RchiOBHm_Chr2g0086421

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004795
Physical Location & Seq
Reverse (-)
1581834 .. 1595215
13382 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46194

Sequence Viewer

Length: 4953 bp
ATGGACGACGTGCCGCCCGATACGGGTCCTCCACCACCGCAGAGAGGTGGGGAAGAGGAGGGTCGGGCTGGGGAGTCGGGTGATGGAGCTCCGAGGCCGGATAAGGATCCGAGGAAGGTTGCCCGGAGGTATCAATTGGAGCTGTGTCAGAAAGCTGTGGAGGAGAACATAATTGTCTATTTGGAAACTGGCTGTGGAAAGACCCACATAGCAGTGTTGCTTATCTATGAGCTGCGCGAGTTGATAATGAAACCTCATAAGAACATATGTGTGTTTCTTGCGCCTACGGTGGCGCTTGTTCAACAGCAAGCAAGAGTTATAGAAGATTCCCTTGATCTCAAGGTTGGGACCTATTGTGGAAGCTCTAAGAAATTGAAAACTCACCAAGACTGGGAAAAAGAGATGGAACAATATGAGGTTTTTGTCATGACGCCTCAAATACTGTTACGTAACTTATACCACCGTATTATTAAGATGGAGTTGATTGCATTGCTGATTTTTGATGAGTGTCATCACGCTCAAATAAAAAGCAACCATCCTTACGCAGAAATTATGAAATTTTACAAGAATGATGCCACGAAACTTCCTCGTATATTTGGCATGACTGCATCTCCAGTTGTAGGTAAAGGCACTTCTAGTCAATCGAATTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATACGGTTGAAGACAAGACAGAATTGCATTGTTCTGTTGTTTCTCCTCTTATCAATGTCCATCGTTATGGTCCTGTTATTAGTGGCACATCCAGCCCCTGCATGACTTTTTCTAGAAATCTTGACCAGCTGAAACGCCAGTGTATAGCGGAACTTAGTAAGAAGACGAGTGACTACCAAAGTCTAAGGAGTACAAAGAAAACATTAAACAGAGTGCATGACAGTATAATATTTTGCTTGGAAACTCTTGGCCTTTGGGGTGCGTTACAAGCTAGACATATTCTTTCGAGTGGTGATAACTTTGAACGAAATGAATTAATAGAGGAAGGCAATAATGATATAGAGGCAGAAGGAAATAATAGTGATGACTCTGTATGTGCGGAATATCTGGCTCAGGCAGCTGACATCATTCGTACTGACTATGTGAAAGATGCTGTTGCATCTGGTCTATCTTGTGTAGAGATCTTAAAGGAGCCATTCTTCTCAAGTAAGGTCTTACGTCTTATTGGAATTCTTTCATCCTTCAGGTTGCAGCAGGATATGAAATGCATAATTTTTGTCAATAGGATTATTACTGCAAGATCCTTGTCCTACATTCTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCGAAACTGAAGAGTATGTCAAGGAAGACGATGCAGATCATTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGACTAGATATTCAGACTTGCTGCCTCGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATATATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTGGATTTAATAGAAAACTTCAGGAAAGATGAAGAGCGAATGAACATGGAAGTTGCTTTCAGAACATCCAGTGAGACTTTTCTTAGTCCTGAGGAACGAACATATAAAGTTACTTCTTCTGGAGCTTCCGTCACTTCTGGATATAGCATCTCATTGTTACACCTGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCACTCCAGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCATATAATTTTACCTTCCAATGCTCCAATGCATCAAATTGTCAGTACACCACAATCTTCAATGGAAGATGCCAAAAAAGATGCATGTCTAAAAGCCATCGAAGAATTGCATAAATTGGGTGCCTTAAGTGATTATCTCTTGCCACAGCAAGACAATGCAAATGTGGAGGAGTACATGCTAGACTCTTCTGATTATGACAGCATGGAAGATGAAGACTTACGAGAGGAACTACATGAAATGCTGGTTCCTGCTGTCCTAAAAGAGTCATGGAATAAGTTGGAGGATCTCGTCACCCTCAGCGCTTACTATTTTCAATTTGATCCTGAACCTAATGATAGAATCTATAGAAGTTTTGGTCTATTTGTCAAAGCACCTCTTCCAGCAGAGGCCGAAACAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCAGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGCTCATTCTGGACCGATCAGAATTTGTTTCTGAATTTGTTCCCCTGGGAAACTATGATTTTAGCAGATCGAGCACATCAACCTTCTACCTTTTGCTTCCAGTAACTTTGGGTGAAAATGATAAGATAAGCATAGATTGGAGGATTATCAAAAAATGTTTATCATCTCCAATATTCAGGGGTCCAGGAGATGCTATGGACAGTAAAATTATATCTTCGGGTATTCGACTTGCATCTGGTTATAGAAGCATCAGTGAAGTTGAGGATAGTTTAGTTTATGTCCCATATAAGAATACATTTTACTTCATCACTAATGTTACTCGCGAAAGAAATGCCTACAGCCAATATAAAGAAAAGTCAGATGCTTTAACCTATGTGGATCATCTAAGTAAAAAGTTTGACATTTCTCTTAAATACCCTGAGCAACATCTTTTGTGTGCAAAACCAGTTTTCAATTTGCACAACTTGCTACACAATCGTAGGCAAGAAGATTCAGAAGCACAGCAACTAGATGAGTATTATATATATGTGCCTCCTGAGCTTTGCGAGCTGAAGGTAATTGGCTTTTCAAAAGATATTGGGAGTTCGGTATCTCTGTTACCATCAATCATGCACCGTCTGGAAAACTTGCTTGTCGCCATCGAACTGAAGCATGTATTATGCACTTCATTCCCCGAAGGAGCTGAGGTTACTTCGCAGAGAGTTCTAGAAGCCCTCACAACTGAGAAGTGCCAGGAGCGGATTTCCCTTGAAAGGCTTGAAATACTAGGAGATGCTTTCCTCAAATTTGCAGTTGGAAGGCACTTTTTCCTTTTGCATGCTTCACTTGACGAAGGTGAACTAACAAGGAAACGTTCAAATGTGGTAAACAATTCCAATTTATTGAAGCTAGCTACTAGAAGCAACTTACAGGTATACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTCGCTTTAGGCCGCCCTTGCAAAAATATTTGTGACCAGGAGACAATAGGATCTCTTGATTCTCAAGATTTAAGCAGTGCAGTAAAACAGTCACATGCTCGTCACGTCAGATGTAGTAAGGGTCACCATTGGTTACATAAAAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCGGCAACTGCATTTCTCAGATGGATTGGCATAAAAGTAAACTTTGAAGCATCAGAAGTTACTAAAGTATGCATAGCAAGCAGCAGATACGTTTCACTTTCTGCTTCCATAGACATTGTGGCCCTTGAAAATTCATTAGGGTATCAGTTTCTCCACAGAGGTTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAACATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTGTATCCAAAGTTAAAGCCTGGCCATTTGACAGATTTGAGATCAATTGCTGTGAATAATAAAGCCTTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTACTCTCTGACACCAGTACCCTTTCTGAGGCTATAAAGACTTATGTGAACTTCATTGAGACATCTGCATCAGATAGTAGTCTGGTTGATGGGCCGAAATGCCCCAAGGCTCTGGGGGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGGTTTGACTTAAACCGTGTTTGGAAGATAATGCTATCCTTTCTGGAGCCTGTCATGAACTTTTCCAACATTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGATTCTTAAGTTTTCACCATCAAAGAAGGGTAAAACGTTTTCAGTTGAGGCAACAGTGAATGGGAACAATGTTTCTGCAACTGCTTCCTCAACTGCCTTGAACAAAAAGGAGGCAATTAAAATTTCTGCTCAGTTAATTTTCGAAACGTTGAAGGCTCAAGGCAATATACCAAAGTCTAAGTTGTCTTTGGAGGAAGTCTTGAAGTCAAGCTCCAAGTTGGAACCCAAACTTATCGGATATGATGAAACACCAATAGATGTTACAGCTCTTGATGTTATTGGATTTGATAAGTTAAATGTACAAGAGGCTTCTAGCAGCAGTTCCAACTCAGATGTGCACTCTATGAGTGAAGTTAGCGGTGGTAACTCCTCCTGCATAAAACGCTTACGACAACCCCCAGCTTCACTTGGAGAAGTCAAAATGGACAACACTAATGCCTGTAACAGTCGTAGCTCTGATGCTATCTCACAAAGCAGAGGTGTGCCACACAAATTAACAGCAAAATCTCGTTTGCATGAGACCTGTGTTGCTAACTTCTGGGAACCCCCTGAATTTGAATGTTGCAAAGAGGAAGGACCAGGCCACATAAAATCGTTCACTTTCAAGGTTACAGTGAAGATAGATGATGTTTCAGATACACTTTTGGAGGCCTTTGGAGAACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGGGCACTTTGGTACTTGGAAAAGAAAGGTTACATAGAGGAGAGTGATCAATCAGAGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1650

Amino Acids

185.04

Weight (kDa)

5.84

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 42 - 205 3.1e-17 Type III restriction enzyme, res subunit
DEAD PF00270 43 - 205 1.7e-18 DEAD/DEAH box helicase
Helicase_C PF00271 400 - 513 9e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 580 - 665 3.4e-23 Dicer dimerisation domain
PAZ PF02170 873 - 993 1.2e-09 PAZ domain
