Rorug05G0483500

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009477
Physical Location & Seq
Reverse (-)
66523179 .. 66524364
1186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0483500.1

Sequence Viewer

Length: 477 bp
ATGTCGCTTATCCCCAATTTCCGACGTAACAGCAACATCTCTGACCCCTTTTCCCTCGATCTCTGGGACCCCTTCAAGGATTTTCCATTCCCTTCTTCATCGCTCTCTCCTTTTCCCGAATTTCCTCGCGAAAATTCGGCTTTTGTCAACACTAGGATCGACTGGAAGGAGACCCCGGAAGCCCATGTGTTCAAGGCTGACATTCCGGGGCTGACGAAAGAAGAGGTCAAGGTTGAGGTAGAAGATGACAGGGTGCTTCAGATCAGCGGAGAGAGGAAAATAGAGAAGGAGGACAAGAACGACACCTGGCACCGGGTCGAGAGAAGCAGCGGCAAGTTCTCCAGAAGGTTCAGGCTTCCTGAGAATGCCAAGATAGATGAAATCAAGGCTGCTATGGAGAACGGGGTTCTCAGCGTGACTGTTCCGAAGGCGGAGGTGAAAAGGCCTGATGTTAAAACCATTCAAATCTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

158

Amino Acids

18.2

Weight (kDa)

