Prupe.6G005000_v2.0.a1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0009477
Physical Location & Seq
Forward (+)
478433 .. 482702
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G005000.1

Sequence Viewer

Length: 1164 bp
ATGGCAAGCACTACCTCTCCCGCTCTGTCTGTGTCAAAATTGGATTTTCCATTTTTGTCCTCTCAGAGAAACATAACTTTCAGCAAGGAAAACCTTCATCAAGTTAAGATGCCTCGTAGGATGTCTAAGTTTTCTGGGTTTGAATCAAATGGGTTTATCTTATTTTCTTCTTTGAGTGGTCAAATTGGTTTGGGCAGCTTTCCTAGTCCAGAAGCCACTGCGAACAACAATGCGCCTGAGGTCCAAGTTCAAAGCAGTATATGGAACTGGAGGGGTTATTCTATCCGCTATCAGCATGCTGGCAACAGTGGCCCGGCATTGGTATTAATTCATGGTTTTGGAGCAAACAGTGACCATTGGAGGAAAAATATTCCAGTTCTTGCAAAATCACATAGGGTATACTCAATTGATCTTATTGGTTATGGGTACTCAGACAAACCAAATCCTCGTCAATTTGGGGAAAGTTCCTTTTATACATTTGAGACATGGGGCACCCAGCTAAATGACTTTTGTATTGATGTGGTCAAGGATGAAGCATTCTTTATATGCAACTCTATCGGAGTTTTTTTGGGTCTTCACACTGGACTTGTTGGTCTTCAGGCAGCAGTTATGGAGCCACAGTTATGTAAGGGCGTAATGCTCTTAAATATTTCTCTTCGTATGCTTCATATTAAAAAGCAGCCTTGGTACGGAAGACCATTGATTAGATCATTTCAGAACTTACTGAGAAATACTGATGTGGGCAAATATTTTTTCAAAATTGTTGCCACACCAGAGTCTGTGAGGAATATCCTTTGCCAGTGCTACCATGACACCTCCCAGGTAACAGAGGAACTAGTTCAAAAAATCCTTCTTCCAGGACTTGAGCCCGGTGCTGTAGATGTATTTCTGGAGTTCATTTGTTACTCAGGTAGCCCCCTTCCTGAGGAACTATTGCCTCAAGTGAAGTGCCCTGTTTTGATAGGATGGGGTGAGAAGGATCCATGGGAGCCCATTGAACTTGGACGTGCCTATGGGAAATTCGTTTCTGTAGAAGACTTCGTTGTTCTTCCTAATGTCGGCCACTGCCCTCAGGATGAAGCACCCGACCTAGTGAATCCACTGGTCGAGTCATTTGTGGCACGCCATGCTGCACTCTCAGCCAGCATTTCCACGGCTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

388

Amino Acids

43.01

Weight (kDa)

