AT5G20320

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004795
Physical Location & Seq
Reverse (-)
6859212 .. 6869307
10096 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20320.1

Sequence Viewer

Length: 5109 bp
ATGCGTGACGAAGTTGACTTGAGCTTGACCATTCCCTCGAAGCTTTTGGGGAAGCGAGACAGAGAACAAAAAAATTGTGAAGAAGAAAAAAACAAAAACAAAAAAGCTAAAAAGCAGCAAAAGGACCCAATTCTTCTTCACACTAGTGCTGCCACTCACAAGTTTCTTCCTCCTCCTTTGACCATGCCGTACAGTGAAATCGGCGACGATCTTCGCTCACTCGACTTTGACCACGCCGATGTTTCTTCCGACCTTCACCTCACTTCTTCTTCCTCTGTTTCTTCGTTTTCCTCTTCTTCGTCTTCTTTGTTCTCCGCGGCTGGTACGGATGATCCTTCACCGAAAATGGAGAAAGACCCTAGAAAAATCGCCAGGAGGTATCAGGTGGAGCTGTGTAAGAAAGCAACGGAGGAGAACGTTATTGTATATTTGGGTACAGGTTGTGGGAAGACTCACATTGCAGTGATGCTTATATATGAGCTTGGTCATTTGGTTCTTAGTCCCAAGAAAAGTGTTTGTATTTTTCTTGCTCCCACCGTGGCTTTGGTCGAACAGCAAGCCAAGGTCATAGCGGACTCTGTCAACTTCAAAGTTGCAATACATTGTGGAGGCAAGAGGATTGTGAAGAGCCACTCGGAGTGGGAGAGAGAGATTGCAGCGAATGAGGTTCTTGTTATGACTCCACAAATACTTCTGCATAACTTACAGCACTGTTTCATCAAGATGGAGTGTATCTCCCTTCTAATATTTGATGAGTGTCACCATGCTCAACAACAAAGCAACCATCCTTATGCAGAAATCATGAAGGTTTTCTATAAATCGGAAAGTTTACAACGGCCTCGAATATTTGGAATGACTGCATCTCCAGTTGTTGGCAAAGGGTCTTTTCAATCAGAGAATTTATCGAAAAGCATTAATAGCCTTGAAAATTTGCTCAATGCCAAGGTTTATTCAGTGGAAAGCAATGTCCAGCTGGATGGTTTTGTTTCATCTCCTTTAGTCAAAGTATATTATTATCGGTCAGCTTTAAGTGATGCATCTCAATCGACCATCAGATATGAAAACATGCTGGAGGACATCAAACAGCGGTGCTTGGCATCACTTAAGCTGCTGATTGATACTCATCAAACACAAACCCTCCTAAGTATGAAAAGGCTTCTCAAAAGATCTCATGATAATCTCATATATACTCTGCTGAATCTTGGCCTCTGGGGAGCAATACAGGCTGCTAAAATCCAATTGAATAGTGACCATAATGTACAAGACGAGCCTGTGGGAAAGAATCCTAAGTCAAAGATATGTGATACATATCTTTCTATGGCTGCTGAGGCCCTCTCTTCTGGTGTTGCTAAAGATGAGAATGCATCTGACCTCCTCAGCTTAGCGGCGTTGAAGGAACCATTATTCTCTAGAAAGCTAGTTCAATTGATTAAGATCCTTTCGGTATTCAGGCTAGAGCCACACATGAAATGTATAATATTTGTCAATCGGATTGTGACTGCAAGAACATTGTCATGCATACTAAATAACTTGGAACTGCTACGGTCTTGGAAGTCTGATTTCCTTGTTGGACTTAGTTCTGGACTGAAGAGCATGTCAAGAAGGAGTATGGAAACAATACTTAAACGGTTCCAATCTAAAGAGCTCAATTTACTGGTTGCCACTAAAGTTGGTGAAGAAGGCCTTGATATTCAGACATGCTGTCTTGTGATCCGTTATGATTTACCAGAGACTGTTACCAGCTTCATACAGTCCAGAGGTCGTGCTCGAATGCCTCAGTCTGAATATGCGTTTCTAGTGGACAGCGGAAACGAGAAAGAGATGGATCTTATTGAAAATTTTAAAGTAAATGAAGATCGAATGAATCTAGAAATTACTTACAGAAGCTCAGAGGAAACTTGTCCTAGACTTGATGAGGAGTTATACAAAGTTCATGAGACAGGAGCTTGTATCAGTGGTGGAAGCAGCATCTCCCTTCTCTATAAATATTGTTCTAGGCTTCCACATGATGAATTTTTTCAGCCCAAGCCAGAGTTTCAATTCAAGCCTGTTGACGAATTTGGTGGAACTATCTGTCGCATAACTTTACCTGCTAATGCTCCTATAAGTGAAATCGAAAGTTCACTACTACCTTCGACAGAAGCTGCTAAAAAGGATGCTTGTCTAAAGGCTGTGCATGAGTTGCACAACTTGGGTGTACTTAACGATTTTCTGTTGCCAGATTCCAAGGATGAAATTGAGGACGAATTGTCAGATGATGAATTTGATTTTGATAACATCAAAGGTGAAGGCTGTTCACGAGGTGACCTGTATGAGATGCGTGTACCAGTCTTGTTTAAACAAAAGTGGGATCCATCTACAAGTTGTGTCAATCTTCATTCTTACTATATAATGTTTGTGCCTCATCCCGCTGATAGGATCTACAAAAAGTTTGGTTTCTTCATGAAGTCACCTCTTCCCGTTGAGGCTGAGACTATGGATATCGATCTTCACCTTGCTCATCAAAGATCTGTAAGTGTAAAGATTTTTCCATCAGGGGTCACAGAATTCGACAACGATGAGATAAGACTAGCTGAGCTTTTCCAGGAGATTGCCCTGAAGGTTCTTTTTGAACGGGGGGAGCTGATCCCGGACTTTGTTCCCTTGGAACTGCAAGACTCTTCTAGAACAAGCAAATCCACCTTCTACCTTCTTCTTCCACTCTGTCTGCATGATGGAGAAAGTGTTATATCTGTAGATTGGGTGACTATCAGAAACTGCTTGTCATCACCAATCTTTAAGACTCCATCTGTTTTAGTGGAAGATATATTTCCTCCTTCGGGCTCTCATTTAAAGCTAGCAAATGGCTGCTGGAATATTGATGATGTGAAGAACAGCTTGGTTTTTACAACCTACAGTAAACAATTTTACTTTGTTGCTGATATCTGCCATGGAAGAAATGGTTTCAGTCCTGTTAAGGAATCTAGCACCAAAAGCCATGTGGAGAGCATATATAAGTTGTATGGCGTGGAACTCAAGCATCCTGCACAGCCACTCTTGCGTGTGAAACCACTTTGTCATGTTCGGAACTTGCTTCACAACCGAATGCAGACGAATTTGGAACCACAAGAACTTGACGAATACTTCATAGAGATTCCTCCCGAACTTTCTCACTTAAAGATAAAAGGATTATCTAAAGACATCGGAAGCTCGTTATCCTTGTTACCATCAATCATGCATCGTATGGAGAATTTACTCGTGGCTATTGAACTGAAACATGTGCTGTCTGCTTCGATCCCTGAGATAGCTGAAGTTTCTGGTCACAGGGTACTCGAGGCGCTCACAACAGAGAAATGTCATGAGCGCCTTTCTCTTGAAAGGCTTGAGGTGCTTGGTGATGCATTCCTCAAGTTTGCTGTTAGCCGACACCTTTTTCTACACCATGATAGTCTTGATGAAGGAGAGTTGACTCGGAGACGCTCTAACGTTGTTAACAATTCCAACTTGTGCAGGCTTGCAATAAAAAAAAATCTGCAGGTCTACATCCGTGATCAAGCATTGGATCCTACTCAGTTCTTTGCATTTGGCCATCCATGCAGAGTAACCTGTGACGAGGTAGCCAGTAAAGAGGTTCATTCCTTGAATAGGGATCTTGGGATCTTGGAGTCAAATACTGGTGAAATCAGATGTAGCAAAGGCCATCATTGGTTGTACAAGAAAACAATTGCTGATGTGGTTGAGGCTCTTGTGGGAGCTTTCTTAGTTGACAGTGGCTTCAAAGGTGCTGTGAAATTTCTGAAGTGGATTGGTGTAAATGTTGATTTTGAATCCTTGCAAGTACAAGATGCTTGTATTGCAAGCAGGCGCTACTTGCCCCTCACTACTCGCAATAATTTGGAGACCCTTGAAAACCAGCTTGACTATAAGTTCCTCCACAAAGGTCTACTTGTACAAGCCTTTATCCATCCATCTTACAACAGGCATGGAGGAGGCTGCTACCAGAGATTGGAGTTTCTTGGGGATGCTGTTCTGGACTACTTGATGACATCCTATTTTTTCACAGTCTTCCCGAAACTGAAACCTGGTCAACTGACCGATCTAAGATCTCTCTCAGTAAATAATGAGGCGCTAGCAAATGTTGCTGTCAGTTTTTCGCTAAAGAGATTTCTATTTTGCGAGTCCATTTATCTTCATGAAGTTATAGAGGATTATACCAATTTCCTGGCATCTTCCCCATTGGCAAGTGGACAATCTGAAGGTCCAAGATGCCCAAAGGTTCTTGGTGACTTGGTAGAATCCTGTTTGGGGGCTCTTTTCCTCGATTGTGGGTTCAACTTGAATCATGTCTGGACTATGATGCTATCATTTCTAGATCCGGTCAAAAACTTGTCTAACCTTCAGATTAGTCCTATAAAAGAACTGATTGAACTTTGCCAGTCTTACAAGTGGGATCGGGAAATATCAGCGACGAAAAAGGATGGTGCTTTTACTGTTGAACTAAAAGTGACCAAGAATGGTTGTTGCCTTACAGTTTCTGCAACTGGTCGGAACAAAAGAGAGGGCACAAAAAAGGCTGCACAGCTGATGATTACAAACCTGAAGGCTCATGAGAACATAACAACCTCCCATCCGTTGGAGGATGTTCTGAAGAATGGCATCCGAAATGAAGCTAAATTAATTGGCTACAATGAAGATCCTATAGATGTTGTGGATCTTGTTGGGCTGGACGTTGAAAACCTAAATATCCTAGAAACTTTTGGCGGGAATAGTGAAAGAAGCAGCTCATACGTCATCAGACGAGGTCTCCCCCAAGCACCATCTAAAACAGAAGACAGGCTTCCTCAAAAGGCCATCATAAAAGCAGGTGGACCAAGCAGCAAAACCGCAAAATCCCTCTTGCACGAAACATGTGTTGCTAACTGTTGGAAGCCACCACACTTCGAATGTTGTGAAGAGGAAGGACCAGGCCACCTGAAATCATTCGTCTACAAGGTAATCCTGGAAGTTGAAGATGCGCCCAATATGACATTGGAATGTTATGGTGAGGCTAGAGCAACGAAGAAAGGTGCAGCAGAGCACGCTGCCCAAGCTGCTATATGGTGCCTCAAGCATTCTGGATTCCTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1702