5.81

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 54 - 157 7.1e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 309
AccII CGCG 1 cut(s) 129
AciI CCGC 3 cut(s) 267, 330, 431
AclWI GGATC 1 cut(s) 164
AcsI RAATTY 2 cut(s) 119, 133
AcuI CTGAAG 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 76, 312
AgsI TTSAA 3 cut(s) 76, 193, 464
AjnI CCWGG 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 164
AoxI GGCC 1 cut(s) 443
ApeKI GCWGC 2 cut(s) 327, 389
ApoI RAATTY 2 cut(s) 119, 133
AspS9I GGNCC 1 cut(s) 67
AsuC2I CCSGG 3 cut(s) 176, 207, 314
AsuHPI GGTGA 1 cut(s) 448
AvaII GGWCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 339, 376
BciT130I CCWGG 1 cut(s) 307
BcnI CCSGG 3 cut(s) 176, 207, 314
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 153
BisI GCNGC 3 cut(s) 328, 331, 390
BlsI GCNGC 3 cut(s) 329, 332, 391
Bme1390I CCNGG 4 cut(s) 176, 207, 307, 314
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 3 cut(s) 68, 69, 311
BmrFI CCNGG 4 cut(s) 176, 207, 307, 314
BpmI CTGGAG 1 cut(s) 325
BpuMI CCSGG 3 cut(s) 176, 207, 314
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 174, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 312
Bse1I ACTGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 2 cut(s) 174, 206
BseLI CCNNNNNNNGG 2 cut(s) 76, 312
BseMII CTCAG 2 cut(s) 351, 424
BseNI ACTGG 1 cut(s) 167
BseXI GCAGC 2 cut(s) 339, 376
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 445
BshNI GGYRCC 1 cut(s) 309
BsiSI CCGG 3 cut(s) 176, 206, 313
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 76, 312
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 80
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 445
Bso31I GGTCTC 1 cut(s) 164
Bsp143I GATC 3 cut(s) 58, 156, 261
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 3 cut(s) 267, 330, 431
BspANI GGCC 1 cut(s) 445
BspCNI CTCAG 2 cut(s) 352, 423
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 3 cut(s) 68, 69, 311
BspPI GGATC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 174, 206
BssMI GATC 3 cut(s) 58, 156, 261
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 1 cut(s) 421
Bst6I CTCTTC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 360, 410
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 3 cut(s) 61, 159, 264
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 3 cut(s) 58, 156, 261
BstNI CCWGG 1 cut(s) 307
BstSCI CCNGG 4 cut(s) 174, 205, 305, 312
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 339, 376
BsuRI GGCC 1 cut(s) 445
BtgZI GCGATG 1 cut(s) 84
BtuMI TCGCGA 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 67
CviAII CATG 1 cut(s) 185
CviJI RGCY 7 cut(s) 140, 182, 197, 211, 355, 389, 445
CviKI_1 RGCY 7 cut(s) 140, 182, 197, 211, 355, 389, 445
DdeI CTNAG 2 cut(s) 360, 410
DpnI GATC 3 cut(s) 60, 158, 263
DpnII GATC 3 cut(s) 58, 156, 261
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
EciI GGCGGA 1 cut(s) 446
Eco147I AGGCCT 1 cut(s) 445
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 305
FaeI CATG 1 cut(s) 188
FaiI YATR 2 cut(s) 186, 395
FaqI GGGAC 1 cut(s) 80
FatI CATG 1 cut(s) 184
Fnu4HI GCNGC 3 cut(s) 328, 331, 390
Fsp4HI GCNGC 3 cut(s) 328, 331, 390
FspBI CTAG 1 cut(s) 153
GluI GCNGC 3 cut(s) 328, 331, 390
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 1 cut(s) 445
HapII CCGG 3 cut(s) 176, 206, 313
Hin1II CATG 1 cut(s) 188
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HpaII CCGG 3 cut(s) 176, 206, 313
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 4 cut(s) 23, 43, 261, 426
Hpy188III TCNNGA 5 cut(s) 116, 128, 319, 342, 359
Hpy8I GTNNAC 1 cut(s) 148
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 6 cut(s) 82, 102, 160, 280, 339, 421
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 1 cut(s) 25
HpyF3I CTNAG 2 cut(s) 360, 410
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 1 cut(s) 188
KflI GGGWCCC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 58, 156, 261
Lsp1109I GCAGC 2 cut(s) 339, 376
MaeI CTAG 1 cut(s) 153
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 2 cut(s) 26, 415
MalI GATC 3 cut(s) 60, 158, 263
MboI GATC 3 cut(s) 58, 156, 261
MboII GAAGA 3 cut(s) 87, 233, 254
MluCI AATT 3 cut(s) 16, 119, 133
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 7 cut(s) 65, 135, 217, 229, 267, 283, 427
MseI TTAA 2 cut(s) 453, 475
MspA1I CMGCKG 2 cut(s) 267, 330
MspI CCGG 3 cut(s) 176, 206, 313
MspR9I CCNGG 4 cut(s) 176, 207, 307, 314
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 1 cut(s) 307
MvnI CGCG 1 cut(s) 129
NciI CCSGG 3 cut(s) 176, 207, 314
NdeII GATC 3 cut(s) 58, 156, 261
NlaIII CATG 1 cut(s) 188
NlaIV GGNNCC 3 cut(s) 68, 69, 311
NmuCI GTSAC 1 cut(s) 415
NruI TCGCGA 1 cut(s) 129
PceI AGGCCT 1 cut(s) 445
PctI GAATGC 1 cut(s) 370
PkrI GCNGC 3 cut(s) 329, 332, 391
PpuMI RGGWCCY 1 cut(s) 67
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 305
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 3 cut(s) 68, 69, 311
PspPI GGNCC 1 cut(s) 67
PspPPI RGGWCCY 1 cut(s) 67
RruI TCGCGA 1 cut(s) 129
SaqAI TTAA 2 cut(s) 453, 475
SatI GCNGC 3 cut(s) 328, 331, 390
Sau3AI GATC 3 cut(s) 58, 156, 261
Sau96I GGNCC 1 cut(s) 67
ScrFI CCNGG 4 cut(s) 176, 207, 307, 314
SetI ASST 7 cut(s) 28, 228, 234, 240, 308, 350, 438
SinI GGWCC 1 cut(s) 67
Sse9I AATT 3 cut(s) 16, 119, 133
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 3 cut(s) 267, 330, 431
SspMI CTAG 1 cut(s) 153
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 4 cut(s) 174, 205, 305, 312
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 1 cut(s) 28
TaqI TCGA 3 cut(s) 57, 159, 318
TasI AATT 3 cut(s) 16, 119, 133
TauI GCSGC 1 cut(s) 333
Tru1I TTAA 2 cut(s) 453, 475
Tru9I TTAA 2 cut(s) 453, 475
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 2 cut(s) 327, 389
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 2 cut(s) 87, 393
VpaK11BI GGWCC 1 cut(s) 67
XapI RAATTY 2 cut(s) 119, 133
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.