Rh2BG017500

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004795
Physical Location & Seq
Reverse (-)
1275076 .. 1288497
13422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG017500.1

Sequence Viewer

Length: 4917 bp
ATGGACGACGCGCCGCCCGATACGGGTCGGGCTGTGGAGTCGGGTGATGGAGCTCCGAGGCCGGAGAAGGATCCGAGGAAGGTTGCCCGGAGGTATCAATTGGAGCTGTGTCAGAAAGCTGTCGAGGAGAACATAATTGTCTATTTGGAAACTGGCTGTGGAAAGACCCACATAGCAGTGTTGCTTATCTATGAGCTGCGCGAGTTGATAATGAAACCTCGTAAGAACATATGTGTGTTTCTTGCGCCTACGGTGGCGCTTGTTCAACAGCAAGCAAGAGTTATAGAAGATTCCCTTGATCTCAAGGTTGGGACCTATTGTGGAAGCTCTAAGCATTTGAAAACTCACCAAGACTGGGAAAAAGAGATGGAACAATATGAGGTTTTTGTCATGACGCCTCAAATACTGTTACGTAACTTATACCACCGTATTATTAAGATGGAGTTGATTGCATTGCTGATTTTTGATGAGTGTCATCACGCTCAAATGAAAAGCAACCATCCTTATGCAGAAATTATGAAATTTTACAAGAGTGATGCCACTAAACTTCCTCGTATATTTGGCATGACTGCATCTCCAGTTGTAGGTAAAGGCACTTCTAGTCAATCGAATTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATACGGTTGAAGACAAGACAGAATTGCATTGTTCTGTTGTTTCTCCTGTTATCAATGTCCATCGTTATGGTCCTGTTATTAGTGGCACATCCAGCCCCTGCATGACTCTTTCTAGAAAACTTGACCAGCTGAAACGCCAGTGTATAGCAGAACTTAGTAAGAAGACAAGTGACTACCAAAGTCTAAGGAGTACAAAGAAAACGTTAAACAGAGTGCATGACAGTATAATATTTTGCTTGGAAAGTCTTGGCCTCTGGGGTGCGTTACAAGCTAGACATATTCTTTCGAGTGGCGATAACTTTGAACGAAATGCATTAATAGAGGAAGGCAATAATGATATAGAGGCAGAAGGAAATAATAGTGATGACTCTGTATGTGCGGAATATCTGGCTCAGGCAGCTGACATCATTCGTACTGACTGTGGGAAAGATGCTGTTGCATCTGGTCTATCTTGTGTAGAGATCTTAAAGGAGCCATTCTTCTCAAGTAAGGTTTTACGTCTTATTGGAATTCTTTCATCCTTCAGGTTGCAGCAGGATATGAAATGCATAATTTTTGTCAATAGGATTATTACTGCAAGATCCTTGTCCTACATACTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCGAAACTGAAGAGTATGTCAAGGAAGACGATGAAGATCATTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGACTAGATATTCAGACTTGCTGCCTTGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGGCTCAGTCTGAATATATATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTGGATTTAATAGAAAACTTCAGGAAAGATGAAGAGCGAATGAACATGGAAGTTGCTTTCAGAACATCCAGTGAGACTTTTCTTAGTCCTGAGGAACGAACATATAAAGTTGCTTCTACTGGAGCTTCCGTCACTTCTGGATATAGCATCTCATTGTTACACCAGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCACTCCAGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCAGATAATTTTACCTTCCAATGCTCCAATGCATCAAATTGTCAGTACACCACAATCTTCGATGGAAGATGCCAAAAAAGATGCATGTCTAAAAGCCATTGAAGAATTGCATAAATTGGGTGCCTTAAGTGACTTTCTCTTGCCACAGCAAGACAATGCAAATGTGGAGGAGTCCATGCTAGACTCTTCTGATTATGACAGCAATGAAGATGAAGACTTACGAGAGGAACTACATGAAATGCTGGTTCCTGCTGTCCTAAAAGAGTCATGGAATAAGTTGGAGGATCTTGTCACCCTCAGCGCTTACTATTTTCAATTTGATCCTGAACCTAATGACAGAATCTATAGAAGTTTTGGTCTATTTGTCAAAGCACCTCTTCCAGCAGAGGCCGAAACAