Rorug05G0260300

Cleavage site for pathogenic type III effector avirulence factor Avr

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012034
Physical Location & Seq
Reverse (-)
29375120 .. 29378024
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0260300.1

Sequence Viewer

Length: 1032 bp
ATGTGGCTAGCGGACGTAGCTATGCATGCCAAGGTGAGCTGCATGCTTGCAGGGGTGGGCACGTGTGTTAGGCTGCCTACGTACCCCGGGAGGGATCAAGCCACTCGTAGTTCAATTTTTGTGGCTTTAGGGTTTCAACAGCTCGGGTATTTGGAAATGGAAAACCATAGCGTCATGCTCTGCCGAATTCTTCAAATGGGCCTAAATTTAGACATGGGCCTCCTTCGTATCCTTGCGAATCATGTTCCTCGATCCAATTCGGAGACATGCATCAAACTTCTACTTTGGGTATTAGAAAGACTTTTTGAAGGTCCAGACACCCATCAAACTGTTCAAAGGCCCAATCTTCCCAGAACATATCGCCCCCGTCTTAACCCAAACCAAGTAGAACACGAGCTCAATACTGATCTTCTGCGAAGTCGAATTAGAGATGGATTTGAAGACAGAGGCCAAATCGGAGATGGAACAATACATGGAAGGCCAGATACTAACGCCCATTCTTCTTCCACAACATTTCACAGTGATTTTGAAAAAGAGGAGGTTTGTGCATCGGGGAGCCATCTTCAAAGGTCGGCATATTGTGGGTACAAATCAAGTACAGGAGCTGTGGTGTCACAAATTCCGGAGTTCCAGAATTTGATTCAATTGGTGCGTCTTCGATCGTTTTCTCTTTGTTTCTTTGCACCGAAAACATTTATCTTTGTGCTTTGCCAACTCCTAAAGCTGCAATTTGAAGGATTTGAATTTGATAACCCTTTGTGCAGGTATAAGGTGAGGACAGCAACTGCAGTCAAGACTCCAGAGTTTCAATTTGAGGACCATCGGAGACGCTGCTCCGGTATATTCATCGGGACGCTGCCCAGCATGATTCATCGGGACGCTGCCCGGCATGTTCATCGGAGACGCTGCTCCGGCATGTTCATCAGAGACGCTGCTCTGACATGTTTTCTTTTATGCAGCATTGTTGAGCAACCTGTGAACACAAAGAAAGTGGAGATCATGGTAGGGAGGGAGTCTCAACCTTTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

343

Amino Acids

39.1

Weight (kDa)

