FvH4_6g42700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001604
Physical Location & Seq
Forward (+)
33213820 .. 33215339
1520 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42700.t1

Sequence Viewer

Length: 951 bp
ATGGATTCCATGACACTTCCTCAAGAGCTTCCCACCATCAATTTCTCCCTCCAAGACTTGAAGCCTGGCTCAAGCTCCTGGACTTCCACCTGCAAACAAGTCCGCAATGCACTCGAAGAATACGGTTGCTTTGTGGCATTGTACCCACAAGTCTCCCAAGAGCTCATGGACAATATCTTCGGCCAATCCAGGGATCTGTTCGAGGTTCCCCTCGAGAACAAGGTCAAGAACACCAGCGAGGAGCCTTACCGTGGCTATATCGGACCAAACCCCCTCTTGCCGCTCTATGAAGGCATTGCCATCGACAACGTCACATCCCCACAAGAAACTCAGAAGTTCAGGGACCTCATGTGGCCTAATGGAAAGACCCAATTCTGTGAAATCACAGATCTGTTTGCCAAGTTGCTCGGGGAGTTGGAGAACACTGTGGAAAAGATGATGTTCGAAAGCTACGGGATACCTCTGGAGCAATATGAAACGTTGGCGAGTTCCAACAGTCATCTTCTTCGTTTTCTCAAGTACAAGATACCAGAAGAGAGAGACGCGGCCCTTAGGTTTCCGAGCCACACAGACAAGAACTTCACCACCATTATCGTTCAGCATGATGTCGGTGGCCTCGAGGTTCAGACTAAGGATGGTTATTGGATCAGTGTCGAGTCTGCACCTTCTCAGTTCCTCTTCATGGCCTGTGATGGACTTCAGGTATGGAGCAATGACAGAGTGAAAGCTCCCCACCATCGTGTGAAGCACTGCGGGGACAAGACTCGATATTCACTGGGAATGTTTACGTTCAATAATGGAGTATTTGAAGTACCCAAAGAGCTAGTAGACGACAGCCACCCACTCCTCTATAACCAGTTCGACAGCCTTGGCTTCGTAAGGTTTTACACCACACCAGAGGCCAAGAAGGCAGAGTCACCCATCAAAGCCTACTGCGGTGTTAAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

317

Amino Acids

36.09

Weight (kDa)

