FvH4_3g27760

Cleavage site for pathogenic type III effector avirulence factor Avr

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012034
Physical Location & Seq
Forward (+)
20796620 .. 20798630
2011 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27760.t1

Sequence Viewer

Length: 321 bp
ATGGGACGTCCGGAGGAGGGAAATGCAAATGGTCGGAAGTCAGTTCCTCAGTTTGGAGGATGGGAACAAAGCGCCACAGTAGAGCCTACGAGCTATACTATGGTGTTTAATAATGCTCGTGCTAATCGGAAGCAGAATAAGACAGACTTGACGGAATTCAAGCGCAATAGTCTTGGAAATGAACATGAATTAATGGCTACTCAAGGCCATCATGATCGTCATCATCATCATTCTGATGACTCTGTCACGAGGAAAAAGTCCTGGAATCCATTTAATTATTTTTTGTCTCTCTTTTCATGCAGAGGAAAAGGACAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

107

Amino Acids

12.21

Weight (kDa)

9.51

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 11 - 44 2.2e-10 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19473 AT5G19473
fragaria_vesca FvH4_3g27760 FvH4_3g27760
malus_domestica MD03G1164700.v1.1
prunus_persica Prupe.4G264200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0050361
rosa_laevigata RLG00000034699
rosa_multiflora Rmu_sc0005060.1_g000015
rosa_roxburghii Rroxscaffold_1G00030580
rosa_rugosa Rorug05G0260100 Rorug05G0260200 Rorug05G0260300 Rorug05G0260400
rosa_samantha Rh5AG332100 Rh5BG342400 Rh5CG369600 Rh5DG355200
rosa_wichuraiana Rw5G031390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 10
AccIII TCCGGA 1 cut(s) 10
AcsI RAATTY 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 17, 53
AgsI TTSAA 1 cut(s) 160
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 291
Aor13HI TCCGGA 1 cut(s) 10
AoxI GGCC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 155
AseI ATTAAT 1 cut(s) 191
AspLEI GCGC 2 cut(s) 74, 165
BauI CACGAG 2 cut(s) 117, 247
BccI CCATC 2 cut(s) 54, 216
BciT130I CCWGG 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 291
BfoI RGCGCY 1 cut(s) 75
Bme1390I CCNGG 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 262
BpuEI CTTGAG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 7
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 53
Bse8I GATNNNNATC 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 10
BseBI CCWGG 1 cut(s) 262
BseGI GGATG 1 cut(s) 65
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 17, 53
BseMII CTCAG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 29
BshFI GGCC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 17, 53
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 207
Bsp13I TCCGGA 1 cut(s) 10
Bsp143I GATC 1 cut(s) 214
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 61
BspEI TCCGGA 1 cut(s) 10
BspHI TCATGA 1 cut(s) 211
BssMI GATC 1 cut(s) 214
BssNI GRCGYC 1 cut(s) 7
BssSI CACGAG 2 cut(s) 117, 247
Bst2BI CACGAG 2 cut(s) 117, 247
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 1 cut(s) 79
BstACI GRCGYC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 65
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 165
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 1 cut(s) 214
BstNI CCWGG 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 260
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 1 cut(s) 65
CciI TCATGA 1 cut(s) 211
CfoI GCGC 2 cut(s) 74, 165
CviAII CATG 3 cut(s) 185, 212, 297
CviJI RGCY 4 cut(s) 85, 93, 197, 207
CviKI_1 RGCY 4 cut(s) 85, 93, 197, 207
DdeI CTNAG 1 cut(s) 48
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EcoRI GAATTC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 260
FaeI CATG 3 cut(s) 188, 215, 300
FaiI YATR 5 cut(s) 96, 101, 186, 213, 298
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 184, 211, 296
FokI GGATG 1 cut(s) 72
GlaI GCGC 2 cut(s) 73, 164
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 11
HhaI GCGC 2 cut(s) 74, 165
Hin1I GRCGYC 1 cut(s) 7
Hin1II CATG 3 cut(s) 188, 215, 300
Hin6I GCGC 2 cut(s) 72, 163
HinP1I GCGC 2 cut(s) 72, 163
HinfI GANTC 2 cut(s) 239, 265
HpaII CCGG 1 cut(s) 11
Hpy188I TCNGA 4 cut(s) 36, 129, 235, 320
Hpy188III TCNNGA 3 cut(s) 11, 212, 247
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 2 cut(s) 26, 300
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 1 cut(s) 7
Hsp92I GRCGYC 1 cut(s) 7
Hsp92II CATG 3 cut(s) 188, 215, 300
HspAI GCGC 2 cut(s) 72, 163
Kpn2I TCCGGA 1 cut(s) 10
Kzo9I GATC 1 cut(s) 214
LpnPI CCDG 3 cut(s) 24, 247, 274
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 244
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MluCI AATT 4 cut(s) 155, 188, 274, 314
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 6 cut(s) 7, 10, 50, 57, 243, 296
MroI TCCGGA 1 cut(s) 10
MseI TTAA 3 cut(s) 108, 191, 273
MslI CAYNNNNRTG 1 cut(s) 234
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 262
MvaI CCWGG 1 cut(s) 262
NdeII GATC 1 cut(s) 214
NlaIII CATG 3 cut(s) 188, 215, 300
NmuCI GTSAC 1 cut(s) 244
PagI TCATGA 1 cut(s) 211
PfeI GAWTC 1 cut(s) 265
PflFI GACNNNGTC 1 cut(s) 242
PfoI TCCNGGA 1 cut(s) 260
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PshBI ATTAAT 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 234
SaqAI TTAA 3 cut(s) 108, 191, 273
Sau3AI GATC 1 cut(s) 214
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 262
SetI ASST 2 cut(s) 10, 95
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
Sse9I AATT 4 cut(s) 155, 188, 274, 314
StyD4I CCNGG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 1 cut(s) 10
TasI AATT 4 cut(s) 155, 188, 274, 314
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 3 cut(s) 108, 191, 273
Tru9I TTAA 3 cut(s) 108, 191, 273
TseFI GTSAC 1 cut(s) 244
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 3 cut(s) 195, 201, 285
TspGWI ACGGA 1 cut(s) 167
Tth111I GACNNNGTC 1 cut(s) 242
VspI ATTAAT 1 cut(s) 191
XapI RAATTY 1 cut(s) 155
ZraI GACGTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.