Amino Acids

191.28

Weight (kDa)

6.3

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 125 - 289 9.7e-16 Type III restriction enzyme, res subunit
DEAD PF00270 126 - 289 8.2e-19 DEAD/DEAH box helicase
Helicase_C PF00271 474 - 589 6.2e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 656 - 744 3.6e-24 Dicer dimerisation domain
Ribonucleas_3_3 PF14622 1118 - 1176 1.1e-08 Ribonuclease-III-like
Ribonuclease_3 PF00636 1119 - 1251 5.3e-20 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1307 - 1438 5.1e-16 Ribonuclease-III-like
Ribonuclease_3 PF00636 1327 - 1436 8.9e-23 Ribonuclease III domain
DND1_DSRM PF14709 1621 - 1696 1.3e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1622 - 1692 2.2e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4832
Acc36I ACCTGC 3 cut(s) 2098, 3505, 4832
AccB1I GGYRCC 1 cut(s) 5079
AccB7I CCANNNNNTGG 3 cut(s) 872, 3985, 4612
AccI GTMKAC 3 cut(s) 3519, 3922, 4965
AccII CGCG 1 cut(s) 317
AciI CCGC 9 cut(s) 315, 317, 572, 1087, 1385, 1806, 2409, 4740, 4863
AclI AACGTT 2 cut(s) 417, 3465
AcoI YGGCCR 1 cut(s) 3565
AcuI CTGAAG 8 cut(s) 1607, 2618, 3309, 3797, 4254, 4361, 4598, 4646
AdeI CACNNNGTG 1 cut(s) 2303
AfiI CCNNNNNNNGG 7 cut(s) 872, 2415, 2819, 3624, 3985, 4284, 4612
AflII CTTAAG 1 cut(s) 1103
AflIII ACRYGT 2 cut(s) 3256, 4886
AhdI GACNNNNNGTC 2 cut(s) 1701, 2248
AhlI ACTAGT 1 cut(s) 143
AjnI CCWGG 6 cut(s) 371, 2583, 4060, 4200, 4942, 4977
AjuI GAANNNNNNNTTGG 2 cut(s) 2861, 2893
AleI CACNNNNGTG 1 cut(s) 144
Alw21I GWGCWC 3 cut(s) 1647, 1768, 5058
Alw26I GTCTC 7 cut(s) 51, 1724, 1931, 2465, 3448, 3872, 4787
AlwNI CAGNNNCTG 4 cut(s) 1733, 2144, 2757, 4514
Ama87I CYCGRG 1 cut(s) 3311
AoxI GGCC 8 cut(s) 836, 1204, 1329, 1681, 3565, 3676, 4827, 4945
AseI ATTAAT 2 cut(s) 915, 4655
Asp700I GAANNNNTTC 3 cut(s) 809, 2016, 4958
AspLEI GCGC 5 cut(s) 3319, 3345, 3846, 4108, 4996
AspS9I GGNCC 5 cut(s) 124, 1330, 4238, 4847, 4940
AsuC2I CCSGG 1 cut(s) 2630
AsuII TTCGAA 1 cut(s) 4920
AsuNHI GCTAGC 2 cut(s) 2836, 4108
AvaI CYCGRG 1 cut(s) 3311
AvaII GGWCC 4 cut(s) 124, 4238, 4847, 4940
BaeGI GKGCMC 1 cut(s) 4544
BalI TGGCCA 1 cut(s) 3567
BamHI GGATCC 2 cut(s) 2350, 3541
BanI GGYRCC 1 cut(s) 5079
BanII GRGCYC 3 cut(s) 1647, 2825, 4291