5.19

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 10 - 89 1.7e-13 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 171 - 263 4.9e-19 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 98
AbsI CCTCGAGG 1 cut(s) 617
Acc36I ACCTGC 1 cut(s) 98
AccBSI CCGCTC 1 cut(s) 283
AccI GTMKAC 1 cut(s) 828
AccII CGCG 1 cut(s) 545
AciI CCGC 5 cut(s) 103, 281, 545, 753, 936
AclI AACGTT 1 cut(s) 479
AclWI GGATC 2 cut(s) 201, 653
AcoI YGGCCR 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 683
AfaI GTAC 3 cut(s) 143, 521, 813
AfiI CCNNNNNNNGG 3 cut(s) 190, 251, 682
AgsI TTSAA 3 cut(s) 61, 793, 809
AjnI CCWGG 3 cut(s) 64, 77, 188
AleI CACNNNNGTG 1 cut(s) 738
AluBI AGCT 6 cut(s) 28, 75, 163, 450, 728, 823
AluI AGCT 6 cut(s) 28, 75, 163, 450, 728, 823
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 2 cut(s) 157, 534
AlwI GGATC 2 cut(s) 201, 653
Ama87I CYCGRG 3 cut(s) 212, 407, 617
AoxI GGCC 6 cut(s) 181, 353, 546, 613, 684, 900
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspS9I GGNCC 3 cut(s) 263, 343, 547
AsuHPI GGTGA 2 cut(s) 574, 909
AsuII TTCGAA 1 cut(s) 444
AvaI CYCGRG 3 cut(s) 212, 407, 617
AvaII GGWCC 2 cut(s) 263, 343
AxyI CCTNAGG 1 cut(s) 551
BanII GRGCYC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 6 cut(s) 44, 308, 629, 686, 744, 929
BcgI CGANNNNNNTGC 4 cut(s) 94, 128, 283, 317
BciT130I CCWGG 3 cut(s) 66, 79, 190
BciVI GTATCC 1 cut(s) 450
BcoDI GTCTC 2 cut(s) 157, 534
BfaI CTAG 2 cut(s) 824, 949
BfuAI ACCTGC 1 cut(s) 98
BfuI GTATCC 1 cut(s) 450
BglI GCCNNNNNGGC 1 cut(s) 908
BglII AGATCT 1 cut(s) 388
BisI GCNGC 2 cut(s) 281, 546
BlsI GCNGC 2 cut(s) 282, 547
Bme1390I CCNGG 3 cut(s) 66, 79, 190
Bme18I GGWCC 2 cut(s) 263, 343
BmeT110I CYCGRG 3 cut(s) 212, 407, 617
BmgT120I GGNCC 3 cut(s) 263, 343, 547
BmiI GGNNCC 3 cut(s) 207, 243, 344
BmrFI CCNGG 3 cut(s) 66, 79, 190
BmrI ACTGGG 1 cut(s) 785
BmuI ACTGGG 1 cut(s) 785
BpmI CTGGAG 1 cut(s) 485
Bpu14I TTCGAA 1 cut(s) 444
BpuEI CTTGAG 3 cut(s) 6, 55, 500
BsaJI CCNNGG 3 cut(s) 189, 250, 868
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 251, 682
Bse1I ACTGG 2 cut(s) 780, 856
Bse21I CCTNAGG 1 cut(s) 551
Bse3DI GCAATG 3 cut(s) 112, 294, 718
BseBI CCWGG 3 cut(s) 66, 79, 190
BseDI CCNNGG 3 cut(s) 189, 250, 868
BseGI GGATG 2 cut(s) 314, 640
BseLI CCNNNNNNNGG 3 cut(s) 190, 251, 682
BseMI GCAATG 3 cut(s) 112, 294, 718
BseMII CTCAG 2 cut(s) 344, 683
BseNI ACTGG 2 cut(s) 780, 856
BseRI GAGGAG 2 cut(s) 254, 836
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 1 cut(s) 545
BshFI GGCC 6 cut(s) 183, 355, 548, 615, 686, 902
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 3 cut(s) 212, 407, 617
BslFI GGGAC 2 cut(s) 356, 770
BslI CCNNNNNNNGG 3 cut(s) 190, 251, 682
BsmAI GTCTC 2 cut(s) 157, 534
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 2 cut(s) 356, 770
BsnI GGCC 6 cut(s) 183, 355, 548, 615, 686, 902
BsoBI CYCGRG 3 cut(s) 212, 407, 617
Bsp119I TTCGAA 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 3 cut(s) 193, 388, 645
BspACI CCGC 5 cut(s) 103, 281, 545, 753, 936
BspANI GGCC 6 cut(s) 183, 355, 548, 615, 686, 902