Ribonucleas_3_3 PF14622 1037 - 1095 5.6e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1038 - 1170 4.1e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1226 - 1357 5.7e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1246 - 1355 3.6e-21 Ribonuclease III domain
DND1_DSRM PF14709 1561 - 1636 5e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1562 - 1632 1.4e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1961, 2290
AccBSI CCGCTC 1 cut(s) 3100
AccI GTMKAC 1 cut(s) 3276
AccII CGCG 2 cut(s) 237, 2685
AclI AACGTT 3 cut(s) 3214, 4220, 4331
AclWI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
AcoI YGGCCR 1 cut(s) 3820
AcuI CTGAAG 5 cut(s) 1204, 1379, 1597, 2933, 3029
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 9 cut(s) 889, 1111, 1888, 2015, 3699, 3922, 4485, 4852, 4901
AfeI AGCGCT 1 cut(s) 2143
AfiI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
AflII CTTAAG 3 cut(s) 1304, 1966, 4190
AjiI CACGTC 2 cut(s) 10, 3418
AjnI CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
AjuI GAANNNNNNNTTGG 2 cut(s) 3468, 3500
Alw21I GWGCWC 4 cut(s) 91, 1540, 2438, 4524
Alw26I GTCTC 4 cut(s) 1661, 3347, 3956, 4697
Alw44I GTGCAC 2 cut(s) 1536, 4520
AlwI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
AlwNI CAGNNNCTG 2 cut(s) 795, 1505
Aor51HI AGCGCT 1 cut(s) 2143
AoxI GGCC 9 cut(s) 95, 946, 2229, 3322, 3636, 3820, 3995, 4765, 4833
ApaLI GTGCAC 2 cut(s) 1536, 4520
ApeKI GCWGC 9 cut(s) 232, 1094, 1228, 1473, 3597, 4500, 4865, 4868, 4880
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 2 cut(s) 1210, 2400
AspLEI GCGC 4 cut(s) 237, 283, 295, 2144
AspS9I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
AsuC2I CCSGG 1 cut(s) 124
AsuII TTCGAA 2 cut(s) 1766, 4326
AsuNHI GCTAGC 2 cut(s) 1511, 3250
AvaII GGWCC 6 cut(s) 26, 348, 767, 2374, 2543, 4760
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 3 cut(s) 1540, 4524, 4894
BaeI ACNNNNGTAYC 2 cut(s) 3904, 3937
BalI TGGCCA 1 cut(s) 3822
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 2 cut(s) 1961, 2290
BanII GRGCYC 1 cut(s) 91
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BauI CACGAG 2 cut(s) 1478, 1527
BbsI GAAGAC 4 cut(s) 714, 866, 1382, 2061
Bbv12I GWGCWC 4 cut(s) 91, 1540, 2438, 4524
BbvCI CCTCAGC 2 cut(s) 2138, 3045
BbvI GCAGC 9 cut(s) 219, 1106, 1240, 1460, 3609, 4512, 4877, 4880, 4892
BcgI CGANNNNNNTGC 2 cut(s) 2675, 2709
BciT130I CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
BciVI GTATCC 1 cut(s) 3813
BclI TGATCA 2 cut(s) 3777, 4933
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 4 cut(s) 1661, 3347, 3956, 4697
BfmI CTRYAG 2 cut(s) 2185, 2698
BfoI RGCGCY 2 cut(s) 296, 2145
BfrI CTTAAG 3 cut(s) 1304, 1966, 4190
BfuI GTATCC 1 cut(s) 3813
BglII AGATCT 3 cut(s) 1158, 2270, 2325
Bme1390I CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
Bme18I GGWCC 6 cut(s) 26, 348, 767, 2374, 2543, 4760
BmgBI CACGTC 2 cut(s) 10, 3418
BmgT120I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