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCAGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGCTCATTCTGGACCGATCAGAATTTGTTTCTGAATTTGTTCCCCTGGGAAACTATGATTTTAGCAGATCGAGCACATCAACCTTCTACCTATTGCTTCCAGTAACTTTGGGTGAAAATGATAAGATAAGCATAGATTGGAGGATTATCAAAAAATGTTTATCATCTCCAATATTCAGGGGTCCAGGAGATGCTATGGACAGTAAAATTATATCTTTGGGTATTCGACTTGCATCTGGTTATAGAAGCATAAGTGAAGTTGAGGATAGTTTAGTTTATGTCCCATATAAGAAGGCATTTTACTTCATCACTAATGTTGCTCGAGAAAGAAATGCCTACAGCCAATATAAAGAAAAGTCAGATGCTTTAACCTATGTGGATCATCTAAGTAAAAAGTTTGACATTTCTCTTAAATACCCTGAGCAACATCTTCTGTGTGCAAAACCAGTTTTCAATTTGCACAACTTGCTACACAATCGTAGGCAAGAAGATTCAGAAGCACAGCAACTAGATGAGTATTATATATATGTGCCTCCTGAGCTTTGCGAGCTGAAGGTAATTGGCTTTTCAAAAGATATTGGGAGTTCGGTATCTCTGTTACCATCAATCATGCACCGTCTGGAAAACTTGCTTGTCGCCATCGAACTGAAGCATGTATTATGCACTTCATTCCCCGAAGGAGCTGAGGTTACTTCGCAGAGAGTTCTAGAAGCCCTCACAACTGAAAAGTGCCAGGAGCGGATTTCCCTTGAAAGGCTTGAAATACTAGGAGATGCTTTCCTCAAATTTGCAGTTGGAAGGCACTTTTTCCTTTTGCATGCTTCACTTGATGAAGGTGAACTAACAAGGAAACGTTCAAATGTGGTAAACAATTCCAATTTATTGAAGCTTGCTACTAGAAGCAACTTACAGGTATACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTCGCTTTAGGCCGCCCTTGCAAAAATATTTGTGACCAGGAGACAATAGGATCTCTTGATTCTCAAGATTTAAGCAGTGCAGTAAAACAGTCACATGCTCGTCACGTCAGATGTAGTAAGGGTCACCATTGGTTACATAAGAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCGGCAACTGCATTTCTCAGATGGATTGGCATAAAAGTAAACTTTGAAGCATCAGAAGTTACAAAAGTATGCACAGCAAGCAGCAGATACGTTTCACTTGCTGCTTGCATAGACATTGCGGCCCTTGAAAATTCATTAGGGTATCAGTTTCTCCACAGAGGTTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAACATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTGTATCCAAAGTTAAAGCCTGGCCATTTGACAGATTTGAGATCAATTGCTGTGAATAATAAAGCCTTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTGCTCTCTGACACCAGTACCCTTTCTGAGGCTATAAAGACTTATGTGAACTTCATTGAGACATCTGCATCAGATAGTAGTCTGGTTGATGGGCCGAAATGCCCCAAGGCTCTGGGCGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGGTTTGACTTAAACCTTGTTTGGAAGATAATGCTATCCTTTCTGGAGCCTGTCATGAGCTTTTCCAACATTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGGTTCTTAAGTTTTTATCATCAAAGAAGGGTAAAACATTTTCAGTTGAGGCAACAGTGAATGGGAATAATGTTTCTGCAACTGCTTCCTCAACTGCCTTGAACAAAAAGGAGGCTATTAGAATTTCTGCTCAGTTAATTTTCGAAACGTTGAAGGCTCAAGGCAATATACCAAAGTCTAAGTTGTCTTTGGAGGAAGTCTTGAAGTCAAGCTCCAAGTTGGAACCCAAACTTATCGGATATGATGAAACACCAATAGATGTTACAGCTCTTGATATTATTGGATTTGATAAGTTAAATGTACAAGAGGCTTCTAGCAGCAGTTCCAACTCAGATGTGCAGTCTATGAGTGAAGTTAGCAGTGGTAACTCCTCCTGCATAAAACGTTTACAACAACCCCCAGCTTCACTTGGAGAAGTCAAAATGGACGGCACTAATGCCTGTAACAGTCGTAGCTCTGATGCTATCTCACAAACCGGAGGTGTGCCACACAAATTAACAGCAAAATCTCGTTTGCATGAAACCTGTGTTGCTAACTACTGGGAACCCCCTGAATTTGAATGTTGCAAAGAGGAAGGACCAGGCCACATAAAATCGTTCACTTTCAAGGTTACAGTGAAGATAGATGATGTTTCGGATACACTTTTGGAGGCCTTTGGAGAACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGGGCACTTTGGTACTTGGAAAAGAAAGGTTACATAGAGGAGAGTGATCAATCAGAGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1638

