AT5G19850

Pheophytinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009477
Physical Location & Seq
Reverse (-)
6709734 .. 6711723
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19850.1

Sequence Viewer

Length: 1080 bp
ATGGCGAAGACTAGCATTTTTGCAGCTCTACCCTTTACCAACTTCGAATTCCCGTCGCTGTTCCGTGTCAAGAACTCTTCGATTATCAGTTTCAGCGAAACCCATTTTCTCCGACAATCAATTTCGACCGCAATCGTAAGATCTCCGACTAAGAGGGGAATTGTGTCCGTCTCATGTTCTTCCGTCACGGACGAAGCTTCTTCTGAAGAGCTACAAGTTCGAACCTTGACATGGAAGTGGAAAGGTTATTCGATAAGATATCAATGTGCTGGAACCAGTGGCCCTGCTTTGGTCTTGGTTCATGGTTTCGGTGCTAACAGTGACCATTGGAGGAAGAACACACCTATTCTTGGGAAAACACATAGGGTGTACTCAATCGATCTTATCGGATATGGATACTCCGATAAACCGAATCCTCGTGAGTTTGGTGGAGAACCATTCTACACTTTTGAGACATGGGGTGAGCAACTCAATGATTTCTGTCTGGATGTGGTCAAAGATGAAGCTTTCTTTATTTGCAACTCTATAGGAGGACTTGTTGGTCTTCAGGCAGCAGTAAGTAAGCCAGAGATATGCAGAGGGCTTATGCTTATTAACATATCCCTCCGGATGCTTCATATCAAGAAACAACCATTCATTGGAAGACCTTTCATCAAATCATTCCAGAATTTACTTAGGAACACTCCGGTTGGAAAATTATTTTTCAAGAGCATTGCTAAACCAGAGACAGTAAAGAGCATTCTTTGCCAGTGTTACCATGATAGCTCGCAAGTGACAGATGAACTAGTTGAGGCAATTCTACGTCCAGGGCTTGAGCCTGGTGCAGTTGATGTCTTCCTTGAATTTATCTGTTACTCCGGAGGACCACTTCCAGAGGATTTACTCCCACTAGTCAAGTGCCCGGTTCTAATTGCATGGGGAGAGAAAGATCCATGGGAGCCCATCGAGCTTGGACGAGCATATAGCAATTTTGACGCGGTTGAAGATTTCGTTGTTCTTCCAGATGCCGGCCACTGTCCTCAGGACGAGAAGCCGGAGATGGTGAACCCACTTATTGAATCATTCGTTGCGAGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

359

Amino Acids

40.1

Weight (kDa)