BmrFI CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
BmrI ACTGGG 1 cut(s) 400
BmtI GCTAGC 2 cut(s) 1515, 3254
BmuI ACTGGG 1 cut(s) 400
BpiI GAAGAC 4 cut(s) 714, 866, 1382, 2061
BpmI CTGGAG 5 cut(s) 597, 1431, 1734, 1785, 4127
Bpu10I CCTNAGC 6 cut(s) 690, 1089, 2138, 2781, 2898, 3045
Bpu14I TTCGAA 2 cut(s) 1766, 4326
BpuEI CTTGAG 5 cut(s) 323, 1165, 2345, 3360, 4326
BpuMI CCSGG 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 3 cut(s) 105, 1385, 2253
BsaHI GRCGYC 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 4697
BsaJI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
Bse1I ACTGG 8 cut(s) 193, 395, 614, 835, 1662, 2462, 2807, 3918
Bse21I CCTNAGG 1 cut(s) 1683
Bse3DI GCAATG 1 cut(s) 488
Bse8I GATNNNNATC 3 cut(s) 105, 1385, 2253
BseBI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
BseDI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BseGI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BseJI GATNNNNATC 3 cut(s) 105, 1385, 2253
BseLI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
BseMI GCAATG 1 cut(s) 488
BseNI ACTGG 8 cut(s) 193, 395, 614, 835, 1662, 2462, 2807, 3918
BseRI GAGGAG 7 cut(s) 71, 176, 732, 2024, 3738, 4543, 4940
BseSI GKGCMC 3 cut(s) 1540, 4524, 4894
BseXI GCAGC 9 cut(s) 219, 1106, 1240, 1460, 3609, 4512, 4877, 4880, 4892
BseYI CCCAGC 2 cut(s) 68, 4582
BsgI GTGCAG 1 cut(s) 3411
Bsh1236I CGCG 2 cut(s) 237, 2685
BshFI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
BshNI GGYRCC 2 cut(s) 1961, 2290
BsiHKAI GWGCWC 4 cut(s) 91, 1540, 2438, 4524
BsiSI CCGG 2 cut(s) 98, 124
BsiWI CGTACG 1 cut(s) 4850
BslFI GGGAC 5 cut(s) 361, 1467, 1778, 2627, 4034
BslI CCNNNNNNNGG 6 cut(s) 23, 44, 391, 620, 3114, 3931
BsmAI GTCTC 4 cut(s) 1661, 3347, 3956, 4697
BsmFI GGGAC 5 cut(s) 361, 1467, 1778, 2627, 4034
BsmI GAATGC 1 cut(s) 3491
BsnI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
Bso31I GGTCTC 1 cut(s) 4697
Bsp119I TTCGAA 2 cut(s) 1766, 4326
Bsp1286I GDGCHC 5 cut(s) 91, 1540, 2438, 4524, 4894
Bsp1407I TGTACA 2 cut(s) 3697, 4483
Bsp68I TCGCGA 1 cut(s) 2685
BspANI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
BspFNI CGCG 2 cut(s) 237, 2685
BspHI TCATGA 2 cut(s) 426, 4116
BspOI GCTAGC 2 cut(s) 1515, 3254
BspPI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
BspQI GCTCTTC 1 cut(s) 1620
BspT104I TTCGAA 2 cut(s) 1766, 4326
BspT107I GGYRCC 2 cut(s) 1961, 2290
BspTI CTTAAG 3 cut(s) 1304, 1966, 4190
BspTNI GGTCTC 1 cut(s) 4697
BsrBI CCGCTC 1 cut(s) 3100
BsrDI GCAATG 1 cut(s) 488
BsrGI TGTACA 2 cut(s) 3697, 4483
BsrI ACTGG 8 cut(s) 193, 395, 614, 835, 1662, 2462, 2807, 3918
BssECI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BssNAI GTATAC 1 cut(s) 3277
BssNI GRCGYC 1 cut(s) 431
BssSI CACGAG 2 cut(s) 1478, 1527
BssT1I CCWWGG 1 cut(s) 4008
Bst1107I GTATAC 1 cut(s) 3277
Bst2BI CACGAG 2 cut(s) 1478, 1527
Bst2UI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