Amino Acids

183.29

Weight (kDa)

5.88

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 30 - 193 6.4e-17 Type III restriction enzyme, res subunit
DEAD PF00270 31 - 193 1.3e-18 DEAD/DEAH box helicase
Helicase_C PF00271 388 - 501 1.2e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 568 - 653 1.8e-23 Dicer dimerisation domain
PAZ PF02170 861 - 981 1.3e-08 PAZ domain
Ribonucleas_3_3 PF14622 1025 - 1083 5.5e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1026 - 1158 4.1e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1214 - 1345 7.9e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1234 - 1343 3.6e-21 Ribonuclease III domain
DND1_DSRM PF14709 1549 - 1624 2.6e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1550 - 1620 4.3e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1925, 2254
AccBSI CCGCTC 1 cut(s) 3064
AccI GTMKAC 1 cut(s) 3240
AccII CGCG 2 cut(s) 11, 201
AciI CCGC 8 cut(s) 14, 1040, 3064, 3289, 3471, 3482, 3599, 4847
AclI AACGTT 4 cut(s) 863, 3178, 4295, 4531
AclWI GGATC 9 cut(s) 65, 78, 1236, 2097, 2120, 2712, 3258, 3334, 3846
AcoI YGGCCR 1 cut(s) 3784
AcuI CTGAAG 5 cut(s) 1168, 1343, 1561, 2897, 2993
AcyI GRCGYC 1 cut(s) 395
AfaI GTAC 8 cut(s) 853, 1075, 1852, 3663, 3886, 4449, 4816, 4865
AfeI AGCGCT 1 cut(s) 2107
AfiI CCNNNNNNNGG 5 cut(s) 23, 355, 584, 3078, 3895
AflII CTTAAG 3 cut(s) 1268, 1930, 4154
AjiI CACGTC 1 cut(s) 3382
AjnI CCWGG 6 cut(s) 2370, 2509, 3057, 3312, 3781, 4726
AjuI GAANNNNNNNTTGG 2 cut(s) 3432, 3464
Alw21I GWGCWC 3 cut(s) 55, 1504, 2402
Alw26I GTCTC 3 cut(s) 1625, 3311, 3920
Alw44I GTGCAC 1 cut(s) 1500
AlwI GGATC 9 cut(s) 65, 78, 1236, 2097, 2120, 2712, 3258, 3334, 3846
AlwNI CAGNNNCTG 2 cut(s) 759, 1469
Ama87I CYCGRG 1 cut(s) 2646
Aor51HI AGCGCT 1 cut(s) 2107
AoxI GGCC 9 cut(s) 59, 910, 2193, 3286, 3600, 3784, 3959, 4729, 4797
ApaLI GTGCAC 1 cut(s) 1500
AseI ATTAAT 1 cut(s) 977
Asp700I GAANNNNTTC 2 cut(s) 1174, 2364
AspLEI GCGC 5 cut(s) 13, 201, 247, 259, 2108
AspS9I GGNCC 7 cut(s) 312, 731, 2338, 2507, 3601, 3959, 4724
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 9 cut(s) 56, 338, 1421, 1796, 2089, 2450, 3173, 3392, 3736
AsuII TTCGAA 2 cut(s) 1730, 4290
