Rorug05G0260100

Cleavage site for pathogenic type III effector avirulence factor Avr

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001604
Physical Location & Seq
Reverse (-)
29309474 .. 29324726
15253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0260100.1

Sequence Viewer

Length: 2106 bp
ATGAAGAAAGATGACAGCTGCATCATGCAACTCATTAATGCCAAGGGCGAATTCAATGCTTCCGGGCTCGACAACTTTGTCAAGGAAGTGAAGCTTGATAGTTGCGGGGTCTTCTATAACATAGTTTCCATTATGGGCCCTCAAGGCGGCGGGAAGAGCACTTTACTGAACCAACTTTTCGGCACGAAGTTCAGGGTAATGGACTCACATAGGAGGACACAAACAACAAAGGGCGTTTGGATTGCCAAATGTGTTGGAATTAAGGATTGCACGGTTGCCATGGATTTGGAGGGTACCGATAGCGAGGAGAGAGGCCAGGATGATACTTCTTTCGAGAAACAAGTTGTCCTATTTGCGCTGGCAGTTTCTGACATTTTGCTCATAAATATGTGCTACAAAGAAATTGGACGGGAGCATGCAGCAAACAAACCTTTACTAAGAACAGTATTTCAAGTCATGATGCAGATAAACATCCCCAAACGTAAAAAGACGTTACTGTTTGTTATACGTGATGCTGATACATCAGAGAACCACCGAGTAGCCCTTAAAGATCAATTATTGGACAATATACAGAAGATATGGGCTGAAGTTCAGAAGCATGCGGGGACTCAACTCAATGACATTTTTGTGGTAGAAGTAGTTGCTTTGTCCCATTATCGATATGAAAAGGACACGTTTAACAAGGAGGTTGATGAACTGAGACTTGGAAAAGCGATGTCTATGGCTGGTAATAGAGAGGGTGTCATCCCTGGCGACGCATTCTCTTTCAGTTCACAGAAAATTTGGGAAGAAATAAAAACGAACAAGGAGCTGAATCTTCCTTCTATCAAGGTTCTGGCAGCCAACTTTATGTGTGAAAACATGGCCAAGAAAAAATTCAATCAGTTGATCTCTAGTAAGGATTGGTTGGACCTGGTAGAAGCTGTCAAAAATGGTCCTGTAGAAGGTTCGGGTGAACGTCTCAGCTCAATTCTAGACACCCATCTTTCTGGCTATGACGAGGAGGCCAAGTACTATGACAAAGGTGTGAGGGACTCAAAACGACATGACCTACTGGAGCAAACCGCATTGGATTTTGTGCTCCCGGCATACACTACCATGCTCCGCCGCCTGCATTCTAGAACACTTGAGAATTTTAAAGTAGAACTGAAGCAGTCATTAAACAATTCTGGAGAAGGATCTACTTCATCTGTTAGTACCTGCATCAACTCTTCCATGCAAAAGTTTGACCAAGGATGTAAAGAAACTGCCATGCAACGGGCCAATTGGGATTCTTCAAGCACTCGAAAAAACCTTCAGTTGGAAATTAATGAACATGTTGACTTTCTGAGAAGAGAGCTGATAATCAACTCTAAAGAACAACTTTCTGAATCTATATCTGCAAGTGCTCGGCGCCTACTTGAAGATGGCGGACAAGACACCTGGATCGAAATAAGAAAGCTTTTTAATCATGAGACTGAAGCTGCAGTAAAAAAATTATCAACTGCACTTGCTCGTTTTGAGTTGGACAAAGTAACAATTCACGATAAGGTGCAAGAATTGCGCGTGCATGCAAGAGAAGCGGTGGTGAAACAAGCAAAAAGAGAGGCTGTCGATGTCAAACTTCTGAGCGACATGAAAGAACAGTTTGAAAATGACTTCAAAAAACACAGCGACTCAAATCTTGAATTTTGTAAGGGTAAAGAAAACATTCGAAAAAATGCCATGAATGCCCGCAAAAATATTATTAGTCAGATTTTATCGGTTAGGGCTGCCATTCGCTTGGATGCTGAGAAGCCAGATAGTATTGAAAATGTTCTCATTTCTTCTCTAATGGGTGAAAATGGAGAGTCTACAAATTCACTTACCTCAAGTGAATGGTTGGAGTTTTCTTCGGACGATACAATAATTACTCTAATTAAACCACCGGACTGTAAGGAGTTGTGGAGGCGGTTCGAAGGAGAGACGGAGAAGATTGTCGAGCAAGCTACTTTAGCCTGGGAAGCCCACCGGAGGAGGAAGACTGCAAAAGTTGCTGCAACTGCTGGGAAAATTGTTCTTGAACTGACCAGCGCCGTGCTTCATGTGCTTGGGATCCATACATACATAAGTGACACGTACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