Bst6I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
BstACI GRCGYC 1 cut(s) 431
BstAFI CTTAAG 3 cut(s) 1304, 1966, 4190
BstAPI GCANNNNNTGC 2 cut(s) 2827, 4877
BstAUI TGTACA 2 cut(s) 3697, 4483
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 2 cut(s) 1766, 4326
BstC8I GCNNGC 8 cut(s) 309, 1513, 2334, 2909, 3180, 3252, 3595, 4878
BstEII GGTNACC 1 cut(s) 3434
BstENI CCTNNNNNAGG 1 cut(s) 3929
BstF5I GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BstFNI CGCG 2 cut(s) 237, 2685
BstH2I RGCGCY 2 cut(s) 296, 2145
BstHHI GCGC 4 cut(s) 237, 283, 295, 2144
BstMAI GTCTC 4 cut(s) 1661, 3347, 3956, 4697
BstNI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
BstNSI RCATGY 6 cut(s) 1929, 2020, 3017, 3182, 3410, 4880
BstPI GGTNACC 1 cut(s) 3434
BstSCI CCNGG 7 cut(s) 122, 2406, 2545, 3093, 3348, 3817, 4762
BstSFI CTRYAG 2 cut(s) 2185, 2698
BstSLI GKGCMC 3 cut(s) 1540, 4524, 4894
BstSNI TACGTA 1 cut(s) 449
BstUI CGCG 2 cut(s) 237, 2685
BstV1I GCAGC 9 cut(s) 219, 1106, 1240, 1460, 3609, 4512, 4877, 4880, 4892
BstV2I GAAGAC 4 cut(s) 714, 866, 1382, 2061
BstX2I RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
BstXI CCANNNNNNTGG 2 cut(s) 764, 4015
BstYI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
BstZ17I GTATAC 1 cut(s) 3277
Bsu36I CCTNAGG 1 cut(s) 1683
BsuI GTATCC 1 cut(s) 3813
BsuRI GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
BtrI CACGTC 2 cut(s) 10, 3418
BtsCI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BtsI GCAGTG 2 cut(s) 219, 3394
BtsIMutI CAGTG 9 cut(s) 219, 842, 1582, 1669, 2620, 3394, 3804, 4245, 4803
BtuMI TCGCGA 1 cut(s) 2685
Cac8I GCNNGC 8 cut(s) 309, 1513, 2334, 2909, 3180, 3252, 3595, 4878
CaiI CAGNNNCTG 2 cut(s) 795, 1505
CciI TCATGA 2 cut(s) 426, 4116
CfoI GCGC 4 cut(s) 237, 283, 295, 2144
Cfr13I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
CseI GACGC 1 cut(s) 439
Csp6I GTAC 9 cut(s) 888, 1110, 1887, 2014, 3698, 3921, 4484, 4851, 4900
CviQI GTAC 9 cut(s) 888, 1110, 1887, 2014, 3698, 3921, 4484, 4851, 4900
DraI TTTAAA 1 cut(s) 3514
EaeI YGGCCR 1 cut(s) 3820
Eam1104I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
EarI CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
Ecl136II GAGCTC 1 cut(s) 89
Eco105I TACGTA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 4008
Eco147I AGGCCT 1 cut(s) 4835
Eco24I GRGCYC 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 4697
Eco47I GGWCC 6 cut(s) 26, 348, 767, 2374, 2543, 4760
Eco47III AGCGCT 1 cut(s) 2143
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 5 cut(s) 1204, 1379, 1597, 2933, 3029
Eco81I CCTNAGG 1 cut(s) 1683
Eco91I GGTNACC 1 cut(s) 3434
EcoICRI GAGCTC 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 3929
EcoO109I RGGNCCY 2 cut(s) 26, 348
EcoO65I GGTNACC 1 cut(s) 3434
EcoRI GAATTC 2 cut(s) 1206, 1805
EcoRII CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
EcoT14I CCWWGG 1 cut(s) 4008