BauI CACGAG 2 cut(s) 2298, 3236
BbsI GAAGAC 4 cut(s) 294, 455, 4036, 4815
Bbv12I GWGCWC 3 cut(s) 1647, 1768, 5058
BbvCI CCTCAGC 2 cut(s) 1326, 1376
BceAI ACGGC 2 cut(s) 172, 851
BcgI CGANNNNNNTGC 2 cut(s) 1026, 1060
BciT130I CCWGG 6 cut(s) 373, 2585, 4062, 4202, 4944, 4979
BclI TGATCA 1 cut(s) 3529
BcnI CCSGG 1 cut(s) 2630
BcoDI GTCTC 7 cut(s) 51, 1724, 1931, 2465, 3448, 3872, 4787
BcuI ACTAGT 1 cut(s) 143
BfmI CTRYAG 4 cut(s) 2733, 2893, 3512, 4677
BfoI RGCGCY 4 cut(s) 3320, 3346, 3847, 4109
BfrI CTTAAG 1 cut(s) 1103
BfuAI ACCTGC 3 cut(s) 2098, 3505, 4832
BglII AGATCT 3 cut(s) 1166, 2507, 4082
BlpI GCTNAGC 2 cut(s) 1381, 2574
Bme1390I CCNGG 7 cut(s) 373, 2585, 2630, 4062, 4202, 4944, 4979
Bme18I GGWCC 4 cut(s) 124, 4238, 4847, 4940
BmeRI GACNNNNNGTC 2 cut(s) 1701, 2248
BmeT110I CYCGRG 1 cut(s) 3311
BmgT120I GGNCC 5 cut(s) 124, 1330, 4238, 4847, 4940
BmiI GGNNCC 7 cut(s) 126, 1398, 1631, 2352, 3102, 3543, 5081
BmrFI CCNGG 7 cut(s) 373, 2585, 2630, 4062, 4202, 4944, 4979
BmtI GCTAGC 2 cut(s) 2840, 4112
BpiI GAAGAC 4 cut(s) 294, 455, 4036, 4815
BplI GAGNNNNNCTC 4 cut(s) 719, 751, 1319, 1351
BpmI CTGGAG 2 cut(s) 849, 1091
Bpu10I CCTNAGC 2 cut(s) 1326, 1376
Bpu1102I GCTNAGC 2 cut(s) 1381, 2574
Bpu14I TTCGAA 1 cut(s) 4920
BpuEI CTTGAG 5 cut(s) 40, 2999, 3371, 3383, 5069
BpuMI CCSGG 1 cut(s) 2630
Bsa29I ATCGAT 1 cut(s) 2484
BsaBI GATNNNNATC 4 cut(s) 1122, 1308, 1433, 2484
BsaI GGTCTC 2 cut(s) 3872, 4787
BsaJI CCNNGG 7 cut(s) 315, 537, 561, 942, 2226, 2643, 2929
BsaWI WCCGGW 1 cut(s) 4354
BsaXI ACNNNNNCTCC 8 cut(s) 847, 877, 1122, 1152, 2709, 2739, 4767, 4797
Bsc4I CCNNNNNNNGG 7 cut(s) 872, 2415, 2819, 3624, 3985, 4284, 4612
Bse1I ACTGG 7 cut(s) 866, 1659, 2327, 3600, 3658, 4414, 4525
Bse3DI GCAATG 2 cut(s) 456, 970
Bse8I GATNNNNATC 4 cut(s) 1122, 1308, 1433, 2484
BseBI CCWGG 6 cut(s) 373, 2585, 4062, 4202, 4944, 4979
BseCI ATCGAT 1 cut(s) 2484
BseDI CCNNGG 7 cut(s) 315, 537, 561, 942, 2226, 2643, 2929
BseJI GATNNNNATC 4 cut(s) 1122, 1308, 1433, 2484
BseLI CCNNNNNNNGG 7 cut(s) 872, 2415, 2819, 3624, 3985, 4284, 4612
BseMI GCAATG 2 cut(s) 456, 970
BseMII CTCAG 9 cut(s) 1317, 1390, 1790, 1902, 2460, 2565, 3270, 3563, 4104
BseNI ACTGG 7 cut(s) 866, 1659, 2327, 3600, 3658, 4414, 4525
BseRI GAGGAG 5 cut(s) 162, 425, 1364, 1931, 3981
BseSI GKGCMC 1 cut(s) 4544
BsgI GTGCAG 4 cut(s) 3009, 3508, 4539, 5067
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 8 cut(s) 838, 1206, 1331, 1683, 3567, 3678, 4829, 4947
BshNI GGYRCC 1 cut(s) 5079
BshVI ATCGAT 1 cut(s) 2484
BsiHKAI GWGCWC 3 cut(s) 1647, 1768, 5058