AsuNHI GCTAGC 1 cut(s) 1475
AvaI CYCGRG 1 cut(s) 2646
AvaII GGWCC 5 cut(s) 312, 731, 2338, 2507, 4724
AxyI CCTNAGG 1 cut(s) 1647
BaeGI GKGCMC 2 cut(s) 1504, 4858
BaeI ACNNNNGTAYC 2 cut(s) 3868, 3901
BalI TGGCCA 1 cut(s) 3786
BamHI GGATCC 1 cut(s) 70
BanI GGYRCC 2 cut(s) 1925, 2254
BanII GRGCYC 1 cut(s) 55
BarI GAAGNNNNNNTAC 4 cut(s) 580, 612, 2609, 2641
BauI CACGAG 1 cut(s) 1491
BbsI GAAGAC 4 cut(s) 678, 830, 1346, 2025
Bbv12I GWGCWC 3 cut(s) 55, 1504, 2402
BbvCI CCTCAGC 2 cut(s) 2102, 3009
BceAI ACGGC 1 cut(s) 4591
BciT130I CCWGG 6 cut(s) 2372, 2511, 3059, 3314, 3783, 4728
BciVI GTATCC 2 cut(s) 3777, 4777
BclI TGATCA 2 cut(s) 3741, 4897
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 3 cut(s) 1625, 3311, 3920
BfmI CTRYAG 2 cut(s) 2149, 2662
BfoI RGCGCY 2 cut(s) 260, 2109
BfrI CTTAAG 3 cut(s) 1268, 1930, 4154
BfuI GTATCC 2 cut(s) 3777, 4777
BglII AGATCT 3 cut(s) 1122, 2234, 2289
Bme1390I CCNGG 7 cut(s) 88, 2372, 2511, 3059, 3314, 3783, 4728
Bme18I GGWCC 5 cut(s) 312, 731, 2338, 2507, 4724
BmeT110I CYCGRG 1 cut(s) 2646
BmgBI CACGTC 1 cut(s) 3382
BmgT120I GGNCC 7 cut(s) 312, 731, 2338, 2507, 3601, 3959, 4724
BmrFI CCNGG 7 cut(s) 88, 2372, 2511, 3059, 3314, 3783, 4728
BmrI ACTGGG 2 cut(s) 364, 4696
BmtI GCTAGC 1 cut(s) 1479
BmuI ACTGGG 2 cut(s) 364, 4696
BpiI GAAGAC 4 cut(s) 678, 830, 1346, 2025
BpmI CTGGAG 5 cut(s) 561, 1395, 1698, 1749, 4091
Bpu10I CCTNAGC 6 cut(s) 654, 1053, 2102, 2745, 2862, 3009
Bpu14I TTCGAA 2 cut(s) 1730, 4290
BpuEI CTTGAG 5 cut(s) 287, 1129, 2309, 3324, 4290
BpuMI CCSGG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 413
BsaBI GATNNNNATC 2 cut(s) 1349, 2217
BsaHI GRCGYC 1 cut(s) 395
BsaJI CCNNGG 5 cut(s) 56, 74, 2370, 2371, 3972
BsaWI WCCGGW 1 cut(s) 4622
BsaXI ACNNNNNCTCC 8 cut(s) 559, 589, 1671, 1701, 2997, 3027, 3711, 3741
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 355, 584, 3078, 3895
Bse21I CCTNAGG 1 cut(s) 1647
Bse3DI GCAATG 3 cut(s) 452, 2014, 3594
Bse8I GATNNNNATC 2 cut(s) 1349, 2217
BseBI CCWGG 6 cut(s) 2372, 2511, 3059, 3314, 3783, 4728
BseDI CCNNGG 5 cut(s) 56, 74, 2370, 2371, 3972
BseGI GGATG 6 cut(s) 499, 749, 1178, 1512, 1622, 3664
BseJI GATNNNNATC 2 cut(s) 1349, 2217
BseLI CCNNNNNNNGG 5 cut(s) 23, 355, 584, 3078, 3895
BseMI GCAATG 3 cut(s) 452, 2014, 3594
BseRI GAGGAG 5 cut(s) 140, 1988, 3702, 4507, 4904
BseSI GKGCMC 2 cut(s) 1504, 4858
BseYI CCCAGC 1 cut(s) 4546
BsgI GTGCAG 2 cut(s) 3375, 4505
Bsh1236I CGCG 2 cut(s) 11, 201
BshFI GGCC 9 cut(s) 61, 912, 2195, 3288, 3602, 3786, 3961, 4731, 4799