6.1

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 95 - 214 4e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 97 - 348 2.6e-19 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 638
AccII CGCG 1 cut(s) 977
AccIII TCCGGA 2 cut(s) 606, 857
AciI CCGC 2 cut(s) 129, 977
AclWI GGATC 1 cut(s) 923
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 3 cut(s) 47, 667, 842
AcuI CTGAAG 2 cut(s) 225, 530
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 5 cut(s) 231, 289, 350, 638, 1007
AgsI TTSAA 4 cut(s) 706, 842, 983, 1058
AhlI ACTAGT 2 cut(s) 784, 889
AjnI CCWGG 2 cut(s) 805, 817
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
AluBI AGCT 6 cut(s) 26, 197, 211, 506, 765, 949
AluI AGCT 6 cut(s) 26, 197, 211, 506, 765, 949
Alw26I GTCTC 4 cut(s) 175, 446, 719, 1066
AlwI GGATC 1 cut(s) 923
Aor13HI TCCGGA 2 cut(s) 606, 857
AoxI GGCC 2 cut(s) 280, 1009
ApeKI GCWGC 2 cut(s) 23, 551
ApoI RAATTY 3 cut(s) 47, 667, 842
AspS9I GGNCC 2 cut(s) 281, 863
AsuC2I CCSGG 1 cut(s) 902
AsuHPI GGTGA 2 cut(s) 473, 1054
AsuII TTCGAA 2 cut(s) 45, 220
AvaII GGWCC 1 cut(s) 863
AxyI CCTNAGG 1 cut(s) 1020
BaeGI GKGCMC 1 cut(s) 902
BanII GRGCYC 1 cut(s) 942
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 4 cut(s) 14, 536, 649, 826
BbvI GCAGC 2 cut(s) 35, 563
BccI CCATC 2 cut(s) 950, 1033
BciT130I CCWGG 2 cut(s) 807, 819
BciVI GTATCC 1 cut(s) 389
BcnI CCSGG 1 cut(s) 902
BcoDI GTCTC 4 cut(s) 175, 446, 719, 1066
BcuI ACTAGT 2 cut(s) 784, 889
BfaI CTAG 3 cut(s) 12, 785, 890
BfmI CTRYAG 1 cut(s) 525
BfuI GTATCC 1 cut(s) 389
BglII AGATCT 1 cut(s) 140
BisI GCNGC 2 cut(s) 24, 552
BlsI GCNGC 2 cut(s) 25, 553
Bme1390I CCNGG 3 cut(s) 807, 819, 902
Bme18I GGWCC 1 cut(s) 863
BmgT120I GGNCC 2 cut(s) 281, 863
BmiI GGNNCC 2 cut(s) 274, 939
BmrFI CCNGG 3 cut(s) 807, 819, 902
BmsI GCATC 2 cut(s) 600, 994
BpiI GAAGAC 4 cut(s) 14, 536, 649, 826
Bpu14I TTCGAA 2 cut(s) 45, 220
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 902
Bsa29I ATCGAT 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 806, 932
BsaWI WCCGGW 3 cut(s) 606, 685, 857
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 5 cut(s) 231, 289, 350, 638, 1007
Bse118I RCCGGY 1 cut(s) 1007
Bse1I ACTGG 2 cut(s) 276, 748
Bse21I CCTNAGG 1 cut(s) 1020
Bse3DI GCAATG 1 cut(s) 711
BseAI TCCGGA 2 cut(s) 606, 857
BseBI CCWGG 2 cut(s) 807, 819
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 2 cut(s) 806, 932
BseGI GGATG 2 cut(s) 493, 615
BseLI CCNNNNNNNGG 5 cut(s) 231, 289, 350, 638, 1007
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 1 cut(s) 1034
BseNI ACTGG 2 cut(s) 276, 748
BseSI GKGCMC 1 cut(s) 902
BseXI GCAGC 2 cut(s) 35, 563
BsgI GTGCAG 1 cut(s) 843
Bsh1236I CGCG 1 cut(s) 977
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 282, 1011
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 129
BsiSI CCGG 6 cut(s) 607, 686, 858, 902, 1008, 1034
BslI CCNNNNNNNGG 5 cut(s) 231, 289, 350, 638, 1007
BsmAI GTCTC 4 cut(s) 175, 446, 719, 1066
BsmBI CGTCTC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 738
BsnI GGCC 2 cut(s) 282, 1011
Bsp119I TTCGAA 2 cut(s) 45, 220
Bsp1286I GDGCHC 2 cut(s) 902, 942
Bsp13I TCCGGA 2 cut(s) 606, 857
Bsp143I GATC 3 cut(s) 140, 379, 928
Bsp19I CCATGG 1 cut(s) 932