BspCNI CTCAG 2 cut(s) 343, 682
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 3 cut(s) 207, 243, 344
BspMI ACCTGC 1 cut(s) 98
BspPI GGATC 2 cut(s) 201, 653
BspT104I TTCGAA 1 cut(s) 444
BsrBI CCGCTC 1 cut(s) 283
BsrDI GCAATG 3 cut(s) 112, 294, 718
BsrI ACTGG 2 cut(s) 780, 856
BssECI CCNNGG 3 cut(s) 189, 250, 868
BssMI GATC 3 cut(s) 193, 388, 645
BssT1I CCWWGG 1 cut(s) 868
Bst2UI CCWGG 3 cut(s) 66, 79, 190
Bst4CI ACNGT 4 cut(s) 125, 251, 427, 497
Bst6I CTCTTC 2 cut(s) 528, 683
BstBI TTCGAA 1 cut(s) 444
BstDEI CTNAG 4 cut(s) 330, 551, 630, 669
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 2 cut(s) 314, 640
BstFNI CGCG 1 cut(s) 545
BstKTI GATC 3 cut(s) 196, 391, 648
BstMAI GTCTC 2 cut(s) 157, 534
BstMBI GATC 3 cut(s) 193, 388, 645
BstMWI GCNNNNNNNGC 1 cut(s) 908
BstNI CCWGG 3 cut(s) 66, 79, 190
BstSCI CCNGG 3 cut(s) 64, 77, 188
BstUI CGCG 1 cut(s) 545
BstX2I RGATCY 2 cut(s) 193, 388
BstYI RGATCY 2 cut(s) 193, 388
Bsu36I CCTNAGG 1 cut(s) 551
BsuI GTATCC 1 cut(s) 450
BsuRI GGCC 6 cut(s) 183, 355, 548, 615, 686, 902
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 314, 640
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 4 cut(s) 423, 655, 748, 773
BveI ACCTGC 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 263, 343, 547
CseI GACGC 1 cut(s) 551
Csp6I GTAC 3 cut(s) 142, 520, 812
CviAII CATG 5 cut(s) 10, 166, 349, 602, 682
CviQI GTAC 3 cut(s) 142, 520, 812
DdeI CTNAG 4 cut(s) 330, 551, 630, 669
DpnI GATC 3 cut(s) 195, 390, 647
DpnII GATC 3 cut(s) 193, 388, 645
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 2 cut(s) 528, 683
EarI CTCTTC 2 cut(s) 528, 683
Ecl136II GAGCTC 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 868
Eco24I GRGCYC 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 263, 343
Eco53kI GAGCTC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 683
Eco81I CCTNAGG 1 cut(s) 551
Eco88I CYCGRG 3 cut(s) 212, 407, 617
EcoICRI GAGCTC 1 cut(s) 163
EcoO109I RGGNCCY 1 cut(s) 343
EcoRII CCWGG 3 cut(s) 64, 77, 188
EcoT14I CCWWGG 1 cut(s) 868
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 868
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 5 cut(s) 13, 169, 352, 605, 685
FaqI GGGAC 2 cut(s) 356, 770
FatI CATG 5 cut(s) 9, 165, 348, 601, 681
FauI CCCGC 1 cut(s) 746
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 2 cut(s) 281, 546
FokI GGATG 2 cut(s) 301, 647
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 2 cut(s) 281, 546
FspBI CTAG 2 cut(s) 824, 949
GluI GCNGC 2 cut(s) 281, 546
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 6 cut(s) 183, 355, 548, 615, 686, 902
HgaI GACGC 1 cut(s) 551
Hin1II CATG 5 cut(s) 13, 169, 352, 605, 685
HinfI GANTC 4 cut(s) 5, 656, 763, 914
HphI GGTGA 2 cut(s) 574, 909
Hpy166II GTNNAC 2 cut(s) 786, 829
Hpy188I TCNGA 4 cut(s) 263, 333, 561, 627
Hpy188III TCNNGA 4 cut(s) 23, 214, 226, 464
Hpy8I GTNNAC 2 cut(s) 786, 829
HpyAV CCTTC 3 cut(s) 284, 675, 901
HpyCH4III ACNGT 4 cut(s) 125, 251, 427, 497
HpyCH4IV ACGT 3 cut(s) 309, 479, 788
HpyCH4V TGCA 3 cut(s) 93, 110, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 908