BshNI GGYRCC 2 cut(s) 1925, 2254
BsiHKAI GWGCWC 3 cut(s) 55, 1504, 2402
BsiHKCI CYCGRG 1 cut(s) 2646
BsiSI CCGG 3 cut(s) 62, 88, 4623
BsiWI CGTACG 1 cut(s) 4814
BslFI GGGAC 4 cut(s) 325, 1431, 1742, 2591
BslI CCNNNNNNNGG 5 cut(s) 23, 355, 584, 3078, 3895
BsmAI GTCTC 3 cut(s) 1625, 3311, 3920
BsmFI GGGAC 4 cut(s) 325, 1431, 1742, 2591
BsmI GAATGC 1 cut(s) 3455
BsnI GGCC 9 cut(s) 61, 912, 2195, 3288, 3602, 3786, 3961, 4731, 4799
BsoBI CYCGRG 1 cut(s) 2646
Bsp119I TTCGAA 2 cut(s) 1730, 4290
Bsp1286I GDGCHC 4 cut(s) 55, 1504, 2402, 4858
Bsp1407I TGTACA 2 cut(s) 3661, 4447
BspACI CCGC 8 cut(s) 14, 1040, 3064, 3289, 3471, 3482, 3599, 4847
BspANI GGCC 9 cut(s) 61, 912, 2195, 3288, 3602, 3786, 3961, 4731, 4799
BspFNI CGCG 2 cut(s) 11, 201
BspHI TCATGA 2 cut(s) 390, 4080
BspOI GCTAGC 1 cut(s) 1479
BspPI GGATC 9 cut(s) 65, 78, 1236, 2097, 2120, 2712, 3258, 3334, 3846
BspQI GCTCTTC 1 cut(s) 1584
BspT104I TTCGAA 2 cut(s) 1730, 4290
BspT107I GGYRCC 2 cut(s) 1925, 2254
BspTI CTTAAG 3 cut(s) 1268, 1930, 4154
BsrBI CCGCTC 1 cut(s) 3064
BsrDI GCAATG 3 cut(s) 452, 2014, 3594
BsrGI TGTACA 2 cut(s) 3661, 4447
BssECI CCNNGG 5 cut(s) 56, 74, 2370, 2371, 3972
BssNAI GTATAC 1 cut(s) 3241
BssNI GRCGYC 1 cut(s) 395
BssSI CACGAG 1 cut(s) 1491
BssT1I CCWWGG 1 cut(s) 3972
Bst1107I GTATAC 1 cut(s) 3241
Bst2BI CACGAG 1 cut(s) 1491
Bst2UI CCWGG 6 cut(s) 2372, 2511, 3059, 3314, 3783, 4728
Bst6I CTCTTC 5 cut(s) 1319, 1407, 1584, 1996, 2187
BstACI GRCGYC 1 cut(s) 395
BstAFI CTTAAG 3 cut(s) 1268, 1930, 4154
BstAPI GCANNNNNTGC 2 cut(s) 2791, 4841
BstAUI TGTACA 2 cut(s) 3661, 4447
BstBAI YACGTR 1 cut(s) 413
BstBI TTCGAA 2 cut(s) 1730, 4290
BstC8I GCNNGC 9 cut(s) 273, 1477, 2298, 2873, 3144, 3216, 3559, 3586, 4842
BstEII GGTNACC 1 cut(s) 3398
BstENI CCTNNNNNAGG 1 cut(s) 3893
BstF5I GGATG 6 cut(s) 499, 749, 1178, 1512, 1622, 3664
BstFNI CGCG 2 cut(s) 11, 201
BstH2I RGCGCY 2 cut(s) 260, 2109
BstHHI GCGC 5 cut(s) 13, 201, 247, 259, 2108
BstMAI GTCTC 3 cut(s) 1625, 3311, 3920
BstNI CCWGG 6 cut(s) 2372, 2511, 3059, 3314, 3783, 4728
BstNSI RCATGY 5 cut(s) 1893, 2981, 3146, 3374, 4844
BstPI GGTNACC 1 cut(s) 3398
BstSCI CCNGG 7 cut(s) 86, 2370, 2509, 3057, 3312, 3781, 4726
BstSFI CTRYAG 2 cut(s) 2149, 2662
BstSLI GKGCMC 2 cut(s) 1504, 4858
BstSNI TACGTA 1 cut(s) 413
BstUI CGCG 2 cut(s) 11, 201
BstV2I GAAGAC 4 cut(s) 678, 830, 1346, 2025
BstX2I RGATCY 7 cut(s) 70, 1122, 1241, 2089, 2234, 2289, 3326
BstXI CCANNNNNNTGG 2 cut(s) 728, 3979