701

Amino Acids

79.35

Weight (kDa)

6.64

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
URGCP_GTPase PF25683 40 - 207 1.8e-10 URGCP-like GTPase domain
RHD3_GTPase PF05879 45 - 274 2.8e-76 Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB PF20428 318 - 664 1.1e-56 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 1208
Acc65I GGTACC 1 cut(s) 293
AccB1I GGYRCC 2 cut(s) 293, 1392
AccI GTMKAC 1 cut(s) 1832
AccII CGCG 1 cut(s) 1545
AclWI GGATC 4 cut(s) 1186, 1433, 2068, 2081
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 6 cut(s) 50, 780, 875, 1132, 1667, 1837
AcuI CTGAAG 4 cut(s) 606, 1169, 1280, 1479
AcyI GRCGYC 1 cut(s) 1393
AfaI GTAC 4 cut(s) 295, 1013, 1198, 2099
AfiI CCNNNNNNNGG 5 cut(s) 146, 944, 1257, 1300, 1992
AflIII ACRYGT 3 cut(s) 672, 1315, 2094
AjnI CCWGG 5 cut(s) 315, 748, 912, 1421, 1976
AjuI GAANNNNNNNTTGG 6 cut(s) 35, 67, 687, 719, 860, 892
AluBI AGCT 9 cut(s) 18, 94, 811, 923, 966, 1339, 1441, 1463, 1967
AluI AGCT 9 cut(s) 18, 94, 811, 923, 966, 1339, 1441, 1463, 1967
Alw21I GWGCWC 3 cut(s) 161, 1083, 1390
Alw26I GTCTC 4 cut(s) 694, 965, 1448, 1937
AlwI GGATC 4 cut(s) 1186, 1433, 2068, 2081
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 5 cut(s) 136, 313, 864, 1005, 1260
ApaI GGGCCC 1 cut(s) 140
ApeKI GCWGC 6 cut(s) 18, 419, 839, 1463, 1751, 2015
ApoI RAATTY 6 cut(s) 50, 780, 875, 1132, 1667, 1837
AseI ATTAAT 2 cut(s) 36, 1308
Asp700I GAANNNNTTC 3 cut(s) 875, 1689, 1794
Asp718I GGTACC 1 cut(s) 293
AspLEI GCGC 4 cut(s) 358, 1395, 1545, 2054
AspS9I GGNCC 5 cut(s) 136, 137, 910, 935, 1260
AsuC2I CCSGG 2 cut(s) 64, 1085
AsuHPI GGTGA 3 cut(s) 965, 1579, 1829
AsuII TTCGAA 2 cut(s) 1693, 1935
AvaII GGWCC 2 cut(s) 910, 935
BaeGI GKGCMC 1 cut(s) 140
BalI TGGCCA 1 cut(s) 866
BamHI GGATCC 1 cut(s) 2073
BanI GGYRCC 2 cut(s) 293, 1392
BanII GRGCYC 2 cut(s) 69, 140
BbsI GAAGAC 2 cut(s) 103, 2006
Bbv12I GWGCWC 3 cut(s) 161, 1083, 1390
BbvI GCAGC 6 cut(s) 5, 431, 851, 1450, 1738, 2002
BccI CCATC 2 cut(s) 990, 1400
BceAI ACGGC 1 cut(s) 2039
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 5 cut(s) 317, 750, 914, 1423, 1978
BcnI CCSGG 2 cut(s) 64, 1085
BcoDI GTCTC 4 cut(s) 694, 965, 1448, 1937
BfaI CTAG 4 cut(s) 894, 974, 1119, 2104