BsiHKCI CYCGRG 1 cut(s) 3311
BsiSI CCGG 2 cut(s) 2630, 4355
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 7 cut(s) 872, 2415, 2819, 3624, 3985, 4284, 4612
BsmAI GTCTC 7 cut(s) 51, 1724, 1931, 2465, 3448, 3872, 4787
BsmBI CGTCTC 1 cut(s) 3448
BsmFI GGGAC 1 cut(s) 486
BsmI GAATGC 5 cut(s) 1366, 1776, 3090, 3380, 5089
BsnI GGCC 8 cut(s) 838, 1206, 1331, 1683, 3567, 3678, 4829, 4947
Bso31I GGTCTC 2 cut(s) 3872, 4787
BsoBI CYCGRG 1 cut(s) 3311
Bsp119I TTCGAA 1 cut(s) 4920
Bsp1286I GDGCHC 6 cut(s) 1647, 1768, 2825, 4291, 4544, 5058
Bsp1407I TGTACA 3 cut(s) 1258, 3690, 3928
Bsp1720I GCTNAGC 2 cut(s) 1381, 2574
Bsp19I CCATGG 1 cut(s) 2929
BspACI CCGC 9 cut(s) 315, 317, 572, 1087, 1385, 1806, 2409, 4740, 4863
BspANI GGCC 8 cut(s) 838, 1206, 1331, 1683, 3567, 3678, 4829, 4947
BspCNI CTCAG 9 cut(s) 1318, 1389, 1789, 1901, 2461, 2566, 3271, 3562, 4103
BspDI ATCGAT 1 cut(s) 2484
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 7 cut(s) 801, 1171, 1933, 2442, 3337, 4171, 4585
BspLI GGNNCC 7 cut(s) 126, 1398, 1631, 2352, 3102, 3543, 5081
BspMAI CTGCAG 1 cut(s) 3516
BspMI ACCTGC 3 cut(s) 2098, 3505, 4832
BspOI GCTAGC 2 cut(s) 2840, 4112
BspQI GCTCTTC 2 cut(s) 620, 1583
BspT104I TTCGAA 1 cut(s) 4920
BspT107I GGYRCC 1 cut(s) 5079
BspTI CTTAAG 1 cut(s) 1103
BspTNI GGTCTC 2 cut(s) 3872, 4787
BsrDI GCAATG 2 cut(s) 456, 970
BsrGI TGTACA 3 cut(s) 1258, 3690, 3928
BsrI ACTGG 7 cut(s) 866, 1659, 2327, 3600, 3658, 4414, 4525
BssECI CCNNGG 7 cut(s) 315, 537, 561, 942, 2226, 2643, 2929
BssSI CACGAG 2 cut(s) 2298, 3236
BssT1I CCWWGG 5 cut(s) 561, 942, 2226, 2643, 2929
Bst2BI CACGAG 2 cut(s) 2298, 3236
Bst2UI CCWGG 6 cut(s) 373, 2585, 4062, 4202, 4944, 4979
Bst6I CTCTTC 7 cut(s) 298, 620, 1342, 1583, 2460, 2665, 4926
BstAFI CTTAAG 1 cut(s) 1103
BstAPI GCANNNNNTGC 2 cut(s) 2182, 4118
BstAUI TGTACA 3 cut(s) 1258, 3690, 3928
BstBI TTCGAA 1 cut(s) 4920
BstC8I GCNNGC 8 cut(s) 558, 2838, 3491, 3495, 3838, 3842, 4110, 5058
BstDSI CCRYGG 3 cut(s) 315, 537, 2929
BstEII GGTNACC 1 cut(s) 2303
BstENI CCTNNNNNAGG 1 cut(s) 3622
BstFNI CGCG 1 cut(s) 317
BstH2I RGCGCY 4 cut(s) 3320, 3346, 3847, 4109
BstHHI GCGC 5 cut(s) 3319, 3345, 3846, 4108, 4996
BstMAI GTCTC 7 cut(s) 51, 1724, 1931, 2465, 3448, 3872, 4787
BstNI CCWGG 6 cut(s) 373, 2585, 4062, 4202, 4944, 4979
BstNSI RCATGY 5 cut(s) 1069, 1597, 1701, 3260, 4890
BstPI GGTNACC 1 cut(s) 2303
BstSCI CCNGG 7 cut(s) 371, 2583, 2628, 4060, 4200, 4942, 4977
BstSFI CTRYAG 4 cut(s) 2733, 2893, 3512, 4677
BstSLI GKGCMC 1 cut(s) 4544
BstUI CGCG 1 cut(s) 317