BstYI RGATCY 7 cut(s) 70, 1122, 1241, 2089, 2234, 2289, 3326
BstZ17I GTATAC 1 cut(s) 3241
Bsu36I CCTNAGG 1 cut(s) 1647
BsuI GTATCC 2 cut(s) 3777, 4777
BsuRI GGCC 9 cut(s) 61, 912, 2195, 3288, 3602, 3786, 3961, 4731, 4799
BtrI CACGTC 1 cut(s) 3382
BtsCI GGATG 6 cut(s) 499, 749, 1178, 1512, 1622, 3664
BtsI GCAGTG 3 cut(s) 183, 3358, 4513
BtsIMutI CAGTG 9 cut(s) 183, 806, 1546, 1633, 3358, 3768, 4209, 4513, 4767
Cac8I GCNNGC 9 cut(s) 273, 1477, 2298, 2873, 3144, 3216, 3559, 3586, 4842
CaiI CAGNNNCTG 2 cut(s) 759, 1469
CciI TCATGA 2 cut(s) 390, 4080
CfoI GCGC 5 cut(s) 13, 201, 247, 259, 2108
Cfr13I GGNCC 7 cut(s) 312, 731, 2338, 2507, 3601, 3959, 4724
CseI GACGC 2 cut(s) 17, 403
Csp6I GTAC 8 cut(s) 852, 1074, 1851, 3662, 3885, 4448, 4815, 4864
CviQI GTAC 8 cut(s) 852, 1074, 1851, 3662, 3885, 4448, 4815, 4864
DraI TTTAAA 1 cut(s) 3478
EaeI YGGCCR 1 cut(s) 3784
Eam1104I CTCTTC 5 cut(s) 1319, 1407, 1584, 1996, 2187
EarI CTCTTC 5 cut(s) 1319, 1407, 1584, 1996, 2187
Ecl136II GAGCTC 1 cut(s) 53
Eco105I TACGTA 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 3972
Eco147I AGGCCT 1 cut(s) 4799
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 5 cut(s) 312, 731, 2338, 2507, 4724
Eco47III AGCGCT 1 cut(s) 2107
Eco53kI GAGCTC 1 cut(s) 53
Eco57I CTGAAG 5 cut(s) 1168, 1343, 1561, 2897, 2993
Eco81I CCTNAGG 1 cut(s) 1647
Eco88I CYCGRG 1 cut(s) 2646
Eco91I GGTNACC 1 cut(s) 3398
EcoICRI GAGCTC 1 cut(s) 53
EcoNI CCTNNNNNAGG 1 cut(s) 3893
EcoO109I RGGNCCY 1 cut(s) 312
EcoO65I GGTNACC 1 cut(s) 3398
EcoRI GAATTC 2 cut(s) 1170, 1769
EcoRII CCWGG 6 cut(s) 2370, 2509, 3057, 3312, 3781, 4726
EcoT14I CCWWGG 1 cut(s) 3972
EcoT22I ATGCAT 5 cut(s) 976, 1211, 1839, 1891, 4147
EcoT38I GRGCYC 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 3972
FalI AAGNNNNNCTT 6 cut(s) 279, 311, 2166, 2198, 4842, 4874
FaqI GGGAC 4 cut(s) 325, 1431, 1742, 2591
FauNDI CATATG 1 cut(s) 230
FbaI TGATCA 2 cut(s) 3741, 4897
FblI GTMKAC 1 cut(s) 3240
FokI GGATG 6 cut(s) 486, 736, 1165, 1519, 1609, 3651
FriOI GRGCYC 1 cut(s) 55
GlaI GCGC 5 cut(s) 12, 200, 246, 258, 2107
GsaI CCCAGC 1 cut(s) 4550
GsuI CTGGAG 5 cut(s) 561, 1395, 1698, 1749, 4091
HaeII RGCGCY 2 cut(s) 260, 2109
HaeIII GGCC 9 cut(s) 61, 912, 2195, 3288, 3602, 3786, 3961, 4731, 4799
HapII CCGG 3 cut(s) 62, 88, 4623
HgaI GACGC 2 cut(s) 17, 403
HhaI GCGC 5 cut(s) 13, 201, 247, 259, 2108
Hin1I GRCGYC 1 cut(s) 395
Hin6I GCGC 5 cut(s) 11, 199, 245, 257, 2106
HinP1I GCGC 5 cut(s) 11, 199, 245, 257, 2106
HincII GTYRAC 1 cut(s) 3466