8.91

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19473 AT5G19473
fragaria_vesca FvH4_3g27760 FvH4_3g27760
malus_domestica MD03G1164700.v1.1
prunus_persica Prupe.4G264200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0050361
rosa_laevigata RLG00000034699
rosa_multiflora Rmu_sc0005060.1_g000015
rosa_roxburghii Rroxscaffold_1G00030580
rosa_rugosa Rorug05G0260100 Rorug05G0260200 Rorug05G0260300 Rorug05G0260400
rosa_samantha Rh5AG332100 Rh5BG342400 Rh5CG369600 Rh5DG355200
rosa_wichuraiana Rw5G031390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 753
AccIII TCCGGA 1 cut(s) 622
AciI CCGC 1 cut(s) 11
AclWI GGATC 2 cut(s) 102, 246
AcsI RAATTY 5 cut(s) 186, 205, 618, 634, 743
AcvI CACGTG 1 cut(s) 63
AfaI GTAC 3 cut(s) 83, 587, 598
AfiI CCNNNNNNNGG 1 cut(s) 91
AflIII ACRYGT 2 cut(s) 62, 941
AluBI AGCT 6 cut(s) 20, 39, 142, 397, 605, 724
AluI AGCT 6 cut(s) 20, 39, 142, 397, 605, 724
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 5 cut(s) 257, 820, 895, 921, 1020
AlwI GGATC 2 cut(s) 102, 246
AlwNI CAGNNNCTG 2 cut(s) 605, 785
Ama87I CYCGRG 2 cut(s) 86, 143
Aor13HI TCCGGA 1 cut(s) 622
AoxI GGCC 5 cut(s) 199, 217, 338, 448, 479
ApeKI GCWGC 9 cut(s) 39, 73, 724, 831, 856, 881, 906, 932, 957
ApoI RAATTY 5 cut(s) 186, 205, 618, 634, 743
AspS9I GGNCC 5 cut(s) 199, 217, 311, 339, 817
AsuC2I CCSGG 3 cut(s) 87, 88, 886
AsuHPI GGTGA 2 cut(s) 46, 784
AsuNHI GCTAGC 1 cut(s) 7
AvaI CYCGRG 2 cut(s) 86, 143
AvaII GGWCC 2 cut(s) 311, 817
BaeGI GKGCMC 1 cut(s) 62
BanII GRGCYC 1 cut(s) 399
BarI GAAGNNNNNNTAC 2 cut(s) 469, 501
BauI CACGAG 1 cut(s) 392
BbrPI CACGTG 1 cut(s) 63
BbsI GAAGAC 2 cut(s) 447, 647
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 9 cut(s) 26, 60, 711, 818, 843, 868, 893, 919, 969
BccI CCATC 5 cut(s) 330, 425, 455, 567, 828
BcgI CGANNNNNNTGC 2 cut(s) 878, 912
BciVI GTATCC 1 cut(s) 239
BcnI CCSGG 3 cut(s) 87, 88, 886
BcoDI GTCTC 5 cut(s) 257, 820, 895, 921, 1020
BfaI CTAG 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 786
BfuAI ACCTGC 1 cut(s) 753
BfuI GTATCC 1 cut(s) 239
BisI GCNGC 9 cut(s) 40, 74, 725, 832, 857, 882, 907, 933, 958
BlsI GCNGC 9 cut(s) 41, 75, 726, 833, 858, 883, 908, 934, 959
Bme1390I CCNGG 3 cut(s) 87, 88, 886
Bme18I GGWCC 2 cut(s) 311, 817
BmeT110I CYCGRG 2 cut(s) 86, 143
BmgT120I GGNCC 5 cut(s) 199, 217, 311, 339, 817
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 3 cut(s) 87, 88, 886
BmsI GCATC 2 cut(s) 279, 557
BmtI GCTAGC 1 cut(s) 11
BpiI GAAGAC 2 cut(s) 447, 647
BplI GAGNNNNNCTC 2 cut(s) 1000, 1032
BpmI CTGGAG 1 cut(s) 783
BpuMI CCSGG 3 cut(s) 87, 88, 886
BsaAI YACGTR 2 cut(s) 63, 81
BsaJI CCNNGG 3 cut(s) 30, 85, 86
BsaWI WCCGGW 2 cut(s) 622, 836
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 91
BseAI TCCGGA 1 cut(s) 622
BseDI CCNNGG 3 cut(s) 30, 85, 86
BseLI CCNNNNNNNGG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 551
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 9 cut(s) 26, 60, 711, 818, 843, 868, 893, 919, 969
BseYI CCCAGC 1 cut(s) 860
BsgI GTGCAG 1 cut(s) 781
Bsh1285I CGRYCG 1 cut(s) 662
BshFI GGCC 5 cut(s) 201, 219, 340, 450, 481
BsiEI CGRYCG 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 399