6.37

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 591
AccB1I GGYRCC 1 cut(s) 491
AccBSI CCGCTC 1 cut(s) 23
AccI GTMKAC 1 cut(s) 399
AciI CCGC 2 cut(s) 21, 286
AclWI GGATC 2 cut(s) 974, 987
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 1 cut(s) 1019
AcuI CTGAAG 1 cut(s) 581
AfaI GTAC 2 cut(s) 428, 689
AfiI CCNNNNNNNGG 4 cut(s) 319, 689, 925, 1058
AgsI TTSAA 5 cut(s) 143, 251, 757, 842, 998
AhlI ACTAGT 1 cut(s) 835
AjiI CACGTC 1 cut(s) 1007
AjnI CCWGG 2 cut(s) 819, 856
AluBI AGCT 2 cut(s) 198, 499
AluI AGCT 2 cut(s) 198, 499
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 2 cut(s) 974, 987
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 2 cut(s) 310, 1060
ApeKI GCWGC 4 cut(s) 195, 602, 679, 1130
ApoI RAATTY 1 cut(s) 1019
AseI ATTAAT 1 cut(s) 326
Asp700I GAANNNNTTC 2 cut(s) 93, 837
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 2 cut(s) 241, 311
AsuC2I CCSGG 2 cut(s) 314, 870
AsuHPI GGTGA 1 cut(s) 983
AvaII GGWCC 1 cut(s) 241
AxyI CCTNAGG 3 cut(s) 237, 924, 1071
BaeGI GKGCMC 2 cut(s) 494, 953
BamHI GGATCC 1 cut(s) 979
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 2 cut(s) 870, 993
BbsI GAAGAC 4 cut(s) 566, 587, 700, 1041
BbvI GCAGC 4 cut(s) 207, 614, 691, 1117
BccI CCATC 1 cut(s) 960
BcgI CGANNNNNNTGC 2 cut(s) 538, 572
BciT130I CCWGG 2 cut(s) 821, 858
BcnI CCSGG 2 cut(s) 314, 870
BcoDI GTCTC 1 cut(s) 476
BcuI ACTAGT 1 cut(s) 835
BfaI CTAG 3 cut(s) 204, 836, 1091
BfmI CTRYAG 2 cut(s) 876, 1029
BisI GCNGC 4 cut(s) 196, 603, 680, 1131
BlsI GCNGC 4 cut(s) 197, 604, 681, 1132
Bme1390I CCNGG 4 cut(s) 314, 821, 858, 870
Bme18I GGWCC 1 cut(s) 241
BmgBI CACGTC 1 cut(s) 1007
BmgT120I GGNCC 2 cut(s) 241, 311
BmiI GGNNCC 4 cut(s) 493, 615, 981, 990
BmrFI CCNGG 4 cut(s) 314, 821, 858, 870
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 4 cut(s) 566, 587, 700, 1041
BpmI CTGGAG 2 cut(s) 289, 911
BpuEI CTTGAG 2 cut(s) 884, 924
BpuMI CCSGG 2 cut(s) 314, 870
BsaJI CCNNGG 4 cut(s) 683, 819, 983, 1152
BsaXI ACNNNNNCTCC 3 cut(s) 31, 605, 635
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 689, 925, 1058
Bse1I ACTGG 5 cut(s) 272, 374, 586, 799, 1107
Bse21I CCTNAGG 3 cut(s) 237, 924, 1071
BseBI CCWGG 2 cut(s) 821, 858
BseDI CCNNGG 4 cut(s) 683, 819, 983, 1152
BseGI GGATG 4 cut(s) 126, 535, 971, 1081
BseLI CCNNNNNNNGG 4 cut(s) 319, 689, 925, 1058
BseMII CTCAG 8 cut(s) 77, 228, 444, 716, 915, 921, 1085, 1152
BseNI ACTGG 5 cut(s) 272, 374, 586, 799, 1107
BseSI GKGCMC 2 cut(s) 494, 953
BseXI GCAGC 4 cut(s) 207, 614, 691, 1117
BseYI CCCAGC 1 cut(s) 495
BsgI GTGCAG 1 cut(s) 1116
BshFI GGCC 2 cut(s) 312, 1062
BshNI GGYRCC 1 cut(s) 491
BsiSI CCGG 2 cut(s) 314, 870
BslI CCNNNNNNNGG 4 cut(s) 319, 689, 925, 1058
BsmAI GTCTC 1 cut(s) 476
BsmI GAATGC 1 cut(s) 536
BsnI GGCC 2 cut(s) 312, 1062
Bsp1286I GDGCHC 4 cut(s) 494, 870, 953, 993
Bsp143I GATC 3 cut(s) 409, 707, 979
Bsp19I CCATGG 1 cut(s) 983
BspACI CCGC 2 cut(s) 21, 286
BspANI GGCC 2 cut(s) 312, 1062
BspCNI CTCAG 8 cut(s) 76, 229, 443, 717, 916, 920, 1084, 1151
BspLI GGNNCC 4 cut(s) 493, 615, 981, 990
BspPI GGATC 2 cut(s) 974, 987
BspT107I GGYRCC 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 23
BsrI ACTGG 5 cut(s) 272, 374, 586, 799, 1107
BssECI CCNNGG 4 cut(s) 683, 819, 983, 1152
BssMI GATC 3 cut(s) 409, 707, 979
BssNAI GTATAC 1 cut(s) 400