HpyF3I CTNAG 4 cut(s) 330, 551, 630, 669
HpySE526I ACGT 3 cut(s) 309, 479, 788
Hsp92II CATG 5 cut(s) 13, 169, 352, 605, 685
Kzo9I GATC 3 cut(s) 193, 388, 645
LmnI GCTCC 5 cut(s) 80, 241, 466, 708, 733
MaeI CTAG 2 cut(s) 824, 949
MaeII ACGT 3 cut(s) 309, 479, 788
MaeIII GTNAC 2 cut(s) 310, 915
MalI GATC 3 cut(s) 195, 390, 647
MbiI CCGCTC 1 cut(s) 283
MboI GATC 3 cut(s) 193, 388, 645
MboII GAAGA 6 cut(s) 128, 169, 494, 497, 545, 670
MflI RGATCY 2 cut(s) 193, 388
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 2 cut(s) 40, 371
MlyI GAGTC 3 cut(s) 665, 757, 923
MmeI TCCRAC 2 cut(s) 396, 516
MseI TTAA 1 cut(s) 942
MslI CAYNNNNRTG 1 cut(s) 738
MspR9I CCNGG 3 cut(s) 66, 79, 190
MvaI CCWGG 3 cut(s) 66, 79, 190
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 908
NdeII GATC 3 cut(s) 193, 388, 645
NlaIII CATG 5 cut(s) 13, 169, 352, 605, 685
NlaIV GGNNCC 3 cut(s) 207, 243, 344
NmuCI GTSAC 2 cut(s) 310, 915
NspV TTCGAA 1 cut(s) 444
OliI CACNNNNGTG 1 cut(s) 738
PaeR7I CTCGAG 2 cut(s) 212, 617
PaqCI CACCTGC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 308
PfoI TCCNGGA 1 cut(s) 77
PkrI GCNGC 2 cut(s) 282, 547
PleI GAGTC 3 cut(s) 664, 757, 922
PpsI GAGTC 3 cut(s) 664, 757, 922
PpuMI RGGWCCY 1 cut(s) 343
Psp124BI GAGCTC 1 cut(s) 165
Psp1406I AACGTT 1 cut(s) 479
Psp5II RGGWCCY 1 cut(s) 343
Psp6I CCWGG 3 cut(s) 64, 77, 188
PspGI CCWGG 3 cut(s) 64, 77, 188
PspN4I GGNNCC 3 cut(s) 207, 243, 344
PspPI GGNCC 3 cut(s) 263, 343, 547
PspPPI RGGWCCY 1 cut(s) 343
PspXI VCTCGAGB 1 cut(s) 617
PsuI RGATCY 2 cut(s) 193, 388
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 3 cut(s) 143, 521, 813
RsaNI GTAC 3 cut(s) 142, 520, 812
RseI CAYNNNNRTG 1 cut(s) 738
SacI GAGCTC 1 cut(s) 165
SaqAI TTAA 1 cut(s) 942
SatI GCNGC 2 cut(s) 281, 546
Sau3AI GATC 3 cut(s) 193, 388, 645
Sau96I GGNCC 3 cut(s) 263, 343, 547
SchI GAGTC 3 cut(s) 665, 757, 923
ScrFI CCNGG 3 cut(s) 66, 79, 190
SduI GDGCHC 1 cut(s) 165
Sfr274I CTCGAG 2 cut(s) 212, 617
SfuI TTCGAA 1 cut(s) 444
SinI GGWCC 2 cut(s) 263, 343
SlaI CTCGAG 2 cut(s) 212, 617
SmiMI CAYNNNNRTG 1 cut(s) 738
SmlI CTYRAG 5 cut(s) 21, 70, 212, 515, 617
SmoI CTYRAG 5 cut(s) 21, 70, 212, 515, 617
Sse9I AATT 2 cut(s) 40, 371
SsiI CCGC 5 cut(s) 103, 281, 545, 753, 936
SspMI CTAG 2 cut(s) 824, 949
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 3 cut(s) 64, 77, 188
StyI CCWWGG 1 cut(s) 868
TaaI ACNGT 4 cut(s) 125, 251, 427, 497
TaiI ACGT 3 cut(s) 312, 482, 791
TaqI TCGA 9 cut(s) 114, 201, 213, 303, 444, 618, 654, 766, 861
TasI AATT 2 cut(s) 40, 371
TatI WGTACW 1 cut(s) 519
TauI GCSGC 2 cut(s) 283, 548
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 942
Tru9I TTAA 1 cut(s) 942
TscAI CASTG 4 cut(s) 430, 655, 755, 780
TseFI GTSAC 2 cut(s) 310, 915
Tsp45I GTSAC 2 cut(s) 310, 915
TspDTI ATGAA 3 cut(s) 303, 489, 670
TspRI CASTG 4 cut(s) 430, 655, 755, 780
Tth111I GACNNNGTC 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 263, 343
XhoI CTCGAG 2 cut(s) 212, 617
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 2 cut(s) 824, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.