BsiHKCI CYCGRG 2 cut(s) 86, 143
BsiSI CCGG 5 cut(s) 87, 623, 837, 886, 912
BslFI GGGAC 2 cut(s) 865, 890
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 5 cut(s) 257, 820, 895, 921, 1020
BsmBI CGTCTC 3 cut(s) 820, 895, 921
BsmFI GGGAC 2 cut(s) 865, 890
BsnI GGCC 5 cut(s) 201, 219, 340, 450, 481
BsoBI CYCGRG 2 cut(s) 86, 143
Bsp1286I GDGCHC 2 cut(s) 62, 399
Bsp13I TCCGGA 1 cut(s) 622
Bsp143I GATC 5 cut(s) 94, 251, 406, 659, 996
BspACI CCGC 1 cut(s) 11
BspANI GGCC 5 cut(s) 201, 219, 340, 450, 481
BspEI TCCGGA 1 cut(s) 622
BspLI GGNNCC 1 cut(s) 557
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 1 cut(s) 753
BspOI GCTAGC 1 cut(s) 11
BspPI GGATC 2 cut(s) 102, 246
BssECI CCNNGG 3 cut(s) 30, 85, 86
BssMI GATC 5 cut(s) 94, 251, 406, 659, 996
BssSI CACGAG 1 cut(s) 392
BssT1I CCWWGG 1 cut(s) 30
Bst2BI CACGAG 1 cut(s) 392
Bst4CI ACNGT 2 cut(s) 331, 521
BstBAI YACGTR 2 cut(s) 63, 81
BstC8I GCNNGC 4 cut(s) 9, 27, 44, 48
BstKTI GATC 5 cut(s) 97, 254, 409, 662, 999
BstMAI GTCTC 5 cut(s) 257, 820, 895, 921, 1020
BstMBI GATC 5 cut(s) 94, 251, 406, 659, 996
BstMCI CGRYCG 1 cut(s) 662
BstMWI GCNNNNNNNGC 3 cut(s) 17, 26, 912
BstNSI RCATGY 6 cut(s) 29, 46, 270, 893, 919, 945
BstSCI CCNGG 3 cut(s) 85, 86, 884
BstSFI CTRYAG 1 cut(s) 786
BstSLI GKGCMC 1 cut(s) 62
BstSNI TACGTA 1 cut(s) 81
BstV1I GCAGC 9 cut(s) 26, 60, 711, 818, 843, 868, 893, 919, 969
BstV2I GAAGAC 2 cut(s) 447, 647
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 5 cut(s) 201, 219, 340, 450, 481
BtsIMutI CAGTG 1 cut(s) 526
BveI ACCTGC 1 cut(s) 753
Cac8I GCNNGC 4 cut(s) 9, 27, 44, 48
CaiI CAGNNNCTG 2 cut(s) 605, 785
Cfr13I GGNCC 5 cut(s) 199, 217, 311, 339, 817
Cfr9I CCCGGG 1 cut(s) 86
CseI GACGC 7 cut(s) 160, 641, 837, 862, 887, 912, 938
Csp6I GTAC 3 cut(s) 82, 586, 597
CspCI CAANNNNNGTGG 5 cut(s) 102, 137, 972, 1007, 1007
CviQI GTAC 3 cut(s) 82, 586, 597
DpnI GATC 5 cut(s) 96, 253, 408, 661, 998
DpnII GATC 5 cut(s) 94, 251, 406, 659, 996
Ecl136II GAGCTC 1 cut(s) 397
Eco105I TACGTA 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 2 cut(s) 311, 817
Eco53kI GAGCTC 1 cut(s) 397
Eco72I CACGTG 1 cut(s) 63
Eco88I CYCGRG 2 cut(s) 86, 143
EcoICRI GAGCTC 1 cut(s) 397
EcoRI GAATTC 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 2 cut(s) 27, 272
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 30
Esp3I CGTCTC 3 cut(s) 820, 895, 921
FaqI GGGAC 2 cut(s) 865, 890
Fnu4HI GCNGC 9 cut(s) 40, 74, 725, 832, 857, 882, 907, 933, 958
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 9 cut(s) 40, 74, 725, 832, 857, 882, 907, 933, 958
FspBI CTAG 1 cut(s) 8
GluI GCNGC 9 cut(s) 40, 74, 725, 832, 857, 882, 907, 933, 958
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 5 cut(s) 201, 219, 340, 450, 481
HapII CCGG 5 cut(s) 87, 623, 837, 886, 912
HgaI GACGC 7 cut(s) 160, 641, 837, 862, 887, 912, 938
HinfI GANTC 5 cut(s) 238, 640, 796, 868, 1013
HpaII CCGG 5 cut(s) 87, 623, 837, 886, 912
HphI GGTGA 2 cut(s) 46, 784
Hpy166II GTNNAC 1 cut(s) 979
Hpy188I TCNGA 6 cut(s) 262, 458, 825, 900, 926, 939
Hpy188III TCNNGA 7 cut(s) 314, 623, 631, 793, 800, 850, 875
Hpy8I GTNNAC 1 cut(s) 979
HpyAV CCTTC 4 cut(s) 233, 302, 471, 728
HpyCH4III ACNGT 2 cut(s) 331, 521
