.4G264200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0050361
rosa_laevigata RLG00000034699
rosa_multiflora Rmu_sc0005060.1_g000015
rosa_roxburghii Rroxscaffold_1G00030580
rosa_rugosa Rorug05G0260100 Rorug05G0260200 Rorug05G0260300 Rorug05G0260400
rosa_samantha Rh5AG332100 Rh5BG342400 Rh5CG369600 Rh5DG355200
rosa_wichuraiana Rw5G031390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 170
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 41
AgsI TTSAA 1 cut(s) 142
AleI CACNNNNGTG 1 cut(s) 83
AlwI GGATC 1 cut(s) 60
AoxI GGCC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 1 cut(s) 170
AsuHPI GGTGA 2 cut(s) 176, 200
BauI CACGAG 1 cut(s) 99
BbvI GCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 42
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 154
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 2 cut(s) 53, 193
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 166
BshFI GGCC 1 cut(s) 271
BsiSI CCGG 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 41
BsnI GGCC 1 cut(s) 271
Bsp143I GATC 1 cut(s) 52
BspANI GGCC 1 cut(s) 271
BspCNI CTCAG 1 cut(s) 106
BspPI GGATC 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 1 cut(s) 52
BssSI CACGAG 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 154
Bst2BI CACGAG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 82
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 2 cut(s) 53, 193
BstKTI GATC 1 cut(s) 55
BstMBI GATC 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 166
BsuRI GGCC 1 cut(s) 271
BtsCI GGATG 2 cut(s) 53, 193
Csp6I GTAC 1 cut(s) 32
CviAII CATG 1 cut(s) 175
CviJI RGCY 5 cut(s) 63, 98, 153, 179, 271
CviKI_1 RGCY 5 cut(s) 63, 98, 153, 179, 271
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 1 cut(s) 93
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 154
Eco147I AGGCCT 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 1 cut(s) 178
FaiI YATR 2 cut(s) 176, 254
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 2 cut(s) 60, 180
Fsp4HI GCNGC 1 cut(s) 180
GluI GCNGC 1 cut(s) 180
HaeIII GGCC 1 cut(s) 271
HapII CCGG 1 cut(s) 109
Hin1II CATG 1 cut(s) 178
HinfI GANTC 2 cut(s) 218, 241
HpaII CCGG 1 cut(s) 109
HphI GGTGA 2 cut(s) 176, 200
Hpy188I TCNGA 4 cut(s) 24, 44, 57, 246
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4V TGCA 2 cut(s) 4, 10
HpyF3I CTNAG 1 cut(s) 93
Hsp92II CATG 1 cut(s) 178
Kzo9I GATC 1 cut(s) 52
LpnPI CCDG 4 cut(s) 78, 80, 122, 152
Lsp1109I GCAGC 1 cut(s) 166
MaeIII GTNAC 2 cut(s) 146, 223
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MluCI AATT 3 cut(s) 38, 170, 256
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 5 cut(s) 38, 45, 198, 207, 222
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 2 cut(s) 83, 213
MspI CCGG 1 cut(s) 109
NdeII GATC 1 cut(s) 52
NlaIII CATG 1 cut(s) 178
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 83
PceI AGGCCT 1 cut(s) 271
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 1 cut(s) 181
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
RseI CAYNNNNRTG 2 cut(s) 83, 213
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 1 cut(s) 52
SchI GAGTC 1 cut(s) 212
SetI ASST 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 83, 213
Sse9I AATT 3 cut(s) 38, 170, 256
SseBI AGGCCT 1 cut(s) 271
StuI AGGCCT 1 cut(s) 271
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 1 cut(s) 82
TaqII GACCGA 1 cut(s) 149
TasI AATT 3 cut(s) 38, 170, 256
TfiI GAWTC 1 cut(s) 241
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 2 cut(s) 163, 177
XapI RAATTY 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.