HindII GTYRAC 1 cut(s) 3466
HindIII AAGCTT 2 cut(s) 1733, 3212
HpaII CCGG 3 cut(s) 62, 88, 4623
HphI GGTGA 9 cut(s) 56, 338, 1421, 1796, 2089, 2450, 3173, 3392, 3736
Hpy99I CGWCG 1 cut(s) 11
HpyCH4IV ACGT 8 cut(s) 412, 863, 1159, 3178, 3381, 3570, 4295, 4531
HpySE526I ACGT 8 cut(s) 412, 863, 1159, 3178, 3381, 3570, 4295, 4531
Hsp92I GRCGYC 1 cut(s) 395
HspAI GCGC 5 cut(s) 11, 199, 245, 257, 2106
Ksp22I TGATCA 2 cut(s) 3741, 4897
LguI GCTCTTC 1 cut(s) 1584
MaeII ACGT 8 cut(s) 412, 863, 1159, 3178, 3381, 3570, 4295, 4531
MbiI CCGCTC 1 cut(s) 3064
MfeI CAATTG 3 cut(s) 98, 3423, 3807
MflI RGATCY 7 cut(s) 70, 1122, 1241, 2089, 2234, 2289, 3326
MhlI GDGCHC 4 cut(s) 55, 1504, 2402, 4858
MlsI TGGCCA 1 cut(s) 3786
MluNI TGGCCA 1 cut(s) 3786
MlyI GAGTC 8 cut(s) 47, 760, 1022, 1317, 1982, 1985, 2078, 4004
MmeI TCCRAC 7 cut(s) 1539, 2064, 3100, 3430, 4116, 4347, 4497
Mox20I TGGCCA 1 cut(s) 3786
Mph1103I ATGCAT 5 cut(s) 976, 1211, 1839, 1891, 4147
MroXI GAANNNNTTC 2 cut(s) 1174, 2364
MscI TGGCCA 1 cut(s) 3786
MslI CAYNNNNRTG 4 cut(s) 176, 233, 504, 726
Msp20I TGGCCA 1 cut(s) 3786
MspA1I CMGCKG 4 cut(s) 790, 1061, 3471, 4847
MspCI CTTAAG 3 cut(s) 1268, 1930, 4154
MspI CCGG 3 cut(s) 62, 88, 4623
MspR9I CCNGG 7 cut(s) 88, 2372, 2511, 3059, 3314, 3783, 4728
MunI CAATTG 3 cut(s) 98, 3423, 3807
Mva1269I GAATGC 1 cut(s) 3455
MvaI CCWGG 6 cut(s) 2372, 2511, 3059, 3314, 3783, 4728
MvnI CGCG 2 cut(s) 11, 201
NciI CCSGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 230
NheI GCTAGC 1 cut(s) 1475
NmuCI GTSAC 9 cut(s) 830, 1488, 1687, 1934, 2095, 3308, 3366, 3377, 3398
NsiI ATGCAT 5 cut(s) 976, 1211, 1839, 1891, 4147
NspI RCATGY 5 cut(s) 1893, 2981, 3146, 3374, 4844
NspV TTCGAA 2 cut(s) 1730, 4290
PaeI GCATGC 2 cut(s) 3146, 4844
PaeR7I CTCGAG 1 cut(s) 2646
PagI TCATGA 2 cut(s) 390, 4080
PasI CCCWGGG 1 cut(s) 2371
PceI AGGCCT 1 cut(s) 4799
PciSI GCTCTTC 1 cut(s) 1584
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 3455
PdmI GAANNNNTTC 2 cut(s) 1174, 2364
PfeI GAWTC 6 cut(s) 290, 1273, 1384, 2145, 2816, 3335
Pfl23II CGTACG 1 cut(s) 4814
PfoI TCCNGGA 1 cut(s) 2509
PleI GAGTC 8 cut(s) 46, 760, 1022, 1316, 1982, 1984, 2077, 4003
PpsI GAGTC 8 cut(s) 46, 760, 1022, 1316, 1982, 1984, 2077, 4003
Ppu21I YACGTR 1 cut(s) 413
PpuMI RGGWCCY 1 cut(s) 312
PshBI ATTAAT 1 cut(s) 977
Psp124BI GAGCTC 1 cut(s) 55
Psp1406I AACGTT 4 cut(s) 863, 3178, 4295, 4531
Psp5II RGGWCCY 1 cut(s) 312
Psp6I CCWGG 6 cut(s) 2370, 2509, 3057, 3312, 3781, 4726
PspEI GGTNACC 1 cut(s) 3398