BssT1I CCWWGG 2 cut(s) 683, 983
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 2 cut(s) 821, 858
Bst4CI ACNGT 3 cut(s) 308, 350, 621
Bst6I CTCTTC 1 cut(s) 660
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstC8I GCNNGC 5 cut(s) 7, 297, 301, 1123, 1144
BstDEI CTNAG 9 cut(s) 63, 126, 237, 430, 725, 907, 924, 1071, 1138
BstDSI CCRYGG 2 cut(s) 983, 1152
BstENI CCTNNNNNAGG 1 cut(s) 923
BstF5I GGATG 4 cut(s) 126, 535, 971, 1081
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 3 cut(s) 412, 710, 982
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 3 cut(s) 409, 707, 979
BstMWI GCNNNNNNNGC 3 cut(s) 309, 1127, 1139
BstNI CCWGG 2 cut(s) 821, 858
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 4 cut(s) 312, 819, 856, 868
BstSFI CTRYAG 2 cut(s) 876, 1029
BstSLI GKGCMC 2 cut(s) 494, 953
BstV1I GCAGC 4 cut(s) 207, 614, 691, 1117
BstV2I GAAGAC 4 cut(s) 566, 587, 700, 1041
BstX2I RGATCY 1 cut(s) 979
BstYI RGATCY 1 cut(s) 979
BstZ17I GTATAC 1 cut(s) 400
Bsu36I CCTNAGG 3 cut(s) 237, 924, 1071
BsuRI GGCC 2 cut(s) 312, 1062
BtgI CCRYGG 2 cut(s) 983, 1152
BtrI CACGTC 1 cut(s) 1007
BtsCI GGATG 4 cut(s) 126, 535, 971, 1081
BtsI GCAGTG 2 cut(s) 216, 1063
BtsIMutI CAGTG 7 cut(s) 216, 313, 355, 579, 806, 1063, 1100
Cac8I GCNNGC 5 cut(s) 7, 297, 301, 1123, 1144
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 241, 311
Csp6I GTAC 2 cut(s) 427, 688
CviAII CATG 6 cut(s) 296, 332, 486, 809, 984, 1127
CviQI GTAC 2 cut(s) 427, 688
DdeI CTNAG 9 cut(s) 63, 126, 237, 430, 725, 907, 924, 1071, 1138
DpnI GATC 3 cut(s) 411, 709, 981
DpnII GATC 3 cut(s) 409, 707, 979
DrdI GACNNNNNNGTC 1 cut(s) 591
DseDI GACNNNNNNGTC 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco130I CCWWGG 2 cut(s) 683, 983
Eco24I GRGCYC 2 cut(s) 870, 993
Eco47I GGWCC 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 581
Eco81I CCTNAGG 3 cut(s) 237, 924, 1071
EcoNI CCTNNNNNAGG 1 cut(s) 923
EcoRII CCWGG 2 cut(s) 819, 856
EcoT14I CCWWGG 2 cut(s) 683, 983
EcoT38I GRGCYC 2 cut(s) 870, 993
ErhI CCWWGG 2 cut(s) 683, 983
FaeI CATG 6 cut(s) 299, 335, 489, 812, 987, 1130
FatI CATG 6 cut(s) 295, 331, 485, 808, 983, 1126
FauI CCCGC 1 cut(s) 28
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 4 cut(s) 196, 603, 680, 1131
FokI GGATG 4 cut(s) 133, 542, 978, 1088
FriOI GRGCYC 2 cut(s) 870, 993
Fsp4HI GCNGC 4 cut(s) 196, 603, 680, 1131
FspBI CTAG 3 cut(s) 204, 836, 1091
GlaI GCGC 1 cut(s) 234
GluI GCNGC 4 cut(s) 196, 603, 680, 1131
GsaI CCCAGC 1 cut(s) 499
GsuI CTGGAG 2 cut(s) 289, 911
HaeIII GGCC 2 cut(s) 312, 1062
HapII CCGG 2 cut(s) 314, 870
HhaI GCGC 1 cut(s) 235
Hin1II CATG 6 cut(s) 299, 335, 489, 812, 987, 1130
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HinfI GANTC 4 cut(s) 143, 776, 1096, 1109
HpaII CCGG 2 cut(s) 314, 870
HphI GGTGA 1 cut(s) 983
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 4 cut(s) 66, 433, 560, 717
Hpy188III TCNNGA 4 cut(s) 209, 890, 923, 1073
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 4 cut(s) 104, 860, 929, 970
HpyCH4III ACNGT 3 cut(s) 308, 350, 621
HpyCH4IV ACGT 1 cut(s) 1006
HpyCH4V TGCA 3 cut(s) 383, 549, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 1127, 1139
HpyF3I CTNAG 9 cut(s) 63, 126, 237, 430, 725, 907, 924, 1071, 1138
HpySE526I ACGT 1 cut(s) 1006
Hsp92II CATG 6 cut(s) 299, 335, 489, 812, 987, 1130