BfmI CTRYAG 2 cut(s) 939, 1464
BfoI RGCGCY 2 cut(s) 1396, 2055
BfuAI ACCTGC 1 cut(s) 1208
BglI GCCNNNNNGGC 1 cut(s) 144
BisI GCNGC 8 cut(s) 19, 148, 420, 840, 1108, 1464, 1752, 2016
BlsI GCNGC 8 cut(s) 20, 149, 421, 841, 1109, 1465, 1753, 2017
BmcAI AGTACT 1 cut(s) 1013
Bme1390I CCNGG 7 cut(s) 64, 317, 750, 914, 1085, 1423, 1978
Bme18I GGWCC 2 cut(s) 910, 935
BmgT120I GGNCC 5 cut(s) 136, 137, 910, 935, 1260
BmiI GGNNCC 4 cut(s) 138, 295, 1394, 2075
BmrFI CCNGG 7 cut(s) 64, 317, 750, 914, 1085, 1423, 1978
BmsI GCATC 5 cut(s) 30, 450, 502, 1212, 1756
BpiI GAAGAC 2 cut(s) 103, 2006
BpmI CTGGAG 2 cut(s) 1076, 1191
Bpu14I TTCGAA 2 cut(s) 1693, 1935
BpuEI CTTGAG 3 cut(s) 126, 1148, 1834
BpuMI CCSGG 2 cut(s) 64, 1085
Bsa29I ATCGAT 1 cut(s) 658
BsaAI YACGTR 2 cut(s) 509, 2097
BsaBI GATNNNNATC 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 1393
BsaJI CCNNGG 5 cut(s) 42, 279, 748, 1231, 1977
BsaWI WCCGGW 2 cut(s) 1906, 1989
BsaXI ACNNNNNCTCC 2 cut(s) 995, 1025
Bsc4I CCNNNNNNNGG 5 cut(s) 146, 944, 1257, 1300, 1992
Bse1I ACTGG 1 cut(s) 1059
Bse8I GATNNNNATC 1 cut(s) 470
BseBI CCWGG 5 cut(s) 317, 750, 914, 1423, 1978
BseCI ATCGAT 1 cut(s) 658
BseDI CCNNGG 5 cut(s) 42, 279, 748, 1231, 1977
BseGI GGATG 5 cut(s) 325, 471, 744, 1241, 1771
BseJI GATNNNNATC 1 cut(s) 470
BseLI CCNNNNNNNGG 5 cut(s) 146, 944, 1257, 1300, 1992
BseMII CTCAG 5 cut(s) 689, 976, 1319, 1598, 1761
BseNI ACTGG 1 cut(s) 1059
BseRI GAGGAG 3 cut(s) 320, 1016, 2008
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 6 cut(s) 5, 431, 851, 1450, 1738, 2002
BseYI CCCAGC 1 cut(s) 2024
BsgI GTGCAG 1 cut(s) 1470
Bsh1236I CGCG 1 cut(s) 1545
BshFI GGCC 5 cut(s) 138, 315, 866, 1007, 1262
BshNI GGYRCC 2 cut(s) 293, 1392
BshVI ATCGAT 1 cut(s) 658
BsiHKAI GWGCWC 3 cut(s) 161, 1083, 1390
BsiSI CCGG 4 cut(s) 63, 1085, 1907, 1990
BslFI GGGAC 3 cut(s) 619, 634, 1046
BslI CCNNNNNNNGG 5 cut(s) 146, 944, 1257, 1300, 1992
BsmAI GTCTC 4 cut(s) 694, 965, 1448, 1937
BsmBI CGTCTC 2 cut(s) 965, 1937
BsmFI GGGAC 3 cut(s) 619, 634, 1046
BsmI GAATGC 3 cut(s) 758, 1114, 1714
BsnI GGCC 5 cut(s) 138, 315, 866, 1007, 1262
Bsp119I TTCGAA 2 cut(s) 1693, 1935
Bsp120I GGGCCC 1 cut(s) 136
Bsp1286I GDGCHC 5 cut(s) 69, 140, 161, 1083, 1390
Bsp143I GATC 5 cut(s) 550, 888, 1178, 1425, 2073
Bsp19I CCATGG 1 cut(s) 279
BspANI GGCC 5 cut(s) 138, 315, 866, 1007, 1262
BspCNI CTCAG 5 cut(s) 690, 975, 1320, 1599, 1762