BstV2I GAAGAC 4 cut(s) 294, 455, 4036, 4815
BstXI CCANNNNNNTGG 2 cut(s) 977, 4201
Bsu15I ATCGAT 1 cut(s) 2484
BsuRI GGCC 8 cut(s) 838, 1206, 1331, 1683, 3567, 3678, 4829, 4947
BsuTUI ATCGAT 1 cut(s) 2484
BtgI CCRYGG 3 cut(s) 315, 537, 2929
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 6 cut(s) 199, 468, 709, 960, 1960, 3754
BveI ACCTGC 3 cut(s) 2098, 3505, 4832
Cac8I GCNNGC 8 cut(s) 558, 2838, 3491, 3495, 3838, 3842, 4110, 5058
CaiI CAGNNNCTG 4 cut(s) 1733, 2144, 2757, 4514
CciI TCATGA 7 cut(s) 801, 1171, 1933, 2442, 3337, 4171, 4585
CfoI GCGC 5 cut(s) 3319, 3345, 3846, 4108, 4996
Cfr13I GGNCC 5 cut(s) 124, 1330, 4238, 4847, 4940
Cfr42I CCGCGG 1 cut(s) 318
ClaI ATCGAT 1 cut(s) 2484
CseI GACGC 1 cut(s) 3465
CsiI ACCWGGT 1 cut(s) 4060
CspCI CAANNNNNGTGG 2 cut(s) 1651, 1686
DraI TTTAAA 3 cut(s) 1843, 2338, 2832
DraIII CACNNNGTG 1 cut(s) 2303
DriI GACNNNNNGTC 2 cut(s) 1701, 2248
EaeI YGGCCR 1 cut(s) 3565
Eam1104I CTCTTC 7 cut(s) 298, 620, 1342, 1583, 2460, 2665, 4926
Eam1105I GACNNNNNGTC 2 cut(s) 1701, 2248
EarI CTCTTC 7 cut(s) 298, 620, 1342, 1583, 2460, 2665, 4926
Ecl136II GAGCTC 1 cut(s) 1645
Eco130I CCWWGG 5 cut(s) 561, 942, 2226, 2643, 2929
Eco147I AGGCCT 1 cut(s) 1683
Eco24I GRGCYC 3 cut(s) 1647, 2825, 4291
Eco31I GGTCTC 2 cut(s) 3872, 4787
Eco32I GATATC 2 cut(s) 2482, 2923
Eco47I GGWCC 4 cut(s) 124, 4238, 4847, 4940
Eco53kI GAGCTC 1 cut(s) 1645
Eco57I CTGAAG 8 cut(s) 1607, 2618, 3309, 3797, 4254, 4361, 4598, 4646
Eco88I CYCGRG 1 cut(s) 3311
Eco91I GGTNACC 1 cut(s) 2303
EcoICRI GAGCTC 1 cut(s) 1645
EcoNI CCTNNNNNAGG 1 cut(s) 3622
EcoO109I RGGNCCY 2 cut(s) 124, 1330
EcoO65I GGTNACC 1 cut(s) 2303
EcoRI GAATTC 1 cut(s) 2546
EcoRII CCWGG 6 cut(s) 371, 2583, 4060, 4200, 4942, 4977
EcoRV GATATC 2 cut(s) 2482, 2923
EcoT14I CCWWGG 5 cut(s) 561, 942, 2226, 2643, 2929
EcoT22I ATGCAT 5 cut(s) 1039, 1366, 1520, 3219, 3382
EcoT38I GRGCYC 3 cut(s) 1647, 2825, 4291
ErhI CCWWGG 5 cut(s) 561, 942, 2226, 2643, 2929
Esp3I CGTCTC 1 cut(s) 3448
FalI AAGNNNNNCTT 8 cut(s) 1668, 1700, 2861, 2893, 3909, 3941, 4800, 4832
FaqI GGGAC 1 cut(s) 486
FauI CCCGC 2 cut(s) 2416, 4733
FbaI TGATCA 1 cut(s) 3529
FblI GTMKAC 3 cut(s) 3519, 3922, 4965
FriOI GRGCYC 3 cut(s) 1647, 2825, 4291
GlaI GCGC 5 cut(s) 3318, 3344, 3845, 4107, 4995
GsuI CTGGAG 2 cut(s) 849, 1091
HaeII RGCGCY 4 cut(s) 3320, 3346, 3847, 4109
HaeIII GGCC 8 cut(s) 838, 1206, 1331, 1683, 3567, 3678, 4829, 4947
HapII CCGG 2 cut(s) 2630, 4355
HgaI GACGC 1 cut(s) 3465
HhaI GCGC 5 cut(s) 3319, 3345, 3846, 4108, 4996
Hin6I GCGC 5 cut(s) 3317, 3343, 3844, 4106, 4994