BspACI CCGC 2 cut(s) 129, 977
BspANI GGCC 2 cut(s) 282, 1011
BspCNI CTCAG 1 cut(s) 1033
BspDI ATCGAT 1 cut(s) 378
BspEI TCCGGA 2 cut(s) 606, 857
BspFNI CGCG 1 cut(s) 977
BspLI GGNNCC 2 cut(s) 274, 939
BspPI GGATC 1 cut(s) 923
BspQI GCTCTTC 1 cut(s) 201
BspT104I TTCGAA 2 cut(s) 45, 220
BsrDI GCAATG 1 cut(s) 711
BsrFI RCCGGY 1 cut(s) 1007
BsrI ACTGG 2 cut(s) 276, 748
BssAI RCCGGY 1 cut(s) 1007
BssECI CCNNGG 2 cut(s) 806, 932
BssMI GATC 3 cut(s) 140, 379, 928
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 932
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 2 cut(s) 807, 819
Bst4CI ACNGT 3 cut(s) 320, 730, 1016
Bst6I CTCTTC 2 cut(s) 82, 201
BstAPI GCANNNNNTGC 1 cut(s) 744
BstBI TTCGAA 2 cut(s) 45, 220
BstC8I GCNNGC 2 cut(s) 767, 1009
BstDEI CTNAG 3 cut(s) 150, 674, 1020
BstDSI CCRYGG 1 cut(s) 932
BstF5I GGATG 2 cut(s) 493, 615
BstFNI CGCG 1 cut(s) 977
BstKTI GATC 3 cut(s) 143, 382, 931
BstMAI GTCTC 4 cut(s) 175, 446, 719, 1066
BstMBI GATC 3 cut(s) 140, 379, 928
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 2 cut(s) 744, 946
BstNI CCWGG 2 cut(s) 807, 819
BstSCI CCNGG 3 cut(s) 805, 817, 900
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 902
BstUI CGCG 1 cut(s) 977
BstV1I GCAGC 2 cut(s) 35, 563
BstV2I GAAGAC 4 cut(s) 14, 536, 649, 826
BstX2I RGATCY 2 cut(s) 140, 928
BstYI RGATCY 2 cut(s) 140, 928
Bsu15I ATCGAT 1 cut(s) 378
Bsu36I CCTNAGG 1 cut(s) 1020
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 2 cut(s) 282, 1011
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 932
BtsCI GGATG 2 cut(s) 493, 615
BtsIMutI CAGTG 4 cut(s) 283, 325, 755, 1012
Cac8I GCNNGC 2 cut(s) 767, 1009
Cfr10I RCCGGY 1 cut(s) 1007
Cfr13I GGNCC 2 cut(s) 281, 863
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 1 cut(s) 983
Csp6I GTAC 1 cut(s) 370
CviAII CATG 7 cut(s) 174, 231, 302, 456, 758, 915, 933
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 3 cut(s) 150, 674, 1020
DpnI GATC 3 cut(s) 142, 381, 930
DpnII GATC 3 cut(s) 140, 379, 928
DrdI GACNNNNNNGTC 1 cut(s) 540
DseDI GACNNNNNNGTC 1 cut(s) 540
EaeI YGGCCR 1 cut(s) 1009
Eam1104I CTCTTC 2 cut(s) 82, 201
EarI CTCTTC 2 cut(s) 82, 201
Eco130I CCWWGG 1 cut(s) 932
Eco24I GRGCYC 1 cut(s) 942
Eco32I GATATC 1 cut(s) 260
Eco47I GGWCC 1 cut(s) 863
Eco57I CTGAAG 2 cut(s) 225, 530
Eco81I CCTNAGG 1 cut(s) 1020
EcoRI GAATTC 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 805, 817
EcoRV GATATC 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 932
EcoT38I GRGCYC 1 cut(s) 942
ErhI CCWWGG 1 cut(s) 932
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 7 cut(s) 177, 234, 305, 459, 761, 918, 936
FatI CATG 7 cut(s) 173, 230, 301, 455, 757, 914, 932
Fnu4HI GCNGC 2 cut(s) 24, 552
FokI GGATG 2 cut(s) 500, 622
FriOI GRGCYC 1 cut(s) 942
Fsp4HI GCNGC 2 cut(s) 24, 552
FspBI CTAG 3 cut(s) 12, 785, 890
GluI GCNGC 2 cut(s) 24, 552
HaeIII GGCC 2 cut(s) 282, 1011
HapII CCGG 6 cut(s) 607, 686, 858, 902, 1008, 1034
HgaI GACGC 1 cut(s) 983
Hin1II CATG 7 cut(s) 177, 234, 305, 459, 761, 918, 936
HindIII AAGCTT 2 cut(s) 195, 504
HinfI GANTC 2 cut(s) 412, 1058
HpaII CCGG 6 cut(s) 607, 686, 858, 902, 1008, 1034
HphI GGTGA 2 cut(s) 473, 1054
Hpy166II GTNNAC 2 cut(s) 370, 1045