BspDI ATCGAT 1 cut(s) 658
BspFNI CGCG 1 cut(s) 1545
BspHI TCATGA 2 cut(s) 456, 1450
BspLI GGNNCC 4 cut(s) 138, 295, 1394, 2075
BspMAI CTGCAG 1 cut(s) 1468
BspMI ACCTGC 1 cut(s) 1208
BspPI GGATC 4 cut(s) 1186, 1433, 2068, 2081
BspQI GCTCTTC 1 cut(s) 149
BspT104I TTCGAA 2 cut(s) 1693, 1935
BspT107I GGYRCC 2 cut(s) 293, 1392
BsrI ACTGG 1 cut(s) 1059
BssECI CCNNGG 5 cut(s) 42, 279, 748, 1231, 1977
BssMI GATC 5 cut(s) 550, 888, 1178, 1425, 2073
BssNI GRCGYC 1 cut(s) 1393
BssT1I CCWWGG 3 cut(s) 42, 279, 1231
Bst2UI CCWGG 5 cut(s) 317, 750, 914, 1423, 1978
Bst4CI ACNGT 5 cut(s) 274, 445, 498, 1626, 1913
Bst6I CTCTTC 3 cut(s) 149, 1216, 1327
BstACI GRCGYC 1 cut(s) 1393
BstAPI GCANNNNNTGC 2 cut(s) 1540, 2012
BstBAI YACGTR 2 cut(s) 509, 2097
BstBI TTCGAA 2 cut(s) 1693, 1935
BstC8I GCNNGC 8 cut(s) 360, 417, 600, 1112, 1547, 1551, 1714, 1965
BstDEI CTNAG 6 cut(s) 437, 698, 962, 1328, 1607, 1770
BstDSI CCRYGG 1 cut(s) 279
BstENI CCTNNNNNAGG 1 cut(s) 942
BstF5I GGATG 5 cut(s) 325, 471, 744, 1241, 1771
BstFNI CGCG 1 cut(s) 1545
BstH2I RGCGCY 2 cut(s) 1396, 2055
BstHHI GCGC 4 cut(s) 358, 1395, 1545, 2054
BstKTI GATC 5 cut(s) 553, 891, 1181, 1428, 2076
BstMAI GTCTC 4 cut(s) 694, 965, 1448, 1937
BstMBI GATC 5 cut(s) 550, 888, 1178, 1425, 2073
BstNI CCWGG 5 cut(s) 317, 750, 914, 1423, 1978
BstNSI RCATGY 4 cut(s) 419, 602, 1319, 1553
BstSCI CCNGG 7 cut(s) 62, 315, 748, 912, 1083, 1421, 1976
BstSFI CTRYAG 2 cut(s) 939, 1464
BstSLI GKGCMC 1 cut(s) 140
BstUI CGCG 1 cut(s) 1545
BstV1I GCAGC 6 cut(s) 5, 431, 851, 1450, 1738, 2002
BstV2I GAAGAC 2 cut(s) 103, 2006
BstX2I RGATCY 2 cut(s) 1178, 2073
BstXI CCANNNNNNTGG 3 cut(s) 286, 989, 1762
BstYI RGATCY 2 cut(s) 1178, 2073
Bsu15I ATCGAT 1 cut(s) 658
BsuRI GGCC 5 cut(s) 138, 315, 866, 1007, 1262
BsuTUI ATCGAT 1 cut(s) 658
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 728
BtsCI GGATG 5 cut(s) 325, 471, 744, 1241, 1771
BveI ACCTGC 1 cut(s) 1208
Cac8I GCNNGC 8 cut(s) 360, 417, 600, 1112, 1547, 1551, 1714, 1965
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 2 cut(s) 456, 1450
CfoI GCGC 4 cut(s) 358, 1395, 1545, 2054
Cfr13I GGNCC 5 cut(s) 136, 137, 910, 935, 1260
ClaI ATCGAT 1 cut(s) 658
CseI GACGC 1 cut(s) 764
CsiI ACCWGGT 1 cut(s) 912
Csp6I GTAC 4 cut(s) 294, 1012, 1197, 2098
CviQI GTAC 4 cut(s) 294, 1012, 1197, 2098
DdeI CTNAG 6 cut(s) 437, 698, 962, 1328, 1607, 1770