HinP1I GCGC 5 cut(s) 3317, 3343, 3844, 4106, 4994
HincII GTYRAC 7 cut(s) 16, 583, 2053, 3447, 3472, 3745, 4067
HindII GTYRAC 7 cut(s) 16, 583, 2053, 3447, 3472, 3745, 4067
HindIII AAGCTT 1 cut(s) 41
HpaI GTTAAC 1 cut(s) 3472
HpaII CCGG 2 cut(s) 2630, 4355
Hpy99I CGWCG 2 cut(s) 209, 4450
HpyCH4IV ACGT 4 cut(s) 417, 3465, 4707, 4767
HpySE526I ACGT 4 cut(s) 417, 3465, 4707, 4767
HspAI GCGC 5 cut(s) 3317, 3343, 3844, 4106, 4994
Ksp22I TGATCA 1 cut(s) 3529
KspAI GTTAAC 1 cut(s) 3472
KspI CCGCGG 1 cut(s) 318
LguI GCTCTTC 2 cut(s) 620, 1583
LmnI GCTCC 7 cut(s) 388, 535, 1214, 1943, 2104, 2620, 3731
MabI ACCWGGT 1 cut(s) 4060
MaeII ACGT 4 cut(s) 417, 3465, 4707, 4767
MfeI CAATTG 3 cut(s) 1238, 1424, 3702
MhlI GDGCHC 6 cut(s) 1647, 1768, 2825, 4291, 4544, 5058
MlsI TGGCCA 1 cut(s) 3567
MluNI TGGCCA 1 cut(s) 3567
MlyI GAGTC 8 cut(s) 445, 569, 673, 2651, 2776, 3442, 3653, 4166
MmeI TCCRAC 6 cut(s) 273, 1549, 3504, 4505, 4593, 4883
Mox20I TGGCCA 1 cut(s) 3567
Mph1103I ATGCAT 5 cut(s) 1039, 1366, 1520, 3219, 3382
MroXI GAANNNNTTC 3 cut(s) 809, 2016, 4958
MscI TGGCCA 1 cut(s) 3567
MslI CAYNNNNRTG 7 cut(s) 144, 237, 461, 722, 789, 1513, 5011
Msp20I TGGCCA 1 cut(s) 3567
MspA1I CMGCKG 6 cut(s) 317, 973, 1087, 1806, 2411, 4561
MspCI CTTAAG 1 cut(s) 1103
MspI CCGG 2 cut(s) 2630, 4355
MspR9I CCNGG 7 cut(s) 373, 2585, 2630, 4062, 4202, 4944, 4979
MssI GTTTAAAC 1 cut(s) 2338
MunI CAATTG 3 cut(s) 1238, 1424, 3702
Mva1269I GAATGC 5 cut(s) 1366, 1776, 3090, 3380, 5089
MvaI CCWGG 6 cut(s) 373, 2585, 4062, 4202, 4944, 4979
MvnI CGCG 1 cut(s) 317
NciI CCSGG 1 cut(s) 2630
NcoI CCATGG 1 cut(s) 2929
NheI GCTAGC 2 cut(s) 2836, 4108
NlaIV GGNNCC 7 cut(s) 126, 1398, 1631, 2352, 3102, 3543, 5081
NsiI ATGCAT 5 cut(s) 1039, 1366, 1520, 3219, 3382
NspI RCATGY 5 cut(s) 1069, 1597, 1701, 3260, 4890
NspV TTCGAA 1 cut(s) 4920
OliI CACNNNNGTG 1 cut(s) 144
PaeR7I CTCGAG 1 cut(s) 3311
PagI TCATGA 7 cut(s) 801, 1171, 1933, 2442, 3337, 4171, 4585
PaqCI CACCTGC 1 cut(s) 4832
PceI AGGCCT 1 cut(s) 1683
PciI ACATGT 2 cut(s) 3256, 4886
PciSI GCTCTTC 2 cut(s) 620, 1583
PctI GAATGC 5 cut(s) 1366, 1776, 3090, 3380, 5089
PdmI GAANNNNTTC 3 cut(s) 809, 2016, 4958
PflFI GACNNNGTC 2 cut(s) 578, 4779
PflMI CCANNNNNTGG 3 cut(s) 872, 3985, 4612
PfoI TCCNGGA 3 cut(s) 2583, 2628, 4977
PleI GAGTC 8 cut(s) 445, 569, 673, 2651, 2776, 3442, 3652, 4165
PmeI GTTTAAAC 1 cut(s) 2338
PpsI GAGTC 8 cut(s) 445, 569, 673, 2651, 2776, 3442, 3652, 4165
PpuMI RGGWCCY 1 cut(s) 124
PscI ACATGT 2 cut(s) 3256, 4886
PshBI ATTAAT 2 cut(s) 915, 4655
Psp124BI GAGCTC 1 cut(s) 1647