EcoT22I ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 4008
FalI AAGNNNNNCTT 6 cut(s) 315, 347, 2202, 2234, 4878, 4910
FaqI GGGAC 5 cut(s) 361, 1467, 1778, 2627, 4034
FauNDI CATATG 1 cut(s) 266
FbaI TGATCA 2 cut(s) 3777, 4933
FblI GTMKAC 1 cut(s) 3276
FokI GGATG 6 cut(s) 522, 772, 1201, 1555, 1645, 3687
FriOI GRGCYC 1 cut(s) 91
GlaI GCGC 4 cut(s) 236, 282, 294, 2143
GsaI CCCAGC 2 cut(s) 72, 4586
GsuI CTGGAG 5 cut(s) 597, 1431, 1734, 1785, 4127
HaeII RGCGCY 2 cut(s) 296, 2145
HaeIII GGCC 9 cut(s) 97, 948, 2231, 3324, 3638, 3822, 3997, 4767, 4835
HapII CCGG 2 cut(s) 98, 124
HgaI GACGC 1 cut(s) 439
HhaI GCGC 4 cut(s) 237, 283, 295, 2144
Hin1I GRCGYC 1 cut(s) 431
Hin6I GCGC 4 cut(s) 235, 281, 293, 2142
HinP1I GCGC 4 cut(s) 235, 281, 293, 2142
HincII GTYRAC 1 cut(s) 3502
HindII GTYRAC 1 cut(s) 3502
HindIII AAGCTT 1 cut(s) 1769
HpaII CCGG 2 cut(s) 98, 124
Hpy99I CGWCG 1 cut(s) 11
HpyCH4IV ACGT 8 cut(s) 9, 448, 1195, 3214, 3417, 3606, 4220, 4331
HpySE526I ACGT 8 cut(s) 9, 448, 1195, 3214, 3417, 3606, 4220, 4331
Hsp92I GRCGYC 1 cut(s) 431
HspAI GCGC 4 cut(s) 235, 281, 293, 2142
Ksp22I TGATCA 2 cut(s) 3777, 4933
LguI GCTCTTC 1 cut(s) 1620
Lsp1109I GCAGC 9 cut(s) 219, 1106, 1240, 1460, 3609, 4512, 4877, 4880, 4892
MaeII ACGT 8 cut(s) 9, 448, 1195, 3214, 3417, 3606, 4220, 4331
MbiI CCGCTC 1 cut(s) 3100
MfeI CAATTG 3 cut(s) 134, 3459, 3843
MflI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
MhlI GDGCHC 5 cut(s) 91, 1540, 2438, 4524, 4894
MlsI TGGCCA 1 cut(s) 3822
MluNI TGGCCA 1 cut(s) 3822
MlyI GAGTC 6 cut(s) 83, 1058, 1353, 2018, 2114, 4040
MmeI TCCRAC 7 cut(s) 1575, 2100, 3136, 3466, 4152, 4383, 4533
Mox20I TGGCCA 1 cut(s) 3822
Mph1103I ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
MroXI GAANNNNTTC 2 cut(s) 1210, 2400
MscI TGGCCA 1 cut(s) 3822
MslI CAYNNNNRTG 3 cut(s) 212, 269, 762
Msp20I TGGCCA 1 cut(s) 3822
MspA1I CMGCKG 4 cut(s) 826, 1097, 3507, 4883
MspCI CTTAAG 3 cut(s) 1304, 1966, 4190
MspI CCGG 2 cut(s) 98, 124
MspR9I CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
MunI CAATTG 3 cut(s) 134, 3459, 3843
Mva1269I GAATGC 1 cut(s) 3491
MvaI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
MvnI CGCG 2 cut(s) 237, 2685
NciI CCSGG 1 cut(s) 124
NdeI CATATG 1 cut(s) 266
NheI GCTAGC 2 cut(s) 1511, 3250
NmuCI GTSAC 8 cut(s) 866, 1524, 1723, 2131, 3344, 3402, 3413, 3434
NruI TCGCGA 1 cut(s) 2685
NsiI ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
NspI RCATGY 6 cut(s) 1929, 2020, 3017, 3182, 3410, 4880
NspV TTCGAA 2 cut(s) 1766, 4326
PaeI GCATGC 2 cut(s) 3182, 4880
PagI TCATGA 2 cut(s) 426, 4116
PasI CCCWGGG 1 cut(s) 2407
PceI AGGCCT 1 cut(s) 4835
PciSI GCTCTTC 1 cut(s) 1620
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 3491
PdmI GAANNNNTTC 2 cut(s) 1210, 2400
PfeI GAWTC 7 cut(s) 326, 1309, 1420, 2181, 2852, 3371, 4186
Pfl23II CGTACG 1 cut(s) 4850
PfoI TCCNGGA 1 cut(s) 2545