DinI GGCGCC 1 cut(s) 1394
DpnI GATC 5 cut(s) 552, 890, 1180, 1427, 2075
DpnII GATC 5 cut(s) 550, 888, 1178, 1425, 2073
DraI TTTAAA 1 cut(s) 1138
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 3 cut(s) 149, 1216, 1327
EarI CTCTTC 3 cut(s) 149, 1216, 1327
EciI GGCGGA 2 cut(s) 1094, 1425
Eco130I CCWWGG 3 cut(s) 42, 279, 1231
Eco24I GRGCYC 2 cut(s) 69, 140
Eco47I GGWCC 2 cut(s) 910, 935
Eco57I CTGAAG 4 cut(s) 606, 1169, 1280, 1479
EcoNI CCTNNNNNAGG 1 cut(s) 942
EcoO109I RGGNCCY 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 5 cut(s) 315, 748, 912, 1421, 1976
EcoT14I CCWWGG 3 cut(s) 42, 279, 1231
EcoT38I GRGCYC 2 cut(s) 69, 140
EgeI GGCGCC 1 cut(s) 1394
EheI GGCGCC 1 cut(s) 1394
ErhI CCWWGG 3 cut(s) 42, 279, 1231
Esp3I CGTCTC 2 cut(s) 965, 1937
FalI AAGNNNNNCTT 4 cut(s) 78, 110, 1347, 1379
FaqI GGGAC 3 cut(s) 619, 634, 1046
FauI CCCGC 4 cut(s) 98, 143, 595, 1721
FblI GTMKAC 1 cut(s) 1832
Fnu4HI GCNGC 8 cut(s) 19, 148, 420, 840, 1108, 1464, 1752, 2016
FokI GGATG 5 cut(s) 332, 458, 731, 1248, 1778
FriOI GRGCYC 2 cut(s) 69, 140
Fsp4HI GCNGC 8 cut(s) 19, 148, 420, 840, 1108, 1464, 1752, 2016
FspBI CTAG 4 cut(s) 894, 974, 1119, 2104
GlaI GCGC 4 cut(s) 357, 1394, 1544, 2053
GluI GCNGC 8 cut(s) 19, 148, 420, 840, 1108, 1464, 1752, 2016
GsaI CCCAGC 1 cut(s) 2028
GsuI CTGGAG 2 cut(s) 1076, 1191
HaeII RGCGCY 2 cut(s) 1396, 2055
HaeIII GGCC 5 cut(s) 138, 315, 866, 1007, 1262
HapII CCGG 4 cut(s) 63, 1085, 1907, 1990
HgaI GACGC 1 cut(s) 764
HhaI GCGC 4 cut(s) 358, 1395, 1545, 2054
Hin1I GRCGYC 1 cut(s) 1393
Hin6I GCGC 4 cut(s) 356, 1393, 1543, 2052
HinP1I GCGC 4 cut(s) 356, 1393, 1543, 2052
HincII GTYRAC 1 cut(s) 1321
HindII GTYRAC 1 cut(s) 1321
HindIII AAGCTT 2 cut(s) 92, 1439
HinfI GANTC 8 cut(s) 203, 607, 814, 1034, 1271, 1370, 1655, 1829
HpaII CCGG 4 cut(s) 63, 1085, 1907, 1990
HphI GGTGA 3 cut(s) 965, 1579, 1829
Hpy166II GTNNAC 4 cut(s) 773, 956, 1321, 1833
Hpy188I TCNGA 8 cut(s) 370, 526, 594, 1329, 1369, 1608, 1734, 1876
Hpy188III TCNNGA 9 cut(s) 334, 457, 974, 1119, 1170, 1451, 1523, 1664, 2039
Hpy8I GTNNAC 4 cut(s) 773, 956, 1321, 1833
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 5 cut(s) 831, 938, 1169, 1304, 1931
HpyCH4III ACNGT 5 cut(s) 274, 445, 498, 1626, 1913
HpyCH4IV ACGT 6 cut(s) 481, 491, 508, 674, 958, 2096
HpyF3I CTNAG 6 cut(s) 437, 698, 962, 1328, 1607, 1770