HpyCH4IV ACGT 3 cut(s) 15, 62, 80
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 26, 912
HpySE526I ACGT 3 cut(s) 15, 62, 80
Kpn2I TCCGGA 1 cut(s) 622
Kzo9I GATC 5 cut(s) 94, 251, 406, 659, 996
LmnI GCTCC 4 cut(s) 555, 602, 839, 914
Lsp1109I GCAGC 9 cut(s) 26, 60, 711, 818, 843, 868, 893, 919, 969
LweI GCATC 2 cut(s) 279, 557
MaeI CTAG 1 cut(s) 8
MaeII ACGT 3 cut(s) 15, 62, 80
MaeIII GTNAC 1 cut(s) 612
MalI GATC 5 cut(s) 96, 253, 408, 661, 998
MboI GATC 5 cut(s) 94, 251, 406, 659, 996
MboII GAAGA 8 cut(s) 182, 338, 401, 452, 492, 495, 554, 647
MfeI CAATTG 1 cut(s) 644
MhlI GDGCHC 2 cut(s) 62, 399
MlyI GAGTC 2 cut(s) 790, 1022
MnlI CCTC 9 cut(s) 84, 230, 258, 440, 529, 532, 768, 808, 1002
Mph1103I ATGCAT 2 cut(s) 27, 272
MroI TCCGGA 1 cut(s) 622
MseI TTAA 1 cut(s) 372
MspI CCGG 5 cut(s) 87, 623, 837, 886, 912
MspR9I CCNGG 3 cut(s) 87, 88, 886
MunI CAATTG 1 cut(s) 644
MwoI GCNNNNNNNGC 3 cut(s) 17, 26, 912
NciI CCSGG 3 cut(s) 87, 88, 886
NdeII GATC 5 cut(s) 94, 251, 406, 659, 996
NheI GCTAGC 1 cut(s) 7
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 612
NsiI ATGCAT 2 cut(s) 27, 272
NspI RCATGY 6 cut(s) 29, 46, 270, 893, 919, 945
PaeI GCATGC 2 cut(s) 29, 46
PciI ACATGT 1 cut(s) 941
PfeI GAWTC 3 cut(s) 238, 640, 868
PkrI GCNGC 9 cut(s) 41, 75, 726, 833, 858, 883, 908, 934, 959
Ple19I CGATCG 1 cut(s) 662
PleI GAGTC 2 cut(s) 790, 1021
PmaCI CACGTG 1 cut(s) 63
PmlI CACGTG 1 cut(s) 63
PpsI GAGTC 2 cut(s) 790, 1021
Ppu21I YACGTR 2 cut(s) 63, 81
PscI ACATGT 1 cut(s) 941
Psp124BI GAGCTC 1 cut(s) 399
PspCI CACGTG 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 860
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 5 cut(s) 199, 217, 311, 339, 817
PstI CTGCAG 1 cut(s) 790
PstNI CAGNNNCTG 2 cut(s) 605, 785
PvuI CGATCG 1 cut(s) 662
RsaI GTAC 3 cut(s) 83, 587, 598
RsaNI GTAC 3 cut(s) 82, 586, 597
SacI GAGCTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 9 cut(s) 40, 74, 725, 832, 857, 882, 907, 933, 958
Sau3AI GATC 5 cut(s) 94, 251, 406, 659, 996
Sau96I GGNCC 5 cut(s) 199, 217, 311, 339, 817
SchI GAGTC 2 cut(s) 790, 1022
ScrFI CCNGG 3 cut(s) 87, 88, 886
SduI GDGCHC 2 cut(s) 62, 399
SfaNI GCATC 2 cut(s) 279, 557
SfcI CTRYAG 1 cut(s) 786
SinI GGWCC 2 cut(s) 311, 817
SmaI CCCGGG 1 cut(s) 88
SnaBI TACGTA 1 cut(s) 81
SphI GCATGC 2 cut(s) 29, 46
SsiI CCGC 1 cut(s) 11
SspMI CTAG 1 cut(s) 8
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 3 cut(s) 85, 86, 884
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 331, 521
TaiI ACGT 3 cut(s) 18, 65, 83
TaqI TCGA 3 cut(s) 250, 421, 658
TatI WGTACW 1 cut(s) 596
TfiI GAWTC 3 cut(s) 238, 640, 868
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 9 cut(s) 39, 73, 724, 831, 856, 881, 906, 932, 957
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 4 cut(s) 835, 860, 884, 910
TspMI CCCGGG 1 cut(s) 86
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 311, 817
XapI RAATTY 5 cut(s) 186, 205, 618, 634, 743
XceI RCATGY 6 cut(s) 29, 46, 270, 893, 919, 945
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmaI CCCGGG 1 cut(s) 86
XspI CTAG 1 cut(s) 8
Zsp2I ATGCAT 2 cut(s) 27, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.