PleI GAGTC 6 cut(s) 82, 1058, 1352, 2018, 2113, 4039
PpsI GAGTC 6 cut(s) 82, 1058, 1352, 2018, 2113, 4039
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 2 cut(s) 26, 348
PshBI ATTAAT 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 91
Psp1406I AACGTT 3 cut(s) 3214, 4220, 4331
Psp5II RGGWCCY 2 cut(s) 26, 348
Psp6I CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
PspEI GGTNACC 1 cut(s) 3434
PspFI CCCAGC 2 cut(s) 68, 4582
PspGI CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
PspLI CGTACG 1 cut(s) 4850
PspPI GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
PspPPI RGGWCCY 2 cut(s) 26, 348
PstNI CAGNNNCTG 2 cut(s) 795, 1505
PsuI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
PvuII CAGCTG 2 cut(s) 826, 1097
RruI TCGCGA 1 cut(s) 2685
RsaI GTAC 9 cut(s) 889, 1111, 1888, 2015, 3699, 3922, 4485, 4852, 4901
RsaNI GTAC 9 cut(s) 888, 1110, 1887, 2014, 3698, 3921, 4484, 4851, 4900
RseI CAYNNNNRTG 3 cut(s) 212, 269, 762
SacI GAGCTC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 1620
Sau96I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
SchI GAGTC 6 cut(s) 83, 1058, 1353, 2018, 2114, 4040
ScrFI CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
SduI GDGCHC 5 cut(s) 91, 1540, 2438, 4524, 4894
SfcI CTRYAG 2 cut(s) 2185, 2698
SfuI TTCGAA 2 cut(s) 1766, 4326
SinI GGWCC 6 cut(s) 26, 348, 767, 2374, 2543, 4760
SmiMI CAYNNNNRTG 3 cut(s) 212, 269, 762
SmlI CTYRAG 8 cut(s) 338, 1180, 1304, 1966, 2360, 3375, 4190, 4341
SmoI CTYRAG 8 cut(s) 338, 1180, 1304, 1966, 2360, 3375, 4190, 4341
SnaBI TACGTA 1 cut(s) 449
SphI GCATGC 2 cut(s) 3182, 4880
SseBI AGGCCT 1 cut(s) 4835
SspI AATATT 3 cut(s) 927, 2535, 3340
SstI GAGCTC 1 cut(s) 91
StuI AGGCCT 1 cut(s) 4835
StyD4I CCNGG 7 cut(s) 122, 2406, 2545, 3093, 3348, 3817, 4762
StyI CCWWGG 1 cut(s) 4008
TaiI ACGT 8 cut(s) 12, 451, 1198, 3217, 3420, 3609, 4223, 4334
TaqII GACCGA 2 cut(s) 1395, 2391
TatI WGTACW 5 cut(s) 887, 1886, 2013, 3697, 4483
TauI GCSGC 3 cut(s) 16, 3327, 3521
TfiI GAWTC 7 cut(s) 326, 1309, 1420, 2181, 2852, 3371, 4186
TscAI CASTG 9 cut(s) 219, 842, 1582, 1669, 2620, 3394, 3804, 4245, 4803
TseFI GTSAC 8 cut(s) 866, 1524, 1723, 2131, 3344, 3402, 3413, 3434
TseI GCWGC 9 cut(s) 232, 1094, 1228, 1473, 3597, 4500, 4865, 4868, 4880
Tsp45I GTSAC 8 cut(s) 866, 1524, 1723, 2131, 3344, 3402, 3413, 3434
TspGWI ACGGA 2 cut(s) 1555, 1711
TspRI CASTG 9 cut(s) 219, 842, 1582, 1669, 2620, 3394, 3804, 4245, 4803
Vha464I CTTAAG 3 cut(s) 1304, 1966, 4190
VneI GTGCAC 2 cut(s) 1536, 4520
VpaK11BI GGWCC 6 cut(s) 26, 348, 767, 2374, 2543, 4760
VspI ATTAAT 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 3929
XbaI TCTAGA 2 cut(s) 809, 3067
XceI RCATGY 6 cut(s) 1929, 2020, 3017, 3182, 3410, 4880
XcmI CCANNNNNNNNNTGG 1 cut(s) 3631
XmiI GTMKAC 1 cut(s) 3276
XmnI GAANNNNTTC 2 cut(s) 1210, 2400
Zsp2I ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.