HpySE526I ACGT 6 cut(s) 481, 491, 508, 674, 958, 2096
Hsp92I GRCGYC 1 cut(s) 1393
HspAI GCGC 4 cut(s) 356, 1393, 1543, 2052
KasI GGCGCC 1 cut(s) 1392
KpnI GGTACC 1 cut(s) 297
Kzo9I GATC 5 cut(s) 550, 888, 1178, 1425, 2073
LguI GCTCTTC 1 cut(s) 149
LmnI GCTCC 5 cut(s) 412, 808, 1057, 1086, 1107
Lsp1109I GCAGC 6 cut(s) 5, 431, 851, 1450, 1738, 2002
LweI GCATC 5 cut(s) 30, 450, 502, 1212, 1756
MabI ACCWGGT 1 cut(s) 912
MaeI CTAG 4 cut(s) 894, 974, 1119, 2104
MaeII ACGT 6 cut(s) 481, 491, 508, 674, 958, 2096
MaeIII GTNAC 3 cut(s) 492, 1513, 2090
MalI GATC 5 cut(s) 552, 890, 1180, 1427, 2075
MboI GATC 5 cut(s) 550, 888, 1178, 1425, 2073
MfeI CAATTG 1 cut(s) 1264
MflI RGATCY 2 cut(s) 1178, 2073
MhlI GDGCHC 5 cut(s) 69, 140, 161, 1083, 1390
MlsI TGGCCA 1 cut(s) 866
MluNI TGGCCA 1 cut(s) 866
Mly113I GGCGCC 1 cut(s) 1393
MlyI GAGTC 5 cut(s) 197, 601, 1028, 1649, 1838
MmeI TCCRAC 5 cut(s) 235, 888, 1281, 1485, 1842
Mox20I TGGCCA 1 cut(s) 866
MroXI GAANNNNTTC 3 cut(s) 875, 1689, 1794
MscI TGGCCA 1 cut(s) 866
MseI TTAA 9 cut(s) 36, 261, 546, 678, 1137, 1160, 1308, 1446, 1899
MslI CAYNNNNRTG 3 cut(s) 386, 626, 1097
Msp20I TGGCCA 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 18
MspI CCGG 4 cut(s) 63, 1085, 1907, 1990
MspR9I CCNGG 7 cut(s) 64, 317, 750, 914, 1085, 1423, 1978
MunI CAATTG 1 cut(s) 1264
Mva1269I GAATGC 3 cut(s) 758, 1114, 1714
MvaI CCWGG 5 cut(s) 317, 750, 914, 1423, 1978
MvnI CGCG 1 cut(s) 1545
NarI GGCGCC 1 cut(s) 1393
NciI CCSGG 2 cut(s) 64, 1085
NcoI CCATGG 1 cut(s) 279
NdeII GATC 5 cut(s) 550, 888, 1178, 1425, 2073
NlaIV GGNNCC 4 cut(s) 138, 295, 1394, 2075
NmeAIII GCCGAG 1 cut(s) 1369
NmuCI GTSAC 1 cut(s) 2090
NspI RCATGY 4 cut(s) 419, 602, 1319, 1553
NspV TTCGAA 2 cut(s) 1693, 1935
PaeI GCATGC 3 cut(s) 419, 602, 1553
PagI TCATGA 2 cut(s) 456, 1450
PciI ACATGT 1 cut(s) 1315
PciSI GCTCTTC 1 cut(s) 149
PctI GAATGC 3 cut(s) 758, 1114, 1714
PdmI GAANNNNTTC 3 cut(s) 875, 1689, 1794
PfeI GAWTC 3 cut(s) 814, 1271, 1370
PkrI GCNGC 8 cut(s) 20, 149, 421, 841, 1109, 1465, 1753, 2017
PleI GAGTC 5 cut(s) 197, 601, 1028, 1649, 1837
PluTI GGCGCC 1 cut(s) 1396
PpsI GAGTC 5 cut(s) 197, 601, 1028, 1649, 1837
Ppu21I YACGTR 2 cut(s) 509, 2097
PscI ACATGT 1 cut(s) 1315
PshBI ATTAAT 2 cut(s) 36, 1308
Psp6I CCWGG 5 cut(s) 315, 748, 912, 1421, 1976
PspFI CCCAGC 1 cut(s) 2024
PspGI CCWGG 5 cut(s) 315, 748, 912, 1421, 1976