PspFI CCCAGC 1 cut(s) 4546
PspGI CCWGG 6 cut(s) 2370, 2509, 3057, 3312, 3781, 4726
PspLI CGTACG 1 cut(s) 4814
PspPI GGNCC 7 cut(s) 312, 731, 2338, 2507, 3601, 3959, 4724
PspPPI RGGWCCY 1 cut(s) 312
PstNI CAGNNNCTG 2 cut(s) 759, 1469
PsuI RGATCY 7 cut(s) 70, 1122, 1241, 2089, 2234, 2289, 3326
PvuII CAGCTG 2 cut(s) 790, 1061
RsaI GTAC 8 cut(s) 853, 1075, 1852, 3663, 3886, 4449, 4816, 4865
RsaNI GTAC 8 cut(s) 852, 1074, 1851, 3662, 3885, 4448, 4815, 4864
RseI CAYNNNNRTG 4 cut(s) 176, 233, 504, 726
SacI GAGCTC 1 cut(s) 55
SapI GCTCTTC 1 cut(s) 1584
Sau96I GGNCC 7 cut(s) 312, 731, 2338, 2507, 3601, 3959, 4724
SchI GAGTC 8 cut(s) 47, 760, 1022, 1317, 1982, 1985, 2078, 4004
ScrFI CCNGG 7 cut(s) 88, 2372, 2511, 3059, 3314, 3783, 4728
SduI GDGCHC 4 cut(s) 55, 1504, 2402, 4858
SfcI CTRYAG 2 cut(s) 2149, 2662
Sfr274I CTCGAG 1 cut(s) 2646
SfuI TTCGAA 2 cut(s) 1730, 4290
SinI GGWCC 5 cut(s) 312, 731, 2338, 2507, 4724
SlaI CTCGAG 1 cut(s) 2646
SmiMI CAYNNNNRTG 4 cut(s) 176, 233, 504, 726
SmlI CTYRAG 9 cut(s) 302, 1144, 1268, 1930, 2324, 2646, 3339, 4154, 4305
SmoI CTYRAG 9 cut(s) 302, 1144, 1268, 1930, 2324, 2646, 3339, 4154, 4305
SnaBI TACGTA 1 cut(s) 413
SphI GCATGC 2 cut(s) 3146, 4844
SseBI AGGCCT 1 cut(s) 4799
SsiI CCGC 8 cut(s) 14, 1040, 3064, 3289, 3471, 3482, 3599, 4847
SspI AATATT 3 cut(s) 891, 2499, 3304
SstI GAGCTC 1 cut(s) 55
StuI AGGCCT 1 cut(s) 4799
StyD4I CCNGG 7 cut(s) 86, 2370, 2509, 3057, 3312, 3781, 4726
StyI CCWWGG 1 cut(s) 3972
TaiI ACGT 8 cut(s) 415, 866, 1162, 3181, 3384, 3573, 4298, 4534
TaqII GACCGA 2 cut(s) 1359, 2355
TatI WGTACW 4 cut(s) 851, 1850, 3661, 4447
TauI GCSGC 4 cut(s) 16, 3291, 3485, 3602
TfiI GAWTC 6 cut(s) 290, 1273, 1384, 2145, 2816, 3335
TscAI CASTG 9 cut(s) 183, 806, 1546, 1633, 3358, 3768, 4209, 4513, 4767
TseFI GTSAC 9 cut(s) 830, 1488, 1687, 1934, 2095, 3308, 3366, 3377, 3398
Tsp45I GTSAC 9 cut(s) 830, 1488, 1687, 1934, 2095, 3308, 3366, 3377, 3398
TspGWI ACGGA 2 cut(s) 1519, 1675
TspRI CASTG 9 cut(s) 183, 806, 1546, 1633, 3358, 3768, 4209, 4513, 4767
Vha464I CTTAAG 3 cut(s) 1268, 1930, 4154
VneI GTGCAC 1 cut(s) 1500
VpaK11BI GGWCC 5 cut(s) 312, 731, 2338, 2507, 4724
VspI ATTAAT 1 cut(s) 977
XagI CCTNNNNNAGG 1 cut(s) 3893
XbaI TCTAGA 2 cut(s) 773, 3031
XceI RCATGY 5 cut(s) 1893, 2981, 3146, 3374, 4844
XhoI CTCGAG 1 cut(s) 2646
XmiI GTMKAC 1 cut(s) 3240
XmnI GAANNNNTTC 2 cut(s) 1174, 2364
Zsp2I ATGCAT 5 cut(s) 976, 1211, 1839, 1891, 4147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.