Psp1406I AACGTT 2 cut(s) 417, 3465
Psp5II RGGWCCY 1 cut(s) 124
Psp6I CCWGG 6 cut(s) 371, 2583, 4060, 4200, 4942, 4977
PspEI GGTNACC 1 cut(s) 2303
PspGI CCWGG 6 cut(s) 371, 2583, 4060, 4200, 4942, 4977
PspN4I GGNNCC 7 cut(s) 126, 1398, 1631, 2352, 3102, 3543, 5081
PspPI GGNCC 5 cut(s) 124, 1330, 4238, 4847, 4940
PspPPI RGGWCCY 1 cut(s) 124
PspXI VCTCGAGB 1 cut(s) 3311
PstI CTGCAG 1 cut(s) 3516
PstNI CAGNNNCTG 4 cut(s) 1733, 2144, 2757, 4514
PsyI GACNNNGTC 2 cut(s) 578, 4779
PvuII CAGCTG 2 cut(s) 973, 4561
RseI CAYNNNNRTG 7 cut(s) 144, 237, 461, 722, 789, 1513, 5011
SacI GAGCTC 1 cut(s) 1647
SacII CCGCGG 1 cut(s) 318
SapI GCTCTTC 2 cut(s) 620, 1583
Sau96I GGNCC 5 cut(s) 124, 1330, 4238, 4847, 4940
SchI GAGTC 8 cut(s) 445, 569, 673, 2651, 2776, 3442, 3653, 4166
ScrFI CCNGG 7 cut(s) 373, 2585, 2630, 4062, 4202, 4944, 4979
SduI GDGCHC 6 cut(s) 1647, 1768, 2825, 4291, 4544, 5058
SexAI ACCWGGT 1 cut(s) 4060
SfcI CTRYAG 4 cut(s) 2733, 2893, 3512, 4677
Sfr274I CTCGAG 1 cut(s) 3311
Sfr303I CCGCGG 1 cut(s) 318
SfuI TTCGAA 1 cut(s) 4920
SgrBI CCGCGG 1 cut(s) 318
SinI GGWCC 4 cut(s) 124, 4238, 4847, 4940
SlaI CTCGAG 1 cut(s) 3311
SmiMI CAYNNNNRTG 7 cut(s) 144, 237, 461, 722, 789, 1513, 5011
SmlI CTYRAG 7 cut(s) 19, 1103, 3014, 3311, 3362, 3386, 5084
SmoI CTYRAG 7 cut(s) 19, 1103, 3014, 3311, 3362, 3386, 5084
SpeI ACTAGT 1 cut(s) 143
SseBI AGGCCT 1 cut(s) 1683
SsiI CCGC 9 cut(s) 315, 317, 572, 1087, 1385, 1806, 2409, 4740, 4863
SspI AATATT 5 cut(s) 747, 846, 1479, 1988, 2857
SstI GAGCTC 1 cut(s) 1647
StuI AGGCCT 1 cut(s) 1683
StyD4I CCNGG 7 cut(s) 371, 2583, 2628, 4060, 4200, 4942, 4977
StyI CCWWGG 5 cut(s) 561, 942, 2226, 2643, 2929
TaiI ACGT 4 cut(s) 420, 3468, 4710, 4770
TaqII GACCGA 2 cut(s) 1008, 4088
TatI WGTACW 5 cut(s) 1258, 2197, 3690, 3817, 3928
TauI GCSGC 2 cut(s) 320, 1388
TscAI CASTG 6 cut(s) 199, 468, 716, 960, 1960, 3754
TspGWI ACGGA 5 cut(s) 341, 422, 1703, 3515, 4599
TspRI CASTG 6 cut(s) 199, 468, 716, 960, 1960, 3754
Tth111I GACNNNGTC 2 cut(s) 578, 4779
Van91I CCANNNNNTGG 3 cut(s) 872, 3985, 4612
Vha464I CTTAAG 1 cut(s) 1103
VpaK11BI GGWCC 4 cut(s) 124, 4238, 4847, 4940
VspI ATTAAT 2 cut(s) 915, 4655
XagI CCTNNNNNAGG 1 cut(s) 3622
XbaI TCTAGA 4 cut(s) 1409, 1867, 2663, 4348
XceI RCATGY 5 cut(s) 1069, 1597, 1701, 3260, 4890
XcmI CCANNNNNNNNNTGG 4 cut(s) 541, 2936, 2977, 5005
XhoI CTCGAG 1 cut(s) 3311
XmiI GTMKAC 3 cut(s) 3519, 3922, 4965
XmnI GAANNNNTTC 3 cut(s) 809, 2016, 4958
Zsp2I ATGCAT 5 cut(s) 1039, 1366, 1520, 3219, 3382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.