PspN4I GGNNCC 4 cut(s) 138, 295, 1394, 2075
PspOMI GGGCCC 1 cut(s) 136
PspPI GGNCC 5 cut(s) 136, 137, 910, 935, 1260
PstI CTGCAG 1 cut(s) 1468
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 2 cut(s) 1178, 2073
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 4 cut(s) 295, 1013, 1198, 2099
RsaNI GTAC 4 cut(s) 294, 1012, 1197, 2098
RseI CAYNNNNRTG 3 cut(s) 386, 626, 1097
SapI GCTCTTC 1 cut(s) 149
SaqAI TTAA 9 cut(s) 36, 261, 546, 678, 1137, 1160, 1308, 1446, 1899
SatI GCNGC 8 cut(s) 19, 148, 420, 840, 1108, 1464, 1752, 2016
Sau3AI GATC 5 cut(s) 550, 888, 1178, 1425, 2073
Sau96I GGNCC 5 cut(s) 136, 137, 910, 935, 1260
ScaI AGTACT 1 cut(s) 1013
SchI GAGTC 5 cut(s) 197, 601, 1028, 1649, 1838
ScrFI CCNGG 7 cut(s) 64, 317, 750, 914, 1085, 1423, 1978
SduI GDGCHC 5 cut(s) 69, 140, 161, 1083, 1390
SexAI ACCWGGT 1 cut(s) 912
SfaNI GCATC 5 cut(s) 30, 450, 502, 1212, 1756
SfcI CTRYAG 2 cut(s) 939, 1464
SfoI GGCGCC 1 cut(s) 1394
SfuI TTCGAA 2 cut(s) 1693, 1935
SinI GGWCC 2 cut(s) 910, 935
SmiMI CAYNNNNRTG 3 cut(s) 386, 626, 1097
SmlI CTYRAG 3 cut(s) 141, 1127, 1849
SmoI CTYRAG 3 cut(s) 141, 1127, 1849
SphI GCATGC 3 cut(s) 419, 602, 1553
SspDI GGCGCC 1 cut(s) 1392
SspI AATATT 1 cut(s) 1723
SspMI CTAG 4 cut(s) 894, 974, 1119, 2104
StyD4I CCNGG 7 cut(s) 62, 315, 748, 912, 1083, 1421, 1976
StyI CCWWGG 3 cut(s) 42, 279, 1231
TaaI ACNGT 5 cut(s) 274, 445, 498, 1626, 1913
TaiI ACGT 6 cut(s) 484, 494, 511, 677, 961, 2099
TaqI TCGA 9 cut(s) 69, 333, 658, 1285, 1428, 1593, 1693, 1935, 1959
TatI WGTACW 1 cut(s) 1011
TauI GCSGC 2 cut(s) 150, 1110
TfiI GAWTC 3 cut(s) 814, 1271, 1370
Tru1I TTAA 9 cut(s) 36, 261, 546, 678, 1137, 1160, 1308, 1446, 1899
Tru9I TTAA 9 cut(s) 36, 261, 546, 678, 1137, 1160, 1308, 1446, 1899
TseFI GTSAC 1 cut(s) 2090
TseI GCWGC 6 cut(s) 18, 419, 839, 1463, 1751, 2015
Tsp45I GTSAC 1 cut(s) 2090
TspDTI ATGAA 8 cut(s) 17, 678, 708, 1176, 1326, 1631, 1721, 2051
TspGWI ACGGA 1 cut(s) 1961
VpaK11BI GGWCC 2 cut(s) 910, 935
VspI ATTAAT 2 cut(s) 36, 1308
XagI CCTNNNNNAGG 1 cut(s) 942
XapI RAATTY 6 cut(s) 50, 780, 875, 1132, 1667, 1837
XbaI TCTAGA 2 cut(s) 973, 1118
XceI RCATGY 4 cut(s) 419, 602, 1319, 1553
XmiI GTMKAC 1 cut(s) 1832
XmnI GAANNNNTTC 3 cut(s) 875, 1689, 1794
XspI CTAG 4 cut(s) 894, 974, 1119, 2104
ZrmI AGTACT 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.