HspAI GCGC 1 cut(s) 233
Kzo9I GATC 3 cut(s) 409, 707, 979
LmnI GCTCC 3 cut(s) 341, 613, 988
Lsp1109I GCAGC 4 cut(s) 207, 614, 691, 1117
LweI GCATC 1 cut(s) 99
MaeI CTAG 3 cut(s) 204, 836, 1091
MaeII ACGT 1 cut(s) 1006
MaeIII GTNAC 3 cut(s) 350, 823, 902
MalI GATC 3 cut(s) 411, 709, 981
MbiI CCGCTC 1 cut(s) 23
MboI GATC 3 cut(s) 409, 707, 979
MboII GAAGA 8 cut(s) 159, 566, 587, 647, 705, 845, 1040, 1046
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 4 cut(s) 494, 870, 953, 993
MluCI AATT 7 cut(s) 38, 183, 327, 405, 452, 759, 1019
MlyI GAGTC 2 cut(s) 785, 1118
MroXI GAANNNNTTC 2 cut(s) 93, 837
MseI TTAA 4 cut(s) 105, 326, 644, 672
MslI CAYNNNNRTG 1 cut(s) 622
MspI CCGG 2 cut(s) 314, 870
MspR9I CCNGG 4 cut(s) 314, 821, 858, 870
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 1 cut(s) 536
MvaI CCWGG 2 cut(s) 821, 858
MwoI GCNNNNNNNGC 3 cut(s) 309, 1127, 1139
NciI CCSGG 2 cut(s) 314, 870
NcoI CCATGG 1 cut(s) 983
NdeII GATC 3 cut(s) 409, 707, 979
NlaIII CATG 6 cut(s) 299, 335, 489, 812, 987, 1130
NlaIV GGNNCC 4 cut(s) 493, 615, 981, 990
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 299
PaeI GCATGC 1 cut(s) 299
PctI GAATGC 1 cut(s) 536
PdmI GAANNNNTTC 2 cut(s) 93, 837
PfeI GAWTC 2 cut(s) 143, 1096
PfoI TCCNGGA 1 cut(s) 856
PkrI GCNGC 4 cut(s) 197, 604, 681, 1132
PleI GAGTC 2 cut(s) 784, 1117
PpsI GAGTC 2 cut(s) 784, 1117
PshBI ATTAAT 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 819, 856
PspFI CCCAGC 1 cut(s) 495
PspGI CCWGG 2 cut(s) 819, 856
PspN4I GGNNCC 4 cut(s) 493, 615, 981, 990
PspPI GGNCC 2 cut(s) 241, 311
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 979
RsaI GTAC 2 cut(s) 428, 689
RsaNI GTAC 2 cut(s) 427, 688
RseI CAYNNNNRTG 1 cut(s) 622
SaqAI TTAA 4 cut(s) 105, 326, 644, 672
SatI GCNGC 4 cut(s) 196, 603, 680, 1131
Sau3AI GATC 3 cut(s) 409, 707, 979
Sau96I GGNCC 2 cut(s) 241, 311
SchI GAGTC 2 cut(s) 785, 1118
ScrFI CCNGG 4 cut(s) 314, 821, 858, 870
SduI GDGCHC 4 cut(s) 494, 870, 953, 993
SfaNI GCATC 1 cut(s) 99
SfcI CTRYAG 2 cut(s) 876, 1029
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 622
SmlI CTYRAG 2 cut(s) 863, 939
SmoI CTYRAG 2 cut(s) 863, 939
SpeI ACTAGT 1 cut(s) 835
SphI GCATGC 1 cut(s) 299
Sse9I AATT 7 cut(s) 38, 183, 327, 405, 452, 759, 1019
SsiI CCGC 2 cut(s) 21, 286
SspI AATATT 3 cut(s) 370, 649, 749
SspMI CTAG 3 cut(s) 204, 836, 1091
StyD4I CCNGG 4 cut(s) 312, 819, 856, 868
StyI CCWWGG 2 cut(s) 683, 983
TaaI ACNGT 3 cut(s) 308, 350, 621
TaiI ACGT 1 cut(s) 1009
TaqI TCGA 1 cut(s) 1107
TasI AATT 7 cut(s) 38, 183, 327, 405, 452, 759, 1019
TfiI GAWTC 2 cut(s) 143, 1096
Tru1I TTAA 4 cut(s) 105, 326, 644, 672
Tru9I TTAA 4 cut(s) 105, 326, 644, 672
TscAI CASTG 7 cut(s) 223, 313, 355, 586, 806, 1070, 1107
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 4 cut(s) 195, 602, 679, 1130
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 6 cut(s) 86, 320, 546, 656, 886, 1092
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 7 cut(s) 223, 313, 355, 586, 806, 1070, 1107
VpaK11BI GGWCC 1 cut(s) 241
VspI ATTAAT 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 923
XapI RAATTY 1 cut(s) 1019
XceI RCATGY 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 2 cut(s) 93, 837
XspI CTAG 3 cut(s) 204, 836, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.