Hpy188I TCNGA 5 cut(s) 113, 147, 205, 389, 403
Hpy8I GTNNAC 2 cut(s) 370, 1045
Hpy99I CGWCG 1 cut(s) 58
HpyCH4III ACNGT 3 cut(s) 320, 730, 1016
HpyCH4IV ACGT 1 cut(s) 802
HpyCH4V TGCA 5 cut(s) 23, 519, 576, 824, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 744, 946
HpyF3I CTNAG 3 cut(s) 150, 674, 1020
HpySE526I ACGT 1 cut(s) 802
Hsp92II CATG 7 cut(s) 177, 234, 305, 459, 761, 918, 936
Kpn2I TCCGGA 2 cut(s) 606, 857
KroI GCCGGC 1 cut(s) 1007
KroNI GCCGGC 1 cut(s) 1009
Kzo9I GATC 3 cut(s) 140, 379, 928
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 1 cut(s) 937
Lsp1109I GCAGC 2 cut(s) 35, 563
LweI GCATC 2 cut(s) 600, 994
MaeI CTAG 3 cut(s) 12, 785, 890
MaeII ACGT 1 cut(s) 802
MaeIII GTNAC 5 cut(s) 184, 320, 752, 772, 851
MalI GATC 3 cut(s) 142, 381, 930
MboI GATC 3 cut(s) 140, 379, 928
MflI RGATCY 2 cut(s) 140, 928
MhlI GDGCHC 2 cut(s) 902, 942
MluCI AATT 9 cut(s) 47, 120, 159, 667, 695, 795, 842, 909, 967
MmeI TCCRAC 3 cut(s) 136, 170, 670
MroI TCCGGA 2 cut(s) 606, 857
MroNI GCCGGC 1 cut(s) 1007
MseI TTAA 1 cut(s) 594
MslI CAYNNNNRTG 1 cut(s) 235
MspI CCGG 6 cut(s) 607, 686, 858, 902, 1008, 1034
MspR9I CCNGG 3 cut(s) 807, 819, 902
Mva1269I GAATGC 1 cut(s) 738
MvaI CCWGG 2 cut(s) 807, 819
MvnI CGCG 1 cut(s) 977
MwoI GCNNNNNNNGC 2 cut(s) 744, 946
NaeI GCCGGC 1 cut(s) 1009
NciI CCSGG 1 cut(s) 902
NcoI CCATGG 1 cut(s) 932
NdeII GATC 3 cut(s) 140, 379, 928
NgoMIV GCCGGC 1 cut(s) 1007
NlaIII CATG 7 cut(s) 177, 234, 305, 459, 761, 918, 936
NlaIV GGNNCC 2 cut(s) 274, 939
NmuCI GTSAC 3 cut(s) 184, 320, 772
NspV TTCGAA 2 cut(s) 45, 220
PciSI GCTCTTC 1 cut(s) 201
PctI GAATGC 1 cut(s) 738
PdiI GCCGGC 1 cut(s) 1009
PfeI GAWTC 2 cut(s) 412, 1058
PflMI CCANNNNNTGG 1 cut(s) 638
PkrI GCNGC 2 cut(s) 25, 553
Psp6I CCWGG 2 cut(s) 805, 817
PspGI CCWGG 2 cut(s) 805, 817
PspN4I GGNNCC 2 cut(s) 274, 939
PspPI GGNCC 2 cut(s) 281, 863
PsuI RGATCY 2 cut(s) 140, 928
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 2 cut(s) 24, 552
Sau3AI GATC 3 cut(s) 140, 379, 928
Sau96I GGNCC 2 cut(s) 281, 863
ScrFI CCNGG 3 cut(s) 807, 819, 902
SduI GDGCHC 2 cut(s) 902, 942
SfaNI GCATC 2 cut(s) 600, 994
SfcI CTRYAG 1 cut(s) 525
SfuI TTCGAA 2 cut(s) 45, 220
SinI GGWCC 1 cut(s) 863
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
SpeI ACTAGT 2 cut(s) 784, 889
Sse9I AATT 9 cut(s) 47, 120, 159, 667, 695, 795, 842, 909, 967
SsiI CCGC 2 cut(s) 129, 977
SspMI CTAG 3 cut(s) 12, 785, 890
StyD4I CCNGG 3 cut(s) 805, 817, 900
StyI CCWWGG 1 cut(s) 932
TaaI ACNGT 3 cut(s) 320, 730, 1016
TaiI ACGT 1 cut(s) 805
TaqI TCGA 7 cut(s) 45, 80, 125, 220, 251, 378, 945
TasI AATT 9 cut(s) 47, 120, 159, 667, 695, 795, 842, 909, 967
TatI WGTACW 1 cut(s) 369
TfiI GAWTC 2 cut(s) 412, 1058
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 4 cut(s) 283, 325, 755, 1019
TseFI GTSAC 3 cut(s) 184, 320, 772
TseI GCWGC 2 cut(s) 23, 551
Tsp45I GTSAC 3 cut(s) 184, 320, 772
TspDTI ATGAA 6 cut(s) 290, 516, 605, 625, 640, 795
TspGWI ACGGA 4 cut(s) 53, 157, 172, 203
TspRI CASTG 4 cut(s) 283, 325, 755, 1019
Van91I CCANNNNNTGG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 863
XapI RAATTY 3 cut(s) 47, 667, 842
XspI CTAG 3 cut(s) 12, 785, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.